Objective To investigate the protective effects of Lycopene (LP) on hepatic oxidative stress injury in type 2 diabetic rats.Methods The diabetic rat models were setup by intraperitoneal injecting STZ (60 mg/kg). 60 models were selected and randomly divided into three groups: diabetes model control group, LP 10 and 20 mg/kg treated groups, with 20 experimental animals in each group. While, selected another 20 normal same-aged rats as normal control group. The drug was given by intragastric administration for 4 weeks, once a day. Before the drug was given and after 2, 4 weeks, the level of blood sugar was determined. Four weeks later, the body weight and hepatic index were detected; the activity of ALT, AST, ALP and the level of T-AOC in serum were determined; the activity of SOD, GSH-Px, CAT and the content of MDA in hepatic tissue were determined; and the hepatic tissue histopathological changes was observed by HE staining.Results Compared with the diabetes model group, the activity of ALT in serum of LP 10, 20 mg/kg treated groups was significantly decreased (714.8±291.5 U/L, 862.7±348.1 U/Lvs. 1 007.6±375.7 U/L); AST was significantly decreased (75.2±18.6 U/L, 87.6±20.3 U/L vs. 99.3±21.5 U/L); and the content of MDA in hepatic tissue was significantly decreased (5.56±1.02 U/L, 5.83±0.98 U/L vs. 6.37±1.18 U/L); the blood sugar level of LP  20 mg/kg treated group was significantly decreased(16.6±1.1 mmol/L vs. 17.9±1.2 mmol/L), the body weight was significantly increased(281.5±24.8 g vs. 252.3±23.5g) and the hepatic index was significantly decreased (26.2±5.3 ‰vs. 30.4±5.8‰), the activity of ALP in serum was significantly decreased (135.2±30.8 U/L vs. 167.4±32.5 U/L) and the level of T-AOC was significantly increased (10.8±2.8 U/L vs. 8.7±2.4 U/L), the activity of SOD in hepatic tissue was significantly increased(10.8±2.3 U/mgvs. 9.1±2.3 U/mg), GSH-Px was significantly increased (16.1±3.4 U/mgvs. 14.3±3.5 U/mg), CAT was significantly increased (3.16± 1.08 U/mgvs. 2.63±0.90 U/mg).ConclusionLP had dose-dependent protective effect for hepatic oxidative stress injury in type 2 diabetic rats.

Despite the general agreement that calculation ability is an important component of cognition,and a comprehensive cognitive evaluation should include testing for calculation ability,there is a significant paucity in study and evaluation of acalculia.In this paper,the concepts and classification of acalculia are proposed;main neuropsychological theoretical models and cerebral mechanisms are presented;clinical appearances,standard testing instruments and related study of acalculia are intensively reviewed;finally,rehabilitation training of acalculia is initially discussed.

Objective To investigate errors and mechanism of acalculia in patients with brain injury.Methods Forty patients with brain injury and forty-eight normal adults who were matched in age and years of education were tested with EC301 standardized battery. Patients were divided into the left-brain damaged (LBD) group (n=20), right-brain damaged (RBD) group (n=9) and bilateral brain damaged (BBD) group (n=11). The data of all subjects were statistical analyzed.Results Both total scores and scores of 27 out of 31 items were remarkable lower in patients than those in the normal control group ( P<0.05). The total scores and items' scores for number sequences, numerical transcodings were significantly lower in LBD group than those in RBD group ( P<0.05). Total scores and each items' score did not significantly differ between BBD group and LBD group, or between BBD group and RBD group ( P>0.05).Conclusion The abilities of number processing and calculation are impaired in patients with brain injury. There is either association or dissociation in terms of performance of acalculia and aphasia in LBD patients. Visuospatial impairment is related to performance of acalculia in RBD patients.

BACKGROUND: Differences of thick and thin tissues in imaging areas is great because of postero-anterior images in cervical spine-thoracic spine.Although there are many image processing functions in direct digital radiography(DR)image with big range dynamic exposing,but routine direct DR hardly reveals anatomic structure of cervical spine-thoracic spine to detect diseases.Image quality of cervical spine-thoracic spine requires improvement.OBJECTIVE: To evaluate the difference between direct DR image with tissue equalization(TE)technique and standard DR in cervical spine-thoracic spine,so as to explore the best image quality in cervical spine-thoracic spine.METHODS: A total of 200 cases were randomly selected from DR cervical thoracic image from Department of Radiology,General Hospital of Chinese PLA between April 2005 and August 2009 to evaluate the difference between these images of DR processed by tissue equalization and that of standard DR.RESULTS AND CONCLUSION: The detail images of DR with tissue equalization in different thickness area of body could be revealed clearly in the same image,while the detail images of standard DR in cervical thoracic could be revealed clearly by adjusting window width and location repeatedly.Results show that the image quality of TE technique of direct digital X-ray radiography in cervical spine-thoracic spine could be clearly improved,it could be easily read and avoid the influence of body thickness area.Moreover,it could reveal clearly other part of body in detail.DR image with TE technique in cervical spine-thoracic spine can be applied widely due to simple operation,high diagnosis rate and low cost.

BACKGROUND:The cervical pedicle screws mistakenly inverted the cervical pedicle could lead to the many complications such as spinal cord,vertebral arteries and nerve root damage.If the parameter of inserting pedicle screws were measured accuracy,the rate of inserting pedicle screws successfully could be improved.OBJECTIVE:To get 3D anatomic parameter of inserting pedicle screws in the cervical vertebrae with the CT three-dimensional recontruction.METHODS:The Dicom data that the cervical vertebrae had been scanned by SIEMENS EMOTION 16 CT were converted into the soft Mimics 8.1,GE workstation and three-dimensional cervical vertebrae were reconstructed.The parameters of inserting cervical pedicle screws were measured with the consideration of the diameter of pedicle screw.RESULTS AND CONCLUSION:The cervical three-dimension images were reconstructed clearly with the CT three-dimensional reconstruction techniques.The cervical pedicular width,the axial length,α and β angles were measured by the soft measure function.The average inserting angular safe range of α angle was gained with the consideration of the diameter of pedicle screw.This method can mimic the operation and it is significant way in measuring the three-dimension anatomic parameter of the cervical vertebrae.The method is a good way that how to insert the cervical pedicle screw safely.

Objective To study medical fear and its influencing factors in hospitalized school-age children in Lanzhou area.Methods Children's Medical Fear Scale was used to investigate 110 cases of hospitalized school-age children,and the investigation result underwent analysis.Results The total score of children's medical fears was (50.43±7.34),the three items with the highest score among 30 items were surgery,death,being deprived of education,homework delay,surgery and death tied for first.Conclusions Medical fear of school-age children was related with age and gender,female was significantly higher than that of male.No relationship with the family education environment,duration of illness,area of residence.

Objective: To research the best processing method for ginger pinellia by orthogonal tests.Methods: The orthogonal tests included the soaking time, boiling water and cooking time as the influencing factors, an HPLC method was used for the determination of 4 nucleosides (uridine, guanosine, adenosine, inosine), and the alum limit and extract content were also studied.The results were evaluated by multi index comprehensive weighted score to optimize the processing technology of ginger pinellia.Results: The best processing technology of ginger pinellia was as follows: soaked for 60 hours, the proportion of boiling water and pinellia tuber was 15:1, and boiled for about 5 h.Conclusion: The optimum processing technology of ginger pinellia is reasonable, reliable and reproducible, which can be used as the reference for the processing standardization of Chinese crude drugs.

The article describes the development of an Internet-based tele-rehabilitation system for patients with cognitive deficits,including overall idea,design and structure of the system,role-based authorization of operations for the patients and doctors and the procedures for patients' receiving rehabilitation treatment through the system.It is a new and convenient mode for rehabilitation service delivery to patients with cognitive deficits.Implementation of the Internet-based tele-cognitive rehabilitation system is helpful to the patients who live far from hospitals and have difficulty seeing doctors due to physical impairments,and is helpful to solving the problem with lack of medical staffs specialized in cognitive rehabilitation.Furthermore,it will also profit pushing out the spread and development of cognitive rehabilitation in China.

OBJECTIVETo study the activation of platelets in children with Kawasaki disease (KD) at molecular level.

METHODSThe expression of platelet surface glycoproteins CD(41), CD(42a), CD(61), CD(62p) and CD(63) in 20 KD patients was measured by flow cytometry before and at 1, 2, 3 week after treatment with aspirin and high-dose (1 approximately 2 g/kg) intravenous gamma-globulin (IVIG).

RESULTSThe expression of glycoprotein CD(41), CD(42a), CD(61), CD(62p) and CD(63) were higher in KD group than in control group. Aspirin and IVIG could not inhibit these high expression of glycoproteins. Higher expression of CD(62p) was observed in patients with coronary artery injury.

CONCLUSIONPlatelets were highly activated in KD patients which may be one of the most important pathophysiological step in KD. It provided a theoretical basis for treatment of KD with antagonist of glycoprotein of platelets. Obviously increase of CD(62p) can be taken as a criterion for predicting coronary artery injury in KD patients.

Aspirin ; pharmacology ; Blood Platelets ; drug effects ; metabolism ; Child ; Child, Preschool ; Coronary Artery Disease ; complications ; metabolism ; Female ; Humans ; Injections, Intravenous ; Male ; Mucocutaneous Lymph Node Syndrome ; metabolism ; pathology ; P-Selectin ; metabolism ; Platelet Activation ; physiology ; Platelet Membrane Glycoproteins ; metabolism ; gamma-Globulins ; pharmacology

Objective To investigate the safety and efficacy of endoscopic subserosal dissection (ESSD)for patients with upper gastrointestinal submucosal tumors. Methods A retrospective study was performed on the data of 6 patients with submucosal tumor undergoing ESSD in Nanjing Drum Tower Hospital from October 2016 to February 2017. The surgery time, surgical success rate, postoperative pathology and complications were analyzed. Results All patients successfully completed surgery. One patient had serosal perforation. The mean diameter of tumor was 27.5±10.0 mm. The mean surgery time was 49±18 min. The postoperative pathology of lesions was very low risk stromal tumor. No serious complications occurred. Conclusion ESSD is safe and reliable for patients with tumor originated from muscularis propria.