OBJECTIVETo develop a nebulizer controller which can be used to control the ultrasonic nebulizer and spray atomizer which can optimize the working of the nebulizer in pulse way, in a purpose to save the medication and minimize the impact on the environment.

METHODSDesign the controller by using SCM to adjust the frequency and duty factor of the nebulizer pulse. The output pulse of the controller can drive a relay or a electromagnetic valve to control the pulse work of the two kind of nebulizer.

RESULTSThe controller can work in a pulse way, and patients can inhale in pace with the nebulizer.

CONCLUSIONThe controller can save the medication by 50%, and reduce the impact on the environment by 50%.

Equipment Design ; Metered Dose Inhalers ; Ultrasonics

In the teaching reform of anatomy,department of human anatomy in Heze medical college put forward a new teaching means-Non-verbal communication teaching.The reasonable use of the paralanguage,gesture,posture,eye contact,facial expressions can optimize the teaching of anatomy and achieve good teaching effect.

Objective To isolate and purify human coagulation factor Ⅶ from Cohn fraction Ⅲ precipitate.Methods The purification procedure of human factor Ⅶ from Cohn fraction Ⅲ precipitate involves dissolving fraction Ⅲ,absorbing factor Ⅶ onto barium citrate and eluting,ammonium sulfate fractionation and DEAE-Sepharose Fast Flow ion exchange chromatography,and Sephadex G-100 gel filtration chromatography.Results 10.1mg purified FⅦ was obtained from 400g Cohn fraction Ⅲ precipitate.The purified FⅦ has a specific clotting activity of 1775.8U/mg and the overall yield of FⅦ specific clotting activity is 17.6% of the starting material.The purity of FⅦ was judged by SDS-PAGE and there was only one protein band on the gel.Conclusion The procedure of purifying Ⅶ from Cohn fraction Ⅲprecipitate is established with satisfactory purity.

OBJECTIVETo develop a spectral-domain optical coherence tomography (SD-OCT) system, for precisely imaging the accommodation of the anterior segment of the human eye.

METHODSDesign the SD-OCT system based on two Michelson interferometer by using the basic principal of weak coherent light interference and modular design of dual channel dual focus.

RESULTSThe system can provide simultaneous cross-sectional imaging of all the surfaces of the anterior segment of the eye including the cornea, anterior chamber, anterior and posterior surfaces of the crystalline lens. Thus, the dynamic change of modification of the curvatures can be calculated in real time.

CONCLUSIONSThe system was successfully tested in imaging accommodation, with high resolution and non contact. The preliminary results demonstrated the feasibility of this novel approach to measure the eye parameters.

Accommodation, Ocular ; Anterior Eye Segment ; physiology ; Humans ; Tomography, Optical Coherence ; methods

Background: Previous study has found that ursolic acid (UA) inhibited the proliferation of gastric cancer cells by the down-regulation of cyclooxygenase-2 (COX-2) expression.However,its molecular mechanism is not fully clear.Aims: To investigate the role of adenosine monophosphate-activated protein kinase (AMPK)/signal transducer and activator of transcription 3 (STAT3)/COX-2 signaling pathway in UA-mediated inhibition of gastric cancer cells proliferation.Methods: AMPK-pLVX,AMPK-shRNA,STAT3-pLVX,STAT3-shRNA plasmids were constructed,and then were transfected into human gastric cancer cell lines SGC-7901 and MKN-45,respectively.Gastric cancer cells were cultured with different concentrations of UA for different times.The expressions of phosphorylated AMPK (p-AMPK),phosphorylated STAT3 (p-STAT3) and COX-2 were measured by Western blotting,and cell proliferation was detected by CCK-8 assay.Results: UA dose-and time-dependently increased p-AMPK expression,inhibited p-STAT3 and COX-2 expressions in SGC-7901 and MKN-45 cells.Knockdown of AMPK blocked UA-induced inhibition of STAT3 phosphorylation and COX-2 expression.Overexpression of STAT3 blocked UA-induced down-regulation of COX-2 expression.Knockdown of AMPK and overexpression of STAT3 blocked UA-induced inhibition of proliferation of gastric cancer cells.Conclusions: UA may inhibit the proliferation of gastric cancer cells via down-regulation of COX-2 expression through AMPK/STAT3 pathway.