Objective: To investigate the effect of ischemic preconditioning(IPC) in the posterior limbs of rats on ischemic reperfusion(I/R) injury of donor pancreas graft,and to analyze the possible mechanism.Methods: With Group Control(n=6,normal) suffered with sham operation,36 streptozocin(STZ)-induced diabetic SD rats were randomly assigned to 6 groups: Group I/R(n=6) received pancreas transplantation alone(PTA),Group IPC(n=6) received pancreas transplantation alone(PTA) plus IPC,which included three cyclings of 5 minutes ischemic and 5 minutes reperfusion before reperfusing donor pancreas,Group IPC+L-NAME(n=6),Group IPC+DPCPX,Group IPC+Nal.and Group IPC+AA received the same treatment with Group IPC,but separately injected NOS antagonist L-arginine methyl ester(L-NAME),adenosine A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine(DPCPX),opioid antagonist naloxone and oxygen radical scavenger ascorbic acid before IPC.The blood glucose was monitored before and after reperfusion,the TNF-? in serum,MDA and MPO in pancreatic tissue were monitored after reperfusion,and apoptotic cells were stained by TUNEL technique 2 h after reperfusion. Results: ①The blood glucose,serum TNF-?,and pancreas MDA and MPO of group(I/R,) Group IPC+L-NAME,Group(IPC+)Nal.and Group IPC+AA were higher than those of Group IPC after reperfusion(P

Objective:To observe the efficacy of sciatic nerve block combined with epidural block for treatment of sciatic nerve pain caused by lumbar intervertebral disc herniation.Methods:Patients with lumbar disc herniation(n=59)were divided into two group.Sciatic nerve block combined with epidural block group(group S,n=30)underwent the epidural drugs injection,and sciatic nerve block at the same time according to peripheral nerve stimulatorevoked motor response.The epidural block group(group E,n=29)received the same epidural drugs injection.This two groups were given injection every 7 days and 4 injections for a total treatment.The clinical effect, VAS scores,complication and subjective satisfaction were evaluated.Results:After the treatment,VAS scores of both groups decreased significantly(P

Objective:To investigate the protective effect of the ischemic preconditioning on intestinal mucosal barrier after the rats' pancreas transplantation and analyze the possible mechanism.Methods:Group control included 12 normal rats received sham operation,36 steptozozin-induced diabetic SD rats were randomly assigned to 3 groups: Group I/R consisted of 12 diabetic rats which received pancreas transplantation alone,Group DIPC consisted of 12 diabetic rats which received pancreas transplantation with ischemic reperfusion in the pancreas graft before ablating donors,Group DIPC consisted of 12 diabetic rats which received pancreas transplantation with ischemic reperfusion in the recipient.The intestinal permeability,absorptional function and morphology,the TNF-?,NO,SOD and amylase activity in serum,MDA,SOD and MPO in intsetinal tissue were monitored 5 day after reperfusion.Simultaneitily,the bacteria translocational ratio of mesentery lymph node,liver and spleen were observed.Results:The TNF-? level,the amylase activity in serum(P0.05),the degree of intestinal mucous injury,the apoptosis index(P

Effects of antineoplastic agents on endocrine system are common, but it is difficult to identify since clinical complaints are subtle, and even more difficult to relate a particular chemotherapeutic regimen to the induced endocrine disturb. This review was organized according to the pattern of endocrine system diseases induced by antineoplastic agents, such as disorders of glucose metabolism, free water clearance, mineral metabolism, lipid metabolism and GH secretion and growth, metabolic bone diseases, adrenal dysfunction, thyroid disorders and reproductive dysfunction. Early detection and treatment of endocrine deficiency may have a significant impact on the quality and duration of life in a cancer survivor.

Oral cancer is a common cancer of head and neck. Regional recurrence and metastasis are the main course of the poor prognosis. But the exact molecular mechanism is still unknown. Studies have showed that microRNA plays an important role in the tumorigenesis, invasion and metastasis of oral cancer. And it is expected to become a valuable diagnostic marker and therapeutic target. This paper makes a review about the microRNA related to oral cancer.

Objective:The International Affective Picture System (IAPS) is developed to provide a set of standardized emotional stimuli for experimental investigations of emotion and attention.However,the concerned parameters have not been systematically assessed or testified in Chinese people living in totally different culture from American.Methods:300 subjects were selected to assess the valence,arousal and dominance of all the 704 pictures in IAPS with the order to make the native assessment in China.Results:The results showed significant difference in dimensions of valence and arousal between assessed scores of Chinese subjects and the original data of IAPS,despite a high correlation was observed between them.On pictures containing intense feeling or sexy which contradicted traditional Chinese culture,the valence scores assessed in China were significantly lower than the original data while the arousal scores were significantly higher.Conclusion:It is concluded that native assessment should be seriously considered to select pictures from IAPS while making research for Chinese subjects allowing for the eastern and western cultural differences.

Objective To investigate the effect of a new cloned full length gene, C2 gene, which encodes human eukaryotic translation initiation factor, on tumorigenesis of human gastric cancer cells in vivo and in vitro. Methods A constructed eukaryotic vector carrying the full length of C2 gene was amplified, purified, and transfected into a gastric cell line SGC7901 cell. The expression of C2 protein was examined with fluorescence activated cell sorting (FACS) and Western Blot. The effect of C2 gene on tumorigenesis of human gastric cancer cells was investigated in vitro and in vivo with methods of clone formation in plate and oncogenesis in nude mouse. Results FACS and Western Blot showed that C2 protein was expressed highly and stably in transfected cells. The average clone formation rate of C2 gene transducted cells is 43.8%, the rate which is lower than that of vector transducted cells (76.9%). In vivo, the time of tumorigenesis of C2 gene transducted cells in nude mouse is prolonged from 1 week to 2 weeks, and the volume of tumor node is smaller than that of vector transducted cells, that is, 1.20 and 5.58 cm 3 respectively ( P

Objective To evaluate the effect of spinal cord stimulation (SCS) on the expression of spinal substance P (SP) and calcitonin gene-related peptide (CGRP) in rats with neuropathic pain.Methods Forty-eight male Sprague-Dawley rats, aged 8 weeks, weighing 250-300 g, were randomly divided into 4 groups (n =12 each) using a random number table: sham operation group (S group) , n europathic pain group (group NP), sham electrical stimulation group (N-SCS group) , and SCS group.Neuropathic pain was induced by chronic constriction injury (CCI) in anesthetized rats.The sciatic nerve was exposed, and 4 loose ligatures were placed on the sciatic nerve at 1 mm intervals with 4-0 chromic catgut.Electrodes were placed into the epidural space at 5 days after CCI in N-SCS and SCS groups, and in addition, SCS was performed at 12-14 days after CCI in SCS group.The mechanical paw withdrawal threshold (MWT) was measured at 1 day before CCI, and 1, 4, 7 and 14 days after CCI.After measurement of pain threshold at day 14 after CCI, the animals were sacrificed, and the lumbar segments (L4-6) of the spinal cord were obtained for determination of the expression of SP and CGRP in the spinal cord by immunohistochemistry.Results Compared with group S, the MWT was significantly decreased, and the expression of SP and CGRP protein and mRNA was up-regulated in NP, N-SCS and SCS groups (P＜0.05).Compared with group NP, the MWT was significantly increased, and the expression of SP and CGRP protein and mRNA was down-regulated in group SCS (P＜0.05).Conclusion The mechanism by which SCS mitigates neuropathic pain may be related to down-regulated expression of SP and CGRP in the spinal cord of rats.

Objective To investigate the relationship between the mechanism of mitigation of neuropathic pain by spinal cord stimulation (SCS) and high mobility group protein box-1 (HMGB1)/Toll-like receptor 4 (TLR4)/NF-κB signaling pathway in the spinal cord of rats.Methods Forty-eight male Sprague-Dawley rats,aged 8 weeks,weighing 250-300 g,were randomly divided into 4 groups (n =12 each) using a random number table:sham operation group (S group),chronic constrictive injury (CCI) group,sham SCS group (S-SCS group),and SCS group.Neuropathic pain was induced by CCI in the animals anesthetized with intraperitoneal chloral hydrate.The sciatic nerve was exposed and 4 loose ligatures were placed on the sciatic nerve at 1 mm intervals with 4-0 silk thread.Electrodes were placed into the epidural space at 5 days after CCI in S-SCS and SCS groups,and in addition SCS was performed at 12-14 days after CCI in SCS group.Paw withdrawal threshold to mechanical stimulation (MWT) were measured at 1 day before operation and 1,4,7,14 days after CCI.After measurement of pain threshold at day 14 after CCI,the animals were sacrificed and the lumbar segments (L4-6) of the spinal cord were obtained for determination of mRNA expression of HMGB1,TLR4,and NF-κB p65 (in the nuclear protein,by real-time fluorescent quantitative PCR),protein expression of TLR4 and NF-κBp65 (by Western blot),and protein expression of HMGB1 (by immunohistochemistry).Results Compared with S group,MWT was significantly decreased after CCI,the mRNA and protein expression of HMGB1 and TLR4 was up-regulated,and the expression of NF-κB p65in the nuclear protein and mRNA was up-regulated in CCI,S-SCS and SCS groups.Compared with CCI group,MWT was significantly increased after spinal cord stimulation,the mRNA and protein expression of HMGB1 and TLR4 was down-regulated,and the expression of NF-κB p65 in the nuclear protein and mRNA was down-regulated in SCS group.Conclusion The mechanism by which SCS mitigates neuropathic pain may be related to inhibition of HMGB1/TLR4/NF-κB signaling pathway in the spinal cord of rats.

Objective To investigate the protective effects of epidermal growth factor (EGF) on pancreas of rats with acute pancreatitis(AP). Methods Seventy-two male Sprague-Dawley rats were randomly divided into 3 groups: Control group, AP group and AP-EGF group. Subcutaneously injection of EGF (0.1 ?g/g) were given to animals in the AP-EGF group after the establishment of the model of AP. The other two groups of animals received the same volume of saline. At 6 h, 12 h and 24 h after induction of AP, 8 animals in each group were sacrificed respectively, 4 ml of blood sample was withdrawn from heart,2 ml for the analysis of amylase activity and 2 ml for MDA content in serum. Ascites was sucked with dry gauzes and was weighed thereafter. Changes of pancreas morphology were evaluated at every time point. The same part of pancreas was removed for measurement of MDA content, apoptotic index (AI) and histologic changes. Results Histologic injury of the animals in the AP-EGF group was milder than that in the AP group. Ascites weight in the AP-EGF group decreased significantly compared with that in the AP group at 12 h and 24 h 〔(4.53?1.29) g vs (6.58?1.47) g, (7.64?1.85) g vs (11.96?2.13) g,P