Objective To investigate the relationship between single nucleotide polymorphism (SNP) at position + 10225 in adiponectin receptor 1 gene and type 2 diabetes.Methods The genotypes of + 10225C/G of adiponectin receptor 1 were detected by polymerase chain reaction with sequence specific primers (PCR-SSP) in 200 type 2 diabetes and 100 health controls.Fasting blood samples of all cases were obtained to extract DNA and detect genotype.Statistical software spss 13.0 was used to analyzed.Results Frequency of G-type allele in type 2 diabetes was 40.5％,that in normal controls was 23.5％,there was significant diffierences in the genotype frequencies of SNP+10225 between type 2 diabetes and health controls (x2 =128.0,P＜0.01).Conclusion AdipoR1 + 10225C/G polymorphism was probably associated with type 2 diabetes,G-type allele might be a genetic risk factor of type 2 diabetes.

Some female spinal cord injured patients may present a raise of serum prolactin which is irrelevant with pregnancy, and re-sults in irregular menstruation and galactorrhea. Hyperprolactinemia may even happen in men after spinal cord injury. This kind of hyperpro-lactinemia does not relate with the level or severity of spinal cord injury, and maintains for various time. The hypersensitivity to thyrotro-pin-releasing hormone may relate to the elevatory serum prolactin and amenorrhea. In addition, there are some suspicious factors, such as chest wall injury, pituitary body contusion, enkephalins increasing, the stimulation of spinal neural pathways, stress, and so on. Bromocripti-ne has been tried for it.

AIM: To establish the quality control standard for Chuanbaizhiyang Lotion(Radix Sophorae Flavescentis, Cacumen Tamaricis, Fructus Cuidii, etc.). METHODS: Radix Sophorae Flavesentis, Fructus Cnidii, Folium Artemisiae Argyi were identified by TLC. The content of osthole was determined by HPLC. RESULTS: The TLC sports developed was fairly clear. The HPLC showed good repeatability. The average recovery of osthole was 96.72% with RSD of 1.04%. CONCLUSION: The method is simple, accurate and can effectively control the quality of Chuanbaizhiyang Lotion.

OBJECTIVEThe purpose of this study is to analyze the incidence and the type of the bifid mandibular canal (BMC) by using cone beam computed tomography (CBCT) images.

METHODSA total of 216 patients (104 female and 112 male) who underwent CBCT were included in this study. The CBCT images were evaluated for the incidence and the type of the BMC.

RESULTSBMC was observed in 39 (18.06%) of 216 patients and 50 (11.57%) of 432 sides, female 18 (17.31 %) and male 21 (18.75%). BMC were classified into four types: type I 17 sides (3.94%), type II 11 sides (2.55%), type III 20 sides (4.63%), and type IV 2 sides (0.46%).

CONCLUSIONBMC is detected at a high rate by using CBCT. When doctors perform surgical procedures in the mandible, they should pay attention to the anatomical variations of BMC of the mandibular canals.

Objective:To determine the shear bond strength and analyze the bonding mechanism of home-made and imported nickel-based alloys to resin-veneers.Methods: Home-made and imported alloy disks were cast by lost-wax technique and embedded in acrylic resin fabricating cylinders.Sixteen specimens of each alloy were divided into 2 subgroups: thermocycling group and non-thermocycling group.Specimens in thermocycling groups were submitted to thermocycling(300 cycles) and those in non-thermocycling groups were just stored in water at 37?C for 24 h(0 cycles).Then the shear bond strength values were determined by universal testing machine.The highest shear bond strength values were recorded and metallurgical analysis was performed on the interface between alloys and resin-veneers.Results: The shear bond strength values of both alloys to resin-veneers were relatively high either before or after thermocycling.The shear bond strength values of home-made alloy+thermocycling group,imported alloy+thermocycling group,home-made alloy+non-thermocycling group and imported alloy + non-thermocycling group were(10.50?2.72),(13.12?2.82),(15.51?3.04) and(18.44?3.15) MPa,respectively.The bond strength was significantly different before and after thermocycling for both alloys(P

Objective To establish a method for the determination of silybin in Fufang Yiganling tablet. Methods RP-HPLC was used and the mobile phase was consisted of methanol-water-glacial acetic acid (48∶52∶1). The detection wavelength was 286 nm. Results The calibration curve of Silybin was linear in the range of 0.238 0～2.380 ?g, linear equation:Y=2.146?106X +1.818?103, r=0.999 9, with average recovery of 98.17% (RSD=1.3%). Conclusions The method is simple, accurate and with good reproducibility. It can be used to control the quality of silybin in Fufang Yiganling tablet.

Objective To establish a method for the determination of Alkaloid in cut crude drug of aconite root.Method Contents of aconitine,hypaconitine and mesaconitine incrude drug of aconite root were determined by LC-MS.It was carried out on Inertsil ODS-3 column(150 mm?4.6 mm,5 ?m),with acetonitrile-ammonium acetate buffer solution(60:40) as mobile phase,at a flow rate of 0.2 mL/min and temperature of 30 ℃.Result The method of determining Alkaloid content in crude drug of aconite root was established and 25 lots of crude drugs were determined.Conclusion The method was sensitive and specifical,can be used to control the quality of cut crude drug from aconite root.

Objective To improve the quality standard for Yangxue Anshen Pills. Method TLC was used to identify caulis spatholobi and herba ecliptae, and HPLC to determine the content of emodin. Results The TLC spots of caulis spatholobi and herba ecliptae were clear. The linear range of emodin was 0.009 92～ 0.124 0 ?g. The average recovery rate was 96.2%, and RSD was 1.1%. Conclusion The methods are simple and accurate, with good reproducity.

Objective To study the quality standard of Anshen Jiannao Mixture. Methods Radix ophiopgonis, Radix et Rhizoma Salviae Miltiorrhizae, Fructus Lycii and Radix et Rhizoma Ginseng were identified by TLC. HPLC was used to determine the content of Schizandrol A on C18 (250 mm?4.6 mm, 5 ?m) ODS column. The mobile phase was consisted of methanol-water (55∶45). The flow rate was 1.0 mL/min. The detection wavelength was at 250 nm. Results The characteristic spot of Radix et Rhizoma Salviae Miltiorrhizae, Fructus Lycii and Radix et Rhizoma Ginseng could be easily examined by TLC. The calibration curve of Schizandrol A was linear in the range of 0.121 8～1.218 ?g, r=0.999 9. The precision RSD was 0.22% (n=6). The reproducibility RSD was 1.1% (n=6). The average recovery rate of Schizandrol A was 97.34% (RSD=1.7%, n =6). Conclusions The method is easy, sensitive and specific with good reproducibility. It can be used to control the quality of Anshen Jiannao Mixture.

Objective To supply safe mechanic refererence for reduction of thoracolumbar burstfracture with pediclc screws and lay biomechanic foundation for developing a new type of digitizing pediclc screws. Methods The change of stress and its scope of pediclc screws during reduction of thoracolumbar burstfracture were tested to determine the relationship of the degree of reduction with stress and strain. Results A total of 15 burstfracture specimens were obtained successfully. Stress was small and changed mildly at the beginning of reduction stage. Stress presented transparent elevation tendency at the stage of body height of 75% of intact until at the stage of 90%. When the height of vertebral body restored from 90% to 95%, the curve of stress changed gently. The maximum stress of left screw was 98.4?10.3 and the right was 101.2?13.4. The stress of bilateral screws did not differ significantly. It was calculated that the left strain was (34.64?3.84)N and the right one was (35.544?3.61)N, the moment was (5.20?0.58)Nm of the left and (5.33?0.54)Nm of the right. There was not significant difference between the strain and the moment of bilateral screws. Conclusion The safe range of reduction force is between 4.5Nm and 6Nm. The burstfracture of vertebral body can be both reduced successfully and prevented from overpropping.