1.INVESTIGATION OF EQUITY PRIMARY HEALTH CARE INSTITUTIONS PAY FOR PER-FORMANCE IN ZHEJIANG
Modern Hospital 2015;(10):114-116,118
Objective To understand the basic medical and health personnel's perception of fairness for pay for performance program .Methods This study used document analysis , expert consultation , pre-survey method to de-sign the questionnaire , and then was conducted on 367 medical and health personnel at the basic level in Zhejiang Prov-ince, analysis of variance , correlation analysis were used for statistical analysis .Results Only 0.13% of the basic medical and health personnel think pay for performance program is very fair;23.16%of people think pay for perform-ance program is fair;29.43%of people do not know whether pay for performance program is fair;34.06%of people think pay for performance program is not fair;11.99% of people think pay for performance program is very unfair . There were no significant differences between age , educational levels, working time, professional qualifications and po-sition(p>0.05).Staff in different areas of the perception of pay -for-performance fairness exists obvious difference (p<0.05).Conclusion Only a small portion of basic medical and health personnel think pay for performance pro -gram is fair, and it may directly affect the enthusiasm of staff , reduce the work quality and efficiency of basic medical and health personnel .It suggests that relevant departments should improve the pay for performance program .
2.Telomerase activity in transitional cell carcinoma after intravesical instillations of Serratia Marcescens
Xiaowen HE ; Jun LI ; Xiaoqing JIN
Chinese Journal of Urology 2001;0(07):-
Objective To study changes of telomerase activity in transitional cell carcinoma after intravesical instillation of Serratia Marcescens . Methods TRAP-PCR and TRAP-sliver staining were used to detect telomerase activity in 20 patients with TCC after intravesical instillation of Serratia Marcescens . Results Positive rate of telomerase activity detection in TCC patients after intravesical instillation of Serratia Marcescens was 50%(10/20),significantly lowed than before the intravesical instillation (90%,18/20), P
3.Detection of K-ras mutation in DNA extracted from the plasma of patients with colorectal cancer
Xiaowen HE ; Jun LI ; Xiaoqing JIN
Chinese Journal of Digestion 2001;0(01):-
Objective The purpose of this study was to determine the value of K-ras mutation in DNA extracted from the plasma as clinical indicator of colorectal cancer. Methods Point mutation at codon 12 of K-ras gene was assayed by polymerase chain reaction of sequence-specific primers in DNA extracted from the plasma and tumors from 32 patients with colorectal cancer. The mutation was further confirmed by dideoxy-mediated chain-termination method of DNA sequencing. Results Fourteen patients (44%) had a codon 12 K-ras mutation within their primary tumors and identical mutations were found in the plasma DNA of 13 patients (93%). Mutant DNA was not detected in the plasma specimens of 18 patients whose tumors tested negative for K-ras alterations or in 5 healthy control subjects. Conclusion Preliminary results suggest the detection of K-ras mutations in circulating DNA extracted from the plasma specimens may have some clinical uti- lity in the detection of colorectal cancer.
4.Effect of Bushenhuoluofang on Follicular Number, Endomelrium Area and Endocrine of Androgen Sterilized Rats
Qian HE ; Yonghong WANG ; Xiaoqing ZHOU
Chinese Journal of Information on Traditional Chinese Medicine 2006;0(08):-
model rats group. Conclusion Bushenhuoluofang increased the weight of ovary and uterus, follicular number and endomelrium area of ASR. By affecting the level of FSH and LH of the rat, improves its endocrine function and the growth of follicle, Bushenhuoluofang achieved the purpose of promoting ovulation.
5.Influence of anesthesia methods on early postoperative cognitive function in elder orthopedic patients
Bin YU ; Kangmei HE ; Xiaoqing ZHANG
The Journal of Clinical Anesthesiology 2010;26(2):110-112
Objective To study the influence of anesthesia methoda on early postoperative cognitive function in elder orthopedics patients. Methods Fifty patients aged more than 65 years old with fractured femur randomly received general anesthesia(group G, 28 cases) or unilateral combined spinal epidural anesthesia(group E,22 cases). The assessment of postoperative cognitive {unction referred to the scores that achieved in neuropsychological testing with mini mental state examination (MMSE) by special psychological person on the day before and the first day after surgery. It was considered to he postoperative cognitive dysfunction (POCD) when the score was ≥1 standard deviation(SD). Results The score of MMSE was lower in group G than that in group E on the first day alter surgery(P<0. 05). The incidence of POCD on the first day after surgery was higher in group G than that in group E(42.9% vs. 13. 6%) (P<0. 05). Conclusion Compared to combined spinal epidural anesthesia, more POCD takes place in the eldedy underwent orthopedic surgery under general anesthesia.
6.Stabilization of plasma homocysteine and other related thiols concentrations in whole blood
Jun HE ; Yanna BAN ; Xiufang TANG ; Xiaoqing ZHANG ; Min DING
Chinese Journal of Laboratory Medicine 2011;34(7):598-602
Objective To investigate the effects of different stabilizers, time and temperature before centrifugation on the stability of homocysteine (Hcy) and other related thiols levels involving EDTA-containing whole blood.Methods Blood was drawn from 17 healthy adults and collected into tubes containing EDTA, EDTA plus NaF and EDTA plus 3-deazaadenosine(3DA),then stored on crush ice(0-4 ℃) immediately or at room temperature(25 ℃).Plasma was separated at 0, 3, 6, 24 and 48 hours, respectively.The levels of plasma total Hcy (tHcy), total cysteine (tCys), tatal cysteinylglycine (tCysGly) and tatal glutathione (tGSH) were measured by HPLC.The plasma immediately separated was used as baseline sample. Results In EDTA tubes stored at room temperature, tHcy levels increased by 38.5%, 64.2%, 141.9%, 225.4% for 3, 6, 24, and 48 h, respectively.The levels of tCysGly and tGSH increased by 20.0% and 37.9% within 3 h, however, tCys decreased by 3.5%.The levels of the thiols increase by less than 5% up to 6 h in EDTA tubes stored on crush ice.In EDTA-3DA and EDTA-NaF tubes, no statistical differences were observed in the plasma levels of tHcy, tCys,tCysGly and tGSH compared with their respective baseline values at room temperature for 3 h(EDTA-3DA tubes:F=0.01,0.94,0.09,0.01,all P>0.05;EDTA-NaF tubes:F=0.85,0.04,0.03,0.02,all P>0.05).Conclusions The EDTA-plasma levels of tHcy and other related thiols are time and temperature-dependent. There is a strong need for standardization of blood sample collection and processing in tHcy and other thiols assays. The plasma concentrations of tHcy, tCys, tCysGly and tGSH were stable for 3 h at least in the EDTA-3DA and EDTA-NaF tubes kept at room temperature.
7.Analysis on standardized training for graduates majored in health management
Baojing XIA ; Hongdian YU ; Jun HE ; Xiaoqing LI ; Shaoping WU
Chinese Journal of Medical Education Research 2012;11(7):732-734
To explore the model of the standardized training for health management talents by referring to the standardized training for resident-doctors.This article analyzed the necessities of the model of the standardized training for health management talents from two aspects.After that,it elaborated on the significance of standardized training.At last,it proposed some ideas about constructing the standardized training for health management talents.
8.Isolation of Endophytic Fungus from Plantago Asiatica L. and Its Microbial Inhibition Activity
Jiangtao BI ; Ping HE ; Xiaoxia WANG ; Jing WANG ; Xiaoqing GUAN
World Science and Technology-Modernization of Traditional Chinese Medicine 2013;(3):520-526
This study was aimed to explore the resource diversity and microbial inhibition activity of endophytic fungi from medicinal plant Plantago asiatica L. The endophytic fungi were isolated from the root, stem and leaf of the host by tissue inoculation culture and five plant pathogenic fungi and four bacteria strains used as indicating microbes to test microbial inhibition activity by agar plate antagonistic action and modified agar gel diffusion methods. The results indicated that thirteen fungal endophytic strains were isolated from the host. Most of them came from stem, then leaf, and root as the least in number. The isolated strains attribute to five genera, two fam-ilies, and two orders based on morphological characteristics. For the isolated strains, eleven of them were found to have some microbial inhibition activities against one or more indicating fungi, making up 84.6% of the total iso-lates. Six isolated strains had some antimicrobial activities against one or more indicating bacteria, amounting to 46.2% of the total isolates. Three isolated active strains, which are PAEFS001, PAEFS007 and PAEFS008, ex-hibited evident inhibition activities against five kinds of pathogenic fungi used in the trials respectively. The strain of PAEFS001 ascribed to Ozonium sp. Both strains of PAEFS007 and PAEFS008 ascribed to Aspergillus sp. One active strain of PAEFS003 showed evident antibacterial activities to Bacillus subtilis and Staphyloccus aureus, which belonged to Fusidium sp. The endophytic fungi from medicinal plant Plantago asiatica L. have evident an-timicrobial activities. Their inhibition activities against pathogenic fungi have relatively broad spectrum. And their inhibition activities to both Bacillus subtilis and Staphyloccus aureus as G+ are evident and have certain selectivi-ty. It is feasible to find new bioactive compounds associated with endophytic fungi from Plantago asiatica L. Fur-ther research and development of the endophyic fungi will be important for the integrated utilization of the host.
9.To compare the role of Gd-EOB-DTPA dynamic contrast-enhanced MRI with multi-detector row computed tomography (MDCT) in patients with focal liver lesions
Xiaoqing LI ; Zhipeng ZHOU ; Songqing HE ; Xiaoping CHEN
Chinese Journal of Hepatobiliary Surgery 2013;19(11):815-820
Objective To compare the role of Gd-EOB-DTPA dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) with multi-detector row computed tomography (MDCT),and to determine the sensitivity,specificity and accuracy in focal hepatic lesions.Methods A retrospective analysis was conducted on 32 patients with focal hepatic lesions who had undergone MRI and MDCT examinations.These patients were divided into two groups:the CT group and the MRI group.The results were analysed using receiver operating characteristic (ROC) curves.Result There were 185 focal hepatic lesions.The sensitivity,specificity and the area under the ROC curve (AUC) were 86.5%,90.9%,0.855,respectively for the MRI group and they were significantly higher than the CT group (63.6%,54.5%,0.532).For detection of lesions <1 cm,the sensitivity,specificity and the area under the ROC curve (AUC) for the MRI group were 90%,86.6%,0.886,respectively,which were also significantly higher than the CT group (50.5%,45.5%,0.500).When combined with pathological findings and follow-up,the diagnostic accuracy was 40.6% using Gd-EOB-DTPA DCE-MRI.Conclusion Gd-EOB-DTPA DCE-MRI has a higher detection rate,better accuracy and diagnostic value for focal liver lesions (<1 cm) than MDCT.
10.Plasma concentrations of homocysteine and other aminothiol in end-stage renal disease patients
Jun HE ; Xiaoqing ZHANG ; Wenjuan WANG ; Min DING
Chinese Journal of Laboratory Medicine 2009;32(3):296-299
Objective To investigate the changes in concentrations of the homocysteine (Hey) and other aminothiols (ESRD) in plasma of end-stage renal disease (ESRD) patients before and after hemodialysis (HD). Methods 26 chronic renal failure patients treated with hemodialysis plus 54 healthy controls were randomly chosen. The concentrations of plasma total homocysteine (they), total cysteine ( tCys), total cysteinylg]ycine (tCysGly), total glutathione (tGSH) were determined by HPLC-fluorescence detection (FD). The concentrations of serum lipids were detected and several renal function tests were conducted. Results The concentrations of they ( 16. 70 ± 3.51 μmol/L vs 10. 95±3.07 μmol/L, t =3. 625,P <0. 01),tCys(341.87±70.65 μmoL/L vs 249.76 ± 13.18 μ mol/L,t =6.219,P <0.01), tCysGly(41.33 ± 32. 95 μmol/L vs 31.3 ± 11.78 μmol/L, t = 3. 530, P <0.01 ) in pre-hemodialysis plasma were significantly elevated , and tGSH ( 5.91 ± 0. 08 μmol/L vs 9. 33 ± 2. 62 μ mol/L, t =-5.404, P < 0. 01 ) was significantly decreased compared with the control group. The concentrations of tHey and tCys (11.74 ± 3.42 μmol/L and 272. 67 ± 64. 18 μmol/L) in post-hemodialyais plasma were significantly decreased compared with in pre-hemodiaIysis plasma, but they could not be restored to normal levels. However, the concentrations of tCysGly(41.33 ± 32. 95 μmol/L vs 44. 93 ± 13.88 μmol/L,t =-0.758, P>0.05) and tGSH (5.91±0.08 μmol/L vs 5.93±0.38 μmol/L,t = -0.068,P >0.05) in pre-hemodialysis plasma and post-hemodialysis plasma didn't change significantly. There were significant positive correlations between plasma levels of they and tCys(r =0. 458 2 ,P <0. 01 ). There was significant negative correlations between plasma levels of tHcy and tGSH ( r =-0.609 9, P=0.000 9). Nevertheless, tHcy levels were was not correlated with tCysGly levels and other serum lipid parameters. Conclusion There is a high prevalence of metabolic disturbance in Hcy and other related aminothiols in ESRD patients.