Objective To evaluate the job burnout of the nurses in sterile laminar flow wards and offer proposals for improvements.Methods Forty nurses in sterile laminar flow wards were surveyed with MBI-GS.Results The scores of emotional exhaustion,cynicism,job inefficiency were higher after working in sterile laminar flow wards for 3 months than those working in general wards for 3 months.Conclusions The nurses in laminar flow wards are prone to have job burnout.Standardized training,humanized management and positive psvchological intervention can improve the job burnout of the nurses in laminar flow wards.

Background:Intrahepatic cholestasis is a commonly seen clinical manifestation, and often accompanied with jaundice.Study on clinical characteristics of patients with different degrees of jaundice is helpful for the acknowledge of intrahepatic cholestasis.Aims:To explore the clinical characteristics of intrahepatic cholestasis with jaundice.Methods:General data, biochemistry parameters, etiology and treatment of 703 patients with intrahepatic cholestasis were retrospectively analyzed.Results:Jaundice occurred in 168 patients (23.9%), including 149 mild jaundice, 15 moderate jaundice and 4 severe jaundice.Levels of ALT, AST, ALP, GGT, DBIL, TBIL, ratio of DBIL/TBIL, TBA were significantly increased in jaundice group than in non-jaundice group (P<0.05).Levels of ALT, AST, DBIL, TBIL, TBA were statistically different between groups with different degrees of jaundice (P<0.05), however, no significant differences in ALP, GGT, ratio of DBIL/TBIL were seen (P>0.05).The main etiology of intrahepatic cholestasis were digestive system tumors, cardiovascular diseases, shock, hematologic diseases and primary biliary cholangitis.Ursodeoxycholic acid and S-ademetionine were the main drugs for treatment of intrahepatic cholestasis.Conclusions:For patients with intrahepatic cholestasis, levels of ALT, AST, ALP, GGT are increased with the development of jaundice, and attention on damage of hepatocytes should be paid.The etiology of intrahepatic cholestasis with jaundice involves diseases of different organs and systems, most of them are malignant tumor, cardiovascular diseases, shock and primary biliary cholangitis.

Objective To observe the changes of the levels of serum soluble vascular adhesion protein(sVAP)-1 and C-reactive protein(CRP) in patients with cerebral infarction(CI).Methods The levels of serum sVAP-1 and CRP were detected in CI patients at acute stage (ACI group,20 cases) and at convalescence (CCI group,21 cases).The results were compared with normal controls(NC group,21 cases).Results The levels of serum sVAP-1,CRP in ACI group were significantly higher than those in CCI group and NC group(all P

OBJECTIVE To analyze the status quo of pathogenic bacteria distribution and drug resistance of hospital infection in intensive care unit,to provide evidence for the guidance of clinical rational administration and to decrease the hospital infection. METHODS Pathogenic bacteria were isolated from patients who suffered from hospital infection in intensive care unit from 2001 to 2004.K-B slip diffusion method was taken to carry out the sensitive test.Rate of drug resistance of the pathogenic bacteria was analyzed. RESULTS As the bacteria distribution,the highest isolated rate was 60.77%,which was isolated from the sample of respiratory tract.The main pathogenic bacteria were the Gram-negative bacilli(47.69%).And the second pathogenic bacteria were the mycetes(37.69%).The Gram-positive cocci(14.62%) occupied the third.The monitoring of the drug resistance showed that rate of the drug fast was rather higher.The detection rate of the meticillin-resistant staphylococci was 81.82%.The detection rate of the ESBLs from the Escherichia coli and the Klebsiella pneumoniae was 75.00% and 57.14%,respectively. CONCLUSIONS Intensive care unit is one of the highest risk departments for the hospital infection.Generally,the pathogenic bacteria are the multidrug-resistant ones.Enhanced monitoring on pathogenic bacteria distribution and drug resistance analyses of hospital infection in intensive care unit could benefit for the guide of clinical rational administration,the depression of multidrug-resistant bacteria,and the decrease in hospital infection.

Objective To explore the effects of hyperlipidemia on serum levels of matrix metalloproteinase-9(MMP-9) and C-reactive protein(CRP) in the patients with cerebral infarction(CI).Methods Testing the serum levels of MMP-9,CRP and triglyceride(TG) in 36 CI patients [with high level TG(n=18) and with normal TG(n=18)] and 17 normal controls(NC group).The relationship among the serum levels of MMP-9,CRP and TG were analysed.Results The contents of MMP-9,CRP and TG were significantly higher in CI patients with high level TG group than those in normal level TG group and NC group(all P

ObjectiveTo investigate the clinical efficacy of focused ultrasound for treating non-neoplastic epithelial disorders of vulvar. Methods212 patients with non-neoplastic epithelial disorders of vulvar were treated with focused ultrasound. The patients were followed up after the treatment,and symptom was observed and the efficacy was evaluated. ResultsThe efficacy of non - neoplastic epithelial disorders of vulvar of squamous hyperplasia,lichen sclerosus and mixed dystrophy was 99.2％, 100. 0％, 88.9％ respectively, the difference was not significat (P ＞ 0.05 ). The efficacy in different courses of patients was 100.0％ ( ＜ 2 years), 100.0％ (2 ～10 years) ,91.3％( ＞ 10 years) respectively,the difference was not significat( P ＞ 0.05). ConclusionFocused ultrasound was obviously effective in the treatment of patients with non-neoplastic epithelial disorders of vulvar.

A turn on model of electrochemiluminescence ( ECL)-Pb2+ biosensor based on G-quadruplex of aptamer probe was developed by using quantum dots ( QDs) as labels. The ECL-Pb2+ biosensor was prepared by immobilizing a hairpin aptamer probe with a thiol group at 5 '-end and an amino group at 3 '-end on gold electrode via an Au-S bond. In the presence of Pb2+, the G-rich hairpin aptamer probe opened the stem-loop and formed G-quadruplex, which exposed the amino group at 3'-end and then covalently linked to the carboxyl group on the surface of CdS quantum dots. The fabrication and reaction process of the Pb2+ biosensor were characterized. The responsive ECL intensity was linearly related to the Pb2+ concentration in the range of 2. 0×10-10-5. 0×10-8 mol/L, with the detection limit of 3. 74×10-11 mol/L. In addition, the ECL biosensor exhibited high sensitivity, good selectivity, satisfying stability, and repeatability.

Objective:To preliminarily explore the synthesis of a quercetin derivative 3′,4′,5,7-four-(O-methoxy carbonyl meth-yl) quercetin and its pharmacological activities. Methods:Quercetin as the reactant and N,N-dimethyl-formamide ( DMF) as the sol-vent, the target product 3′,4′,5,7-four-(O-methoxy carbonyl methyl) quercetin was obtained by the slow addition of methyl chloroace-tate in the presence of anhydrous K2 CO3 to introduce ether bond at 3′,4′,5,7- bit. The structure was characterized by LC-MS, 1 H-NMR and element analysis. The nanoemulsion of the product was prepared using a film dispersion method, and with intraperitoneal in-jection, the effect on pituitrin-induced myocardial ischemia cardiovascular system in rats was observed. Results:3′,4′,5,7-Four-(O-methoxy carbonyl methyl) quercetin was successfully synthesized, and could be metastasized to a demethylation product containing dis-tal free carboxyl with increased polarity proved by metabolic tests in vitro. The results of electrocardiogram and animal experiments showed that the compound had improving effects on pituitrin-induced myocardial ischemia in rats. Conclusion: The nanoemulsion of 3′,4′,5,7-four-(O-methoxy carbonyl methyl) quercetin with intraperitoneal injection shows significant antagonism against pituitrin-in-duced myocardial ischemia in rats.

AIM:To investigate the effects of P34H gene silencing on the expression of P34H and activity of hyaluronidase (HYD) in mouse sperm.METHODS:The recombinant plasmid series of P34H targeted short hairpin RNA (shRNA) were constructed by GV248 plasmids vector.These recombinant plasmids were transformed into DH 5αcompetent cells, and the plasmids were taken from DNA sequencing analysis .The HEK293T cells were co-transfected with shRNA and lentiviral packaging plasmids .The 3 kinds of recombinant lentiviruses and negative control lentiviruses were used to in -ject into the mouse epididymis and the expression of P 34H at mRNA and protein levels was detected by real-time PCR and Western blot, respectively.The location of P34H protein on the mouse spermatozoa was determined by indirect immunofluo-rescent staining using P34H antibody.The positive rate and activity intensity of HYD was detected by modified sodium hya-luronate-gelatin membrane.RESULTS:DNA sequencing analysis confirmed that the 3 P34H-shRNA sequences were suc-cessfully inserted into the lentiviral vectors .P34H expression in epididymis tissue was significantly decreased at both mR-NA and protein levels compared with those of the non-transfected and normal control vectors (P<0.05).The GV-P34H-shRNA-1 played a significant role in reducing the percentage of P 34H positive rate and the activity of HYD in mouse sperm.The silencing effect did not significantly differ between the non-transfected and normal control vectors .CONCLU-SION:Silencing of P34H significantly inhibits the percentage of P 34H positive rate and the activity of hyaluronidase in mouse sperm.

0.05). Conclusion In our samples, this two polymorphisms in A2M might play similar roles in the susceptibilities to PD and AD.