Objective : To investigate the spatiotemporal clustering characteristics of hand, foot, and mouth disease in Shangcheng District, Hangzhou City in 2021, so as to provide insights into prevention and control of hand, foot, and mouth disease. Methods: Data pertaining to the incidence of hand, foot and mouth disease in Shangcheng District in 2021 were collected from Chinese Disease Prevention and Control Information System, spatial and temporal distribution of hand, foot and mouth disease were described, and the spatiotemporal clusters were identified. Results: A total of 2 473 cases with hand, foot and mouth disease were reported in Shangcheng District in 2021, with a reported incidence rate of 186.86/105. The incidence of hand, foot and mouth disease appeared a bimodal distribution, with the first peak seen during the period between May and July (1 164 cases, 47.07%) and the second peak during the period between October and December (659 cases, 26.65%). Hand, foot and mouth disease was reported across all streets in Shangcheng District, with the highest incidence seen in Dinglan Street (398.84/105). There were one primary cluster and ten secondary clusters of hand, foot and mouth disease, and the primary cluster was centered in Jianqiao Street with 1.81 km in radius, which covered 9 communities in Jianqiao Street and was clustered during the period from March 19 and April 13. Of the 10 secondary clusters, there were three clusters in Dinglan Street, one in each of Jiubao, Sijiqing, Wangjiang and Kaixuan streets, and three clusters crossing streets. In addition, no spatiotemporal clusters of hand, foot and mouth disease were identified in Nanxing Street, Qingbo Street or Ziyang Street. Conclusion There was a remarkable spatiotemporal cluster in the incidence of hand, foot and mouth disease in Shangcheng District in 2021, with the peaks in incidence from May to July and from October to December, and the incidence of hand, foot and mouth disease was mainly clustered in the urban-rural junction and the central urban regions.

Sphingosine-1-phosphate (S1 P) is a kind of bioactive messenger which is catalyzed by sphingosine kinases (SphK) and mediates various biological behaviors through binding to sphingosine-1-phosphate receptors(S1PR).Researches demonstrate that the aberration of SphK,S1P and S1PR is closely related to the occurrence and development of cancers,such as leukemia,breast cancer,colorectal cancer and lymphoma.The interaction between S1PR1 and signal transducer and activator of transcription 3 (STAT3) is shown to take part in the pathogenesis of malignant tumors as well.Therefore,they are considered to have the potential tumor-treatment value.

OBJECTIVE:To provide reference for the implementation of Quality Management Guidance of TCM Trust-agent Decocting in Medical Institutions in Shanghai. METHODS:Stratified random sampling method was adopted for the questionnaire of TCM trust-agent decocting in 13 medical and health institutions in the suburb of Shanghai. RESULTS:A total of 13 question-naires were sent out and 13 valid questionnaires were received with the effective rate of 100%. In the 13 medical and health institu-tions,12 medical and health institutions partially or fully adopted trust-agent decocting,and the trust-agent decocting in the 12 med-ical and health institutions accounted for 92.3% in all trust-agent decocting;the 12 medical and health institutions with TCM trust-agent decocting service for patients included 9 pharmaceutical factories and 3 pharmacies;label related problems (including name error,oral and topical label error and stickers unclear) and leakage were the main quality problems,accounting for 33.0%and 31.0%,respectively;83.3% of the medical and health institutions didn’t establish the file of the quality control for TCM trust-agent decocting;decocting process was the most important factor that affects the quality. CONCLUSIONS:Implementing a re-gional Quality Management Guidance of TCM Trust-agent Decocting in Medical Institutions in Shanghai and establishing a system that drug administration,industry associations and medical and health institutions are participated in have great significance to pro-mote the healthy development of TCM trust-agent decocting service and ensure patients’medication safety.

The adenosine triphosphate (ATP)-binding cassette transporter G1 (ABCG1),a member of the superfamily of ATP-binding cassette transporters,is involved in the efflux of cholesterol and phospholipids from cells,and it maintains the intracellular hpid homeostasis.ABCG1 deficiency results in foam cell formation,endothelial dysfunction,and inflammatory reaction,and it further leads to the development and progression of atherosclerosis.However,the role of ABCG1 in atherosclerosis in animal experiments and human studies is still a debatable matter.In this paper,the recent findings on the role of ABCG1 in atherosclerotic disease are reviewed.

Objective To investigate the effects and possible mechanism of action of inhibiting hepatitis B virus X protein (HBx) expression on liver cancer metastasis. Methods The suppression of HBx expression in MHCC97H cells was performed by siRNA interference technique, and the effects of HBx suppression on the metastasis of MHCC97H cells were detected by Matrigel invasion assays and in a lung-metastasis mouse model. The expression levels of related epithelial-mesenchymal transition (EMT) and apoptosis proteins were examined by Western blotting. Results Introduction of HBx-siRNA into MHCC97H cells inhibited the expression of HBx and the ability to metastasize,downregulated the expression of Twist and N-cadherin, and upregulated E-cadherin expression. These changes resulted in inhibiting EMT of MHCC97H cells. Meanwhile, apoptosis involved in the Twist-P53 pathway was also found. Conclusions Inhibiting expression of HBx can decrease the metastatic a-bility of MHCC97H cells by changing EMT and inducing apoptosis.

Objective:To investigate the in vitro effects of platelet-rich plasma(PRP) on human periodontal ligament fibroblasts(PDLFs). Methods: Various concentrations of PRP (10, 50, 100, 200, 300, 500 ml/L) were applied to primary cultures of human PDLFs. MTT assays were utilized to assess cell proliferation ability. Migration was determined by assessing the cell response to a concentration gradient with Transwell chamber. Differentiation was assessed using alkaline phosphatase (ALP) kit. Results: A beneficial effect on proliferation was observed, especially in response to 200 ml/L PRP.PRP had stimulatory effects on the migration of human PDLFs. PRP facilitated differentiation of PDLFs. Conclusion: PRP can exert a positive effect on human PDLFs,but this effect is concentration specific, while higher concentrations is not necessary to result in optimal outcomes.

Objective To study the phenylpropanoid constituents from the roots of Euphorbia hylonoma Hand-Mazz. Methods The chemical constituents were isolated and purified by silica gel and Sephadex LH-20 column chromatography,and the structures were elucidated on the basis of chemical properties and spectral data. Results Three phenylpropanoid constituents were isolated from the acetone extracts of the roots of Euphorbia hylonoma Hand-Mazz,which were hexadecyl-3-methoxy-4-hydroxybenzeneacrylate Ⅰ,ethyl brevifolincarboxylate Ⅱ and(+) 3′-angeloyl-4′-isovalerylcis-khellactone peuformosin Ⅲ. Conclusion The above compounds were isolated from Euphorbia hylonoma Hand-Mazz for the first time,and the compound Ⅲ was the first obtained from the Euphorbiaceae.

AIM: To investigate the influence of laminin (LN) on the expression of bcl-2 in rabbit cornea endothelial cells. METHODS: Cultured rabbit corneal endothelial cells were divided into three groups: normal control group, LN group and negative control group. Immunohistochemical technique and ELISA were used to measure the staining and A value to assess the levels of bcl-2 expression. RESULTS: Bcl-2 expression score showed that LN group had a strong positive expression. Control group only showed a weak expression. Negative control group showed a negative expression by immunohistochemical staining. The mean A value of each group were 1.21?0.18 (LN group), 1.05?0.14 (normal control group) and 0.04?0.01 (negative control group). CONCLUSION: LN promotes bcl-2 gene expression in rabbit cornea endothelial cells, and protects the cells from apoptosis.

Objective To investigate the effects of low dose radiation on dendritic growth of newborn neurons in the hippocampus of young rat.Methods One month-old male rats were randomized into radiation group aind sham control group.Radiation group received whole brain irradiation at a single dose of 2 Gy.Retrovirus expressing green fluorescent protein (GFP) was used to label newborn neurons in the hippocampus through stereotaxic intracranial infusion.Immunofluorescence assays were performed to detect dendritic architecture alterations induced by irradiation at different time points.Results Compared with control group,the lengths of total dendrite and the longest dendrite significantly decreased at 2 and 4 weeks after irradiation (t =3.10,2.07,2.94,4.02,P ＜ 0.05).The branching points of new born neurons were also decreased significantly at 2 weeks post irradiation (t =2.23,P ＜ 0.05).The number of new born neurons reduced at 4 weeks post irradiation (t =8.43,P ＜ 0.05).Conclusions Low dose radiation could inhibit newborn neuron growth in the hippocampus of young rat,which may be one of the most important mechanisms involved in radiation-induced cognitive impairment.

Objective To explore the diagnosis and treatment of clinical possible organizing pneumonia.Methods The medical records of 31 patients with clinical probable organizing pneumonia were retrospectively analyzed.The clinical presentation,radiographic results and treatment were collected and analyzed.Results Thirty-one patients with non-response to antibiotics were preliminary diagnosed as organizing pneumonia.By percutaneous lung biopsy or transbronchial lung biopsy the tuberculosis,fungi,suppurative inflammation,lung cancer and other diseases were ruled out in 24 patients,and 7 patients were diagnosed by perfect effect of corticosteroids treatment.Twenty-one patients had typical CT findings.All the patients responded rapidly and completely to the administration of corticosteroids.Six patients relapsed after the reduction or stop of corticosteroid in a follow-up time of 6-25 months.Conclusions Non-response to antibiotics,the typical imaging findings and lung biopsy ruling out other diseases are important for diagnosing organizing pneumonia.Rapid response to administration of corticosteroids may be helpful to the diagnosis.Decreasing the dose of corticosteroid too early may cause recurrence of organizing pneumonia.