Objective To evaluate the job burnout of the nurses in sterile laminar flow wards and offer proposals for improvements.Methods Forty nurses in sterile laminar flow wards were surveyed with MBI-GS.Results The scores of emotional exhaustion,cynicism,job inefficiency were higher after working in sterile laminar flow wards for 3 months than those working in general wards for 3 months.Conclusions The nurses in laminar flow wards are prone to have job burnout.Standardized training,humanized management and positive psvchological intervention can improve the job burnout of the nurses in laminar flow wards.

OBJECTIVE To analyze the status quo of pathogenic bacteria distribution and drug resistance of hospital infection in intensive care unit,to provide evidence for the guidance of clinical rational administration and to decrease the hospital infection. METHODS Pathogenic bacteria were isolated from patients who suffered from hospital infection in intensive care unit from 2001 to 2004.K-B slip diffusion method was taken to carry out the sensitive test.Rate of drug resistance of the pathogenic bacteria was analyzed. RESULTS As the bacteria distribution,the highest isolated rate was 60.77%,which was isolated from the sample of respiratory tract.The main pathogenic bacteria were the Gram-negative bacilli(47.69%).And the second pathogenic bacteria were the mycetes(37.69%).The Gram-positive cocci(14.62%) occupied the third.The monitoring of the drug resistance showed that rate of the drug fast was rather higher.The detection rate of the meticillin-resistant staphylococci was 81.82%.The detection rate of the ESBLs from the Escherichia coli and the Klebsiella pneumoniae was 75.00% and 57.14%,respectively. CONCLUSIONS Intensive care unit is one of the highest risk departments for the hospital infection.Generally,the pathogenic bacteria are the multidrug-resistant ones.Enhanced monitoring on pathogenic bacteria distribution and drug resistance analyses of hospital infection in intensive care unit could benefit for the guide of clinical rational administration,the depression of multidrug-resistant bacteria,and the decrease in hospital infection.

Background:Intrahepatic cholestasis is a commonly seen clinical manifestation, and often accompanied with jaundice.Study on clinical characteristics of patients with different degrees of jaundice is helpful for the acknowledge of intrahepatic cholestasis.Aims:To explore the clinical characteristics of intrahepatic cholestasis with jaundice.Methods:General data, biochemistry parameters, etiology and treatment of 703 patients with intrahepatic cholestasis were retrospectively analyzed.Results:Jaundice occurred in 168 patients (23.9%), including 149 mild jaundice, 15 moderate jaundice and 4 severe jaundice.Levels of ALT, AST, ALP, GGT, DBIL, TBIL, ratio of DBIL/TBIL, TBA were significantly increased in jaundice group than in non-jaundice group (P<0.05).Levels of ALT, AST, DBIL, TBIL, TBA were statistically different between groups with different degrees of jaundice (P<0.05), however, no significant differences in ALP, GGT, ratio of DBIL/TBIL were seen (P>0.05).The main etiology of intrahepatic cholestasis were digestive system tumors, cardiovascular diseases, shock, hematologic diseases and primary biliary cholangitis.Ursodeoxycholic acid and S-ademetionine were the main drugs for treatment of intrahepatic cholestasis.Conclusions:For patients with intrahepatic cholestasis, levels of ALT, AST, ALP, GGT are increased with the development of jaundice, and attention on damage of hepatocytes should be paid.The etiology of intrahepatic cholestasis with jaundice involves diseases of different organs and systems, most of them are malignant tumor, cardiovascular diseases, shock and primary biliary cholangitis.

Objective To explore the effects of hyperlipidemia on serum levels of matrix metalloproteinase-9(MMP-9) and C-reactive protein(CRP) in the patients with cerebral infarction(CI).Methods Testing the serum levels of MMP-9,CRP and triglyceride(TG) in 36 CI patients [with high level TG(n=18) and with normal TG(n=18)] and 17 normal controls(NC group).The relationship among the serum levels of MMP-9,CRP and TG were analysed.Results The contents of MMP-9,CRP and TG were significantly higher in CI patients with high level TG group than those in normal level TG group and NC group(all P

Objective To observe the changes of the levels of serum soluble vascular adhesion protein(sVAP)-1 and C-reactive protein(CRP) in patients with cerebral infarction(CI).Methods The levels of serum sVAP-1 and CRP were detected in CI patients at acute stage (ACI group,20 cases) and at convalescence (CCI group,21 cases).The results were compared with normal controls(NC group,21 cases).Results The levels of serum sVAP-1,CRP in ACI group were significantly higher than those in CCI group and NC group(all P

Objective To investigate the relationship between the levels of intedeukin(IL)-6, IL-15 and endometriosis (EM). Method The levels of IL-6, IL-15 in the peritoneal fluid (PF) and serum of 74 patients with EM (EM group) and 46 patients without EM (control group) were measured by double-an-tibody ELISA. Results Higher levels of IL-6 in PF and serum were observed in EM group [(1017.81±361.98) ng/L,(455.47±161.52) ng/L]than those in control group [(284.63±70.50) ng/L,(149.37± 43.09) ng/L], and there was significant difference (P<0.01). The levels of IL-6 in PF and serum in EM group with stage Ⅲ-Ⅳ [(1253.44±189.63) ng/L, (556.50±93.34) ng/L]were significantly higher than those in patients with stage Ⅰ-Ⅱ [(582.81±107.75) ng/L, (268.96±63.48) ng/L](P < 0.01). There was positive relationship between the levels of IL-6 in PF and serum (r=0.950, P=0.01). The levels of IL-15 in PF in EM group [(333.45±63.94) ng/L]were significantly higher than those in control group[(203.85± 70.52) ng/L](P<0.01). No significant difference was found in the levels of serum IL-15 between EM group and control group (P>0.05). No significant difference was observed either in the levels of IL-15 in PF and serum between patients with stsge Ⅲ-Ⅳ and stage of EM Ⅰ-Ⅱ (P>0.05). Conclusions The increase of IL-6, IL-15 in PF may contribute to the development of EM. Serum IL-6 levels are of clinical diagnostic value in patients with EM.

Objective To summarize the computed tomography (CT),magnetic resonance imaging (MRI) and fluorine-18 fluorodeoxy glucose (18 F-FDG) positron emission tomography (PET)/CT features of abdominal non-organi lymphangioma.Methods The retrospective cross-sectional study was conducted.The clinical data of 16 patients with abdominal non-organi lymphangioma who were admitted to the Affiliated Hospital of Ningbo University (11 patients) and the Changhai Hospital Affiliated to the Second Military Medical University (5 patients) between January 2009 and December 2015 were collected.All the patients received CT,MRI and 18F-FDG PET/CT examinations.Observation indicators:(1) imaging examinations;(2) imaging features of CT and MRI;(3) imaging features of PET/CT;(4) treatment and follow-up.Operations were applied to patients based on agreements of patients and families after finishing preoperative examinations,and postoperative pathological examinations were also performed.Follow-up using outpatient examination and telephone interview was performed once every 6-12 months postoperatively up to July 2016.Results (1) Imaging examinations:of 16 patients,12 underwent plan and contrast-enhanced CT scans,5 underwent plan and contrast-enhanced MRI scans,2 underwent plan MRI scans and 1 underwent 18 F-FDG PET/CT scan.One patient underwent simultaneously CT,MRI and 18 F-FDG PET/CT examinations and 2 patients underwent simultaneously CT and MRI examinations.(2) Imaging features of CT and MRI:① Classification of lymphangioma:unilocular cyst,plurilocular cyst and lymphangiomatosis were detected in 6,9 and 1 patients,respectively.② Lymphangioma location:9 of lymphangioma located in the single regions (6 with unilocular cyst and 3 with plurilocular cyst),7of lymphangioma involved multiple regions (6 with unilocular cyst and 1 with lymphangiomatosis).③ Lymphangioma diameter:lesion diameter was 4-25 cm,with an average of 12 cm,and diameter in 9 of lymphangioma was more than 10 cm.④ Lymphangioma shape:3 lymphangiomas were regular-shaped,showing round-like changes,and 13 lymphangiomas were irregular-shaped,showing'moulding'-like changes.⑤ Internal structure of lymphangioma:internal structures in 12 patients undergoing plain CT scans presented as liquid low density,with homogeneous density and-10-19 HU of CT value.MRI scans in 7 patients showed low signal on T1WI and high signal on T2WI,with homogeneous signals.No bleeding was found inside the abdominal non-organi lymphangiomas of 16 patients.The fluid-fluid level was detected in 1 patient with lymphangioma due to bleeding in pathological changes of liver.The septa and wall thickness was less than 2 mm in 15 patients with lymphangiomas,and was more than 3 mm in 1 plurilocular cyst lymphangioma,showing a equal density and isointensities on T1WI and T2WI.There was no nodule and calcification in 16 patients.⑥ Lymphangioma boundary:15 patients had clear boundary of lymphangioma and 1 had an unclear boundary of lymphangioma.⑦ Extrusion of adjacent organs:lymphangiomas in 10 patients were local concave,showing arc-shaped impression and hilar depression,lymphangiomas in 4 patients showed surrounding blood vessels,and fat density or signal inside lymphangioma was found in 2 patients.There were no surrounding intestine and lymph node enlargement in 16 patients.⑧ Lymphangioma reinforcement:of 14 patients with contrast-enhanced scans,septa and wall of lymphangiomas demostrated slight enhancement in 13 patients and moderate enhancement in 1 patient,and all contents showed no enhancement.(3) Imaging features of 18F-FDG PET/CT:inhomogeneous uptake of FDG was manifested in 1 plurilocular cyst lymphangioma,metabolic activity of the lesion was slightly higher than liver,SUVmax=3.71.(4) Treatment and follow-up:14 patients underwent single resection of lymphangioma (6 with unilocular cyst and 8 with plurilocular cyst);1 patient with plurilocular cyst lymphangioma underwent resection of lymphangioma,descending part of duodenum,pancreatic head and ascending colon;1 patient with lymphangioma underwent splenectomy due to smaller lesion.Sixteen patients were confirmed with lymphangioma by postoperative pathological examinations.Of 16 patients,13 were followed up for 6-31 months,with a median time of 15 months.During the follow-up,12 patients had no recurrence,with satisfactory outcomes,and 1 with recurrence of lymphangioma underwent multiple cervical and thoracic resections under right arm and sclerotherapy.Conclusion Abdominal non-organi lymphangioma has some imaging characteristics,it usually manifests as a larger mass with plurilocular cyst,moulding,liquid density/signal,thin septa and wall and clearly boundary.Plurilocular cyst lymphangioma has uptake of 18F-FDG.

OBJECTIVE To analyze multi-drug resistant Acinetobacter baumannii(ABA) outbreak of nosocomial infection in the respiratory intensive care unit(RICU),to explore effective measures for prevention and control.METHODS In RICU on April 3 to 30 2009,patients under mechanical ventilation in the respiratory tract with MDRABA were conductd epidemiological surveys.RESULTS On 27 consecutive days,six cases occurred in mechanical ventilation in patients with lower respiratory tract MDRABA infection,of which 5 patients were cured;6 cases of lower respiratory tract of patients in sputum specimens isolated the same resistance spectrum MDRABA.CONCLUSIONS Medical personnel lack the consciousness of washing their hands.Indoor environment,air disinfection machine lack of supervision in mechanical ventilation patients with lower respiratory tract MDRABA,were the main reasons of infection outbreak.Strict isolation of patients should be strengthened,full implementation of hand-washing compliance,education,conventional screening,early intervention to prevent outbreak of hospital infection.

0.05). Conclusion In our samples, this two polymorphisms in A2M might play similar roles in the susceptibilities to PD and AD.

Objective:To preliminarily explore the synthesis of a quercetin derivative 3′,4′,5,7-four-(O-methoxy carbonyl meth-yl) quercetin and its pharmacological activities. Methods:Quercetin as the reactant and N,N-dimethyl-formamide ( DMF) as the sol-vent, the target product 3′,4′,5,7-four-(O-methoxy carbonyl methyl) quercetin was obtained by the slow addition of methyl chloroace-tate in the presence of anhydrous K2 CO3 to introduce ether bond at 3′,4′,5,7- bit. The structure was characterized by LC-MS, 1 H-NMR and element analysis. The nanoemulsion of the product was prepared using a film dispersion method, and with intraperitoneal in-jection, the effect on pituitrin-induced myocardial ischemia cardiovascular system in rats was observed. Results:3′,4′,5,7-Four-(O-methoxy carbonyl methyl) quercetin was successfully synthesized, and could be metastasized to a demethylation product containing dis-tal free carboxyl with increased polarity proved by metabolic tests in vitro. The results of electrocardiogram and animal experiments showed that the compound had improving effects on pituitrin-induced myocardial ischemia in rats. Conclusion: The nanoemulsion of 3′,4′,5,7-four-(O-methoxy carbonyl methyl) quercetin with intraperitoneal injection shows significant antagonism against pituitrin-in-duced myocardial ischemia in rats.