Secondary pancreatic infection in severe a-cute pancreatitis (SAP) was associated with mortality and the length of stay in hospital.Intensive Care Unit (ICU) identificate shock on account of the oxygen metaboilic level in earlier period of SAP and perform adequate fluid resuscita-tion.it advocate to perform organ function support and/or substitution therapy in time and think highly of coordination and combination each other among various kinds therapeutic measure.These therapeutic concept and measure will con-duce to decrease the incidence rate of MODS and secondary pancreatic infection accordingly in SAP.

Objective To observe effect of ganciclovir on the treatment of the young child viral encephalitis. Methods 68 cases of viral encephalitis were randomly divided into treatment group(45 cases) and the control group (23 cases), the two groups of children with clinical manifestations and treatment effects were observed. Results Headache, vomiting, convulsions symptoms and the resumption of the time was obviously shorter in treatment group than that of the control group, fever symptoms persisted despite the shortened time, but the difference was not statis- tically significant. Conclusion Ganciclovir in children with viral encephalitis have significant effect.

Objective To study the trends of injury mortality rates among residents in Dafeng city from 1976 to 2006, and provide a scientific basis for preventing and controlling injure. Methods The death registry data from 1976 to 2006 in Dafeng city was collected and its injury death trends was analyzed with joinpoint regression method and the relationships of injury mortality rates with sex, age and time were analyzed with Poisson regression method. Results A total of 13905 cases died from injuries in Dafeng city from 1976 to 2006. The gross injury death rate was 61.40 per 100 000, 70.18 per 100 000 in male and 51.85 per 100 000 in female (P

PURPOSE To investigate the effects of bcr3/abl2 anisense oligodeoxynucleotide (ASO) on chronic myeloid leukemia (CML). METHODS The effects of bcr3/abl2 ASO in 15 CML patients were evaluated by morphological observation, flow cytometer (FCM) techniques and DNA fragment measurement. RESULTS bcr3/abl2 ASO can induce apoptosis in CML. CONCLUSION Antisense techniques may provide a new way to cure CML.

Objective Preparation of specific antibody of neuron protein 14 3 3 and application for the diagnosis of prion diseases. Methods The rabbits were immunized with prokaryotic expressed GST 14 3 3 fusion protein and the collected antiserum was evaluated by ELISA and Western blot assays. Results ELISA revealed that the reactive titer of the prepared antibody against purified 14 3 3 protein was up to 1:4 096 000; Western blot assays confirmed that both prokaryotic expressed,and the natively presenting 14 3 3 proteins in the brain extracts were recognized by the prepared antibody. Two cases of clinically diagnosed Creutzfeldt Jakob disease (CJD)with typical EEG changes showed the positive 14 3 3 protein in cerebral spinal fluid (CSF). Four patients with dementia and narcoma but without typical EEG change were 14 3 3 negative in CSF,three of them being even further tested by brain biopsies without typical histological change of CJD and presentation of protease resistant PrP Sc protein. 35 samples CFS collected from patients without dementia were 14 3 3 negative in CSF. All the results were the same as that of standard 14 3 3 polyclonal antibody.Conclusion Using purified prokaryotic expressed GST 14 3 3 fusion protein as antigen,the specific antibody was elicited in the immunized animals. The prepared antibody can be used in identification of 14 3 3 protein in CSF for the diagnosis of CJD.

At present, the immunological tolerance in transplantation induced by multi-mechanisms, to inhibit the chronic graft rejection, represents an important trend in the field of transplantation. This review summarizes the new researches about immune and non-immune factors in this field. At the same time, this review also discusses that the high-dose vitamin E, through changing the ratio of Th/Ts in vivo and not improving the level of specific-antibody, regulates the immune system. In addition, the potent antioxidation of vitamin E has advantage to release the oxidate press of tissue and the damage of blood vessels. In the end, the reporter prospects the signification of vitamin E to inhibit the chronic graft rejection and points out the potential questions.

Objective To study the clinical,endoscopic an d pathologic features of primary gastric malignant lymphoma,and to improve its diagnostic efficiency.Methods The clinical,pathologic and endoscopic data in twenty two cases of primary gastric malignant lymphoma were retrospectively reviewed. Results In these cases,the common presentations include abdomi nal pain、anorexia、loss of weight etc. These patients were all examined by endo scopy with protruding,ulcerative and infiltrative lesions. Submucosal lesions w ith hypo echoes were found by endoscopic ultrasonography in six cases,and enlar ged lymph nodes were found on the exterior of stomach in two cases. Conclusion The primary gastric malignant lymphoma has a rather low incidence,and without specific clinical manifestations, hard to differentia te gastric ulcer from cancer through the manifestation in endoscopy. Further com bination of endoscopy, endoscopic ultrasonography and pathology are helpful to i ts correct diagnosis.

Objective To investigate the outcomes of arthroscopic treatment of anterior cruciate ligament tibial eminence avul-sion fractures by using loop ligature fixation .Methods 26 patients with anterior cruciate ligament tibial eminence avulsion frac-tures .All the patients were fellowed up at least one year .Results The X-ray indicated that all the avulsion fractures of tibial anteri-or eminence were healed after 6 months of the operation .The mean Lysholm score improved from (50 .8 ± 6 .5) preoperatively to (92 .7 ± 4 .3)postoperatively(t=36 .7 ,P<0 .05) .The mean IKDC 2000 subject score improved from (55 .6 ± 7 .4)preoperatively to (90 .7 ± 4 .2) postoperatively(t=22 .5 ,P<0 .05) .Conclusion Arthroscopic treatment of anterior cruciate ligament tibial eminence avulsion fractures by using loop ligature fixation could provide satisfying reduction ,stable fixation and well healing of the avulsed fragment .This arthroscopic suture fixation method is a minimally invasive ,simple and effective treatment for patients with tibial eminence fracture .

Objective To evaluate the role of oxidative stress in the spinal neurotoxicity induced by ropivacaine in rats.Methods Ninety pathogen-free male Sprague-Dawley rats,weighing 250-320 g,aged 3 months,in which intrathecal catheter was successfully implanted into L5,6 interspace without complications,were randomly divided into 3 groups (n =30 each) using a random number table:control group (group C),ropivacaine group (group R),and antioxidant Tempol group (group T).The rats received 1％ ropivacaine 1.2 μg/g for 8 times at 1.5 h intervals through the catheter in R and T groups,while the rats received the equal volume of normal saline instead in group C.In T group,Tempol 20 μg/g was injected intrathecally at 4,8,12,24,48 and 72 h after the last injection of ropivacaine.The rats were sacrificed at 1,3,5,7 and 14 days after the end of ropivacaine injection,and their lumbar enlargements were removed for TUNEL staining to detect the cell apoptosis.SOD activity was determined by colorimetry and MDA content was measured using TBA photoelectric colorimetry.Apoptosis index was calculated.Results Compared with group C,apoptosis index and MDA content were significantly increased,and SOD activity was decreased in R and T groups.Compared with group R,apoptosis index and MDA content were significantly decreased,and SOD activity was increased in group T.Conclusion Oxidative stress is involved in the development of spinal neurotoxicity induced by ropivacaine in rats.

Objective:To study memory changes in patients with non-demented Parkinson disease(PD) without depression.Methods:The Nissen Version(serial reaction time task,SRTT) software(as a task of procedural learning),the WMS-CR and two tasks of implicit memory were applied in 16 PD patients(Hoehn-Yahr score I~Ⅱdegrees).Normal controls enrolled for the Nissen Version either.Results In the explicit WMS-CR and the implicit(word stem completion and degraded picture naming) tasks,the patients' scores fell within normal limits(Memory Quotient 97.1?10.6).In the SRTT,normal control group displayed significantly reduced response times(F=2.54,P=0.008) and error rates(3.2 ?0.9% to 6.8 ?2.7%,t=-2.08,P=0.045) across the blocks of repeated sequence trials.By contrast,PD patients only showed a reduction in error rates(4.7?2.0% to 3.7?1.8%,t=-2.15,P=0.038) but no change in response times.Conclusion:Impairment of nigrostriatal pathways selectively affects performance in visuo-motor learning tasks such as the SRTT,but not in the explicit tasks of WMS-CR and the implicit tasks such as word stem completion and degraded picture naming.