The effect of molecular cytogenetics in pathogenesis of multiple myeloma (MM) is fundamental.The risk stratification system that based on molecular cytogenetics of MM is widely applicable in the prognosis of MM.The aberration of molecular cytogenetics is the most important marker of MM.At the same time,high-risk MM is associated with intra-clonal tumor heterogeneity and clonal evolution.The research of intra-clonal tumor heterogeneity and clonal evolution which based on the aberration of molecular cytogenetics will provide some additional information of the biology of MM and contribute to the optimum treatment for MM patients.

Objective To investigate the relation between body mass index(BMI),skinfolds(SF)and relative weight(RW),arm girth(AG) and ag e of the children in order to look for the most suitable index used to diagnose children obesity in clinical work.Methods In 1496 healthy children (ranging from 1 to 13 years),their weight, height,AG, triceps skinfold(TSF), sub scapular skinfold(SSF) and abdominal skinfold(ASF) were measured and the relat ion of BMI,SF,RW,AG and age was analyzed.Results 1.Between BMI and the sum of three skinfolds(SF3) ,RW,AG,correlation coefficient was more t han 0.8(P＜0.001).2. With the increase of age, AG and ASF increase wh ile TSF and BMI decrease. 3.Skinfolds in girls of 3～6,12 years of age are thicker t han those in boys;BMI in boys of 8,9 years of age are higher than those in girls .Conc lusions 1.95 percentile of BMI and SF3 as index to diagnose children obes ity is better than single application of RW or one-site skinfold. 2. With the i ncrease of age, AG and abdominal fat increase, but triceps and total fat of body relatively decrease. 3. Fats in girls develop faster than in boys.

[Objective] To compare and analyse the clinical effect of tiny incision blind-cut suck-erasion on unhairing and preserving armpit hair in bromhidrosis.[Method] Select 429 cases of 851 sides in our hospital, divide them into treatment group(blind-cut suck-erasion+unhairing) 308 cases with 609 sides, and control group(blind-cut suck-erasion+preserving armpit hair) 121 cases with 242 sides. Make statistics and compare their complications in recent and forward stages, also the recurrence and residual of peculiar smel after 1 year. [Result] For the treatment group, 38 sides had the recent compli-cation of hematoncus, 30 sides had partly skin necrosis, 12 had bad cut cure, 70 had early slight hardening and contracture skin; for the forward complica-tions: 27 sides had focal slight scar, 3 severe scar, 23 focal subcutaneous sac lump with infection, 3 peculiar smel residual and recurrence. To the control one, for the recent complications, 11 sides had hematoncus, 9 partly skin necrosis, 5 bad cut cure, 13 early slight hardening and contracture skin; for for-ward complications, 8 sides had focal slight scar, 1 severe scar, 3 focal subcutaneous sac lump with infection, 38 peculiar smel residual and recurrence. By comparison, on early slight hardening and contracture skin and focal subcutaneous sac lump with infection, the treatment group was higher than control one;other complications had no difference of statistical meaning;on radical cure rate, the treatment group was obviously more than control one, with dif-ference of statistical meaning. [Conclusion] The tiny cut blind-cut suck-erasion is definite in treating bromhidrosis, the operation removes subcutaneous apocrine sweat gland, meanwhile removes the hair fol icle and sebaceous gland complexus, the therapy is very thorough, with better cure effect, less recur-rence, without increasing complications.

Objective To study the expression of PI3K and Akt mRNA in acute leukemia(AL) and chronic myelocytic leukemia (CML) and try to find out a relationship between the prognostic significance of acute leukemia and the expression level of PI3K and Akt. Methods The samples were collected from 63 ALpatients, 7 relapsed AL patients, 14 CML patients and 10 AL patients in complete remission(CR) patients, 11samples of normal controls(NC). The expression of PI3K, Akt mRNA were measured by semi-quantity reverse transcription polymerase chain reaction(RT-PCR). Results The expression of PI3K mRNA in AL and relapse group were significantly higher than that in normal control. In CML, the P13K mRNA expression level was higher than that in NC and CR. The similar results of the expression of Akt mRNA were observed in CML, NC and CR. At the same time, the expression level of Akt mRNA in relapse group was significantly higher than that in NC. The complete remission rate in PI3K (+) was lower than that in PI3K (-). Similar result was obtained in Akt (+) and Akt (-). Conclusion The PI3K/Akt pathway may contribute to the occurrence of leukemia. The patients with positive expression of the members of this pathway had a lower remission rate.

Objective To establish a neonatal transport network in order to improve the diagnosis and treatment levels of the basic medical unit and to decrease medical disputes and fatality rate of the new born Methods The first neonatal transport network in the Southwest of China, covering Chongqing and the adjacent areas, was established During the process of transport, warm keeping, unobstructed respiratory tracts, oxygen treatment, establishment of venous passage and placement of gastric canal were included Meanwhile, the vital signs, colors of the skin and mucosa and consciousness should be closely observed Corresponding measures could be taken at any time when needed Results Satisfactory results were achieved by means of the neonatal transport network Conclusion The neonatal transport network can play a very important role in strengthening the treatment of the newborn, decreasing the fatality rate and sequela and improving the diagnosis and treatment levels of the basic medical unit

Objective To analyze labor duration in nulliparous women and discuss the change about curve of labor duration. Methods Two thousand, one hundred and forty nulliparous, full-term pregnant, singleton, cephalic presentation and vaginal delivery women who delivered at Peking University First Hospital were included. There were 1 300 cases between January 1, and December 31, 2009, while 840 cases between January 1, and May 31, 2013. A retrospective study was conducted. Data on maternal age, gestational age at delivery, body mass index at delivery, newborn weight, time of labor initiation, cervical dilatation and the amount of bleeding within the first 24 h after delivery were recorded. Data were compared by t test and χ2 test. The median time span corresponding to one-centimeter-increase in cervical dilatation was calculated. Results (1)Compared with data from 2009, the maternal age [(29.0±3.0) vs (29.6±2.8) years, t=4.77], incidence of postpartum hemorrhage [1.8%(24/1 300) vs 4.3%(36/840),χ2=11.17], proportion of induced labor [37.3%(485/1 300) vs 64.0%(538/840),χ2=146.23] and proportion of analgesia during labor [54.2%(705/1 300) vs 61.5% (517/840), χ2=11.15] were all higher in 2013 (all P<0.01). (2)The median (minimum–maximum) time span corresponding to a one-centimeter-increase in cervical dilatation was 3-4 cm which corresponded to 2.2 h(0.8-4.3 h), 4-5 cm which corresponded to 1.9 h(0.6-4.0 h), 5-6 cm which corresponded to 1.8 h(0.5-4.0 h), 6-7 cm which corresponded to 1.6 h(0.5-2.0 h), 7-8 cm which corresponded to 1.8 h(0.8-2.0 h), 8-9 cm which corresponded to 1.3 h(0.2-2.5 h), and 9-10 cm which corresponded to 0.6 h(0.1-2.5 h). The curve of labor duration showed a slow uptrend with time on the horizontal axis and cervical dilatation on the vertical axis. Conclusions The curve of labor duration exhibits a slow uptrend with neither an acceleration phase nor a deceleration phase. It is important to redefine the time span of labor duration in China for appropriate clinical treatment.

Objective To study the Janus Kinase 2 V617F (JAK2V617F) point mutation in bcr-abl-negative myeloproliferative disorders (MPD) and explore its clinical significances. Methods Genomic DNA was isolated from bone marrow or peripheral-blood granulocytes. Allelespecific-polymerase chain reactions (AS-PCR), restriction enzyme digestion in combination with PCR product sequencing were performed to detect the mutation in genomic DNA. 110 patients were detected, including 41 with bcr-ablnegative MPD, 25 with bcr-abl-positive chronic myelogenous leukemia (CML), and 44 with acute leukemia.Results JAK2V617F was presented in 11 cases(91.7 %) of 12 polycythemia vera (PV), 8 cases(53.3 %) of 15 essential thrombocythemia(ET), 4 cases (57.1%) of 7 idiopathic myelofibrosis (IMF), while in other patients including 7 hypereosinophilic syndrome (HES), 25 bcr-abl-positive CML, 24 acute myelocytic leukemia (AML), 18 acute lymphoblastic leukemia(ALL), and 2 acute mixed lineage leukemia, JAK2V617F can not be detected. All positive samples and 10 negative samples identified by AS-PCR and restriction enzyme digestion were confirmed further by DNA sequencing. Conclusion The frequency of JAK2V617F mutation was more than 90 % among patients with PV, more than 50 % among patients with ET and IMF. The detection of JAK2V617F mutation will be of great significanees in the diagnosis and differential diagnosis of MPD. This mutation can be a molecular marker of MPD and might be a treatment target in the future.

OBJECTIVE To explore an effective cleaning method to improve the quality of sterilization,in order to control infections in the hospitals. METHODS To adopt orthogonal experimental forms of L_9(3~4) and take water temperature,cleaning agent type and cleaning method as experimental factors,in this way to investigate decontamination method for surgical instruments,using TOSI indicator cards and Browne STF load device to test the effectiveness of cleaning results. RESULTS It showed that by KQ-4200SY medical digital full-automatic ultrasonic cleaner with washing water at 33-35℃ an optimal cleaning result would be obtained. CONCLUSIONS It is practical to clean surgical instruments by KQ-4200SY medical digital full-automatic ultrasonic cleaner,and by TOSI indicator cards and Browne STF used as test method.

Cell adhesion is a basic and very important tissue in the field of tissue engineering. Fibronectin and integrins are the most important elements to cell adhesion. Some surface receptors of fibroblast can also conjugate with type I collagen in extracellular matrix (ECM) directly. Laminin receptors on the surface of fibroblast bound to laminin also play a role in cell adhesion. In this paper are reviewed a number of related articles. The structures and function of fibronectin and integrins are discussed in detail; the tendon cell's adhesion structures are also discussed. Yet, there was scarcely any paper on the effects which the preservation of tissue engineered products may have on cells' adhesion fo ECM. Therefore, researching on cell adhesion and finding a way of preservation that has no or very little adverse effect on cell adhesion is an important topic. Results from expected advanced researches on cell adhesion may probably find promising applications in the field of tissue engineering.
Cell Adhesion ; Extracellular Matrix ; metabolism ; Fibroblasts ; cytology ; Fibronectins ; metabolism ; Humans ; Integrins ; metabolism ; Laminin ; metabolism ; Tendons ; metabolism ; Tissue Engineering

This study sought to find out a good way for the cryopreservation of tendon seeding cells so as to facilitate the preparation of tissue engineering tendons as products. The related questions are how different factors affect cell survival rate at the procedure of preservation and whether cryopreservation affects seeding cells' biological characters as well as collagen secretive function. The results of experiment indicate that DMSO is a more effective cryoprotectant in cryopreservation of tissue engineered tendon seeding cells. Blood serum nourishment is very important in cell culture, preservation and treatment. The same sustenance after cryopreservation increases cell survival rate. In the process of cryopreservation, the concentration of cells is important to cell survival rate; cell survival rate will decrease when it is less than 1.0 x 10(6)/ml. In the process of cryopreservation, the cooling speed is also important to cell survival rate, slow cooling method achieves higher cell survival rate than does the rapid cooling method. Cryopreservation by use of 10%DMSO+15%FCS+75%DMEM does not affect seeding cells' collagen secretive function greatly and does not affect seeding cells' growth curve, cell cycle and chromosome mode obviously. The prescription of 10%DMSO +15%FCS+75%DMEM is suited for the cryopreservation of tendon seeding cells.
Cell Count ; Cell Survival ; Cryopreservation ; methods ; Dimethyl Sulfoxide ; Humans ; Muscle, Skeletal ; cytology ; Tendons ; cytology ; Tissue Engineering ; Tissue Preservation ; methods