To explore the feasibility of sevoflurane used in obstetric anesthesia and examine its effects on neonatal Apgar scores.A total of 60 cases of term primipara were assigned into epidural anesthesia assisted sevoflurane inhalation group (experimental group,n =30) and epidural anesthesia group (control group,n =30).In the experimental group after skin incision,the patients could not tolerate the anesthesia mask inhalation of sevoflurane.And the control group received no adjuvant drug.The maternal inhalation of sevoflurane to delivery of fetus after 1 min,5 min,10 min Apgar scores were analyzed.And the inhalation of sevoflurane to fetal childbirth duration were 4-10 min in two groups.The intergroup difference of newborn Apgar scores had no statistical significance (P ＞ 0.05).Epidural anesthesia assisted sevoflurane inhalation for cesarean section had no significant effect on neonatal Apgar scores.

American medical education is one of the most characteristic and successful education models in the western country. There are significant differences between American and Chinese medical education. American successful experiences are precious for China to build the first-class medical universities in the new century

Objective To monitor and analyze the distribution of pathogenic bacteria from CSF and its drug resistance change in Yangzhou area during 2011-2015 ,so as to provide the latest evidence for clinical rational use of antibacterial drugs .Methods The VITEK 2 automatic microbiological instrument was applied to identify bacteria and conduct the drug susceptibility test .The distri‐bution and drug susceptibility situation of isolated pathogenic bacteria were analyzed by using the WHONET 5 .6 software . Results In 2074 CSF bacterial culture from 2011 to 2015 ,74 strains(3 .57% ) of pathogenic bacteria were isolated ,in which the top three were Acinetobacter baumanni(21/74 ,28 .38% ) ,Klebsiella pneumonia (13/74 ,17 .57% )and Staphylococcus epidermis(12/74 , 16 .22% ) .The resistance rate of acinetobacter baumanni toantibacterial drugs was extremely serious ,showing muti‐drug or pan‐drug resistant phenomena .Conclusion Regular monitoring and analyzing the species and change of drug resistance in pathogenic bacteria isolated from CSF have an important significance to guide clinic to rationally use antibacterial drugs .

Objective To observe the clinical effects and the safety of S-adenosy-L-methionine ( SAMe ) associated with ursodeoxycholic acid (UDCA) in treating intrahepatic cholestasis of pregnancy (ICP). Methods Eighty patients with ICP were randomly divided into treatment group ( treated with UDCA orally, 250 mg, TID and simultaneously with intravenous SAMe 1. 0 g, qd) and control group (treated with intravenous SAMe 1. 0 g, qd). Pruritus degree, serum total bilirubin (TB), total bile acid (TBA), glycocholic acid (CG), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were analyzed before and after the treatment, and the pregnancy outcomes, such as the rate of premature, uterine-incision delivery and fetal distress were recorded. Results After treatment, the pruritus degree and the levels of TB, TBA, CG, ALT AST were decreased significantly compared with pretreatment in both groups. TB, TBA, AST and ALT of the treatment group decreased from (27.83±9. 34), (45. 62±18. 30) μmol·L-1, (195. 98±30. 22), (188. 69±29. 11) U·L-1 to (11. 81±4. 91), (11. 88±2. 23) μmol·L-1, (73. 59±21. 53), (67. 94±30. 53) U·L-1, respectively, and TB, TBA, AST, ALT of the control group decreased from (27. 49±7. 87), (49. 12±10. 39) μmol·L-1, (211. 93±34. 9), (210. 40±43. 39) U·L-1 to (16. 08± 6.23), (23.88±6.63) μmol·L-1, (87. 20±32. 52), (81. 77±35. 16) U·L-1, respectively (P<0. 05). Compared with control group, improvements of liver function, the rates of premature delivery, fetal distress and uterine-incision delivery were not significantly different in the treatment group (P>0. 05), but the declines of TB, TBA and CG in treatment group were superior to those of the control group (P<0. 05). Conclusion In terms of improving pruritus, liver function and pregnancy outcome, single SAMe application could obtain similar effects compared with SAMe combined with UDCA, but SAMe combined with UDCA is more effective than the single SAMe application in decreasing the level of TBA and CG.

This study was aimed to quickly and accurately identify different origins and categories of commodity edible bird’s nest (EBN) using DNA barcoding technique, in order to reveal its genetic differences. The total genomic DNA was isolated from the EBN samples. And Cytb gene sequences were amplified and sequenced by PCR. Then, 32 sequences were aligned and analyzed with DNAStar and MEGA 6.0 software. NJ phylogenetic tree was constructed. The nearest distance was calculated. The results showed that the original species of 32 samples of EBN were identified.Aerodramus fuciphagus was the genetic origin of 23 white nest samples. AndAerodramus fuciphagus germaniwas the genetic origin of the other 8 samples. The origin of black nest sample wasAerodramus maximusorAerodramus maximus lowi. It was concluded that the genetic origin of different EBN categories was variant. The identification of EBN’s origin species with Cytb sequence was quick and accurate.

Glucose-6-Phosphatase (G6Pase) was regarded as a marker enzyme of the endoplasmic reticulum in a number of different cells. The purpose of this report is to study the localization of G6Pase activity in the rat left ventricular myocardial cells. G6Pase activity was found in the lumen of the nuclear envelope, the sarcoplasmic reticulum(SR) and the subsarcolemmal cisterns. The SR tubules between the adjacent myofibrils displayed characteristic distribution on their longitudinal profiles, as a curtain-like network, the tubules appeared to be tight network facing A-band, whereas tubules formed large polygonal meshes facing I-band. It is thought that the SR tubules facing A- and I-bands, respectively, represented an adaptation of SR to the selective shortening of the myofibrils at the I-band during contraction.

Left ventricular myocardial hypertrophy was produced in the rats by ligation of the abdominal aorta below the diaphragm for seven weeks.Ultrastructurally, it was observed that the nucleus and nucleolus were enlarged, and the density of the chromatin of the hypertrophied myocardial cells was decreased. Free ribosomes and endoplasmic reticulum were increased. Golgi apparatus was well developed and was increased in number.Cytochemically, G6Pase activity was localized in the lumen of the sarcoplasmic reticulum, nuclear envelope and subsarcolemmal cisterns, and it was also positive in the regenerative rough endoplasmic reticulum. TPPase activity appeared in the Golgi apparatus, and it was especially prominent in the Golgi apparatus of the hypertrophied cells.These findings suggest that the protein synthetic activity was increased in the hypertrophied myocardial cells.

Leptin is a kind of energy-modulation hormone expressed by fat tissue. Its receptor has a wide distributing in human body which affects much physiological systems and metabolize access. With the further researches to leptin, its function is not localized fatness and thinness. Leptin is closely associated with the function of gastric mucosa. It attends the pathophysiological course of gastric cancer and gastric -related disease.

To investigate the potential role of interleukin 12 (IL 12) in human acute graft versus host disease (aGVHD). 26 patients undergoing allogeneic hematopoietic stem cell transplantation (allo HSCT) were included in this study. IL 12 protein and gene expression in peripheral blood mononuclear cells were measured using enzyme linked immunosorbent assay (ELISA)and reverse transcription polymerase chain reaction (RT PCR), respectively. The results showed that all the patients achieved engraftment.Among them, 10 patients developed grade ⅠGVHD, 5 patients deveolped grade Ⅲ or Ⅳ GVHD. IL 12 protein and gene expressions in peripheral blood mononuclear cells were significantly higher in patients with aGVHD compared with those without aGVHD.The levels of IL 12 correlated significantly with the severity of aGVHD .The results suggested that IL 12 might play an important role in the development of human aGVHD.

Objective To explore the relationships between serum levels of adiponectin and essential hypertension in the people with overweight and obesity.Methods The body mass index(BMI),waist circumference(WC),waist-hip ratio(WHR) and blood pressure(BP) were measured among 21578 people from 21 to 60 years old in Shenzhen.According to BMI,the people were divided into the normal-weight group(BMI≤24kg/m~2)(n=14817),the overweight group(2495 cm,respectively(all P0.05).The serum levels of adiponectin were negatively correlated with BMI,WC,WHR and systolic blood pressure in the hypertension obesity cases.Conclusion Adiponectin may be intimately related to obesity and essential hypertension.Keeping the BMI and WC at normal range is one of the effective ways to prevent the risk of essential hypertension.