ObjectiveTo explore the influence of different hemostatic measures of laparoscopic ovarian cyst removed on ovarian function.MethodsSeventy patients with ovarian cyst who performed laparoscopic ovarian cysts removed between January 2009 and December 2010 were randomly divided into group Ⅰ (23 cases) and group Ⅱ (47 cases) by mechanical sampling.Group Ⅰ with bleeding electrocautery,group Ⅱ with suture.Estradiol,follicle puncture hormone (FSH),luteinizing hormone (LH),number of sinusoidal follicles,normal ovarian function and the number of declining ovarian reserve were compared in two groups before operation and after 6 months operation.ResultsAfter operation,the proportion of group Ⅱ with normal ovarian function was significantly higher than that of group Ⅰ[80.9％(38/47) vs.39.1％(9/23),P ＜ 0.05 ],estradiol and number of sinusoidal follicles were significantly higher than those of group Ⅰ [( 198.4 ± 24.4) pmol/L vs.( 153.4 ± 16.8) pmol/L,7.1 ± 2.4 vs.5.8 ± 0.9](P＜ 0.05),FSH and FSH/LH were significantly lower than those of group Ⅰ[ ( 10.58 ± 4.57) U/L vs. ( 13.72 ± 2.58) U/L,2.34 ± 1.32 vs.2.82 ± 0.88 ] (P ＜ 0.05).ConclusionDuring laparoscopic ovarian cyst removed,using electrocautery can significantly reduce residual ovarian function,but using suture hemostasis can effectively protect residualovarian function.

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Post-ERCP pancreatitis(PEP) is the most common and serious complications about endoscopic retrograde cholangiopancreatography(ERCP).A number of studies have been confirmed that PEP can be prevented by pancreatic stents.We summarized earlier results in this field to expand the clinical use for this high-tech.

Objective By contrasting and analyzing the clinic manifestations, radiological features and pathological results of patients with bone invasion from skin of the lower limbs that were associated with malignancy, authors advanced X-ray diagnostic evidence to promote its diagnostic accuracy. Methods The radiological appearances of squamous cell carcinoma arising in chronic ulcers, sinus and scars of the lower limbs in 16 patients from February 1968 to April 2002 confirmed by pathology, were analyzed retrospectively. All cases were taken posterior-anterior and lateral radiograph before operation. There were 15 males and 1 females aging from 15 to 67 years (mean, 50.4 years). The lesion was located at the front of tibia in 15, and the middle inferior part of femur. The medical history of primary diseases varied from 1 to over 50 years with an average of 20 years and 3 months. Of 16 cases, the duration of 8 cases from a sudden change of state of an illness to the final diagnosis was 4 to 6 months, 4 cases above 1 year, and 4 cases were found with no any change of state of an illness. Results There were 14 cases observed as typical chronic osteomyelitis, which demonstrated peripheral osteolytic defect, 13 cases with osteolytic destruction spreading outwards from front of tibia, and 1 case with widespread osteolytic destruction in the middle and inferior part of femur. Floating-ice-like rudimental bone in the defect was found in 11 cases, and 4 case were observed with old fracture. 2 cases without obvious history of typical chronic osteomyelitis, 1 case with soft tissue trauma and 1 case of burn, showed small Saucer-like erosion of the cortex and huge lobar soft tissue masses. Moreover, patchy shadow and Codman's triangle were found in 1 case of burn. Furthermore, lobar soft tissue masses and huge ulcer accord with osteolytic destruction was observed in all cases, and soft tissue masses were larger than the area of bony destruction in 6 cases. Conclusion Roentgenogram could display shape, location and extension of bone invasion from skin of the lower limbs that were associated with malignancy, which contribute to diagnose the lesion and provide useful evidence for surgical plan.

The Polarographic and voltammetric behavior of Cd (Ⅱ)-meso-tetrakis(p-sulfophenyl)prophyrin (TPPS4） complex was defined and the cyclic voltammetric peak was obtained at 1.200 V (vs. SCE) in 0.3 mol/L NaoH solution. The characteristics of the peak have been examined in detail and the experimental results prove that the peak has adsorption properties. In addition to the number of electrons transferred in the reaction and the complex ratio, the stability const ant (β=1.19×107) was calculated. The peak current was proportional to the concentration of Cd (Ⅱ) over the rang 3×10－7～1×10－5 mol/L and the limit of detection was 6×10－8 mol/L. Furthermore a possible mechanism was proposed for the reduction of the complex.

Objective To observe the effect of Ultra-micro-LiuweiDihuang Decoction on the embryo brain development and the influence on brain growth hormone(GH),Somatostatin(SS)in prengnant mice of passive smoking,then to demonstrate the relationship between brain and kidney in TCM.Methods Animals were divided into 5 groups at random:normal group(A),model group(B),traditional Liuwei Dihuang Decoction group(C),1/3 dosage of Ultra-micro-LiuweiDihuang Decoction group(D)and all dosage of Ultra-micro-LiuweiDihuang Decoction group(E).The IUGR model was established by passive smoking.On the 19th day of gestation,all mice were anatomized to scale the body and brain weight of lively embryos,then a part of brain tissue was homogenated.The levels of GH and SS in brain tissues were measured by ELISA.Results Passive smoking could decrease the body and brain weight,influence the level of GH and SS in brain tissues.Compared with A and B groups,the C,D and E groups could increase body and brain weight,regulate the level of GH and SS,improve the brain development of IUGR mice obviously(P

OBJECTIVE To explore the risk factors and protective measures on ICU attendants.METHODS According to the risk factors of ICU occupational exposure,we developed and standardized the implementation of the protective measures.RESULTS The occupational exposure protection awareness,prevention methods,and self-protection were improved.CONCLUSIONS The education about occupational of exposure can improve the management of occupational safety and ensure the protection of physical and mental health of attendants,and to prevent the occurrence of hospital infection.

Objective To observe the clinical characteristics in the patients with post infectious irritable bowel syndrome(PI-IBS) and patients with non-PI-IBS(NPI-IBS).Methods Ninety-three patients with irritable bowel syndrome(IBS) treated in our hospital from March 2014 to June 2015 were selected,including 38 cases of PI-IBS and 55 cases of NPI-IBS.The differences of serum inflammatory cytokines levels,gastrointestinal score and gastrointestinal function were compared between the two groups.Resuits The levels of CRP,IL-18 and IL-6 in the PI-IBS group were significantly higher than those in the NPI-IBS group,and the difference was statistically significant (P＜0.05);the level of GAS,VIP and MOT in the PI-IBS group were significantly lower than those in the NPI-IBS group,the difference was statistically significant(P＜0.05);the bowel sounds score,abdominal distension and pain score and gastrointestinal reaction score in the PI-IBS group were obviously higher than those in the NPI-IBS group,the difference was statistical significance (P＜0.05);the various dimensional scores of quality of life in the PI-IBS group were lower than those in the NPI-IBS group,and the difference was statistically significant (P＜0.05).Conclusion The levels of serum inflammatory cytokines in PI-IBS patients are higher,moreover the gastrointestinal function is poorer than that in NPI-IBS patients,which can be the indicators of clinical differentiation.

Objective To study the clinical efficacy of self-made Shengji ointment on wound healing of postoperative perianal abscess. Methods 80 hospitalized patients with simple low anal abscess after surgery were randomly divided into control group and treatment group according to random digital table method, 40 cases in each group.The self-made Shengji ointment was smeared perianally and covered with gauze in treatment group, and Mayinglong shexiang zhichuang ointments was smeared perianally and covered with gauze in control group.VAS pain score and the granulation tissue scoring criteria were used to evaluate the growth status of the granulation.Results The VAS score in two groups were lower on the 3rd, 7th and 15th day than that on the 1st day (P<0.05);the VAS score on 1st day of two groups had no statisticall significant difference, but the VAS score in the treatment group were significantly lower than that in control group on the 3rd, 7th and 15th day, respectively (P<0.05).Duration of pain in treatment group was significantly lower than that of control group (P<0.05).The granulation tissue score in two groups on the 3rd, 7th and 15th day were lower than that on the 1st day,respectively (P <0.05); the granulation tissue score on the 1st day of two groups had no statistically significant difference, but the granulation tissue score in treatment group on the 3rd, 7th and 15th day were significantly lower than that in control group, respectively(P <0.05). Conclusion Self-made Shengji ointment could significantly relive the pain in wound, and promote the growth of granulation tissue, which has better effect in the treatment of wound healing of postoperative perianal abscess than Mayinglong shexiang zhichuang ointments.

Objective To summarize the clinical symptoms of patients with non-erosive reflux disease(NERD) and reflux esophagitis(RE),which is helpful to the differential diagnosis.Methods Outpatients who met the criteria of NERD or RE according to the Montreal definition in Gastroenterology Department Wuhan Union Hospital from 2010-2014 were enrolled in our study.Clinical data were comprehensively collected.Incidence of disease,severity,frequency of esophageal and extraesophageal symptoms,and the rates of overlapping with functional dyspepsia (FD) or irritable bowel syndrome (IBS) were all studied.Results Totally 446 subjects were recruited,including 225 patients with NERD and 221patients with RE.The occurrence rates of esophageal symptoms including heartburn [76.0％ (171/225) vs 52.0％ (115/221),P ＜ 0.01] and acid regurgitation [74.7％ (168/225) vs 54.3％ (120/221),P ＜0.05] in NERD group were significantly higher than those in RE patients,with more severe and frequent (P ＜ 0.05).Despite the rates of food regurgitation were similar,NERD patients behaved more severely and frequently (P ＜ 0.05).Extraesophageal symptoms including throat burning and foreign body sensation in NERD group [40.9％ (92/225) vs 27.6％ (61/221),42.2％ (95/225) vs 31.7％ (70/221),all P ＜0.05] were also higher than those in RE group,the degree of which was more severe too (P ＜ 0.05).RE patients claimed a higher proportion of chronic cough.The incidences of overlapping with IBS in two groups were similar.But there were more patients with FD in NERD group [72.0％ (162/225) vs 62.9％ (139/221),P ＜ 0.05] than in RE group.Conclusions The menifestations and degree of esophageal and extraesophageal symptoms in patients with NERD or RE are different,as well as comorbidities such as FD and IBS.These results suggest that NERD and RE are independent diseases.