Objective To investigate the clinical value of platelet parameters for the assessment of liver function in chronic hepatitis B (CHB) liver cirrhosis.Methods A total of 167 patients with CHB liver cirrhosis (CHB liver cirrhosis group) were selected,according to Child-Pugh score of liver function classification:A grade 48 cases (A grade group),B grade 65 cases (B grade group),C grade 54 cases (C grade group).Fifty healthy cases were chosen as control group,then compared blood platelet count (PLT),platelet distribution width (PDW),mean platelet volume (MPV) levels differences between every group,and these indicators were analyzed.Results PLT,MPV,PDW was(88.53 ± 21.36) × 109/L,(9.47 ± 2.02) fl,(12.41 ± 1.16) fl in CHB liver cirrhosis group,and (172.64 ± 37.42) × 109/L,(11.47 ± 2.16) fl,(10.49 ±0.83) fl in control group,and PLT,MPV were significantly reduced (P ＜0.01),and PDW increased significantly in CHB liver cirrhosis group compared with control group (P＜ 0.01).PLT in A,B,C grade group [(123.78 ± 26.35) × 109/L,(84.96 ± 31.62) × 109/L,(61.48 ± 27.13) × 109/L] was lower than that in control group,and there was significant difference (P＜ 0.01).There was no significant difference in MPV,PDW between A grade group and control group (P ＞0.05).There was no significant difference in MPV,PDW between B grade,C grade group and control group [(9.68 ±2.23),(8.65 ±2.19) fl vs.(11.47 ±2.16) fl and (12.26 ± 1.35),(14.13 ± 1.54) fl vs.(10.49 ±0.83) fl](P＜0.05 or ＜0.01).Correlation analysis showed that PLT,MPV was positively correlated with liver function (r =0.365,0.473,P ＜0.05).But PDW was nagtively correlated with liver function (r =-0.512,P ＜0.05).Conclusions Platelet parameters such as PLT,MPV,PDW are good to evaluate the liver function and it's compensatory of CHB liver cirrhosis,and that dynamic changes of the parameters has important clinical significance in the disease assessment and treatment of liver cirrhosis.

Ischemia-reperfusion of the heart leads to the opening of mitochondrial permeability transition pore(MPTP).Transient opening and subsequent closure of the MPTP may lead to the release of cytochrome c and other proapoptotic molecules.If unrestrained,this will lead to the loss of ionic homeostasis and initiate the cascade of necrotic cell death.Direct or indirect inhibition of MPTP provides protection against ischemia-reperfusion injury.Recent researches have shown that transient low-conductance pore opening also provides protection against reperfusion injury.This review gives summarization of the components,molecular mechanism of MPTP,permeability transition in myocardial ischemia-reperfusion and the relationship between MPTP and ischemic preconditioning.This may lead to better protective strategies against myocardial ischemia-reperfusion injury in the future.

Hypoxia could not only induce tumor chemoradiotherapeutic resistance, but also cause strong invasiveness which make tumor inclined to recurrence and metastasis. With regard to the detection of oxygenation condition in tumor tissue, there are many methods, but each has its advantages and disadvantages. Accurate monitoring of tumor oxygenation state plays an important role in therapeutic schedule formulation and implementation which would improve curative effect of tumor.
Animals ; Cell Hypoxia ; Humans ; Hypoxia ; Neoplasms ; blood ; Oximetry ; methods

Nursing simulation teaching is one of the important measures in nursing teaching reform. As an advanced medical teaching model, SimMan synthetic simulation has been recognized by medical educators both at home and abroad for its advantages of high simulation, repeatability, case diversity and process controllability. Our country has relied on the SimMan synthesis simulation person to carry on the nursing simulation teaching for many years, usually as a teaching strategy into the course of teaching. Its advantages and innovation are affirmed by nursing educators and learners, but still face challenges in the design of simulation teaching, the development of functions and the improvement of equipment utilization. It is worthy discussing deeply by nursing educators.

Objective To investigate the effects of panax notoginseng saponins(PNS) on the expression of IL-10 and TNF-α in the brain after focal cerebral ischemia-reperfusion injury in rats,and the mechanism was explored.Methods 30 male adult Sprague-Dawley rats were randomly divided into sham-operation group,ischemia-reperfusion group and PNS treatment group,10 rats in each group.The middle cerebral artery occlusion(MCAO) was established with ischemia-reperfusion group.The rats received continuous gastric lavage for 4 weeks,who were observed for 3 days and then killed to the detect the expression of IL-10 and TNF-αin ischemic region by ELISA,immunohistochemistry and RT-PCR.The behavioral ability score in rats were tested.Results Compared with the sham-operation group and ischemia-reperfusion group,the expression of IL-10 was increased,while the expression of TNF-α was decreased significantly in PNS treatment group,behavior score of PNS treatment group increased significantly than that of ischemia-reperfusion group.Conclusion The panax notoginseng saponins can reduce the neurons injury after focal cerebral ischemia-reperfusion injury in rats partly by regulating the expression of IL-10 and TNF-α in the brain.

The hardcore of clinical medicine program education is to insist on improving the students'practical capability.The efficient methodology is the key to it.In this paper,the authors investigate the practice of Suzhou University and try to find out the ways to assess the graduates in this program.Also,the authors point out the deficiency in the assessment and put forward mending measures in order to improve the quality of clinical medicine graduate program education.

The authors discussed the development of the assistive devices and service in China and overseas by analyzing the calssification, function, demand and difference between China and other countries.

Objective To evaluate the significace of endoscopic diagnosis of gastrointestinal graft-versus-host disease ( GI GVHD) resulted from allogeneic bone marrow transplantation. Methods Five patients with suspected GI GVHD received endoscopic examination and biopsies were taken from antrum, sig-moid colon or the focal lesions. Results The symptoms of GI GVHD included anorexia, nausea, vomiting, watery diarrhea, abdominal pain, GI bleeding etc. The endoscopic appearance in stomach varied from subtle mucosal edema, hyperemia, and erythema necrotic erosion. Besides the above stated, the endoscopic appearance in colon also presented with diffuse mucosal erosion, hemorrhege and ulcer. Histological findings included characteristically crypt epithelial cell apoptosis and sloughing, and lymphocyte infiltration in epithelium and lamina propria. The involvement may be varied from diffuse to focal in stomach or colon. Conclusion Endoscopic and histological evaluation of the stomach and colon and biopsy specimen can be used as the evidences in diagnosing GI GVHD.

Objective To establish a method for determination of psoralen and isopsoralen in Bushen Jiannao Soft Capsule. Methods The HPLC separation was performed on Diamonsil-C18 (250 mm?4.6 mm, 5 ?m) column with a mixture methanol-water (48∶52) as the mobile phase, at a flow rate of 1.0 mL/min. Detection wavelength was at 246 nm, and column temperature at 40 ℃. Results The linear response of psoralen and isopsoralen ranged from 0.010 08～0.252 ?g (r=0.999 8) and 0.009 14～0.228 5 ?g (r =0.999 9). The average recovery was 98.91% (RSD=1.74%) and 99.93% (RSD=1.27%) respectively. Conclusion The method is simple, accurate, and suitable for the determination of the psoralen and isopsoralen in the preparations.