Collagen crosslinking by ultraviolet A-light and riboflavin is an effective means for increasing corneal rigidity and preventing the progression of keratoconus.Visual acuity can be improved slightly.The maximum effect of the treatment locates in the anterior 300-340?m of the cornea.No statistically significant change is found for endothelial cell density.With more long-term research, prophylactic treatment of keratoconus at the early stage might become possible,preventing the development of keratoconus and additional refractive correction can be realized.

Pediatric dry eye can occur associated with congenital,autoimmune,and inflammatory disorders.It is often misdiagnosed and untreated because of children's inability to participate in the assessment of subjective symptoms,also difficulty to be examined and unawareness of dry eye.So,it is necessary to pay more attention to dry eye in children.Etiology and characteristics of dry eye in children should be explored and understood.Multidisciplinary approaches of diagnose and treatment should be required.

Dry eye is the most common complication after laser in situ keratomileusis(LASIK). Attention should focus on screening of dry eye before LASIK, decreasing risk factors of surgical manipulation and topical drugs effect on dry eye. Proper management before, during and after LASIK can minimize dry eye. (Ophthalmol CHN, 2006, 15:154-155 )

It is not uncommon for corneal endotheliitis in clinic.Misdiagnosis of the disease was often happened because of the insufficient knowledge on it and lacking of specific examine methods.It is the most important method for avoiding misdiagnosis of corneal endotheliitis to recognize its clinic features and detailed inquiring case history.

The preocular tear film has recently been described as a bilaminar structure,including lipid layer and aqueous/mucin gel layer.The lipid layer has two phases,a relatively thick outer layer,containing nonpolar lipids such as wax esters,sterol esters,hydrocarbons, and triglycerides;and a thin polar inner layer,predominantly consisting of phospholipids.It s confirmed that there are differences in meibomian fatty acid composition between healthy and evaporative dry eye patients.Thus,therapies targeted at replenishing or stabilizing the lipid layer are key points to the treatment of evaporative dry eye,either as monotherapy or in conjunction with therapies designed to enhance aqueous production.

A total of 102 cases (204 eyes) with mild ametropic amblyopia underwent refractive correction without any vision training.After wearing glasses,visual acuity was examined every 3 months during a follow-up period of 12 months.After wearing glasses 12 months,201 eyes (98.53％) were treated effectively,175 eyes (85.78％) cured basically and 3 eyes (1.47％) became invalid.The changes of visual acuity were satisfactory(P ＜0.01).The difference of visual acuity between every two observation timepoints were satisfactory(P ＜0.01).Treating mild ametropic amblyopia by refractive correction alone is both simple and effective.And it has an excellent compliance and a low cost.

Objective To evaluate prognostic effects and complications of interventional embolization at different time points on patients with ruptured intracranial aneurysms of Hunt -Hess Ⅳ-Ⅴlevels.Methods Through a retrospective analysis from 2008.1 to 2012.12, we collected 162 from 200 patients who underwent interventional embolization due to ruptured intracranial aneurysms according to the criteria.Patients with serious neurological disorders , coma, and end-stage diseases were excluded.Brain CT scan was performed to identify the spontaneous subarachnoid hemorrhage of the patients , followed by CTA or digital subtraction angiography ( DSA) to ensure the accurate diagnosis of intracranial aneurysms.All of the patients at Hunt-HessⅣ-Ⅴlevels were treated with interventional embolization , and were divided into two study groups:early stage therapy group of 79 patients ( embolization at the time <72 hours after the onset) and late stage therapy group of 83 patients ( embolization at the time ≥72 hours after the onset ).Factors including pre-and post-interventional Glasgow coma score ( GCS ) and Glasgow outcome score , ( GOS) as well as barthel index ( BI ) were statistically compared between the study groups.T-test was performed to compare the data between the two study groups.Chi-square test was taken to evaluate the enumeration data.And the correlation of therapeutic time and prognosis of patients was demonstrated by binary regression analysis.Results In the patients with ruptured intracranial aneurysms at Hunt-Hess Ⅳ-Ⅴlevels, early stage intervention (63.5 ±13.5) resulted in significantly better BI after three months , and demonstrated better recovery in comparison to the late stage intervention group (34.8 ±12.7) ( t=6.127, P<0.05).But no statistical significance of post-operative GCS (6.6 ±1.6 in early therapy group;6.1 ± 1.4 in late therapy group ) and discharging GOS ( 3.0 ±0.7 in early therapy group; 2.9 ±0.7 in late therapy group) was found between the two groups (t=1.822, 0.631 respectively; P>0.05).The early therapy group also presented a significant decrease of complications , such as hydrocephalus (χ2 =6.369, P<0.05 ) and venous thrombosis (χ2 =5.158, P <0.05 ), compared to the late therapy group.Furthermore, Statistical analysis revealed that therapeutic time closely correlated with the prognosis of patients with ruptured intracranial aneurysms at Hunt-Hess Ⅳ-Ⅴ levels ( P =0.009, OR =3.897).Conclusion The early stage therapy can improve the prognosis and decrease incidence of complications , which should benefit the efficient management of the patients with interventional embolization of ruptured intracranial aneurysms.

Humans ; Infant, Newborn ; Intestinal Perforation ; complications ; diagnosis ; Mycoses ; complications ; diagnosis

Objective: To study the effect of zinc deficiency(ZD)on the gene expression of vitamin D receptor(VDR)and CaBP in duodenal mucosa of rats. Methods: Thirty weaning male rats were randomly divided into three groups:ZD,paired-fed(PF),zinc adaquate(ZA). After 15 d feeding, took the duodenal mucosa to extract RNA,and measured the levels of VDR mRNA and CaBP mRNA in duodenal mucosa by real time fluorescent quantity PCR. Results: The VDR gene expression of ZD group was downregulated 88.89% as that of PF group,and 50.00% as that of ZA group. CaBP gene expression of ZD group was downregulated 80.16% as that of the PF group,and 88.50% as that of ZA group. Conclusion: Zinc deficiency, by changing the activity of VDR and the mRNA expression of VDR,affects the transcription of the target gene CaBP,and then the absorption of calcium that causes allo-osteogenesis.

Objective To investigate the chemical constituents in the aerial parts of mangrove plant Avicennia marina. Methods The isolated compounds Ⅰ-Ⅹ were obtained by the combination of silica gel, Sephadex LH-20 column chromatographies and by semi-preparative HPLC separation. Structural elucidation was conducted by the modern spectral method. Results Ten compounds were isolated and identified from the aerial parts of A. marina, including acteoside (Ⅰ), isoacteoside (Ⅱ), syringaresinol (Ⅲ), 5, 7-dihydroxy-3′, 4′, 5′-trimethoxyflavone (Ⅳ), indolyl-3-carboxylic acid (Ⅴ), betulinic acid (Ⅵ), betullin (Ⅶ), lupeol (Ⅷ), avicequinone C (Ⅸ), and kaempferol (Ⅹ). Conclusion Compounds Ⅰ-Ⅵ are isolated from A. marina for the first time.