Objective To investigate the blood lead level and its related factors in children in Chongqing and to provide evidence for the prevention and treatment of lead contamination. Methods In June 2001, the blood lead levels in 173 children(aged 2～7 2 years old) selected from 2 kindergartens representing the children in urban district and suburb were detected. At the same time, the related factors were investigated by using questionnaires. Results The highest, the lowest and the mean blood lead levels were 270 ?g/L, 18 ?g/L and 77 73 ?g/L, respectively. The blood lead level of 23 70% children investigated surpassed 100 ?g/L(the international diagnostic criterion of lead poisoning in children). Significant regional difference in blood lead level was found. The mean blood lead levels in children in Yuzhong District and Dadukou District were 111 23 ?g/L and 57 57 ?g/L, respectively. The percentages of children in the two districts with blood lead levels higher than 100 ?g/L were 52 31% and 6 48%, respectively. Blood lead level was closely correlated with the dwelling condition, the dwelling location where factories were nearby or the number of factories was large, health habits, the frequency of eating canned food and popcorn, and culture levels of the mothers. Conclusion Blood lead level of children in the urban distict is significantly higher than that in the suburb, to which more attention should be paid.

Objective To characterize the effects of Sanchi on the alcoholic hepatopathy rats.Methods 70 SD male rats were randomly divided into five groups:normal group(n=10),control group(n=15),high-dose Sanchi group(n=15),low-dose Sanchi group(n=15),and Tiopronin group(n=15).In order to induce alcoholic hepatopathy,animals in the control,high-dose and low dose Sanchi,and Tiopronin groups were orally dosed with HongXing ErGuoTou(56% ethanol;5g/kg),corn oil(2ml/kg),and pyrazole(27.2 mg/kg)every morning for 14 weeks.Rats in high-dose Sanchi,low dose Sanchi,and Tiopronin groups,were treated every afternoon during the 14-week induction of hepatopathy,with 1.2g/kg Sanchi,0.6g/kg,and 100mg/kg Tiopronin,respectively.All rats were sacrificed 12 hours following the last injection.Blood samples were obtained prior to euthanization.Blood-fat and serum lipoidase activity were measured using an automatic blood biochemical analyzer.Blood haluronic acid(HA),laminin(LN)were determined by ELISA.Histopathological changes of hepatic tissues(i.e.steatosiss,inflammation,and fibrosis)were assessed by microscopic examination of HE and Masson staining of the right lobe of livers.Results Compared with rats from the normal group(treated with ddH2O),rats in the control group have significantly higher steatosis,inflammation,liver fibrosis,and increased level of serum cholesterol,HDL-C,LDL-C,haluronic acid,laminin and AST/ALT activity,(P0.05).More importantly,treatment with high-dose,low-dose Sanchi,or Tiopronin resulted in less severe steatosis,inflammation,fibrosis in the liver,and reduced the levels of serum haluronic acid,laminin(P0.05).Conclusions(1)Daily dosing of alcohol,corn oil,and pyrozole for 14 weeks faithfully replicated the alcoholic hepatopathy symptoms observed in the ALD model,such as hepatic steatosis,immune cell infiltration,blood-fat disturbance,increase in serum AST/ALT activity,and liver fibrosis.(2)Sanchi can significantly alleviate the symptoms in the alcoholic hepatopathy rats including steatosis,improve hepatic function and fibrosis.

[Objective] To observe the effects of regulating Qi and strengthening spleen on the patients of chronic ulcerative colitis.[Methods] 38 patients of chronic ulcerative colitis(UC)were randomized and divided into experiment group(20 patients)and control group(18 patients)according to the selected standard.The patients of experiment group were treated with complex prescription of regulating Qi and strengthening spleen,the patients of control group were treated with granules of Mesalazine.To observe the symptom of accumulated points and enteroscope appearance on two groups.[Results] The difference on the symptom of accumulated points of experiment group exceeded the control group(P0.05).[Conclusion] There is effect of regulating Qi and strengthening spleen on the patients of chronic UC.

Our aim was to assess the curative effect of endoscopic stenting in patients with pancreatic fistula. One patient suffered from pancreatic fistula was treated by pancreatic stenting drainage. The patient′s condition was significantly improved after endoscopic treatment, and no complications such as abdominal pain, bleeding, perforation, and elevation of serum amylase level were found. The fistula was cured in one week. Followed up for 5 months, no relapse of pancreatic fistula was found, and body weight of the patient increased 4kg. The results of B ultrasonography and abdominal X ray examination were also normal. So pancreatic stenting drainage is an effective and safe therapy for pancreatic fistula and should be the first choice.

Objective To remind the attention to contraceptive in cesarean section group by analyzing the treatment of 104 cases cesarean scar pregnancy (CSP).Methods From April 2009 to November 2012,104 patients with CSP treated in Department of Obstetrics and Gynecology,Wuhan Union Hospital were studied retrospectively.The incidence,treatment,and average in hospital days and average cost of hospitalization were analyzed.Results From 2009 to 2012,the number of CSP was 10,19,29,and 46 cases annually which accounted for the proportion of the corresponding period of gynecological patients were 0.33％,0.63％,0.97％,and 1.53％.Bilateral uterine artery embolization plus curettage was the most effective treatment.The average transfusion rate was 25％.The average amount of in-hospital days were (14 ± 8) days,the average cost of hospitalization were (12954.97 ± 3888.89) yuan.Conclusions The CSP incidence was increasing sharply in China.Although the current treatment methods could greatly reduce complications of CSP,it did harm to the patients and their family.The key to reduce the occurrence of CSP included reducing the rate of cesarean section,and strengthening contraceptive education after cesarean section.

Catharanthus roseus (L.) G. Don is a plant of the Catharanthus genus of Apocynaceae which has been reported to have therapeutic effects of detoxication and anticancer. In order to further study the alkaloid constituents of C. roseus, the aerial parts of the plant were extracted with 95% EtOH, and then treated with 2% H2SO4 and NH3H2O to obtain total alkaloids. The total alkaloids were separated and purified by column chromatography over silica gel and prepared by high performance liquid chromatography (HPLC). Their structures were elucidated on the basis of physicochemical properties and spectral data. A new alkaloid together with five known compounds were isolated and identified as vindolinine B (1), lochnericine (2), horhammericine (3), vindorosine (4), vindoline (5), and coronaridine (6). Compound 1 is a new compound and named as vindolinine B.

Objective: To explore the necessity for application of bone wax stanching bleeding at sternal edge in cardiac surgery by median sternotomy. Methods: A total of 445 patients who receive cardiac surgery by the same surgeon performing median sternotomy in our hospital from 2011-01 to 2014-12 were studied. According to application of bone wax stanching bleeding, the patients were divided into 2 groups: Bone wax group,n=210 and Non-bone wax group,n=235. Re-thoracotomy for hemostasis, 12 h, 24 h and total draining volume after the operation and time of closing incision were compared between 2 groups. Results: There were 2 patients and 1 patient received re-thoracotomy for hemostasis in Bone wax group and Non-bone wax group respectively, which was not related to bone marrow cavity bleeding. In Bone wax group and Non-bone wax group, the draining volume at 12 h post-operation were (451 ± 240) ml vs (483 ± 238) ml, at 24 h post-operation were (615 ± 304) ml vs (639 ± 285) ml and the total volume were (842 ± 467) ml vs (842 ± 364) ml, allP>0.05; the time of closing incision were (68.0 ± 23.0 ) min vs (66.0 ± 19.0 ) min,P>0.05; the total transfusion rates were 21% vs 19%,P>0.05. Conclusion: Application of bone wax at sternum edge could neither affecting the drainage nor the speed of closing incision in cardiac surgery and therefore, it might be given up.

BACKGROUND: Heal-all oral biofilm is a material utilized in repairing oral mucosa and soft tissues defects and characterized by degradation, easily preparation, long preserved duration, convenient transportation and good ossification, which has been widely used in dental implant as guided bone regeneration materials.OBJECTIVE: To check the clinical effective of Heal-all oral biofllm on guided bone regeneration in dental implant.METHODS: A total of 72 patients with bone defects in the implantation area were selected as subjects, who were divided into control group and experimental group at random. Bone defects around implants were repaired by guided bone regeneration technique with BME-10X medical collagen membrane and Heal-all oral biofilm respectively. X-ray and clinical examination were taken at 1 and 3 months after implantation. The amount of new.formed bone tissue was evaluated when stage Ⅱ operation was performed.RESULTS AND CONCLUSION: In stage Ⅱ operation, osseointegration was formed between implants and bone tissue in all 72 patients. The average rate of bone formation was 92% in the experimental group while 91% in the control group. All implants were successfully repaired with implant denture. Occlusal function was restored successfully with all 72 implants during the follow-up period of 3-24 months after restoration. As an alternative option of BME-10X medical collagen membrane, Heal-all oral biofilm can be used in guided bone formation clinically.

0.05) The above amount was enough to maintain the subjects two weeks in good vitamin C nutrition as judged by steady plasma ascorbic acid content of about 1 mg%. At the end of maintenance period, 400mg of ascorbic acid was administered. The urinary excretion of ascorbic acid in 24 hours was about 30% of the test dose.This method is reliable but not suitable for large scale analysis as it involves daily urine collection and analysis.The urinary excretion of ascorbic acid decreased during menstrual period in women subjects.

Objective: To observe the effect of representative formula of nourishing yin and invigorating the kidney(Liuweidihuang Granula) cooperating with Flixotide aerosol inhalation on the allergic airway inflammation of asthma rat models.Methods: Wistar rats were randomly divided into six groups.Except the norma1 control group,all the other 5 groups were sensitized by injecting into the abdominal cavity of the ovalbumin(OVA) together with aluminum hydroxide as adjuvants,2 weeks later followed by aerosolized OVA challenge to prepare the asthma models.From the eighth day of the experiment,in medication administration teams, rats were sprayed with Flixotide aerosol and given intragastric administration with Liuweidihuang Granula.The normal control group and the asthma model group were drenched with normal saline.Results: There was no difference between the groups given Liuweidihuang Granula and different dosages of Flixotide aerosol but the amount of IFN-? and IFN-?/IL-4 in BALF in the Liuweidihuang Granula cooperating with Flixotide aerosol group were higher than that in the model group(P