Objective To explore the change of ECG after chemotherapy and radiotherapy in treatment of patients with breast cancer. Methods 58 patients with breast cancer were treated with surgery,after operation the observation group were treated with chemotherapy and radiotherapy,and the control group was given chemotherapy singly.The change of ECG was compared between two groups. Results In the observation group,11 patients had abnormal ECG(37.93%),In the control group,2 patients had abnormal ECG(9.52%)(x2=8.03,P ＜0.05＝.In addition,in the observation group the incidence of abnormal ECG in the patients with left breast cancer(7/15)was slightly higher than that with right breast cancer(4/14)(x2=1.01,P ＞ 0.05). Conclusion The treatment of chemotherapy combined with radiation could significantly increase cardiac toxicity in the patients with breast cancer.

OBJECTIVE To conduct the centralized management of the medical instrument in the supply room,and to assure the quality and validation of the clinical nursing and the patient safety. METHODS The cleaning and packing procedures of the instrument were standardized.The reusable instrument would be concentrated to clean,pack,and sterilize.To create a photograph management system,and use the photos to distinguish specialized instrument. RESULTS The instrument was reprocessed in the supply room instead of the clinical departments by the method of the centralized management of the instrument,which provided better instrument care and reprocessing. CONCLUSIONS The supply room conducts the centralized management of the medical instrument and enhances the quality of the instrument reprocessing.

The quail model of hyperuricemia combined with abdominal obesity was induced by high purine diet.Body weight in model group showed no change.Serum uric acid level in model group was increased significantly on 7,14,21,and 28 d( P＜0.05 or P＜0.01 ).Abdominal fat index in model group increased significantly on 28d.On 7 d and 28 d,serum free fatty acid level was increased significantly.Acetyl-CoA carboxylase( ACC ) protein expression in the liver of model quail was increased as shown by ELISA and immunohistochemisty ( P＜0.05 or P＜0.01 ),suggesting that the alteration of ACC expression contributes to the pathogenesis of hyperuricemia combined with abdominal obesity.

Objective To explore the effects of interactive health education on the knowledge and behaviors of medicine use and the biochemistry indicators in hemodialysis patients. Methods Forty-eight eligible hemodialysis patients were intervened with interactive health education once every month. After three times'intervention, phosphorus, hemoglobin and hematocrit level were compared before and after the intervention. Results After the intervention, phosphorus, levels of hemoglobin and hematocrit were all significantly improved ( P<0 . 001 ) compared with that before the intervention . Conclusion The interactive health education can improve the knowledge and compliance behaviors in medicine use, serum phosphate and symptoms of anemia in hemodialysis patients.

AIM:To explore the changes in immune function children patients with repeated respiratory infection(RRI)and the influence of nucleotide and casein injection on it.METHODS: 45 patients with RRI were arranged in therapy group and were injected with nucleotide and casein injection.32 patients with acute respiratory infection were arranged in control group who were not injected with nucleotide and casein injection.The effect of treatment, change of IgA,IgG,IgM,T-lymphocyle subgroups and reaction intensity of PHA were observed.RESULTS: There were very significant decline of IgA and IgG level(P

Objective To investigate the diagnostic value of Heparin-binding protein (HBP) in maintenance hemodialysis patients in catheter-related infections.Methods 75 patients with maintenance hemodialysis were enrolled in the study and divided into observation group(n=45,with catheter-related infections) and control group(n=45,without catheter-related infections).The serum HBP,procalcitonin (PCT) and C-reactive protein (CRP) levels were detected.The values in diagnostic of catheter-related infections and Gram-negative bacterial infections were analyzed by receiver operating characteristic(ROC) curve.Results There were 34 cases of patients infected by Gram-positive bacterial with the rate of 75.6% and 11 cases of patients infected by Gram-negative bacterial with rate of 24.4%.The serum HBP,PCT and CRP levels in observation group were significantly higher than those in control group and the serum HBP,PCT levels of patients infected by Gram-negative bacterial were higher than those of patients infected by Gram-positive bacterial (P<0.05).The area under curves of HBP,PCT,CRP in diagnosis of catheter-related infections were 0.955,0.890,0.871 with sensitivity of 86.7%,88.9%,62.2% and specificity of 93.3%,73.3%,93.3%.The value of HBP in diagnosis of Gram-negative bacteria infections was better than PCT and CRP.The area under ROC was 0.818 and the cut off value was HBP>27.52 μg/L with sensitivity of 100.0% and specificity of 61.8%.Conclusion Serum HBP,PCT and CRP may be the effective indicators for diagnosis of catheter-related infections,and the HBP has important reference value for diagnosis of Gram-negative bacterial infections.

To pay attention to functional virtual laboratory,we can make new networks management,open laboratory and develop students' innovative ability.

Objective To explore the effect of clinical nursing pathway(CNP)on allergic rhinitis in patients with subcutaneous immunotherapy.Methods One hundred and twenty patients with allergic rhinitis treated with subcutaneous immunotherapy were equally randomized into the observation group and the control group.The former group were managed with clinical nursing pathway and the latter received only routine nursing care.The curative effect of subcutaneous immunotherapy was compared between the two groups including local adverse reactions,treatment compliance,patients' satisfaction and quality of life.Results The scores by symptom assessment at time points of half a year,years 1,2 and 3 showed the symptoms at different time points between the groups were significantly different by the comparison between time and the interactive effect of main effect(F=678.24,P<0.01),and the symptoms in the two groups were both alleviated with the extension of treatment due to the compared time effect at F=47.16, P<0.01.The scores on the symptoms in the observation group were significantly lower than those of the control group at different time points(P<0.01).The experiment group was better than the control group in terms of local adverse reactions,treatment compliance, quality of life and patient's satisfaction(P<0.01)Conclusion Clinical nursing pathway can improve patient's compliance,improve immunotherapy,reduce adverse reactions and improve the quality of life of patients as well as patients' satisfaction.

Objective To analyze the whole genomic DNA methylation level and estrogen receptor α (ERα) gene promoter methylation status in systemic lupus erythematosus (SLE) with atherosclerosis (AS) in model mouse,and to explore its role in the pathogenesis of SLE with AS.Methods Eleven apoE-/-C57BL/6 mice were randomly divided into the model group (SLE+AS group) and the control group (AS group).Eleven wild C57BL/6 mice were also randomly divided into the model group (SLE group) and the control group (blank group).Single intraperitoneal injection of pristane 0.5 ml for the model group,single intraperitoneal injection of normal saline 0.5 ml for the control group.Eight months after injection,all mice were sacrificed,genomic DNA was extracted from spleen.The total genomic DNA methylation level was detected,and pyrosequencing was performed to determine the methylation status of ERα gene promoter.The differences between groups were compared.ANOVA,LSD-t test,Tamhane's T2 test were used for statistical analysis.Results The total genomic DNA methylation levels were (4.7±1.5)％,(5.1±0.5)％,(6.6±1.6)％,(7.5±1.6)％ respectively in the SLE+AS group,AS group,SLE group,blank group respectively,the average methylation indices of ERα gene promoter were (13.0±3.1)％,(26.7±7.2)％,(15.7±3.8)％ and (21.4±4.2)％ respectively.The total genomic DNA methylation level and the average methylation index of ERα gene promoter in the SLE+AS group and the SLE group was significantly lower than that of the blank group (P＜0.05).Compared with the AS group,the total genomic DNA methylation levels and the average methylation index of ERα gene promoter in the SLE+AS group were significantly decreased (P＜0.05).Conclusion The total genomic DNAs and the ERα gene promoters in SLE with AS model mouse are in low-methylation status.The results of this study suggest that epigenetics may play an important role in the pathogenesis of SLE with AS.

Objective To study the intestinal microflora changes in children with Henoch-Sch?nlein purpura (HSP). Methods The feces of 64 children with HSP (the experimental group) and 25 healthy children (the control group) were collected. 16S rRNA/DNA fluorescent quantitative polymerase chain reaction were performed to detect bacterial content of Lactobacillus, Biifdobacterium and Escherichia coli in feces. Results The amounts of Lactobacillus, and Biifdobacterium in the HSP group were lower than those of the healthy group, and the amounts of E.coli were higher, but the differences were not statistically signiifcant. The amounts of Lactobacillus and Biifdobacterium in the HSP children with gastrointestinal symptoms (abdominal pain, vomiting, e.g.) were signiifcantly lower than those in HSP children without gastrointestinal symptoms and in the healthy group. The difference was signiifcant (P<0.05). Conclusions It suggests that intestinal microlfora in children with HSP in acute phase may exist disorders, especially in the HSP children with gastrointestinal symptoms.