Objective: To observe the position change of the upper mol ar s and incisors in cases with Angle class I malocclusion and crowding treated by extraction of four first premolars. Methods: 20 patients with An gle class I malocclusion and crowding aged 14-16 years old, 8 male and 12 femal e, were treated by extraction of the four first premolars, and Edgewise applianc e. Two X-ray cephalometry films were taken for every patient before and after t reatment, cephalometry measurements were obtained and the position of the upper molars and incisors were analyzed. Results:The treatment was co mpleted in 18 month on average in all patients. After treatment, the crown of th e upper first molars showed mesial displacement of 3.15 mm on average, the crown of the upper incisors distal displacement of 2.55 mm, the crown of the lowe r first molars mesial displacement of 4.3 mm, the crown of the lower incisors d istal displacement of 1.77 mm. Conclusion: With of dental anchor age, the position changes of the upper molars and incisors in the cases with Ang le class I malocclusion and crowding after extraction treatment may be achived.I n relation to RL line,the mesial displacement of molars are larger than the dist ally displacement of incisors.

Objective To study recent clinical efifcacy and safety analysis of tinidazole and metronidazole in treatment of severe oral infection. Methods, 88 cases with severe oral infection(SOI) collected in the third people's hospital of Wenling from April 2012 to April 2013 were divided into observation group (n=45) and control group (n=43) by random number table. Control group were received metronidazole drug treatment, while observation group were treated with tinidazole medication, The efifcacy, recovery duration and adverse reactions were compared between two groups. Results The efficacy of observation group was significantly better than control group, the difference was statistically significant (P<0.05). The average duration of symptoms and wound healing in observation group were (4.8±0.4)hs and (4.3 ± 0.2)hs, which were signiifcantly less than (5.5 ± 0.2)sh and (5.6 ± 0.1)sh in control group(P<0.05).Adverse reactions in observation group were signiifcantly lower than that in control group (P<0.05). Conclusion Tinidazole has a signiifcant effect and less adverse reactions in treatment of SOI.

Objective To investigate the correlation between adiponectin, CRP and homocysteine (Hcy) and evaluate its mechanism in acute cerebral infarction. Methods Serum adiponectin, hs-CRP,Hcy, blood lipid, FPG and blood pressure were measured in eighty patients with acute cerebral infarction ( cerebral infarction group) and eighty healthy persons ( control group). Results In cerebral infarction group, serum hs-CRP, Hcy, TG , and systolic pressure were significantly higher than in control group ( P ＜0. 05) , while serum adiponectin were significantly lower than in control group ( P ＜0. 05). Serum adiponectin levels were negatively correlated with Hcy( r = -0. 453) , hs-CRP ( r = -0. 397) ,TG( r = -0. 321) , FPG( r = -0. 318) and systolic pressure ( r = -0. 288). Conclusion There is correlation between adiponectin, Hcy, hs-CRP, glucose and lipid metabolism and blood pressure, which plays a role in occurrenc and development of acute cerebral infarction.

Objective To investigate the role of PT H (1-34 ) on the expression of receptor activator factor of NF-κB ligand (RANKL) in the induction of osteoclasts and its effect to osteoclasts on compression side during the repairs of tooth root in rats model of tooth resorption by intermittent injection of small dose of PTH (1-34) .Methods After the model of tooth resorption was established in rats 6-8 weeks in age ,63 male SD rats were divided in three groups .Rats in the control group were not given injec-tions for any drugs ;The negative control group were given injections for normal saline 6μg/kg subcutaneously every other day ;The experimental group were given injections for PTH (1-34) 6 μg/kg(PTH :1 μg/mL) subcutaneously every other day ;then rats in every group were killed on the day 0 ,7 ,10 ,14 ,17 ,21 ,25 .TRAP staining for counting TRAP-positive stained osteoclasts ,calculat-ing the mean ;Ligand RANKL immunohistochemistry and using image-pro-plus image analysis system to measure the average opti-cal density value of compression side .Results On the day of discontinuation ,the tooth resorption continued in each group ;the num-ber of osteoclasts between every two arrays there were no significant statistic differences (P>0 .05);RANKL immunohistochemis-try :Compared with control group and the negative control group ,the experimental group significantly increased in early stage ,and reduced in latest stage(P<0 .05) .Conclusion It indicated that intermittent injection of small dose PTH (1-34) did not cease the rats tooth resorption which occured during the period of tooth repair .

Objective:To analyze the long term stability of the hard and soft tissues of the skeletal class III malocclusion after ortho-dontic-surgery by Meta-analysis.Methods:The randomized controlled trials(RCT),quasi-randomized controlled trials and clinical case-control trials(CCT)about the long-term stability of skeletal class III malocclusion after orthodontic-surgery was collected from Co-chrane Library,PubMed,Medline,CNKI,VIP and Wanfang databases from inception to December 31,2013 by literature search.Two professionally trained reviewers evaluated the quality of the screened literatures,scored literatures with the NOS evaluation tool,and collectd data and information of the literatures with medium and higher quality.Revman5.2 was used for the Meta-analysis.Results:4 CCTs articles and 178 cases were included.The results of Meta-analysis showed that:1.The stability of the hard tissue:the differ-ence of SNA(WMD =-0.58,95%CI[-1.18,0.02]),SNB (WMD =0.25,95%CI[-0.42,0.91]),ANB(WMD =-0.70, 95%CI[-1.44,0.04])and MP-SN(WMD =0.39,95%CI[-0.25,1.04])at post-operation and 3 years after operation had no statistical significance.Point A remained relatively stable position and point B had some replace 3 years after surgery compared with that at post-operative.2.The stability analysis of soft tissue:the healing effect of the soft tissue was stable,except the LowerlipeNperp had some recurrence 3 years after surgery.Conclusion:In the long term,after orthodontic and surgical treatment for skeletal class III malocclusion the hard and soft tissues kept stabile except a little replapse of the mandibular and the LowerlipeNperp.

[Abstract ] Objective The orthodontic-surgery is the most effective way to treat the severe skeletal class Ⅲmalocclusion,but the long-term stability is still disputed .The aim of this systematic review is to analyze the long-term stability of hard tissue of seriously skeletal Class Ⅲ malocclusion patients treated with orthodontic combined surgical treatment . Methods Literature were searched through the Cochrane Central Register of Controlled Trials , Cochrane Library, Medline via pubmed (1950-2014), EMBASE (1980-2014) and other foreign databases , and Chinese Biomedical Literature Database , China National Knowledge Infrastructure Database , VIP Database for Chinese Technical Periodicals , digital journal of Wan fang Data and so on .Unpublished conference papers and gray litera-tures were collected manually .The literatures consist of randomized controlled trials ( RCT) , quasi-randomized controlled trials and clini-cal case-control trial (CCT) were selected.Then meta analysis was performed for annexable literatures and qualitative description was performed for diverged documents . Results Five foreign articles and 1 Chinese article suitable for analysis were ultimately studied . All the studies were CCT and a total of 260 patients were involved in the present systematic review .Meta analysis showed that the SNA , SNB, ANB, MP SN and Y-axis did not change significantly during 3 years after operation (P>0.05) and a good skeletal class I facial types were maintained .The comparison results of MP-SN and Y-axis showed that there was no significant statistical difference and the mandibular plane angle maintained the relative stability .Meta analysis was not performed because of the different measurement methods of A, B, Pg and Ramus inclination , so the qualitative description was used .Point A and Ramus inclination remained relatively stable posi-tion, but point B and Pg had some replace compared with post-operative. Conclusion The orthodontic and surgical treatment for skeletal classⅢmalocclusion could keep the hard tissue relative stabil-ity except a little replace of the mandibular .

Condyloma acuminatum is closely associated with human papillomavirus (HPV). In this study the method of slotting hybridization was used to detect the Specimens of 28 cases of condyloma acuminatum. Fourteen out of the 28 samples were positive for HPV 11 probe, the positive rate was 50% (14/28). Nine out of the 14 positive samples were digested with Bamll Ⅰ and Pst Ⅰ respectively to study the present state of DNA after using Southern blotting hybridization. It was shown that HPV 11 DNA were mainly present in a simple monometric episomal state in the condyloma acuminatum cells.

The transformation of human papillomavirus type 16 (HPV16) may play somc important roles in the oncogenesis of cervical carcinoma Studies showed that the transforming gence mainly located in the E6. E7 open reading frames (ORFs). We digested HPV16 DNA with PstI+EcoRI. Using pUC-19 as the vector, E. coli JM103 strain as the host cell, after two times of directive subelone, plasmid pEP-8 was construeted, confirmed by RE analysis and Southern blot hybridization, the inserted band of pEP-8 was 1.43Kb long contained the intact E6 E7 ORFs, and also a cat-box region, two TATA-boxics and a cell-type specific cnhancer in the upstream swquence. The pEP-8 could be used as the specific probe of HPV-16 E region for identifing virol mRNAs in the HPV infected cells. Meanwhile it was a basework for studying the transformation and transforming proteins of HPV-16.

OBJECTIVEThis study aims to analyze the long-term stability of the anterior teeth and hard tissue of skeletal class III malocclusion after a three-year orthodontic surgery by systematic review.

METHODSAll studies about skeletal class III malocclusion with orthodontic-surgery were searched by computer-based retrieval and manual retrieval; the deadline is December 2013. The literature, filtered according to the inclusion criteria and exclusion criteria, was performed with quality. assessment. The same indicators of the anterior location and hard tissue stability were combined and evaluated with metaanalysis and descriptive analysis by Rev Man5.2.

RESULTSFour before-and-after comparison study articles with 180 cases were included. The grades of the four literature evaluation were A. The meta-analysis results showed that comparing the three-year post-orthodontic-surgery and post-orthodontic-surgery, the total weighted mean difference (WMD) of Ul-SN was 4.29 (P<0.05); the WMD of Ll-MP, OB, OJ, SNA, SNB, ANB, and MP-SN were -1.58, 0, -0.41, -0.58, 0.25, -0.70, and 0.39, respectively (P>0.05). The measurement methods of A and B point position were different, hence the qualitative description were as follows: point A remained at a relatively stable position, and point B had some replacement compared with post-operative (P<0.05).

CONCLUSIONTo the skeletal class III malocclusion after three-year orthodontic-surgery, the position of the lower anterior teeth could be kept stable, as well as the overbite and the overjet of the anterior teeth; only the upper inci- sor has a lip-inclined relapse. The maxillary could also be kept stable, and the mandibular had a little relapse.

The vocal cord polyp is easy to relapse after surgery, but if the patient has recurrence in a short term, it is necessary to consider it as postoperative vocal cord contact granuloma. If the patients with contact granuloma after surgical treatment had severe impact on the pronunciation, it is necessary to be operated and confirmed by pathology and given the treatment of acid suppression, in order to avoid postoperative recurrence.
Granuloma ; diagnosis ; Humans ; Otorhinolaryngologic Surgical Procedures ; adverse effects ; Polyps ; surgery ; Vocal Cords ; surgery