1.Effect of calcium-sensing receptor on intracellular calcium, cell proliferation and migration of SGC-7901 cell line
Jian SUN ; Xiaoqin LIU ; Qi WU ; Li LI ; Hongtao ZHAO ; Yankun HAO ; Zhifang LANG ; Hairong LUAN
Chinese Journal of Clinical and Experimental Pathology 2015;(10):1140-1144
Purpose To observe the functional expression of calcium sensing receptor ( CaSR) in human gastric cancer SGC-7901 cell line, the effect of CaSR on intracellular calcium, cell proliferation and migration of SCG-7901. Methods The expression and distribu-tion of CaSR were detected by Western blotting and immunofluorescence observation in SGC-7901. The intracellular concentration of free calcium ( [ Ca2+] i ) was determined by confocal laser scanning microscopy. MTT, flow cytometry and scratch test were used to an-alyze the impact of CaSR the proliferation and the migration capabilities of SGC-7901 cell. Results CaSR protein was expressed in SGC-7901. Extracellular calcium or calindol significantly increased the expression of [Ca2+]i, CaSR and E-cadherin;In addition, the migration capabilities were decreased. Conclusion CaSR is expressed in SGC-7901. The activation of CaSR induces the expression of E-cadherin, and decreases migration ability.
2.An equation for calculating the osmolarity of adult total nutrient admixture
Xiaoqin LANG ; Boyu LU ; Xue BAI ; Jiaqiang XU ; Bin ZHAO ; Dan MEI
Chinese Journal of Clinical Nutrition 2020;28(2):87-92
Objective:To establish a predictive equation for the osmolarity of parenteral nutritional prescription in China.Methods:From July 2019 to September 2019, 2 480 individualized samples of 328 different parenteral nutritional prescriptions (adult) of Peking Union Medical College Hospital were collected, and the osmolarity of parenteral nutritional solution samples was determined by a freezing point reduction method. Pearson χ2 test and a multiple linear regression analysis were utilized to establish a prediction equation for the osmolality of parenteral nutritional solution. Results:The average osmolarity of parenteral nutritional prescription was (1 164.20 ± 252.59) mOsm/kg, and the best fitting equation was (9.66A+ 7.88G+ 3.52F+ 36.4Na+ 27.55K+ 3.38P+ 7.46W-250)/V.Conclusion:The osmolarity is determined accurately and effectively by the fitting equation, which provide a benefit reference for the formulation, review and selection of clinical parenteral nutrition prescription especially in China.
3. Combined anluohuaxianwan and entecavir treatment significantly improve the improvement rate of liver fibrosis in patients with chronic hepatitis B virus infection
Liang MIAO ; Wanna YANG ; Xiaoqin DONG ; Zhanqing ZHANG ; Shibin XIE ; Dazhi ZHANG ; Xuqing ZHANG ; Jun CHENG ; Guo ZHANG ; Weifeng ZHAO ; Qing XIE ; Yingxia LIU ; Anlin MA ; Jun LI ; Jia SHANG ; Lang BAI ; Lihua CAO ; Zhiqiang ZOU ; Jiabin LI ; Fudong LYU ; Hui LIU ; Zhijin WANG ; Mingxiang ZHANG ; Liming CHEN ; Weifeng LIANG ; Hui GAO ; Hui ZHUANG ; Hong ZHAO ; Guiqiang WANG
Chinese Journal of Hepatology 2019;27(7):521-526
Objective:
To explore the improvement rate of liver fibrosis in patients with chronic hepatitis B virus infection who received entecavir alone or in combination with anluohuaxianwan for 78 weeks.
Methods:
Patients with chronic HBV infection were randomly treated with entecavir alone or in combination with anluohuaxian for 78 weeks. Ishak fibrosis score was used for blind interpretation of liver biopsy specimens. The improvement in liver fibrosis condition before and after the treatment was compared. Student's t test and non-parametric test (Mann-Whitney U-Test and Kruskal-Wallis test) were used to analyze the measurement data. The categorical variables were analyzed by Chi-square test method and Spearman’s rank correlation coefficient was used to test bivariate associations.
Results:
Liver fibrosis improvement rate after 78 weeks of treatment was 36.53% (80/219) and the progression rate was 23.29% (51/219). The improvement of liver fibrosis was associated to the degree of baseline fibrosis and treatment methods (