1.Effect of thymosin α1 on immunologic function and cytokines in Patients with severe infection
Xiaoqiang HAN ; Yunxia LEI ; Keqiang SHI
International Journal of Surgery 2008;35(7):462-465
Objective To investigate the effect of thymosin α1 on immun ologic function and cytokines in the abdominal sepsis patients.and naddiscuss the therapeutic function of thymoin α1 in sepsis.Methods Thirty-eight sepsis patients admitted between May 2006 and May 2007 in the Hospital of Weifang Medical University.Patients were divided into the treatment group(20 cases)and control group(18 cases)randomly.Thymosin α1 was used in the treatment group on the basis of infection controlled.The concentration of cytokines(TNF-α,IL-6,IL-10)and the percent age of CD3,CD4,NK cell were observed.Results The concentrations of TNF-α and IL-6 were increased significantly in bothgoups.But it was more prominently in the control group.The concentration of IL-10 and the percent age of CD3,CD4,NK cell increased more significantly in thymosinod treatment group than those in the control group.Conclusions Thymosincd canmodulate the release of TNF-α,IL-6,IL-10 and mitigate the inflammatory reaction,which may improve the immunologic function.
2.Safety and effectiveness of modular flexible ureteroscope combined with holmium laser lithotripsy in treatment of bilateral renal calculi smaller than 1.5 cm
Zhenyu CUI ; Wenzeng YANG ; Hongyue ZHOU ; Feng AN ; Xiaoqiang SHI
China Journal of Endoscopy 2017;23(6):102-104
Objective To evaluate the safety and effectiveness of modular flexible ureteroscope combined with holmium laser lithotripsy in treatment of bilateral renal calculi smaller than 1.5 cm. Methods Clinical data of 24 patients from August 2013 to March 2016 using a modular flexible ureteroscope in treatment of bilateral renal calculi smaller than 1.5 cm was retrospectively analyzed. The clinical data included operation time, stone clearance rate and occurrence of complications. Results All the 24 patients were successfully placed in bilateral ureteral sheath soft lenses, and enter lithotripsy smoothly. The operation time was 40~105 (71.0 ± 21.5) min. Lithotripsy success rate was 100.00% and 1 month stone clearance rate was 89.50% (43/48), 5 sides with residual stones diameter 7~10 mm, were given extracorporeal shock wave lithotripsy, 3 months stone clearance rate was 93.75% (45/48), 3 sides with residual stones 5~7 mm located lower calyx accepted regular review. 4 cases with postoperative fever were cured after anti-inflammatory treatment. There was no bleeding, ureteral perforation, postoperative avulsion, renal dysfunction, septic shock and other complications. Conclusion Modular flexible ureteroscope lithotripsy in treatment of bilateral renal calculi smaller than 1.5 cm is safe and effective.
3.Construction of shSet7/9 vector and its function in HepG2
Kejun MA ; Xingchen SHI ; Ping LI ; Xiaoqiang LI ; Wen REN ; Long QIN ; Xinhe SHI
Journal of Xi'an Jiaotong University(Medical Sciences) 2015;(6):753-757
Objective To silence human gene Set7/9 and screen out stable transfection cell line in hepatocellular carcinoma cell line HepG2 so as to investigate the impact of down-regulation of Set7/9 in cell line HepG2 and provide experimental foundation for studies on the effect of set7/9 in HepG2.Methods The target oligo was designed and synthesized;shRNA interference vector and the control vector were constructed and transfected into HepG2 cells;the stable transfection cells were screened out.Then Real-time PCR and Western blot were performed to detect the silence of Set7/9 according to both gene expression and protein expression level. Results The shRNA interference vector was constructed and transfected into HepG2 cells successfully.Compared with that in the negative control group,the expression of Set7/9 was dramatically downregulated (P < 0.05 ). Meanwhile,the expression of related protein Sirt1 and Suv39h1 was upregulated 8.4 folds and 1.1 fold, respectively.Conclusion Downregulation of Set7/9 expression can upregulate Sirt1 and Suv39h1,suggesting that Set7/9 may affect the activity of HepG2 cell lines.
4.Study on correlation between MR spectroscopy and whole pathologic seetion in benign prostatic hyperplasia
Zhijie SHI ; Feng LI ; Shouhong WANG ; Xiaoying WANG ; Lijun ZHAO ; Xiaoqiang LI
Chinese Journal of Radiology 2008;42(9):954-956
Objective To investigate the metabolic characteristics,clinic values of different tissues in benign prostatic hyperplasia by correlating MR spectroscopy(MRS)with whole pathologic sections.Methods MRS presentation was studied in 16 patients with benign prostatic hyperphsia.The peak values of citrate,choline and creatine were measured,and the mtios of(choline+creatine)/citrate(cc/c)were obtained.After suprapubic transvesical prostatectomy,the specimens of whole pathologic sections were observed.The values of cc/c of different pathologic types were compared statistically with One.way-ANOVA and t test. Results MRS results were obtained in 578 units,which included 233 glandular tissue units.130 matrical tissue units.165 mixed tissue units and 50 tissue units around urethra.The ratios of (choline+creatine)/citrate of glandular,matrical.mixed tissues and units around urethra were 0.59±0.29.1.23±0.38.0.80±0.27 and 0.68±0.33(F=3.49.P<0.05).The t tests were carried out between every two tissues.The t valUes between glandular tissues and matrical.mixed tissues were 19.60 and 7.09.P<0.05:the t values between matrical tissues and mixed tissues.units around urethra were 13.22 and 9.42,P<0.05;the t valUes between mixed tissues and units around urethra was 9.42,P<0.05.Condusions,The ratios of cc/c ofthree prostate tissues were different in MRS.The metabolic characteristic of the units around urethra Was near to glandular units.MRS may be valuable to the distinctive diagnoses of prostate diseases.
5.Keyhole craniotomy for cholesteatmas in the cerebellopontine angle region presenting as trigeminal neural-gia
Xiaoqiang WANG ; Xinding ZHANG ; Yanming HAN ; Xuefeng SHI ; Zhenbo LAN ; Guokuo HOU ; Niandong CHENG ; Yawen PAN
Chinese Journal of Nervous and Mental Diseases 2016;42(12):705-709
Objective To investigate the clinical utility of keyhole craniotomy for Cholesteatmas in the cerebello-pontine angle region presenting as Trigeminal neuralgia by using keyhole craniotomy. Methods Clinical data of 35 pa-tients with Cholesteatmas in the cerebellopontine angle region presenting as Trigeminal neuralgia was analyzed retrospec-tively. All patients received suboccipital retrosigmoid keyhole craniotomy. Thirty cases who received conventional craniot-omy group were served as control group. A comparative analysis was conducted to assess the difference between these two groups in microsurgical methods, effects and complications. Results All patients were confirmed with MR diagnosis. In keyhole group, all patients had no pain symptoms of trigeminal neuralgia after surgical operation. The symptoms disap-peared immediately in 33 cases and gradually disappeared in 2 cases within one month following keyhole craniotomy. The tumors were totally removed in 25 cases and almost completely in 10 cases. The surgical duration was shorten in key-hole group than in conventional group (126±48 vs. 216±66 min;t=2.536, P<0.05). The amount of bleeding was smaller in keyhole group than in conventional group (91.3±52.2 vs. 186.3±65.4 mL;t=2.163, P<0.05). Postoperative neurologi-cal function was assessed in House-Brackmann. In keyhole group, there were 32 cases in class Ⅰ and 3 in grade Ⅱ. Two patients had hearing loss which was improved gradually in three months. In conventional group, there were 25 cases in class Ⅰ and 5 in grade Ⅱ (χ2=4.158, P<0.05). Postoperative hearing evaluation (AAO-HNS) revealed that there were 33 cases in grade A , and 2 in grade B in the keyhole group, whereas there were 27 cases in grade A and 3 in grade B in the conventional group(χ2=5.167, P<0.05). There were no relapse of tumors and death during 3 month to 5 years follow-up. Conclusions The suboccipital retrosigmoid keyhole craniotomy is a valid choice for Cholesteatmas in the cerebellopontine angle region presenting as Trigeminal neuralgia.
6.Multivariate Analysis on TCM Syndromes of Gastric Cancer
Dazhi SUN ; Lijuan XIU ; Jun SHI ; Jianpeng JIAO ; Long LIU ; Xiaoqiang YUE
Chinese Journal of Information on Traditional Chinese Medicine 2016;23(10):16-20
Objective To explore the composition and distribution law of TCM syndromes in gastric cancer. Methods Based on the multicenter and large-sample clinical epidemiological investigation, the four methods of diagnosis of and clinical materials of 767 cases of gastric cancer were collected, and the database of TCM syndromes in gastric cancer was establish. Factor analysis and clustering analysis were used to explore composition and distribution law of TCM syndromes in gastric cancer. Results Gastric cancer symptoms mainly included fatigue, weight loss, dizziness and other non-specific systemic manifestation, and epigastria discomfort, belching, fullness or eating just a little swelling, pain, acid regurgitation, loss of appetite and other local manifestations. At the same time, the red tongue, moss greasy, pulse fine or string and other traditional Chinese medicine signs were also included. Eliminating 92 cases with too little symptoms, 675 cases were under multivariate analyzed. 25 syndrome variables were selected after initial factor analysis, again through factor analysis 10 factors with eigenvalues more than 1.0 were obtained and the cumulative contribution rate was 60.5%. Through further K-means clustering analysis on 10 common factor integrals, it was found that when all the cases were clustered into 7 classes consistent with clinical practice most. The numbers of patients with the 1-7 type were 165, 82, 90, 79, 88, 95 and 76, respectively. Analysis on the main factors in the combination of professional knowledge, the 7 types were named as the syndrome of spleen and stomach qi stagnation (24.44%), the syndrome of qi and blood deficiency (12.15%), the syndrome of spleen deficiency (13.33%), the syndrome of blood stasis (11.70%), the syndrome of phlegm dampness (13.04%), the syndrome of deficiency cold of spleen and stomach (14.07%), the syndrome of incoordination between liver/gallbladder and stomach (11.41%) respectively. Conclusion The results of multivariate analysis suggests that the location of gastric cancer is in the stomach, and closely related to spleen, liver and gallbladder. The general pathogenesis is asthenia in origin and asthenia in superficiality. The deficiency lies in qi, blood and yang qi, while asthenia superficiality owes to stagnation of qi, phlegm and blood stasis.
7.Methodology Comparison Study on Two Analysis Systems of Procalcitonin
Chenyu WANG ; Liping YANG ; Lei CHEN ; Jing YANG ; Fugang LI ; Xiaoqiang SHI
Journal of Modern Laboratory Medicine 2017;32(4):143-144,147
Objective To evaluate the comparability of the test results of two immunoassay systems based on the electrochemical luminescence and the fluorescence lateral flow immunoassay for serum procalcitonin (PCT).Methods Roche cobas system was used as the reference system,and fluorescence lateral flow immunoassay system of Shanghai Upper biotech company was used as evaluated system.A total of 141 clinical samples during November,2015 were detected by the two systems to obtain the correlation coefficient and the Kappa values at the two cutoff values(0.5,2.0 ng/ml).Results The two systems showed high correlation (Y=1.008X-0.032,r=0.995,P<0.001) and low deviation (t=-0.230,P=0.819>0.05) without statistic significance between two methods.Kappa values were 0.944,0.943 respectively at the two cutoff values (0.5,2.0 ng/ml).Conclusion The test results showed no significant difference between the two immunoassay systems,suggesting a consistency between them for clinical detection of PCT.All the observed indicators reached the clinical diagnostic requirements and the method of quantitative detection of PCT by fluorescence lateral flow immunoassya can be applied for the quick detection of clinical human PCT.
8.IdentificationofCTtargetreconstructioninpersistentinvasivepureground-glassnodules
Dai SHI ; Hongya XIE ; Xiaoqiang LIU ; Wu CAI ; Guangqiang CHEN ; Guohua FAN
Journal of Practical Radiology 2019;35(4):561-564
Objective ToexplorethevalueofCTtargetreconstructionforpureground-glassnodules(pGGN)onidentifyingthe invasivenessofthelungadenocarcinoma.Methods ThepGGNs weredividedintopre-invasivegroup[atypicaladenomatoushyperplasia (AAH),andadenocarcinomainsitu(AIS)]andinvasivegroup[minimallyinvasiveadenocarcinoma(MIA),andinvasiveadenocarcinomas(IA)] accordingtothepathologicresults.ThemorphologicfeaturesofpGGNonCTincludedthelargestdiameters,CTvalue,pleuralindentation,air bronchogram,bubblelucency,vesselconvergence,vesseldilatation,lobulationandspeculation.Twodiagnosticiansevaluatedthemorphologic featuresofpGGNonCT.Binary L o g istic regressionwasusedtoassesstheassociationbetweenCTfindingsandhistopathological classification.ROCcurveanalysiswasusedindiameterandCTvalue.Results Betweenpre-invasiveandinvasivegroup,therewere significantdifferencesindiameter,CTvalue,spiculationandvesseldilatation(P<0.05).Nodifferencewasfoundinlobulated-margin,bubble lucency,airbronchogram,vascularconvergenceorpleuralindentationbetweenthetwogroups(P>0.05).Thediagnosticthresholds forpredictingpGGOinfiltrationwere8.75mminmaximumdiameterand-605HUinCTvaluerespectively.Conclusion ThepGGNwitha diametermorethan87.5mm,theCTvaluemorethan-605HU,andpresencesofspiculationandvesseldilatationsuggeststhatpGGOisinvasive.
9.Research progress on active mechanism and structure feature of polysaccharides from Zizyphus jujube in Rhamnaceae plants
Xiaoqiang DONG ; Chang WEN ; Jindan XU ; Lexue SHI ; Yulong HU ; Jieming LI ; Chunhong DONG ; Kan DING
Journal of China Pharmaceutical University 2024;55(4):443-453
Abstract: The genus jujube (Ziziphus jujuba Mill.) within the Rhamnaceae family encompasses numerous varieties, such as Ziziphus jujuba Mill. var. jujuba, Ziziphus jujuba var. inermis, and var. spinosa, etc. Among these, the jujube fructus has the most abundant cultivated variants across the country, including Ziziphus jujuba cv. Hamidazao and Ziziphus jujuba cv. Huanghetanzao. Jujube plants are rich in variety and are used for both medicinal and food purposes. Polysaccharides, one of the main active ingredients of jujube, are important medicinal components that contribute to its efficacy. Jujube polysaccharides have been found to promote hematopoiesis, exhibit antioxidant and anti-tumor activities, repair liver damage, regulate the immune system, and provide anti-inflammatory effects. By comprehensively summarizing and analyzing the literature on jujube polysaccharides from different varieties and origins, this paper reviews the potential mechanisms of action of jujube polysaccharides in exerting biological activities. It also summarizes the primary structural features, such as relative molecular mass, monosaccharide composition, glycosidic linkage, and the substituent modifications of jujube polysaccharides by sulfation, phosphorylation, carboxymethylation, selenization, and acetylation. This review aims to provide a reference for the research and development of jujube in the fields of innovative polysaccharide drugs and functional foods.
10. Analysis of distribution and drug resistance of pathogens isolated from 159 patients with catheter-related bloodstream infection in burn intensive care unit
Xiaoqiang LUO ; Yali GONG ; Cheng ZHANG ; Meixi LIU ; Yunlong SHI ; Yizhi PENG ; Ning LI
Chinese Journal of Burns 2020;36(1):24-31
Objective:
To analyze the distribution and drug resistance of pathogens isolated from patients with catheter-related bloodstream infection (CRBSI) in burn intensive care unit (BICU).
Methods:
From January 2011 to December 2018, among 2 264 patients who were peripherally inserted central venous catheter at the BICU of the First Affiliated Hospital of Army Medical University (the third Military Medical University), hereinafter referred to as the author′s unit, 159 patients were diagnosed CRBSI, including 131 males and 28 females, aged 43 (1, 79) years. The pathogens primarily isolated from peripheral venous blood and central venous catheter blood/anterior central venous catheter specimen of patients with CRBSI were retrospectively analyzed. API bacteria identification kits and automatic microorganism identification instrument were used to identify pathogens. Broth micro-dilution method or Kirby-Bauer paper disk diffusion method was used to detect the drug resistance of the pathogens to 5 antifungal drugs including fluconazole and itraconazole, etc., and 37 antibacterial drugs including tigecycline and imipenem, etc. Modified Hodge test was used to further identify imipenem- and meropenem-resistant