Objective To investigate the expression of glucose transporters(GLUTs)in placentas of pregnant women with abnormal glyeometabolism,and to explore its effect on glucose transport between mother and fetus and its relation with the birth weight.Methods Placentas of 41 pregnant women with abnormal glycometabolism(7 cases of DM,10 GDM A1,10 GIGT and 14 GDMA2)and 15 normal pregnant women as control were selected.The expression of GLUT1 and GLUT3was detected by immunohistochemistry.The birth weight was measured at delivery.Results GLUT1 was expressed in the syncytiotrophoblasts and cytotrophoblasts,whereas GLUT3 in some endothelial cells.The expressions of GLUT1 and GLUT3 were significantly different among the five groups(P＜0.01).Positive correlation was shown between the GLUT1 expression and the birth weight(rs=0.532,P＜0.01),but not in GLUT3 expression.Conclusions The expression of GLUT1 and GLUT3 in placentas of pregnancy with abnormal glycometabolism is enhanced,and GLUT1 may play a predominant role in the fetal glucose uptake.

Objective To investigate the rivalry mechanism of ginkgo in rat brain edema after intracerebral hemorrhage.Methods Totally 80 healthy Wistar rats were divided into 4 groups randomly(20 rats in each group).Fifty microliters saline(group A and group B) or auto-hemoglobin(group C and group D) was infused into the right caudate nuclears using stereo tactic guidance.Groups A and C were lavaged 50 g/L carboxymethyl cellulose sodium solution of 3 mL,groups B and D were lavaged the same amount of ginkgo(200 mg/kg) solution dissolved by 50 g/L carboxymethyl cellulose sodium solution once a day for 3 weeks.All the rats were decapitated after raised for 3 weeks,and superoxide dismutase,malondialdehyde,and the number of hemeoxygenase-1(HO-1) positive cells in brains were assayed.Results The differences in SOD and MDA contents as well as HO-1 positive cell number between every two groups were significant(P

Objective To investigate the value of prevention of cerebral ischemia with multi-modality neuroelectrophysiological monitoring in intracranial aneurysm embolization. Methods The clinical data of 44 patients with intracranial aneurysm treated with endovascular embolization from May 2013 to June 2014 were analyzed retrospectively. The patients were divided into two groups according to whether they used intraoperative neuroelectrophysiological monitoring. There were 21 patients in a monitoring group and 21 in a non-monitoring group. According to the site of the aneurysms,somatosensory evoked potential ( SEP),motion evoked potential( MEP),scalp electroencephalogram,and brainstem auditory evoked potential (BAEP)were monitored,and at one day after procedure,their newly developed neurological deficits and the follow up observation after 3 months in both groups were compared and analyzed. Results The intraoperative SEP,MEP,and scalp EEG changes in the monitoring group were observed in 9,3 and 4 patients,respectively. Timely preventive measures were taken for 9 of the patients with cerebral ischemia revealed by neuroelectro-physiological monitoring. At the first day after procedure,the incidence of patients with new neurological deficit was 17. 4%(4/23)in the monitoring group,and that was 47. 6%(10/21)in the non-monitoring group. There was significant difference between the 2 groups(χ2 =4. 623;P<0. 05). There was significant difference in the good prognosis rates(87. 0%[20/23]vs. 57. 1%[12/21])after 3-month follow-up (χ2 =4. 919;P<0. 05)between the 2 groups. There were no deaths in both groups. Conclusion Multimode in combination with neuroelectrophysiological monitoring may decrease ischemic complications in aneurysm interventional treatment and improve the safety of procedure.

Survivin is the most recent member of the IAP family, which shows a higher expression in almost all tumor cells, but not detected in most normal cells. Our attention is drawn by survivin due to its selective expression, which was taken as one molecule sign of tumor cells. Accordingly, survivin is examined in most leukemia cell line and leukemia specimen, whose expression also showed the association with leukemia. As in other tumor cells, survivin also plays an important role in the leukemogenesis. Meanwhile the detection of survivin can provide an useful prognosis information. This article is a brief overview of the development of survivin and its relationship with leukemia.

An EPA and DHA enriched fish oil ( containing EPA 0.24 g/L, DHA 0.47g/L ) was administered by gastric intubation at the dose of O.2ml/100g wt for a period of one month in the hyperlipoprotein-emia rats caused by high lipid and cholesterol feeding. The administration of fish oil resulted in reduction in TG ( Triglyceride ) ; TC ( Total Cholesterol ) and VLDL-C + LDL-C levels in serum. Levels of HDL2-C and ratio of HDL2/HDL3 were elevated although there was no significant change in level of HDL-C. The study suggests that oral administration of EPA and DHA enriched fish oil might reduce the developoment of atherosclerosis.

Objective To establish a new,rapid,simple and reliable assay for detecting autoantibody SSB.Methods A new dot immunogold filtration assay(DIGFA) was developed,in which the recombinant SSB protein expressed in Pichia pastoris was bound to nitrocellulose(NC) membrane and colloidal gold-labeled staphylococus protein A(SPA) was used as an indicator.Results The sensitivity and specificity of DIGFA were 100% and 98.75%,respectively.The agreement between DIGFA and ENA dot assay was 99.01%.Conclusion DIGFA for detecting autoantibody SSB is a good,rapid,simple and accurate assay for clinical diagnosis.

Objective To investigate the features of smog disease.Methods The clinical manifestations and inducements of smog disease patients were comprehensively analyzed by using clinical statistical methods.Results Smog disease mainly invaded young people.Smog disease was found mostly acute or subacute in onset.21 cases invaded internal carotid arterial system among these 30 patients,including infarction and cerebral hemorrhage;6 patients showed vertebral arterial signs,and 3 cases invaded both the two systems.Brain CT or MRI only showed the position of neurological disorder;The final diagnosis was made by DSA.Conclusion Smog disease could invade both the two systems of intracerebral artery,DSA is the best method to diagnose smog disease.

ObjectiveTo explore the role of high volume hemofiltration (HVHF) in preventing and treating multipleorgandysfunctionsyndrome(MODS)by observingthealterationsof plasma lipopolysaccharide (LPS) and its receptor CD14.Methods Twelve Beagle dogs were subjected to hemorrhagic shock and injection of LPS to set up MODS model, and then dogs with MODS were randomly (random number) divided into two groups: HVHF group (n =6) and MODS group (n =6). HVHF was performed about 24 hours after LPS injection in MODS dogs of HVHF group. LPS, CD14 and organ function variables were determined before operation (T1 ), before LPS injection (T2 ) and 0 h (T3) , 3 h (T4 ), 6 h ( T5 ), 9h (T6 ), 12 h (T7), 15 h ( Ts ) and 24 h (T9) after LPS injected. The levels of LPS were determined by using rapid microbiological kinetic reader (MB-80). CD14 was measured by using ELISA. Differences in LPS, CD14 and organ function at different intervals were evaluated by analysis of variance for repeated measures. ResultsCompared with the MODS group, plasma levels of LPS and CD14 were significantly decreased in the HVHF group at T4-9 ( P ＜ 0. 01 ). CD~ were detected in the ultrafiltrate, the sieving coefficients (SC) for CD14 were 0. 81 ±0. 09. LPS could not be detected in the ultrafiltrate. The beneficial effect of HVHF on the hypotension in MODS model was particularly striking at T4-9 (P ＜ 0. 01 ). Mean arterial oxygen tension in HVHF groups at T4-9 was significantly higher than that in MODS group (P ＜0. 01 ).The levels of creatinine and urea nitrogen were decreased significantly in the HVHF group compared with the MODS group at T4-9 ( P ＜ 0. 01 ) Conclusions HVHF restrained excessive inflammatory responses by cutting down the peak value of plasma LPS and CD14, improving the functions of major organs in MODS dogs.

Objective To preliminarily evaluate the performance of glucose detention reagents with three kinds of different chro-mogens and to investigate their anti-interference performance according to NCCLS document.Methods According to the protocol EP10-A2 provided by the National Committee for Clinical Laboratory Standards(NCCLS),the samples with low,middle and high level of glucose were detected by the glucose reagent kits with 3 kinds of different chromogens.The bias,total imprecision,inter-cepts,slope rates,nonlinearities,carryover rate and drifts were calculated.The interference evaluation test was performed according to the document EP7-A2.Results The bias and total imprecision of three kinds of reagent kits were all within allowed ranges.No statistically significant differences were showed in intercepts,slope rates,nonlinearities,carryover rate and drifts.1450 turbidity chyle,5 g/L hematoglobin and 0.03 g/L vitamin C did not interfere with the assay of three kinds of glucose reagent kits with differ-ent chromogens.342 μmol/L free bilirubin,342 μmol/L conjugated bilirubin did not interfere with the detection of reagent with MAOS.Conclusion The glucose detention reagents with three different chromogens have good accuracy and precision,and various performance indexes all conform to the clinical application requirements,reagent with chromogen MAOS is better than other chro-mogenic reagents in the anti-interference performance.

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