Objective To investigate the lentivirus-mediated RNA interference(RNAi) reduced human epidermal growth factor 2 (HER2) gene expression in ovarian cancer chemosensitivity.Methods Specifically targeted short hairpin RNA HER2 gene ( shRNA) lentiviral expression vector and negative control sequences lentiviral vector were constructed.stable expression of HER2-shRNA in SKOV3 cell line ( KD) and negative control cell line (NC) were established.The uninfected SKOV3cell lineserved as blank control(CON)group, sensitivity to changes in three kinds of cells to cisplatin chemotherapy in the HER2 gene expression was detected by RT-PCR and Western blot technique, CCK-8 assay CON and KD cells strains were established xenograft model in nude mice in each group to detect the expression of the HER2 protein, as well as by cisplatin chemotherapy tumor size. Results Expression results showed that a stable cell line HER2-shRNA and negative control cell lines had been successfully establish by fluorescence and flow cytometry.RT-PCR results showed KD cells relative HER2 mRNA expression levels compared with the NC group, the difference was statistically significant (P<0.01), whereas no significant difference when compared with the CON group, HER2 mRNA KD Group inhibition rate was (95.5 ± 0.12)%.After transduction specific HER2-shRNA ovarian cancer tumor cells, HER2 protein expression was significantly reduced, growth had slowed down significantly after cisplatin treatment ( P<0.01 ) .Conclusion Lentivirus-mediated HER2-shRNA can effectively inhibit the expression of the HER2 gene, increase chemosensitivity to cisplatin in ovarian cancer.

Objective To observe the efficacy of ganglion glycosides in the treatment of elderly patients with Parkinson's disease and its influence on the serum tumor necrosis factor -alpha(TNF -α).Methods 60 elderly patients with Parkinson's disease were selected,and according to the different treatments,they were randomly divided into the observation group(n =30)and control group(n =30).The control group was given routine treatment,and the observation group was given ganglion glycosides treatment on the basis of routine treatment.The comprehensive rating scale score changes were observed,and the curative effect,serum TNF -αlevel changes were compared between two groups.Results The total effective rate of the observation group was 53.3%,which was higher than 23.3% of the control group(χ2 =6.721,P <0.05).After treatment,Parkinson's disease rating scale score of the indexes (spirit, behavior,emotions and daily activities and motor function)of the two groups were significantly lower(t =3.571, 5.726,7.153,all P <0.05).The serum TNF -αconcentrations before and after treatment of the observation group were (27.4 ±6.1)μg/L,(13.1 ±3.4)μg/L,respectively,and those of the control group were (27.6 ±6.0)μg/L, (20.3 ±4.7)μg/L respectively.After treatment,the serum TNF -αlevels of the two groups were significantly lower than before treatment (t =5.614,8.352,all P <0.05 ),and after treatment,the serum TNF -α level of the observation group was significantly lower than that of the control group (t =4.241,P <0.05).Conclusion Ganglion glycosides in the treatment of elderly patients with Parkinson's disease can make slow markedly improved,lower levels of serum TNF -α,has significant clinical therapeutic effect.

Objective To provide an additional reference and value for the surgeon to reconstruct the natural and aesthetic breast. Methods Measurements were made in a group of 45 women volunteers aged from 22 to 45 years, who were satisfied with their breasts without any history of breast surgery. After the lateral arch of the breast as the reference value for breast size being chosen, breast-areola to nipple ratio was determined according to parameters measured as follows: weight, height, diameter of the breast, areola, and nipple, distances between the nipple and borders of the breast-superior (to the suprasternal notch), medial arch (to the medial end of the inframammary crease), lateral arch (to the lateral end of the inframammary crease), inferior arch (to the lowest point of the inframammary crease). Correlation factors such as age, weight, height and body mass index (BMI) which may influence the proportion were also considered and analyzed. Results The sample showed that the breast- areola and areola-nipple proportions were about 3.7:1.0 and 3.4:1.0 respectively in the Chinese women population. Weight/BMI had a significant positive correlation with the breast size and areola-nipple proportion, nor did age and height in the sample. Conclusions Breast and areola-nipple complex keeps their optimal proportions, which might be influenced by the factor of overweight. Determination of breast-areola-nipple proportion contributes its aesthetic value to the mammaplasty and reconstruction for the oriental female. The proportion could be used to predict and design the desired breast size during the operation.

Objective To evaluate the efficacy and safety of specific immunotherapy (SIT) to allergic rhinitis in children.Methods Sixty-four patients with mite allergy allergic rhinitis in children,were divided into two groups by random digits table:treatment group and control group,each group with 32 cases.Treatment group was given SIT with standardized allergen vaccine for 3 years on the basis of symptomatic therapy,control group only received symptomatic therapy.Observation indexs included rhinitis symptoms score,drug score,skin index (SI),serum specificity IgE (sIgE),peripheral eosinophil (Eos) counting,development of asthma and the new sensitization.Results The Eos counting,SI after treatment 3 years in treatment group were significantly better than those before treatment and those in control group after treatment 3 years[(0.14 ± 0.12) × 109/L vs.(0.74 ± 0.18) × 109/L,(0.78 ± 0.36) × 109/L and 1.03 ± 0.13 vs.1.51 ± 0.32,1.51 ± 0.37] (P ＜ 0.01).There was no statistically significant difference in sIgE between two groups (P ＞0.05).The rhinitis symptoms score,drug score in two groups after treatment 1,2,3 years were significantly better than those before treatment (P ＜ 0.01).The rhinitis symptoms score,drug score in treatment group after treatment 1,2,3 years were significantly better than those in control group(P ＜ 0.01).The rate of new sensitization in treatment group was significantly lower than that in control group [3.1％ (1/32)vs.34.4％ (11/32),P ＜ 0.01].Conclusion Keeping long-term SIT is effective and safe for children's allergic rhinitis induced by mite,it also prevents new allergen appeared and allergic rhinitis development for asthma.

Objective To analyze and evaluate the impact of trusteeship on hospital management and operating status. Methods The state of affairs, technical merit and financial situation and other datawere invested and analyzed in some trust hospital in Xinjiang from 2007 to 2010. Results The number of outpatients, inpatients and surgeries compared before hosting increased by 30. 3%, 61.3% and 26. 3%, respectively. The average in hospital shortened 1.1 days. The growing medical expenditure of average outpatientand inpatient was controlled at a reasonable range. 33 new technologies were carried out after trusteeship. The total revenue in 2010 increased 54.2% compared to 2007, with the balance between revenue and expenditure. Conclusion Hospital trusteeship helped improve hospital operation and management. Measures for hospital trusteeship should be suited to local conditions and sensible.

Objective To present a new method of osteotomy for malar complex hypertrophy .Methods The operative procedures was transpositional osteoplasty of the malar complex by coronal approach. A lateral orbital osteotomy began at the level of the frontozygomatic suture line,preserving the lateral orbital rim. It continued far down inferoposteriorly across the zygomaticomaxillary suture line. The zygomatic arch was obliquely cut at 1 cm anterior from the articular tubercle. The bone was not separated from the soft tissue to preserve the blood supply to the zygoma and slid upward, inside and setback. The single microplate,screw and steelwire fixation was done on the repositioned zygomatic body.To zygomatic body prominence,it was obtained a satisfactory effect that only shaved the protruded zygomatic body with chisels by means of an intraoral approach.Operative procedures for reductive malar complex plasty were performed in 12 cases. The patients consisted of 3 males and 9 females whose ages ranged from 21 to 36 years.Results Postoperative follow up for 3～24 months showed satisfactory results using this procedure.Conclusion This modified method has an ideal effect without the possibility of bone resorption of the malar complex.

Objective To investigate the clinical characteristics and differences among Uygur and Han patients with simple severe preeclampsia and their first-degree relatives. Methods To analysis the 100 cases with simple severe preeclampsia among Uygur and Han patients and their first-degree relatives. Results The age and gestational age between Uygur and Han group, there was no significant difference (P>0. 05). Average production time, women with preeclampsia history and BMI of Uygur group were higher than the Han group(P < 0. 05). Antenatal examination times of Uygur group was lower than the Han group(P < 0. 05). Hypertension, cardiovascular and cerebrovascular disease prevalence rate of mother in the Uygur group were higher than their father(P < 0. 05). The comparison of parents in Han and Uygur group were no statistically significant difference (P>0. 05). Conclusion During pregnancy, the Uygur should pay more attention to improve diet structure, control body weight, and strengthen their pregnancy health care, so that the disease can be early diagnosed and treated, reducing or avoiding therapeutic preterm labor.

Electret is a kind of functional material which can store charge for a long time. The external electrical field and microcurrent produced by electret can be applied to restore the electret state of the injured tissue, to improve the microcirculation and to accelerate the union of fracture, wound and nerve regeneration. Recently, the application study of electret in life science has been proceeding briskly. This paper briefly summarizes the application situation of electret in life science.

In order to consummate medical emergency equipment in biochemistry terror incident for Chinese People's Armed Police Forces,a set of emergency succor equipment is developed,which processes such function as biochemical detection,biochemical wash & antisepsis,biochemical treatment & cure and biochemical prevention.Its flexibility and practicability are stood out so as to upgrade guarantee ability in biochemistry terror incident.