Objective To investigate the relationship of D-dimer(DD) and fibrinogen levels in blood plasma and diabetic nephropathy(DN).Methods According to the results of urinary albumin excretion rate (UAER), 98 cases of type 2 diabetas were divided into simple diabetic mellitus ( SDM), early diabetic nephropathy ( EDN) and clinical diabetic nephropathy (CDN); other 45 healthy persons were employed as control. Plasma levels of DD and the fibrinogen of all groups were examined.Results There was significant difference of DD and fibrinogen levels in all groups as compared with control group (P

Objective To investigate the diagnostic value of biexponential model of DWI in nontypical hippocampal sclerosis (HS) of the youth.Methods DWI with multiple b values were performed on 75 youths with right temporal lobe epilepsy and 20 healthy youths(control group).There were no abnormalities in hippocampus on conventional MRI.11 of 75 patients were proved right HS by surgery and pathology (HS group).Compare the volume of the right hippocampus and their D,D? and f values in HS group and control group.Results There were statistical differences in D and f values between HS group and control group (Z=-3.08,Z=-2.23,P<0.05). There were no statistical differences in the volume of hippocampus and D? values between two groups (Z=1.77,Z=-0.19,P>0.05). Conclusion When HS does not have typical appearances on conventional MRI,D and f values can provide help from diffusion and the perfusion of microcirculation aspects.

Lower urinary tract symptoms due to benign prostatic hyperplasia is of great prevalence among ageing males,and it can markedly affect patients' quality of life.Personalized drug therapy according to genetic characteristics is the ideal choice for this problem.Here we summarized the latest research findings on the associations of gene polymorphisms with drug efficacy and adverse reaction in the treatment of benign prostatic hyperplasia/urinary tract symptoms.

Child ; Combined Modality Therapy ; Drugs, Chinese Herbal ; administration & dosage ; Eye Diseases ; drug therapy ; therapy ; Female ; Humans ; Middle Aged

The membrane fluidity of mitochondria isolated from langendorff perfused rat hearts was studied with ESR spin labeling technique and TBA method was used to detect the content of lipid peroxide. After total ischemia for 40min followed by a 20min reperfusion the 10-DSA region of the mitochondrial membrane showed significantly increased S (0.1(?)2?0.006) and ?_c(7.13?0.09?10~(10)sec) values (i. e. a decrease of membrane fluidity)in comparison with aerobic control (S=0.103?0.007); ?_c=6.86?0.20?10~(-10) sec) (P0.05). In addition, we found that DS182 could remove O_2~? produced by O_2~? generating system. Our results have showed that DS-182 is a potent scavenger of superoxide. The protective effect of DS-182 on mitochondria during ischemia/reperfusion might be due to its antioxidative activity。

This study was performed on rabbits to examine the changes of calmodulin (CAM) and cAMP in the myocardium during ischemia and reperfusion by radioimmunoassay method. The result showed the levels of CaM and cAMP increased at 20 min of ischemia. Although these two parmeters were unchanged at 40 min of ischemia as compared with the changes at 20 min, they were changed during reperfusion at different times of ischemia. At 20 min ischemia following 10 min reperfusion, the content of CaM decreased, the content of cAMP increased. On the basis of 40 min ischemia, reperfusion induced opposite changes: CaM increased, cAMP decreased. The possible mechanism of these changes was discussed.

We studied the protective effects of redix salviae miltiorrhizae (RSM) on the ischemic and post-ischemic reperfusion injury which was made by ligating and reopening of the left ventricular branch of the coronary artery of the open-chest anaethetised rabbits under the supervision of ECG. There were 14 rabbits in each group, 7 of them for the measurement of myocardial lipid peroxide level (MLPL) by the thiobarbituric acid method (Hiroshi Ohkawa, 1979), and the other 7 for measuring distribution of regional myocardial blood flow (RMBF) by the biological radionuclidelabeled particle method (Wang Jingxian, 1985). With increasing duration of ischemia, MLPL increased significantly in the ischemic and post-ischemic reperfusion injured heart. In the case of 60 mins ischemia followed by 30 mins reperfusion, MLPL was much higher than that without reperfusion. However, which compared with that of 90 mins ischemia, no significant difference was found. The recovery of RMBF was only 53.2% after 30 mins of reperfusion following 60 mins of ischemia. The occurance of post-ischemic reperfusion injury was dependent on the duration and severity of ischemia. The injury of reperfusion after long period (60 mins) of ischemia is similar to that of increasing duration of ischemia. No-reflow phenomenon was observed in ischemic zones during reperfusion after 60 mins of ischemia. In the RSM (2g/kg body wt) protected reperfusion group. MLPL was reduced by 56.0% and the recovery of RMBF was increased by 32.0%.

The time-dependent changes in myocardio membrane system during ischemia/reperfusion injury were studied with ligature of the left ventricular branch of coronary artery in rabbit. The permeability of cardiac membrane system being tested by using lanthanum tracer technique and stereological methods. Lanthanum granules were observed in the sarplacosma and sarccpiasmic tubes especially at ischemia for 20min. The pecmeability of membrane system for La~(+++) was more marked when hearts were reperfused for 20min. With the increase in time of ischemia, the changes became more and more serious. A lot of lanthanum granules were entered into mitochondria when the hearts were reperfused after 40min ischemia. There were neither intact mitichondria nor lanthanum granules in mitochondria observed when the hearts were reperfused after 60min ischemia. The structural changes were irreversible at this stage of reperfusion The results of our study indicated that the changes of permeability of cardiac cell for La~(+++) in ischcmia/reperfusion are time-dependent, 40min ischemia is the demarcation line between the reversible and irreversible injury during reperfusion. On the basis of our experiment, we like to propose a new conception of reversible and irreversible injury of reperfusion.

Calcium overload has been discovered in the ischemia and reperfusion injury of the heart recently, but there were less reports about changes in myocardial Ca distribution under this same condition by electronmicroscopy. Using the potassium-pyroantimonate method and X-ray microanalysis, we studied cytochemical calcium localization in the rabbit's myocardium at various time periods of ischmia with or without subsequent reperfusion. At the same time, we had the morphometric estimation of Ca granule volume density in the mitochondria by TAS(texture analysis system) and evaluated Danshen's protection for ischemia and-reperfusion injury. The results showed that mitochondrial Ca granule volume density increased significantly with the development of ischmia and post-ischemic reperfusion, especially during reperfusion following 40min of ischemia, however following 60min of ischemia, Ca granule volume density decreased than that observed following 40 min of ischemia. This meant that the limit time point for reversible and irreversible injury could be in the range of 40～60min of ischemia. Danshen pretreatment to the rabbits reduced Ca granule volume density significantly. This demostruted that Danshen acted as a calcium antagonist and contributed an outstanding protection to the myocardial calcium overload.

Ⅳ, change of phosphocreatine (PCr) content was very similar to thatof ATP. Content of ADP, AMP, Cr and HYPO was negatively correlated to ATP content. NAD content was also reduced. In summary, recently proposed hyperkalemic crystalloiddilluted blood protecting myocardium would not be sufficient enough and further improve-ment is quiet neccessary.