Objectives　To study of the method for isolation and transplantation of mouse islets. Methods　The method for isolation of mouse islets described by Gotoh G was modified. The solution of digestion was injected not through the common bile duct but through gallbladder. Soybean trypsin inhibitor and BSA were added into the digestive and Ficoll separation solutions. Results　The yield of islets was increased from 41.7±13.2 to 266.5±32.1(P<0.01). Islet viability was more than 95!%. Among the purified islets, there was no exocrine tissue but few ductal fragments. Conclusions　By the improved method, digestive solution could be injected into pancreas without inverted microscope, which made manipulation easier and more successful. Having avoided the digestive effect of trypsin on islets, the yield of islets was increased and good repetitiousness was obtained.

The active form of vitamin D,1,25-Dihydroxyvitamin D3 \,is a secosteroid hormone that binds to the vitamin D receptor(VDR),a member of the superfamily of nuclear receptors for steroid hormones,thyroid hormone,and retinoic acid.1,25-Dihydroxyvitamin D3 and its analogue regulate calcium and bone metabolism,control cell proliferation and differentiation,and exert immunoregulatory activities.Recent advances in understanding their functions and novel insights into the immunomodulatory mechanisms they control suggest a wider applicability in the treatment of autoimmune diseases and induction of allograft tolerance.In addition to direct effects on T cell activation,1,25-Dihydroxyvitamin D3 and its analogue modulate with different mechanisms the phenotype and function of antigen-presenting cells(APC),and,in particular,of dendritic cells(DC).In vitro and in vivo experiments have shown that 1,25-Dihydroxyvitamin D3 and its analogue induce DC to acquire tolerogenic properties that favor the induction of regulatory rather than effector T cells.These intriguing actions of 1,25-Dihydroxyvitamin D3 and its nanlogue have been demonstrated in several experimental models and could be exploited,in principle,to treat a variety of human autoimmune diseases,or inhibit allograft rejection.

1,25-dihydroxyvitamin D3,the activated form of vitamin D, is a secosteroid hormone.In addition to its central function in calcium and bone metabolism,1,25-dihydroxyvitamin D3 has important effects on the growth and differentiation of immune system.1,25-dihydroxyvitamin D3 interacts with its specific receptor,the vitamin D3 receptor(VDR).APCs and T cells can be direct targets of the hormone,leading to the inhibition of pathogenic effector T cells and enhancing the frequency of T cells with regulatory properties, largely via induction of tolerogenic DCs.1,25-dihydroxyvitamin D3 and its analogs have been shown to inhibit autoimmune diseases and graft rejection in several experimental models.It is a complicated and fertile area of investigation. By the investigation may important new therapies emerge for autoimmune disease and graft rejection.

Objective To study the clinical features of primary signet ring cell carcinoma of prostate. Methods 2 cases of primary signet ring cell carcinoma of the prostate were studied and reviewed. Results The age of the 2 patients was 64 and 73.The clinical symptoms were dysuria,vesical irritability and perineum discomfort. Histologically, signet ring cell carcinoma was composed of round cells with abundant-clear cytoplasm and crescent-shaped nuclei on one side.Mitosis were frequently observed.Immunohistochemical testing showed the cancer cell was positive for prostate specific antigen (PSA),prostate acid phosphatase (PAP),AR,cytokeratin and negative for carcinoembryonic antigen (CEA),alcian blue/periodic acid-schiff (AB/PAS).One case (stage D)died 6 months after bilateral orchiectomy and flutamide therapy because of wide-spead metastasis;the other (stage B 2) has been surviving 25 months after radical prostatectomy,bilateral orchiectomy,endocrine therapy and local irradiation ministration. Conclusions Primary signet ring cell carcinoma of the prostate is extremely rare with a high grade of malignancy. The diagnosis depends on histopathologic and immunohistochemical studies.

Glucose-6-Phosphatase (G6Pase) was regarded as a marker enzyme of the endoplasmic reticulum in a number of different cells. The purpose of this report is to study the localization of G6Pase activity in the rat left ventricular myocardial cells. G6Pase activity was found in the lumen of the nuclear envelope, the sarcoplasmic reticulum(SR) and the subsarcolemmal cisterns. The SR tubules between the adjacent myofibrils displayed characteristic distribution on their longitudinal profiles, as a curtain-like network, the tubules appeared to be tight network facing A-band, whereas tubules formed large polygonal meshes facing I-band. It is thought that the SR tubules facing A- and I-bands, respectively, represented an adaptation of SR to the selective shortening of the myofibrils at the I-band during contraction.

Left ventricular myocardial hypertrophy was produced in the rats by ligation of the abdominal aorta below the diaphragm for seven weeks.Ultrastructurally, it was observed that the nucleus and nucleolus were enlarged, and the density of the chromatin of the hypertrophied myocardial cells was decreased. Free ribosomes and endoplasmic reticulum were increased. Golgi apparatus was well developed and was increased in number.Cytochemically, G6Pase activity was localized in the lumen of the sarcoplasmic reticulum, nuclear envelope and subsarcolemmal cisterns, and it was also positive in the regenerative rough endoplasmic reticulum. TPPase activity appeared in the Golgi apparatus, and it was especially prominent in the Golgi apparatus of the hypertrophied cells.These findings suggest that the protein synthetic activity was increased in the hypertrophied myocardial cells.

Objectives:The effects of human growth hormone (hGH) on cell cycle kinetics were studied in human colonic cancer cell line, LS-174-T cell.　Methods: The cells were cultured in RPMI－1640 medium for 24 hours with hGH at concerntration of 50, 100, 200 ng/ml with or without cisplatin. Cell cycle kinetics was analyzed by flow cytometry.　Results: The significant effects of hGH on cell cycle kinetics were found in the cell line. Percentage of S phase increase (P<0.01) and percentage of G2/M phase decreased (P<0.01).DNA Index (DI) didn't increase.The cells had a lot of apoptosis and percentage of S phase decreased (P<0.01) with addition of cisplatin.　Conclusions: In vitro hGH may induce the proliferation of colonic cancer cells. Cisplatin may suppress the stimulatory effects of hGH on S phase and increase the apoptosis of colonic cancer cells.

CdSe/ZnSquantumdots(QDs)werepreparedandcovalentlylinkedtoanti-katacalcinmonoclonal antibodies. After modification, the QDs' maximum emission wavelength was shifted to 625 nm from 620 nm while maintaining the spectral properties. Then the QDs labeled lateral flow strip and corresponding fluorescence measuring instrument were designed and fabricated. To reduce the cost of strip by reducing the amounts of monoclonal antibodies, appropriate amounts of QDs labeled monoclonal antibodies were sprayed on the conjugation pad, with just one test line on the strip but without the control line. Parameters of the strip were optimized by measuring the signal to noise ratio. By using the strip and fluorescence measuring instrument, procalcitonin (PCT) could be detected in 20 min, and the quantitative detection range was 0. 2-100 μg/L with sensitivity of 0. 1 μg/L. A total of 22 blood samples were measured by both our method and the commercial instrument used in the hoptital. The results were consistent for their Pearson correlation coefficient (0. 9995) and Kolmogorov-Smirnov test (Sig=1. 0). The rapid quantitative detection method for PCT is of great importance to quantitative detection of bacterial infection and rational usage of antibiotics clinically.

Objective To study the immunomodulative effects of 1,25-Dihydroxyvitamin D_3 1,25-(OH)_2D_3 on the predominant Th1 response rats. Methods Lewis rats had been fed with 1,25-(OH)_2D_3 (1000ng per rat) for 14 days. The rats in control group were administered vehicle with the same volume as the drug to serve as the experimental group, and then LPS (10ng/kg) was administered intraperitoneally in both groups on the 15th day. 15 rats of each group were sacrificed 6h later and immunological index was measured. Mortality of the remaining ten rats in both group have been observed. Results There was no death in experimental group, while five control rats died at 24h (5/10, 50%), and all the other five control rats survived at 96h. IL-12 and IFN-? were decreased significantly in the experiment group compared with control (3986?328pg/ml vs 4160?289pg/ml, P=0.028; 4840?802pg/ml vs 5264?524pg/ml, P=0.020), while IL-4 was increased (5.57?1.75pg/ml vs 3.72?1.62pg/ml, P=0.036). In white pulp of the spleen of experimental rat the number of lymphocytes decreased significantly as shown with HE-staining, and the PCNA positive cells were distributed focally or dispersively. In addition, by using flow cytometer, it was found that CD4~+ CD25~+ positive lymphocytes were increased significantly in the spleen of experimental rats (1.09%?0.29% vs 0.73%?0.00%, P

Objective To study the changes of the semen quality and the spermatogoa ultrastructure in uremic patients before and after renal transplantation. Methods The semen was analyzed and spermatogoa were investigated under scanning electron microscopy (SEM) in 8 patients before and after renal transplantation and 5 normal volunteers. Results In uremic patients, there was significant decreases in sperm motility, survival rate and sperm density. Under SEM, the deletion of the acrosome, distinct vacuoligation in the nucleus, deficiency of the postacromal and postnuclear ring and ~absence of the middle segment of mitochondrial sheath were found. After renal transplantation, the semen main parameters were significantly improved (P