The paper aimed to review and update the studies on umbilical cord blood mesenchymal stem cells (UCMSCs) by retrieving Pubmed database and Chinese Journal Full-text Database. As a pluripotent stem cell, UCMSC exhibits abundant source, simple collection, preservation and transport with no allograft injection or ethical issues. UCMSCs can differentiate into cells of hematopoietic system and into osteoblast, chondrocyte, adipocyte, myocyte, neurocyte and hepatocyte under specific culture conditions. UCMSC surface antigen has no specificity. It has the features of mesenchymal cells, endothelial cells and epithelial cells. UCMSCs are widely used to treat many difficult diseases such as encephalomyelopathy, heart disease, diabetes mellitus, necrosis of the femoral head, and vascular occlusion of the lower limbs.

OBJECTIVE:To discuss the medication safety of pregnant women.METHODS:The package inserts of drugs used for the pregnant women in our hospital was compared with the reference books and China Pharmacopeia-Clinical Medication Notice in respect of the indications and medication safety and so on.RESULTS:The relative contents in are not unified in the above 3 documents.The indications defined in reference books cover a wider range than those in drug package inserts and China Pharmacopeia-Clinical Medication Notice,and the condition about contraindication in pregnancy in China Pharmacopeia-Clinical Medication Notice is looser than in drug package inserts.Drugs which fall out of the scope of indications or contraindicated were used in pregnant women.The drug manufacturing enterprises had confined the medication indications for pregnant women to evade risks.CONCLUSIONS:There are hidden risks for the medication of pregnant women. The concerned government departments ought to unify the medication for pregnant women so as to ensure their medication safety and rational drug use.

Objective　To explore the changes and significance of the circulating immunocomplexes(CIC) in patients with chronic iridocyclitis.Methods　We assessed circulating immunocomplexes in peripheral blood of 45 patients with recurrent uveitis and 42 healthy controls by PEG deposition with the biochemical analyzer to obtain levels of CIC different with recurrent uveitis.Results　Levels of CIC were significantly higher in active patients(27.358±6.729)than those in controls(13.459±6.164,P＜0.001) and inactive patients (16.248±6.233,P＜0.001),but patients (inactive) had significantly higher levels than those in controls (P＜0.05).Conclusion　Levels of CIC were found in the groups of active patients with recurrent uveitis (27.358±6.729), but levels of CIC in inactive patients fell. The result of this study suggest that recurrence of uveitis is related to immunocomplexes,and changes in the levels of CIC could be also a measure standard of therapeutic effects.

OBJECTIVE To study the diagnostic and therapeutic conditions in a general hospital,and try to find out the measures to decrease cross infection in hospital. METHODS From Dec 2003 to Nov 2004,2 037 cases of infectious diseases were diagnosed and treated in our hospital.All these cases were analyzed. RESULTS Among the 2037 cases,the most common diseases were viral hepatitis and pulmonary tuberculosis.And others were infectious diarrhea,gonorrhea,syphilis,typhoid,paratyphoid,epidemic parotitis,bacillary dysentery,epidemic encephalitis B,measles,tetanus of newborn and rabies. CONCLUSIONS To control cross infection in hospital effectively,we must pay more attention to standardized management of infections diseases,strengthen the isolation and protective conciousness of medical personnel for infectious diseases,and tighten up the management of sterilization and isolation.

To investigate the effect of Yiqi Huoxue Capsule (YHC) in treating early ventricular remodeling after myocardial infarction.Ninety cases of acute myocardial infarction (AMI) were randomized to two groups: YHC group (A) and placebo group (B). Groups A and B were treated with YHC and placebo respectively on the basis of thrombolytic treatment and/or routine western treatment. Interventricular septum (IVS) thickness, left ventricular posterior wall (LVPW) thickness, left ventricular dimension in end-diastole (LVDd), scoring of local ventricular wall function and left ventricular ejection fraction (LVEF) were observed 1 and 8 weeks after treatment. Eight weeks after treatment, IVS thickness, LVPW thickness and LVDd were increased and scoring of local ventricular wall function was also increased in group B as compared with those one week after treatment;no obvious changes were found in group A and the difference was significant between the two groups (P

Objective To extract and determine the total flavonoids in Fordia cauliflora. Methods Total flavonoids in Fordia cauliflora were extracted by microwave technology and determined by colorimetry. Results Total flavonoids in Fordia cauliflora was 1.12%, and recovery rate was 99.5%, RSD was 2.36%. Conclusion The method is convenient, accurate and can be used for the extraction of total flavonoids in Fofdia caulifora.

Objective To explore the changes of cytokines in myasthenia gravis children and the effect of glucocorticoid on the serum levels of them.Methods Forty cases of myasthenia gravis children were the observation group which was divided to group one (pre-glucocorticoid therapy)and group two (post-glucocorticoid therapy),and 20 cases of healthy children in the same period as the normal control group.The serum levels of cytokines INF-γ,TGF-β1,IL-10 and IL-18 of the observation group one and two and the normal control group were detected by ELISA and were compared between the observation group one and the normal control group and the observation group one and two.Results The serum levels of cytokine INF-γand IL-18 were higher and IL-10 and TGF-β1 were lower in the observation group than in the normal control group,the differences were statistically significant (t =4.45 ~16.72,P <0.01 ).Significant difference of INF-γ,TGF-β1 and IL-18 was found between the observation group one and two (t =8.12 ~10.68,P <0.01)and the sera level of IL-10 had no significant difference (P >0.05).Conclusions Cytokines INF-γ,TGF-β1,IL-10 and IL-18 are involved and probably play different roles in the pathogene-sis of myasthenia gravis in children;Glucocorticoid could affect the secretion of cytokines IFN-γ,TGF-β1 and IL-18 of myasthenia gravis children,which would ultimately to achieve the aim of interfering and con-trolling the clinical symptom of myasthenia gravis in children.

Objective: To observe the effect of acupuncture plus LONG's manual manipulations on the functional improvement of the patients with lumbar intervertebral disc protrusion. Methods: 60 cases of the patients were randomly divided into a observation group and a control group, 30 cases in each group. The observation group was treated with acupuncture plus LONG's manual manipulations, and the control group was treated with single acupuncture. The functions of the lumber vertebrae were processed in accordance with the assessment system of Japanese Orthopedic Association (JOA), and the therapeutic effects were assessed by Visual Analog Scale (VAS). Results: After the treatments, the differences in JOA and improvement indexes were statistically significant (P＜0.05) between the two groups. VAS was decreased much remarkably in the observation group (P＜0.01) than before the treatments and decreased remarkably in the control group (P＜0.05) than before the treatments. There was a remarkable difference in VAS between the two groups after the treatments (P＜0.05).Conclusion: Acupuncture plus LONG's manual manipulations are better than single acupuncture in improving the functions of the patients with lumbar intervertebral disc protrusion.

Objective Diagnosis of contrast-enhanced ultrasound ( CEUS) for small focal liver lesions under the background of hepatocirrhosis has been a difficult problem in clinical practice .CEUS has significantly improved the accuracy of liver cancer diagno-sis, but little research has been done in minimal lesions .The article was to investigate the diagnostic value of CEUS in liver cirrhosis with small focal liver lesions (≤2 cm) . Methods 70 patients with cirrhosis and 76 small focal hepatic lesions who were admitted in our hospital from March 2015 to August 2015 were examined by conventional ultrasound and CEUS .Comparative analysis was made be-tween the results of tests and pathology to calculate the sensitivity , specificity and accuracy of CEUS and conventional ultrasound . Results The sensitivity , specificity and accuracy in diagnosing small hepatic cancer lesions complicated with cirrhosis by CEUS and conventional ultrasound were 47.6%、79.4%、61.8%and97.6%、97.1%、97.4%,respectively.CEUS was in high consistence with the results of pathology ( Kappa 0.947) and significantly higher than those of conventional ultrasound (Kappa 0.259). Conclusion CEUS has high application value in the differential diagnosis of small focal he-patic lesions complicated with cirrhosis .

Abstract To analyse the mechanism of TECA bio-artificial liver support system(BALSS)to treat acute liver failure (ALF) canines. The blood of partial liver resection-induced ALF canines or RPMI1640 solution were circulated in the inner space of the hollow fibers (blood loop), and porcine hepatocytes were cultured in outer space of the hollow fibers (cell loop). The concentrations of lidocaine, albumine and urine in the RPMI1640 solution were measured. The compositions of proteins in cell loop and blood loop were analysed by polyacrylamide gel electrophoresis (PAGE). The ultrastructure of porcine hepatocytes in BALSS was observed with transmission electronic microscopy. There were not any proteins in RPMI1640 solution before BALSS circulation. After 6 hours of the BALSS circulation, the concentration of lidocaine in RPMI1640 solution decreased significantly,and concentration of albumine and urine nitrogen increased gradually. The ultrastructure of the porcine hepatocyte shown damaged after BALSS 6h. The results suggested that TECA BALSS could replace temporarily the functions of failure liver by the effects of biologic transform and synthesis of porcine hepatocytes.