We isolated polyA~+ mRNA direcdy from tumor tissue of ovarian carcinoma using Oligotex~(TM) Direct mRNA Kit (QIAGEN) to synthesize the first and second strand cDNA. The ds-cDNA termini were blunted with pfu DNA poly-rnerase. The blunted cDNAs were added EcoR I adaptor, and then were digested by Xho I . Small cDNA molecules(

Objective To understand the quality of life in patients with chronic obstructive pulmonary disease (COPD) ,and to analyze the influencing factors for their quality of life .Methods A total of 250 patients with COPD from 3 hospitals in Zhanjiang City were investigated by using the Chinese Scale Of World Health Organization (WHOQOL‐BREF) ,and the influencing factors for quality of their life were analysed ,as well .Results A total of 237 qualified questionnaires were reclaimed out of 250 questionnaires , and the response rate was 94 .80% .Scores of physiological aspect ,psychological aspect ,social relation ,environmental condition and comprehensive health self evaluation were (59 .57 ± 15 .73) ,(56 .99 ± 13 .01) ,(64 .28 ± 14 .82) ,(58 .70 ± 11 .53)and (74 .97 ± 12 .61) ,respectively .The scores of quality of life in the physiological aspect ,psychological aspect ,social relation and environmental condition of male patients with COPD were higher than those of the female ;the scores of quality of life in the social relation in pa‐tients with higher education level were higher than that in patients with lower education level ,the scores of quality of life in the physiological aspect ,psychological aspect ,social relation and environmental condition in patients with higher income level were high‐er than those in patients with lower income level ;the disease severity was higher ,the scores of quality of life in the physiological as‐pect ,psychological aspect ,social relation and environmental condition were lower ,all had statistically significant differences (P<0 .05) .Logistic regression analysis showed that sex ,monthly income level and disease severity were the influence factors of quality of life self evaluation in COPD patients .Conclusion The quality of life in patients with COPD might be influenced by sex ,income level ,level of education and disease severity .It is necessary to strengthen the health education ,pay attention to personalized care and improve the quality of life of patients with COPD .

Objective To screen the biomarkers in nasal lavage fluid of patients with allergic rhinitis (AR) by proteomics tech‐nology .Methods The nasal lavage fluid samples of 10 cases of AR patients and 20 cases of normal controls were collected both be‐fore and after allergen stimulation tests .Two‐dimensioned gel eletrophoresis was used to detect the proteins in nasal lavage fluid samples .The proteins that showed different expression levels were identified by MALDI‐TOF/TOF mass spectrometry .Results Six kinds of proteins differentially expressed were screened out .The expressions of lactoferrin ,α1‐antitrypsin ,and APOA1 increased in the AR patients after being stimulated by allergens .On the contrary ,the expressions of PLUNC protein ,haptoglobin and IgJ de‐creased .Conclusion The six kinds of proteins might be the biomarkers of AR .

Objective: Full expression of class I HLA molecules on tumor cells is pivotal in priming the tumor antigen-specific CTLs for effective iramunotherapies. Tumor cells display abnonnal expression of HLA class I molecules in human ovarian carcinoma. Methods: In this study, the expression of class I molecules in human ovarian cancer cells was determined by using Western bloting, immunohistochemistry testing and flow cytometry. The mRNA of TAP (transporter associated with antigen processing) and LMP(low molecular weight polypeptide) were exannined at the same time by RT-PCR. Results: It has been shown that class I molecules were unable to be, or only weakly, expressed in five of eight ovarian cancer cell lines. On these cell lines abnormal in class I expression, no or only little mRNA were detected for TAP1, TAP2, LMP2 and(or) LMP7 genes. Class I expression, however, could be partially recovered by incubating the tumor cells at 25℃ when compared with those at 37℃ . Conclusion: The results suggested that the dysfunction of TAP and IMP genes, following by a defective expression of class I molecules, might be one of the mechanisms enable ovarian cancer cells to escape from immune surveillance.

Objective: Hyperglycemia is common during the early stage of acute myocardial infarction(AMI),which has potential implications for the outcomes of AMI.This study intends to investigate the clinical significance of hyperglycemia in non-diabetic patients with AMI.Methods: The clinical data of 83 non-diabetic patients with AM1 were collected and divided into 3 groups based on the fasting blood glucose(FBG) level at admission: normal group(FBS

Objective:To clone HLA A *0201 cDNA and express it transiently on COS 7 cells.Methods:HLA A cDNA isolated from the HLA A *0201 positive lymphocytes by using RT PCR was cloned into pBluescript II SK vector directionally.After the sequence was confirmed,the cDNA was inserted into mammalian expression vector pcDNA3.The recombinant vector was transfected into COS 7 cells by cation liposome.The transient expression on the cells was measured by flow cyteometer.Results:The cDNA was identical with HLA A *0201 cDNA published on Genebank.Determined by flow cytemeter,the expressing rate was recorded for 57%.Conclusion:We have cloned HLA A *0201 successfully and expressed it transiently on COS 7 cell,which would be potentially useful in research on killing tumor restricted by HLA A2.

Patients with acute myocardial infarction(AMI) are often with elevated blood glucose level when admitted to hospital,this finding is associated with poor prognosis regardless of a history of diabetes.Though numerous studies have addressed some of the questions in this field,many critically important questions are still poorly understood and under debate.This article summarized current findings in hyperglycemia and its potential link withadverse outcomes in AMI patients,and addressed some of the existing controversies in this field.

OBJECTIVE: To investigate the effects of Gypsum Fibrosum and Gypsum Fibrosum Preparatum in promoting granulation. METHODS: The wounds of muscle layer were produced in rats by using surgical operation. Two round wounds, with diameter about 1.5 cm, were cut at the depilatory area of two sides of the back of each rat, with an interval of 2 cm, deep to muscle layer, and the thickness of the knife wound of muscle layer was about 0.15 cm. Forty SD rats with the wounds were randomly divided into 4 groups: untreated group, beifuji-treated group, Gypsum Fibrosum-treated group and Gypsum Fibrosum Preparatum-treated group, with 10 rats in each group. Then the wounds were sprinkled with powders of Gypsum Fibrosum and Gypsum Fibrosum Preparatum, or sprayed with beifuji solution, respectively. The healing state of granulation tissues of the wounds was observed at the eighth and fourteenth day respectively. RESULTS: The number of fibroblasts, the number of capillary tubes and the area of capillary tubes in granulation tissue of wounds in the Gypsum Fibrosum Preparatum-treated group were significantly higher than those in the untreated group and Gypsum Fibrosum-treated group (P<0.01). There were no statistical differences between the Gypsum Fibrosum Preparatum-treated group and the beifuji-treated group. However, Gypsum Fibrosum-treated group showed no obvious differences compared to the untreated group (P>0.05). CONCLUSIONS: Gypsum Fibrosum Preparatum can accelerate the formation of collagenoblast and micrangium in wounds, and the proliferation of granulation tissues, thus promoting the skin wounds to healing. The effect of Gypsum Fibrosum is changed after being calcined, and Gypsum Fibrosum Preparatum has obvious effect in promoting granulation.

With the resource based relative value scale (RBRVS) application at a hospital cited as an example, the paper covered its use in performance appraisal for optimal performance-based distribution as follows. In view of hospital specifics, such appraisal indexes as the quantity, quality, technical difficulty and cost control are quantified as key indicators, to form its own RBRVS scores and weights. In combination of the case mixed index as well, such factors as specific appraisal, quality of care and research/teaching work are taken into account for the purpose of rational and optimal pay for performance.

Objective To learn the initial application of RBRVS related performance appraisal at public hospitals in China. Methods 12 tertiary public hospitals which took the lead to introduce RBRVS models, via third-party cooperation, in medical stuff performance assessment over one year were surveyed. The study summarized their design emphases of RBRVS performance appraisal, covering such aspects as appraisal level, accounting method, conceptual design, points setup and items charged as cost. Descriptive statistics was used to analyze the data so acquired. Results As shown in the performance appraisal level, most hospitals were found with defects in refining levels, as eight hospitals only appraise down to the department level, three to diagnostic group level, and only one hospital to individuals. Of these hospitals, over eight hospitals used RBRVS to elevate the efficiency and inventiveness of employees, while half of these hospitals reported the method can improve service, reduce cost and enhance quality of care. Conclusions Public hospitals enjoy advantages via third-party cooperation RVRBS method in RVRBS-related reforms. Introduction of this method calls for localization design based specifics and management goals of public hospitals.