Objective To detect the expression of integrin ?5?1 in restenosed human vein grafts.Methods The expression of integrin ?5?1 and ?-smooth muscle actin(?-SMA) in 30 resected restenosed human vein grafts(from Kerfuff clinical hospital of Germany) was detected by confocal immunofluorescence with specific antibodies against ?5?1 and ?-SMA.Images were processed with Silicon Graphics Octane.Results In normal veins,integrin ?5?1 expression was very weak in media smooth muscle cells and in endothelium,and ?-SMA expression was present in media smooth muscle cells.In the restenosed vein grafts,integrin ?5?1 was strongly stained in the media smooth muscle cells and intimal endothelial cells,and moderately in the intimal smooth muscle cells,?-SMA was present in media smooth muscle cells and in the intimal smooth muscle cells.Conclusion Our research reveals that integrin ?5?1 is significantly upregulated in the media smooth muscle cells and intimal endothelial cells in the restenosed human vein grafts,suggesting the participation of integrin ?5?1 in the restenosis formation of human vein grafts.

Objective To observe the effect of robot-assisted upper limb training on the recovery of upper limb motor function after stroke.Methods One hundred patients within one month of suffering a stroke were randomly divided into a control group (50 cases) and a therapy group (50 cases).Both groups were given conventional drug therapy and rehabilitation training,while the therapy group also received robot-assisted training once a day,5 days per week for 12 weeks.The Fugl-Meyer assessment for the upper extremities (FMA-UE),the modified Ashworth scale (MAS) and the modified Barthel index (MBI) were used to evaluate the subjects before the experiment and after 4,8 and 12 weeks of treatment.Results During the treatment,the average FMA-UE scores and the average MBI of both groups increased gradually.The treatment group's averages were significantly better than those of the control group at each time point,except that there was no significant difference in elbow and wrist MAS scores.Conclusion Upper limb robots can be used with acute stroke patients in a clinical setting,and they may be beneficial for improving upper limb function and ability in the activities of daily living.

Objective:To establish a method to detect food allergen based on Latex-enhanced immune turbidimetry ( PETIA) and apply to clinic.Methods: The PETIA method was used for evaluating the detection system including standard curve , detection-limit,stability, intra-assay and inter-assay precision and cross-reactivity,clinical normal serum specimens and clinical allergic diseases serum specimens were measured to evaluated the perspective of the clinical application .Results: The standard curve :Y=837.1 x2-125.2x+10.036, linear range: 0.0-400 U/ml, the correlation coefficient of the standard curve was 0.996, the intrabatch and interbatch precision was<10%.The normal reference range was≤33.5 U/ml,the AUC of ROC curve was 0.957,the sensitivity was 89.61%,The specificity was 65.22%, the accuracy was 84.00%, and the positive predicted value was 89.61%, the negative predicted value was 65.22%.the test results have strong correlation with ELISA ( r=0.890 2 ) .Conclusion: The PETIA method for detecting food allergen achieved corresponding clinical application standards and may be used for the diagnosis of food allergies .

OBJECTIVE:To study the hypoglycemic effects of Insulin self-microemulsion for parenteral administration on mod-el rats with type 1 diabetes in vivo. METHODS:Rats were treated with streptozotocin(50 mg/kg)to reproduce model with type 1 diabetes,ip. The model rats were randomly divided into model group (normal saline),positive control group (Insulin injection 2.25 u/kg) and self-microemulsion low,medium and high dose groups (Insulin self-microemulsion 4.5,9 and 18 u/kg);and 10 normal rats were involved in sham-operation group (normal saline). Anesthesia and operation were conducted for all rats. Positive control group was administrated,ip;other rats were parenterally administrated. The blood glucose levels in groups were detected be-fore and after 15-600 min administration. Glucose tolerance test was conducted for the rats in normal control group,model group without glucose,model group with glucose and microemulsion group (Insulin self-microemulsion 9 u/kg). All group were given glucose except model group without glucose. RESULTS:Compared with sham-operation group,the blood glucose levels in model group within 0-240 min were increased,with significant difference(P<0.05). Compared with model group,there was hypoglyce-mic trend in positive control group after 15 min,the blood glucose levels within 30-480 min were decreased,the hypoglycemic peak was 36%,and the peak time was 30 min;there was also hypoglycemic trend in microemulsion low,medium and high dose groups after 30 min,the blood glucose levels within 45-360 min were decreased,hypoglycemic peak was 18%-21%,and the peak time was 90-120 min,with significant differences (P<0.01 or P<0.05). All rats had glucose absorption peak except for model group without glucose in glucose tolerance test,and glucose of rats in microemulsion group reached its peak and then quickly de-creased. CONCLUSIONS:Insulin self-microemulsion can obviously reduce the blood glucose of model rats with type 1 diabetes.

Improving the special nursing ability is an important guarantee to carry out the clinical work for specialized nurse, so it is necessary to receive special hierarchical training.According to the length of employment and position, all nurse staff in the Department of Hepatobiliary Surgery ,Children's Hospital of Chongqing Medical University were classified, and the training schedule was made according to the level of personnel development to implement specialist training and operational skills training and assessment.And then examination was administrated;individualized guidance was got across to everyone according to the outcome of examination.Before and after the hierarchical training,analysis of doctors' and patients' satisfaction for nursing work, nursing theory, nursing service quality was carried out.The aim of this study is to verify the positive role of hierarchical training mode in promoting individual nursing ability and improve the level of nursing team, to provide experience and reference for nursing managers.

OBJECTIVE:To extract volatile rose oil by the supercritical fluid extraction technology.METHODS:The optimum extraction technology condition was investigated by orthogonal experiment with extraction rate as the evaluation in?dex,and with the pressure,temperature of extraction and the flow of CO 2 as investigation factors(3levels of each were cho?sen).RESULTS:The optimum technology condition was the following:the extraction pressure was25MPa,the temperature was50℃and the flux of CO 2 was600L/h.CONCLUSION:The established method has the following merits:high extraction rate,fast speed,simple technics,pollution-free,pure extraction etc.

Objective To explore the expression and its significance of growth-associated protein(GAP-43 ) and brain-derived neurotrophic factor (BDNK) receptor TrkB gene in rat hippocampus after epilepsy induced by pilocarpine (PILO). Methods In situ hybrid histochemical method was used to observe the changes of the expression of GAP-43 and TrkB mRNA in hippocampus after status epilepticus( SE) induced by PIOL. Results At 3 - 6h following the onset of status epilepticus(SE), TrkB mRNA expression was dramatically high than control groups in the dentate gyrus granule cell and CA3,CA1 pyramidal cell layers(P

This study aimed at exploring the effects of Guttiferone K (GUTK),a compound isolated from G.yunnanensis,on inhibiting the proliferation of pancreatic cancer cells both in vitro and in vivo.MTT assay was used to detect the inhibitory effects of GUTK on the proliferation of five human pancreatic cancer cell lines.Western blot was adopted to detect the apoptosis-related protein expressions of Caspase-3,poly adenosinediphosphate-ribose polymerase (PARP) and Bcl-xL.For in vivo study,the human pancreatic cancer cell MIA PaCa-2 was orthotopically injected into the pancreatic tail of the orthotopic mice.One week later,GUTK was administered by intraperitoneal (i.p.) injection every other day for 4 weeks.The volume and weight of the tumor tissue were measured.The protein expression level of cleaved caspase-3 in tumor tissue of all the groups was quantified by immunohistochemistry.As a result,it was found that GUTK effectively inhibited the proliferation of the five human pancreatic cancer cell lines at a low concentration.GUTK induced caspase-related apoptosis by triggering a series of events in MIA PaCa-2 cells including cleaved Caspase-3 and PARP activation,Bcl-xL down-regulation,and eventually cell death in a time and dose dependent manner.Furthermore,in vivo study revealed that intraperitoneal injection of GUTK significantly suppressed the growth of pancreatic cancer cells in the orthotopic mouse models,and the protein level of cleaved caspase-3 was increased in the GUTK and gemcitabine treated groups.It was concluded that GUTK induced apoptosis in human pancreatic cancer both in vitro and in vivo,and was potential to develop into a clinical anticancer agent.

Objective To analysis the effect of lens opacity on the measurement of retinal vessel oxygen saturation.Methods This was a cross sectional study.Forty four eyes of 44 patients with different degrees of lens opacity were enrolled.There were 23 males and 21 females.The patients aged from 48 to 84 years,with the mean age of(71.8± 10.3) years.The mean best corrected visual acuity was 0.65±0.22.The mean intraocular pressure was (14.2± 4.3) mmHg (1 mmHg=0.133 kPa).The mean equivalent spherical degree was (-0.05 ± 2.10) D.The opitical quality analysis system was applied to measure intraocular objective scattering index (OSI) caused by lens opacity.According to the OSI,the opacity of lens was divided into four groups.Patients with OSI value ＜ 1.0 was grouped to level 1,which indicated that the lens were basically transparent;patients with OSI value between 1.0 and 3.0 was grouped to level 2,which indicated early cataract;patients with OSI value between 3.0 and 7.0 was grouped to level 3,which indicated progressive cataract;patients with OSI value ＞ 7.0 was grouped to level 4,which indicated the mature stage of cataract.The retinal oximeter Oxymap T1 was used to capture the fundus images under different wavelengths.Pearson correlation analysis was used to analyze the correlation between retinal oxygen saturation and age,intraocular pressure,equivalent spherical degree and OSI.One way ANOVA was used to analyze the difference of retinal oxygen saturation among groups.Results The mean retinal arterial oxygen saturation,venous oxygen saturation and arteriovenous difference was (90.70± 6.46)％,(47.34 ± 13.51)％,(43.36 ± 10.09)％,respectively.The correlations of retinal arterial oxygen saturation,venous oxygen saturation and arteriovenous difference with age,intraocular pressure,equivalent spherical degree was not statistically significant (all P＞0.05).The retinal arterial oxygen saturation and venous oxygen saturation was negatively correlated with OSI (r=-0.462,-0.500;P=0.002,0.001),the arteriovenous difference and OSI was positively correlated (r=0.373,P=0.013).According to lens opacity,there were 11 eyes in level 1,9 eyes in level 2,14 eyes in level 3,10 eyes in level 4.There were significant differences of retinal artery and venous oxygen saturation among different lens opacity levels (F=5.340,4.710;P=0.003,0.007);meanwhile,the arteriovenous difference was not significantly different (F=2.048,P=0.123).The retinal arterial oxygen saturation and venous oxygen saturation was significantly lower in the level 4 lens opacity group than any other three groups (all P＜0.05),but there was no statistically significant difference among level 1 to level 3 lens opacity group.Conclusion The effect of lens opacity of level 1 to level 3 is limited on the measurement of retinal oxygen saturation,but level 4 lens opacity will cause decrease of retinal artery and venous oxygen saturation.

Objective To explore the effect of comprehensive nursing intervention in patients with acute myocardial infarction (AMI).Methods A total of 100 patients with acute myocardial infarction were randomly divided into two groups.The patients in the control group were given routine nursing and the patients in the experimental group were given comprehensive nursing intervention.Angina severity,quality of life,psychological status,incidence of adverse cardiac events and family care satisfaction before and after intervention were compared.Results Compared with the control group,the degree of physical activity limitation,angina pectoris stabilized status,angina pectoris attack status,treatment satisfaction and disease cognition score of the experimental group were significantly higher than that of the control group.After the intervention,the physiological function,physiological function,social function,emotional function,energy,mental health and general health status scores were significantly higher than the control group;Anxiety and depression scores were significantly lower than the control group;The overall incidence of adverse cardiac events was significantly lower (P ＜ 0.01).Conclusion The comprehensive nursing intervention has a significant effect in the first aid of acute myocardial infarction patients,which can improve the satisfaction of the family members.