Objective To investigate the effect of Xuezhokang treatment on blood pressure and pulse pressure(PP) of elderly patients with primary hypertension(PH). Methods Fifty-eight elderly PH patients were treated with Amlodipine(5 mg/d).After the blood pressure were decreased to mild hypertension level,all the patients were randomly divided into 2 groups:30 patients in the Xuezhikang group,and 28 patients in the control group.The patients in the control group were treated with low fat and low salt diet and Amlodipine as before,and the patients in Xuezhikang group were treated not only with Xuezhikang 300 mg twice a day and Amlodipine as before for 12 weeks.Results The SBP and PP(mm Hg)were improved in both Xuezhikang and control groups,and Xuezhikang showed a better therapeutic effect(P＜0.05)than did the control.Xuezhikang group had SBP of(149±9,pretreatment)and(130±8,posttreatment),and PP of(64±11,pretreatment)and (48±7,posttreatment)(all P＜0.05).Control group showed SBP of(144±9)and(130±8)in pre-and post-treatment respectively,and showed PP of(60±10,pre-Rx)and(48±7,post-Rx)(all P＜0.05). Conclusions Anti-hypertensive medications in a routine dose combined with Xuezhikang have a better effect on the hypertension treatment in elderly patients.

Objective Amblyopia is common disease in children. It can cause visual suppression that damage binocular vision function especially to the development of stereopsis. The objective of this study was to investigate the stereoscopic vision in children with different types and degrees of amblyopia. Methods This is a clinical retrospective comparative analysis. 178 children with amblyopia from Sichuan Provincial Corps Hospitial CAPF were enrolled in this study. Approximation zero disparity, crossed disparity, uncrossed disparity were examined with Yan' s stereogram. The classification of stereogram based on the criteria of Okuda. The degree of amblyopia was graded based on the corrected vision acuity. Results The differences in the eyes and percentage of approximation zero disparity, crossed disparity, uncrossed disparity among refractive amblyopia group, anisopia amblyopia group and strabismic amblyopia group were statistically significant, respectively(χ~2 =13. 979, P =0. 001; χ~2 = 15. 198, P = 0. 001 ; χ~2 =6. 974, P = 0. 031) . Approximation zero disparity, crossed disparity in mild amblyopia were better than of medium amblyopia in ametropic amblyopic children (P ＜ 0. 05) . No statistically significant difference in the eye number and percentage of uncrossed disparity was found between mild and moderate refractive ametropia (P ＞ 0. 05) . The same outcomes also were seen in approximation zero disparity, crossed disparity, uncrossed disparity between mild and medium anisopia amblyopia (P ＞ 0. 05) . No statistically significant differences in the eye number and percentage of approximation zero disparity, crossed disparity were found between mild and moderate strabismic amblyopia (P ＞0. 05) . Conclusion Amblyopia affects the development of stereoacuity. Amblyopia is aggravated with the increase of amblyopia degrees. The influence of amblyopia on stereoscopic vision is most mild in refractive ametropic and most serious in strabismic amblyopia.

Objective:To assess the advantages and nursing experience of foley catheter for nephrostomy tube after minimally invasive percutaneous nephrolithotomy (mPCNL).Methods:From October 2015 to April 2016,the clinical data of 137 patients,who were diagnosed with upper urinary tract calculi and need to perform mPCNL,were collected and randomized into 2 groups:a foley catheter group (research group,n=69) and a normal nephrostomy tube group (control group,n=68).The patients in research group used foley catheter for nephrostomy tube,while those in the control group used normal nephrostomy tube.Bleeding volume,the days of bleeding,pipes shedding and pain degree were compared,and the experience of the nursing process was summarized.Results:The bleeding volume,the days of bleeding in the research group were significantly lower than those in the control group (both P＜0.01).The pipes shedding rate in the research group were lower than that in the control group (P＜0.05).There was no significant difference in postoperative pain scores between the 2 groups (P＞0.05).There was no other complications and discomfortable symptoms in the experimental group.Conclusion:The use of foley catheter for nephrostomy tube after mPCNL is safe,and it can decrease the bleeding volume and pipes shedding rate.It doesn't increase the patient's postoperative pain and can reduce the difficulty and risk for postoperative nursing.

Objective To investigate the reference ranges of peripheral white blood cells (WBC) and five classification parame‐ters among 13 442 apparently healthy children to provide reference for clinical diagnosis .Methods A total of 13 442 apparently healthy children aged from 1 month old to 5 years undergoing the health physical examination in the healthcare department of the Guangzhou Municipal Women and Children Medical Center were chosen according to the healthy children screening standard and the exclusion standard of hematological diseases ,infection diseases and drug administration history .The WBC analysis and its parame‐ters detection were performed by using the Sysmex XS‐800i automatic blood cell analyzer .Results WBC and its five classification parameters were not accorded with the normally distribution .By the Mann‐whitney test ,no statistically significant differences in WBC ,BASO# and NEU # were detected between different genders of children (P> 0 .05) ,while the differences in LYM # , MONO# and EOS# had statistical significance(P<0 .001) ,in which the LYM # level in females was significantly higher than that in males ,while the MONO# and EOS# levels in females were lower than those in males ;By the Kruskal‐Wallis test ,WBC and five classification parameters had statistically significant differences among different age groups (P<0 .001) .The parameters of WBC ,LYM # ,MONO# and EOS# were decreased with age increase ,while NEU # was constantly increased with age increase and BASO# was decreased with age increase before 4 years old .Conclusion All factors such as gender ,age should be The inde‐pendent reference rangesl of WBC parameters among children aged from 1 month old to 7 years old should be formulated on the ba‐sis of fully considering the factors such as gender ,age .

Objective To explore the clinical significance of platelet count increasing in patients with ovarian malignant tumor . Methods 80 cases of ovarian malignant tumor patients ,80 patients with ovarian benign tumor and 80 cases of healthy women were enrolled in the study as group A ,B ,C respectively .The group A was divided into FIGOⅠ group(n=30) ,and FIGOⅡ and above group(n=50) .Platelet count was detected by using fully automatic blood cell analyzer and the serum CA125 concentrations were also measured for all the groups .Results The platelet count and CA125 concentrations in group A were higher than group B and group C(P< 0 .05) .The positive rate of platelet increasing and serum CA125 were also higher than group B and group C(P<0 .05) .There were significant differences between FIGOⅠ group and FIGOⅡ and above group in platelet count and CA125 concen‐trations .Conclusion Ovarian malignant tumor complicated with increased platelet count is common in patients of advanced stage . The increased platelet count could indicate the malignant degree of ovarian malignant tumor .

The xylose reductase of Pichia stipitis is one of the most important enzymes. It can be used to build up recombinant Saccharomyces cerevisiae strain for utilizing xylose and producing ethanol. Intercellular redox imbalance caused by NADPH preference over NADH for Pichia stipitis xylose reductase (PsXR) has been considered to be one of the main factors for poor ethanol productivity. Some key amino acids of PsXR, which affect the activity or coenzyme preference, were investigated in our previous study. In this study, Lys21 were rational designed for site-directed mutagenesis to alter coenzyme specificity of PsXR from NADPH and NADH into single NADH. The wild gene and mutagenesis genes were ligated into pET28b, and were transferred into E.coli BL21(DE3). After induced by IPTG, the xylose reductases were purified. Purified mutants K21A (Lys21-->Ala), K21R(Lys21-->Arg) were characterized by steady-state kinetic analysis. The results showed that the coenzyme dependence of K21A was completely reversed to NADH.
Aldehyde Reductase ; metabolism ; Amino Acid Substitution ; genetics ; Coenzymes ; pharmacology ; Escherichia coli ; genetics ; metabolism ; Ethanol ; pharmacology ; Lysine ; genetics ; Mutagenesis, Site-Directed ; NAD ; metabolism ; NADP ; metabolism ; Pichia ; chemistry ; genetics ; metabolism ; Recombinant Proteins ; biosynthesis ; genetics ; metabolism ; Recombination, Genetic ; Xylose ; pharmacology

Objective To investigate the variation characteristics of peripheral platelet parameters in children with dengue fever and to assess their use and value in diagnosis,prognosis and treatment for dengue fever.Methods Platelet parameters of 69 pediatric patients with dengue fever in acute febrile phase and remission period and 103 healthy controls were compared.The difference of platelet parameters between the liver function injury group and the normal liver function group,the coagulation dysfunction group and the coagulation function normal group,the severe group and the mild group with dengue fever was compared,the causes of thrombocytopenia in children with dengue was investigated so as to better understand the situation of bone marrow producing platelets.Results P-LCR,PDW,MPV were significantly higher in patients in acute febrile phase of dengue fever,compared with the healthy subjects(P＜0.01),while PCT,PLT were significantly lower in patients in acute febrile phase of dengue fever,compared with the healthy subjects(P＜0.01).MPV was significantly lower in patients with abnormal hepatic function when compared with patients with normal hepatic function(P＜0.05),while other platelet parameters did not show significant differences(P＞0.05).PLT and PCT were significantly lower in the severe patients than those in the mild patients(P＜0.05),while other platelet parameters did not show significant differences(P＞0.05).Peripheral platelet parameters did not show significant differences between patients with or without abnormal coagulation function (P＞0.05).Conclusion Peripheral platelet parameters may play a role in the diagnosis,treatment and prognosis of dengue fever in children.

Objective To explore the effect of comprehensive nursing intervention in patients with acute myocardial infarction (AMI).Methods A total of 100 patients with acute myocardial infarction were randomly divided into two groups.The patients in the control group were given routine nursing and the patients in the experimental group were given comprehensive nursing intervention.Angina severity,quality of life,psychological status,incidence of adverse cardiac events and family care satisfaction before and after intervention were compared.Results Compared with the control group,the degree of physical activity limitation,angina pectoris stabilized status,angina pectoris attack status,treatment satisfaction and disease cognition score of the experimental group were significantly higher than that of the control group.After the intervention,the physiological function,physiological function,social function,emotional function,energy,mental health and general health status scores were significantly higher than the control group;Anxiety and depression scores were significantly lower than the control group;The overall incidence of adverse cardiac events was significantly lower (P ＜ 0.01).Conclusion The comprehensive nursing intervention has a significant effect in the first aid of acute myocardial infarction patients,which can improve the satisfaction of the family members.

Objective To analyze the influencing factors of vaginal birth after cesarean (VBAC) in re-pregnancy puerperae and mother-infant outcomes.Methods A total of 290 re-pregnant women after cesarean were enrolled in the study,and 210 pregnant women were successfully given vaginal delivery,among whom 180 cases with vaginal birth were selected as the observation group,and 80 cases with cesarean section were as control group.The factors affecting the success of vaginal delivery after cesarean section were analyzed,and the maternal and neonatal outcomes between the two groups were compared.Results The univariate analysis showed that fetal head position,fetal abdominal circumference,fetal biparietal diameter,uterine height,premature rupture of membranes,Bishop score,and epidural anesthesia of two groups were statistically significant(P ＜ 0.05);Multivariate logistic regression analysis showed that Bishop score,fetal abdominal circumference,fetal head position,and epidural anesthesia were independent influencing factors of VBAC success (P ＜ 0.05).The incidence rates of uterine rupture,the rate of neonatal asphyxia,hospitalization rate,Apgar score of two groups showed no significant difference (P ＞ 0.05).The bleeding rates at 2,24 h in the observation group were lower than that in the control group (P ＜ 0.05).Conclusion There are various factors that affect the success of vaginal delivery after cesarean section,and prenatal examination should be scientifically evaluated.If there is no indication of cesarean section,maternal vaginal delivery should be guided to improve the maternal and child outcomes and life quality of mother and child.

Objective To explore the effect of comprehensive nursing intervention in patients with acute myocardial infarction (AMI).Methods A total of 100 patients with acute myocardial infarction were randomly divided into two groups.The patients in the control group were given routine nursing and the patients in the experimental group were given comprehensive nursing intervention.Angina severity,quality of life,psychological status,incidence of adverse cardiac events and family care satisfaction before and after intervention were compared.Results Compared with the control group,the degree of physical activity limitation,angina pectoris stabilized status,angina pectoris attack status,treatment satisfaction and disease cognition score of the experimental group were significantly higher than that of the control group.After the intervention,the physiological function,physiological function,social function,emotional function,energy,mental health and general health status scores were significantly higher than the control group;Anxiety and depression scores were significantly lower than the control group;The overall incidence of adverse cardiac events was significantly lower (P ＜ 0.01).Conclusion The comprehensive nursing intervention has a significant effect in the first aid of acute myocardial infarction patients,which can improve the satisfaction of the family members.