Objective To explore the effect of comfortable care in alleviating insomnia for cerebral infarction patients and improve the health of the patients. Methods Selecting 231 conscious and hospitalized cerebral infarction patients randomly from January to December,2005.Giving apprpfi-ate comfortable care measures in acute period,stable period and recovery period.Insomnia WaS evaluated at admission and after two weeks of hospitalization.Results Insomnia of cerebral infaretion patients allevi-ated after two weeks,which was statistically different from that at admission.P<0.01. Conclusions Comfortable care is significant to alhviate insomnia of cerebral infarction patients.reduce the com-plication and improve the quality of nursing service.We should emphasize it and put it into practice widely.

Objective To explore the clinical application value of serum procalcitonin (PCT) detection in diagnosis of neonatal infection disease.Methods The serum PCT,CRP values,routine blood test,blood cultures of 50 children with suspected infection was detected at admission and five days after admission.According to the clinical manifestations and the test results,50 children were divided into the bacterial infection group (32 cases) of and non-bacterial infection group (18 cases),and the value of PCT,CRP,routine blood test,blood culture was compared.Results Of bacterial infection group at admission,the serum PCT values in 27 cases were more than or equal with 2μg/L,white blood cell (WBC) increased in 6 cases,CRP of 7 cases were positive,blood cultures (3d) of 4 cases were positive,and of non-bacterial infection group at admission,the serum PCT value in 2 cases were more than or equal with 2μg/L,WBC increased in 2 cases,blood cultures (3d) of 1 case was positive ; Of bacterial infection group 5 days after admission,the serum PCT value in 23 cases were more than or equal with 2μg/L,WBC increased in 15 cases,CRP of 6 cases were positive,blood culture (5d) of 9 case were positive,and of non-bacterial infection group 5 days after admission,the serum PCT value in 1 cases was more than or equal with 2μg/L,WBC increased in 3 cases,CRP of 4 cases were positive,blood culture (5d) of 1 case was positive.At admission the cases of WBC increasing,CRP positive,blood culture positive of the two groups had no statistically significant differences (x2 =0.50,0.19,0.62,all P ＞ 0.05),but the cases of the PCT value which was more than or equal with 2μg/L had statistically significant differences between the two groups (x2 =25.30,P ＜ 0.05).5 days after admission the cases of WBC increasing,PCT,blood culture positive of the two groups had no statistically significant differences (x2 =4.39,20.30,8.56,all P ＜ 0.05),but the cases of the CRP value significant differences between the two groups (x2 =0.83,P ＞ 0.05).Conclusion The detection of serum PCT can be used as an important index of diagnosis for early neonatal infections,and its sensitivity and specificity are better than,blood routine and blood culture.

This study is to investigate the effect of recombinant hFGF-10 adenovirus on the proteome of HaCat cells, and to speculate further the possible mechanism of the effect of hFGF-10 on HaCat cells via differentially expressed proteins identified. Two-dimensional gel electrophoresis (2-DE) combined with tandem time-of-flight mass spectrometry was applied to identify the differentially expressed protein spots on the 2-DE maps of the whole-cell proteins from Ad infected and rAd-hFGF-10 infected HaCat cells. The mRNA and protein levels of the differentially expressed proteins were confirmed with semi-quantitative RT-PCR and Western blotting. The results showed that the 2-DE maps with high resolution were obtained, and four selected differentially expressed proteins involved in cell apoptosis, cytoskeleton regulation and protein degradation were identified with MALDI-TOF/TOF. The mRNA and protein levels of one of the differentially expressed proteins, VDAC2, were up-regulated in HaCat cells infected with the recombinant hFGF-10 adenovirus. The differentially expressed protein, VDAC2, may be related to the bioactivities of hFGF-10.

Objective To investigate the characteristics of pathogenic bacteria and drug resistance and susceptible factors of pulmonary infection in patients with severe brain injury and to provide a guideline for the prevention and control of infection.Methods Bacteria culture results combined with clinical data of pulmonary infection in patients with severe brain injury were analyzed retrospectively in our hospital from Jan.2004 to Dec.2008.Results The patients with severe brain injury were often complicated by pulmonary infection.Gramnegative bacilli were main pathogenic bacteria leading to pulmonary infection ( 74.8% ),including pseudomonas aeruginosa ( 21.0% ),Escherichia coli ( 16.5% ),baumanii ( 13.6% ),pneumobacillus ( 7.7% ),enterobacter cloacae (6.3% ) and others.Thirty-nine Gram-positive bacilli were observed including Staphylococcus anreus,coagulase negative staphylococcus,enterococcus and others.There were 21 fungus infection observed.Conclusions Overall and systematically monitoring the condition of pathogenic bacteria distribution and drug resistance of pulmonary infection in patients with severe brain injury have important significance to select antibiotics and effectively prevent and control pulmonary infection.

Epstein-Barr Virus (EBV) is the first human virus related to oncogenesis.EBV infection is associated with inflammatory bowel disease (IBD) with unknown causality.The prevalence of EBV in intestinal tissue from patients with IBD is significantly higher and related to the exacerbation of the disease and refractory IBD.Immunosuppressive therapy has improved outcomes associated with IBD.However,it is also associated with an increased risk of opportunistic infection,and lymphoproliferative disorders (LDs) maybe due to EBV infection.Here we review our current understanding of the pathogenesis of EBV infection in colonic mucosal inflammation,EBV-induced disease exacerbation,lymphomagenesis in IBD,and clinical approaches therefrom.

Crohn's disease is one of the main types of inflammatory bowel disease,some patients with Crohn's disease may be beyond the control of the medical complications,finally had to accept surgical treatment.This paper reviews the Crohng disease patients of surgical indications,gastrointestinal complications related to the classification,risk factors,prevention and treatment countermeasure research,designed for Crohn's disease indications for surgery decision and postoperative gastrointestinal complications in the diagnosis,perioperative treatment and prevention to provide some guidance and help.

EpiCalc is a capable tool for journal editors to review and send back manuscripts for revision due to its practical, simple and convenient advantages in verifying the data in scientific papers.Several examples of verifying the epidemiological data in scientific papers using EpiCalc were thus described in detail.

This study is to investigate the effect of recombinant hFGF-10 adenovirus on the proteome of HaCat cells, and to speculate further the possible mechanism of the effect of hFGF-10 on HaCat cells via differentially expressed proteins identified. Two-dimensional gel electrophoresis (2-DE) combined with tandem time-of-flight mass spectrometry was applied to identify the differentially expressed protein spots on the 2-DE maps of the whole-cell proteins from Ad infected and rAd-hFGF-10 infected HaCat cells. The mRNA and protein levels of the differentially expressed proteins were confirmed with semi-quantitative RT-PCR and Western blotting. The results showed that the 2-DE maps with high resolution were obtained, and four selected differentially expressed proteins involved in cell apoptosis, cytoskeleton regulation and protein degradation were identified with MALDI-TOF/TOF. The mRNA and protein levels of one of the differentially expressed proteins, VDAC2, were up-regulated in HaCat cells infected with the recombinant hFGF-10 adenovirus. The differentially expressed protein, VDAC2, may be related to the bioactivities of hFGF-10.

To examine the role of glycogen synthase kinase 3 (GSK-3) in the apoptosis of pancreatic beta-cells to better understand the pathogenesis and to find new approach to the treatment of type 2 diabetes, apoptosis was induced by oleic acid (OA) in INS-1 cells and the activity of GSK-3 was inhibited by LiCl. The PI staining and flow cytometry were employed for the evaluation of apoptosis. The phosphorylation level of GSK-3 was detected by Western blotting. The results showed that OA at 0.4 mmol/L could cause conspicuous apoptosis of INS-1 cells and the activity of GSK-3 was significantly increased. After the treatment with 24 mmol/L of LiCl, a inhibitor of GSK-3, the OA-induced apoptosis of INS-1 cells was lessened and the phosphorylation of GSK-3 was increased remarkably. It is concluded that GSK-3 activation plays an important role in OA-induced apoptosis in pancreatic beta-cells and inhibition of the GSK-3 activity can effectively protect INS-1 cells from the OA-induced apoptosis. Our study provides a new experimental basis and target for the clinical treatment of type-2 diabetes.
Apoptosis/*drug effects ; Cell Line ; Fatty Acids, Nonesterified/*pharmacology ; Glycogen Synthase Kinase 3/*metabolism ; Insulin-Secreting Cells/*cytology ; Oleic Acid/pharmacology ; Phosphorylation

0.05). Conclusion Both CD54 and LRP expression have negative correlation with the effective rate of short-term therapeutic effects, therefore can be taken as indexes for prognostic evaluation for patients with non-small cell lung cancer.