Tumor angiogenesis, resulting from the imbalance of angiogenic cytokines and angiogenesis inhibitors, is crucial for tumor growth and metastasis. As a new field of cancer therapy, tumor anti-angiogenesis therapy with regard to abrogation of tumor vasculature has become an important anti-tumor strategy. Gene therapies have the potential to provide antiangiogenic mediators specifically targeted to organs containing tumors and make a great improvement in this therapy. This article reviews the current understanding of tumor angiogenesis, with a focus on gene therapy in this field.

Objective To investigate the effects of Atrovent aerosol inhalation in the treatment of respiratory failure and nursing intervention of chronic obstructive pulmonary disease effect. Methods January 2016 to December 2016 in Huzhou Central Hospital for diagnosis and treatment of chronic obstructive pulmonary disease patients with respiratory failure in 50 cases dewided into control group and observationgroup: the control group treated with conventional methods, the observation group treated with Atrovent aerosol inhalation therapy and conventional methods; the patients in the two groups of experimental data were recorded in detail, and the obtained data were analyzed. Results The observation group was better than that of treatment and intervention control group, clinical symptoms improved better than the control group, the adverse reaction rate were lower than the control group, there is significant statistical difference (P<0.05). Conclusion The patients with chronic obstructive pulmonary disease with respiratory failure choose conventional and Atrovent aerosol inhalation treatment effect significantly, can effectively improve the patient clinical symptoms and adverse reactions are few and deserve to be widely used in the treatment of chronic obstructive pulmonary diseaseand respiratory failure patients.

Alteration in gastric emptying may play a role in the pathogenesis of many diseases.And various methods to measure gastric emptying have been developed.Nine procedures for the assessment of gastric emptying were reviewed in this paper including intubation test,radiology method,absorption test,real time ultrasound,breath test,radionuclide technique et al .Among all of these methods,radionuclide technique is considered as the good standard method.

Observations are made on 1208 postoperative cases and logistic analyses are centered on the risks related to postoperative hospital infections.It is discovered through observations that the rate of hospital infections is 7.5%.The infected parts are mainly the lower respiratory tract and the inci- sion.Logistic analyses of the risks indicate that postoperative hospital infections are related to preopera- tive hospitalization duration,the time span of endotracheal intubation,trachealaryngotomy and the re- tention of pleural drainage,type of operation,operational duration,and postoperative complication. Thesefore,hospitals should look seriously at the postoperative infection risks and take important steps to control them.

Physical constraints is a commonly protective medical measure in clinic. if lack of humanistic nurs-ing in the use of the process,then it will give the patient a physical,psychological and ethic issues. This paper sum-marized the research status of humanistic nursing and physical constraints and concluded the humanistic nursing measures before, during, and after physical constraints, and thus to provide a reference for the further implementa-tion of humanistic nursing.

Objective: To investigate the awareness and training needs of incontinence-associated dermatitis (IAD) among clinical nurses, so as to provide insights into the standardization of clinical nursing of IAD. Methods: Clinical nurses with more than one year of service in Lanxi People's Hospital were selected as study subjects in October 2021. The demographics, awareness of IAD knowledge and training needs were collected using a self-designed questionnaire. The age-, duration of service-, academic degree-, professional title- and department-specific awareness of IAD knowledge was analyzed. Results: A total of 420 questionnaires were allocated, and 409 valid questionnaires were recovered, with an effective recovery rate of 97.38%. All respondents were females, with a mean age of (37.68±4.82) years, and a mean service duration of (18.72±3.46) years. There were 199 nurses with a primary title (48.66%), 169 nurses with a intermediate title (41.32%), and 41 nurses with a senior title (10.02%). There were 217 nurses with a bachelor degree or above (53.06%), and 208 nurses from internal medicine and surgery departments (50.86%). The overall awareness of IAD knowledge was 74.57% among the respondents, and the awareness rates of IAD definition, the difference between IAD and pressure ulcers and influencing factors of IAD were 86.06%, 83.37% and 82.15%, respectively. The nurses at ages of 41 to 53 years (89.01%), having a senior title (94.24%), with a nursing duration of 21 years and longer (92.97%) and participating in IAD training during the recent one year (77.41%) had a high awareness rate of IAD-related knowledge. There was a high proportion of needs for training on basic IAD-related knowledge (396 nurses, 96.82%). Conclusions The awareness of IAD knowledge requires to be improved among clinical nurses. The training on IAD knowledge is recommended to be improved.

According to the large stray capacitance of the gate terminal of power MOSFET,this paper introduces the main characteristics and application of IC driver MAX626/7/8,especially designed for the high speed power MOSFET.The ultrasound generator made from that chip is simple in circuit,stable when working as well as small and light for carrying.

Malignant transformation is closely related to gene mutations.The tumor suppressor gene p53 codes for a transcription factor,P53 protein,plays a critical regulation role in oncogene expression,DNA synthesis and repair systems,and cell apoptosis.It has been demonstrated that p53 mutation occurs in more than 50% of cancer cells,which is thought to play a crucial role in malignant transformation.p53-based gene therapeutical approaches in vitro and in vivo aiming at a direct correction of the specific molecular defect in p53-mutated malignant cells,showed notable efficacy in numerous cancer types.In this review,we discussed the current advances in this field,focusing on adenovirus-mediated wild-type p53 gene therapy.

Objective To explore the inhibitory effect of genistein on oncogene HER-2/neu-overexpressing breast cancer xenograft tumors and its relationship with urokinase type plasminogen activator (uPA) expression. Methods HER-2/neu-overexpressing MCF-7 human breast cancer cells (MCF-7/HER-2) were generated by transfecting the HER-2/neu negative MCF-7 cells with HER-2/neu cDNA. MCF-7/HER-2 and MCF-7 xenograft models were established in BALB/c nude mice, and the nude mice with MCF-7/HER-2 xenograft were randomly divided into three groups: control group, genistein-treated group, and HER-2/neu antibody-treated group. The growth ability of xenograft tumors was analyzed by immunohistochemical staining with Ki-67 antibody, and uPA expression of xenograft tumors was analyzed by Western blotting. Results The growth ability and uPA expression were significantly higher in MCF-7/HER-2 xenograft tumors than those in MCF-7 xenograft tumors. MCF-7/HER-2 xenograft tumors treated by genistein or HER-2/neu antibody showed lower growth ability and uPA expression as compared with MCF-7/HER-2 xenograft tumors. Conclusion Genistein can inhibit the growth ability of HER-2/neu-overexpressing breast cancer xenograft tumors, which is associated with the uPA expression.

Objective: To study the effects of genistein on the expression of urokinase-type plasminogen activator (uPA) and the activity of protein tyrosine kinase (PTK) in MDA-MB-453 cells, and explore the molecular mechanism of anti-angiogenesis in HER-2/neu-overexpressing breast cancer by genistein. Methods:Western blot, immunoprecipitate, reverse transcription-polymerase chain reaction (RT-PCR) and kinase activity analysis technics were used to observe the expression of uPA and the protein phosphorylation of HER-2/neu receptor and the activity of protein tyrosine kinase (PTK) in MDA-MB-453 cells treated by genistein for 24, 48, 72 h. Results: The expression of uPA and the protein phosphorylation of HER-2/neu receptor and the activity of PTK were significantly decreased after treated with 5?10-5mol/L genistein, which had a time-dependence. Conclusion: Genistein could inhibit the activity of PTK and the protein phosphorylation of HER-2/neu receptor, and down-regulate the exprssion of uPA at transcription and translation levels in breast cancer cells. This might be a part of molecular mechanism of genistein anti-angiogenesis in HER-2/neu-overexpressing breast cancer.