ObjectiveTo investigate the personal or clinical characters of those traumatic brain injury (TBI) patients who accept rehabilitative therapy in the past decade.Methods280 inpatients were observed and evaluated at admission. Database was then established. ResultsThe average age was (33.1±11.4), and the ratio of male to female was 4.28:1. 34.6% of them were office-bearer, and 71.1% of all could gain the socialized medicine. Of the cause, 66.4% were traffic accidents. The time between date of onset and date of admission ranged from 10 days to 6 years, with average 288 days, and only 25.0% patients was admitted less than 3 months after onset. Of them all, 15.7% were diagnosed as posttraumatic seizure and 18.9% as dropsy of brain.ConclusionThe main TBI patients in our hospital were male citizens who had socialized medicine. Too less patients can accept rehabilitative therapy during acute or subacute periods after onset.

Objective To investigate the effect of Toll-like receptor 2(TLR2)on the proliferation of human keratinocytes.Methods Keratinocytes were isolated from the foreskin of children,and subjected to primary culture.Atier 3-5 passages.the kemtinocytes were incubated with various concentrations of peptidoglycan(PGN).a TLR2 agonist.Cell proliferation was detected by MTT colorimetric assay and the suitable concentrations of PGN were determined.The mRNA and protein expressions of Ki67.TLR2.NF-kB p65 and TGF-α were detected by real-time quantitative PCR and Western blot.respectively,in keratinocytes treated witll PGN of 0,1.25,2.5 and 5 μg/mL.Antibody blocking test was utilized to evaluate the effect of blocking TLR2 with specific anti-TLR2 neutralizing monoclonal antibody before incubation with PGN on the expressions of Ki67,TLR2,NF-KB p65 and TGF-α by keratinocytes.Results The proliferation of kemtinocytes was significantly promoted by the incubation with PGN of 1.25,2.5 and 5μg/mL for 24 hours (all P＜0.05),which also increased the expression of Ki67 protein,TLR2 mRNA and protein,and NF-KB p65 protein.Further more,the mRNA expression of Ki67 in keratinocytes was elevated bv PGN of 1.25 and 2.5μg/mL,the mRNA expression of NF-KB p65 elevated by PGN of 1.25μg/mL,and the expressions of TGF-αprotein and mRNA elevated by PGN of 1.25 and 5μg/mL (P＜0.05).The mRNA and protein expressions of Ki67,TLR2,NF-kB p65 and TGF-αwere all inhibited by the blocking of TLR2 before incubation with PGN (a11 P＜0.05).Conclusion Activation of TLR2 bv PGN could induce the over-proliferation of human keratinocytes,likely through promoting NF-rB activation and TGF-α expression.

ObjectiveTo investigate long-term outcome after severe traumatic brain injury (TBI) with or without posttraumatic seizure (PTS). Methods225 inpatients of severe TBI were analyzed retrospectively, with parts of them followed up for 2～5 years after injury.Results31 cases (13.8%) were diagnosed as PTS. All the PTS patients and 66 TBI patients without PTS were followed up. There were lower scores of quality of life index (QLI) in PTS patients (5.23±2.25) than those without PTS (7.05±2.28).ConclusionThere was a worse long-term outcome in TBI patients with PTS.

Sensory Modality Assessment and Rehabilitation Technique (SMART) is one of the tools for assessing patients with disorders of consciousness (DOC) after brain injury, to detect signs and meaningful responses to the conscious presence of DOC patients, and to optimize the patient's potential communication and motor function through the development of a treatment plan, that can be used in the assessment, treatment, and long-term prognosis of patients with DOC. SMART can effectively reduce the misdiagnosis of DOC due to the environment, long observation and evaluation, the incorporation of family and care teams in the evaluation, and the normalization of the evaluation content. Besides an assessment tool, SMART provides treatment options. At present, the SMART treatment stage has been formalized, which can create personalized treatment plan for patients. The intervention after DOC evaluation can be divided into exploration, strengthening and education. SMART is advantaged in evaluation, treatment and prognosis, that can benefit the standardization of diagnosis and intervention of DOC in China.