Objective:To compare the marginal microleakage of class V cavity restored with composite resin by 3 different methods. Methods:Class Ⅴ cavity was prepared in 36 human third molars,the teeth were randomly divided into 3 groups(n =1 2)and were repaired with common Z350 resin,pre-heated Z350 resin and Z350 resin inlay,respectively.After thermal cycling for 500 times the teeth were immersed in fuchsin solution for 24 h.Then the samples were sectioned longitudinally and examined for leakage under stereomicroscope.Results:The microleakage of cavity restoration by pre-heated Z350 resin and Z350 resin inlay was significantly low-er than that of the direct restoration with Z350 resin(P≤0.05).No significant difference was found between the groups of pre-heated Z350 resin and Z350 resin inlay.Conclusion:Marginal microleakage of Z350 resin restoration can be decreased by preheating of the resin.

Objective To observe the ultrastructural changes of Candida albicans by the effect of oyster antibiosis protein which was extracted from muscle of oyster.Methods The colonies of candida albicans exposed to oyster antibiosis protein for 24 hours,with a blank control group and the differences of their ultrastructure were studied under electron microscope.Results After affected by oyster antibiosis protein for 4h,the cell walls were lacked and had reductus with pyknotic cytoplasm.16 hours later,most cells became global shape and their cytomatrix components were lost and some vacuoles appeared in the cytoplasm.24 hours later,the cell walls depleted and the cells spitted.Conclusion oyster antibiosis protein affected commendably the function of candida albicans.

Objective To explore the roles of bacterial infection in the pathogenesis and progression of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). Methods The clinical data of 604 patients with ALI or ARDS hospitalized from April 1991 to March 2001 were analyzed. Results (1) The cause of direct lung injury was predominantly ascribed to lung infection, whereas indirect lung injury was due to sepsis. (2) The gram positive cocci (50.76%) and gram negative bacilli (40.15%) in the isolated pathogenic bacteria from patients were approximately similar. Furthermore, Staphylococcus aureus and Pseudomonas aeruginosa were the first and second pathogenic bacteria, respectively. (3) The incidences of ALI and ARDS in infected patients significantly increased with the grade elevation of systemic inflammatory response syndrome (SIRS) ( P

Objective: To investigate the effect of enteral nutrition on proliferation of colon mucusa in long fasting patients. Methods: Ten patients fasted over two weeks were selected.All patients had no any histories of chemotherapy, radiotherapy and immunosuppressive therapy.They received enteral nutrition for tow weeks. Before and two weeks after enteral nutrition,specimens were obtained between sigmoid and descending colon by colonofibroscope.All specimens of colon mucosa were determined by FCM for proliferation index (PI),S phase factor (SPF)and aptosis(Apto). Results: PI,SPF and Apto were ( 4.010 ?1.290)%,(1.904?2.679)% and (1.913?2.021)% in patients fasted over two weeks respectively, and (7.337?2.679)%,(5.367?3.211)% and (1.208?1.488)% after enteral nutrition for two weeks. Conclusions: Enteral nutrition could improve the proliferation of colon mucosa in long fasting patients.

OBJECTIVE:To prepare weishu emulsion and to establish its quality control method.METHODS:Weishu emulsion was prepared with sodium carboxymethylcellulose,etc.as the adjuvants and the content of dyclonine hydrochoride was determined by UV-spectrophotometry.RESULTS:The linear range of dyclonine hydrochloride was2.0～12.0?g/ml(r=0.9999)and the average recovery rate was99.38%,RSD=0.47%,(n=6).CONCLUSION:The emulsion is feasible in preparation method and controllable in quality.

BACKGROUND:Several studies have demonstrated that stem cells can differentiate into vascular endothelial cells, and then further differentiate and form blood capillary. Based on this principle, autologous bone marrow mesenchyrnal stem cell (BMSC) transplantation promotes angiogenesis to treat ischemia in lower limb.OBJECTIVE: To evaluate the angiogenesis in rabbit ischemic limbs following autologous BMSC transplantation using high-frequency two-dimensional ultrasound detection in conjunction with Doppler color-flow imaging examination. DESIGN, TIME AND SEI-FING: A randomized, controlled, animal experiment was performed in the Third People's Hospital of Wuxi between March 2007 and April 2008.MATERIALS: Twenty-four New Zealand rabbits were randomized to a control group and a cell transplantation group, with 12 rabbits in each.METHODS: Ischemia in lower limbs was induced in all rabbits. One week following ischemia induction, the cell transplantation group was injected with 0.5 mL cell suspension, comprising 2×106 BrdU-labeled autologous BMSCs cultured in vitro, through multiple sites in the region of gastrocnemius muscle. Simultaneously, the control group received the same amount of physical saline in the same region.MAIN OUTCOME MEASURES: The initial segment of rabbit femoral artery and superficial femoral artery was subjected to high-frequency two-dimensional ultrasound and Doppler color-flow imaging examinations to measure femoral artery vascular internal diameter, blood flow peak velocity, and blood flow acceleration time. Ischemic muscular tissue was taken for immunohistochemical staining to detect transplanted cell distribution and for pathological examination of angiogenesis. RESULTS: Two weeks following autologous BMSC transplantation, high-frequency ultrasound results revealed that femoral artery internal diameter and blood flow peak velocity were greater, but blood flow acceleration time was shorter, in the cell transplantation group than in the control group (P<0.01). Immunohistochemical staining results demonstrated the presence of BrdU-positive cells. Pathological sections displayed that vascular density was significantly higher in the cell transplantation group than ih the control group. CONCLUSION: Autologous BMSC transplantation is a promising, simple, and effective method of treating ischemia in lower limbs owing to its promotion of angiogenesis. Meanwhile, high-frequency ultrasound detection of femoral artery is an effective, practical method to evaluate the clinical outcomes of autologous BMSC transplantation.

Objective:Studies suggested that some growth factors and their receptors play important roles in the treatment and prognosis of lung cancer.In this study we investigated the expression of epidermal growth factor receptor(EGFR),vascular endothelial growth factor(VEGF),and Ki-67 simultaneously in the tissues of aged patients with non-small cell lung cancer(NSCLC). Methods:The expression levels of EGFR,VEGF,and Ki-67 in 64 cases of aged patients with NSCLC were detected by immunohistochemistry staining.Results:The positive expression of EGFR,VEGF and Ki-67 in the tissue of lung cancer was 59.4%,57.8% and 92.2%,respectively.It is different significantly while compared with control group of benign tissues.The expression levels of EGFR and VEGF are correlated significantly with the pathologic type,differential degree,TNM stage and lymphatic metastasis.The expression level of Ki-67 is related to differential degree and TNM stage of lung cancer.Conclusion:The method of immunohistochemistry to detect multiple biological markers simultaneously is simple and feasible.It can supply important information for the staging and prognoses of patients with NSCLC,and more than that,it can also provide the theoretic basis for the individualized molecular targeting therapy of NSCLC.

Objective: To investigate the value of double source CT(DSCT)in the diagnosis of myocardial bridge(MB)and analyze the correlation of MB-associated coronary atherosclerotic plaque with age.Methods: A total of 417 patients suspected of coronary artery disease(CAD)were divided into a young group(n=137,30-44 yrs),a middle-aged group(n=197,45-59 yrs) and an old group(n=83,60-84 yrs),subjected to DSCT coronary angiography,and analyzed for the differences in the incidence of MB and coronary atherosclerosis.Results: Altogether 76 cases(18.2%) were diagnosed as MB,10(13.2%) in the young,36(47.4%) in the middle-aged and 30(39.5%) in the old group.Of the MB patients,41(53.9%) were complicated with coronary atherosclerotic plaque,2(20.0%) in the young,17(47.2%) in the middle-aged 22(73.3%) in the old group.There were significant statistical differences in the incidence and the nature of coronary atherosclerotic plaque among the 3 groups(P

Objective: To evaluate the effect and safety of continuous veno-venous hemofiltration(CVVH) in the treatment of multiple organ failure(MOF) in senile patients.Methods: Sixteen patients with multiple organ failure,aged over 80 years,were divided into a survival group,who lived more than 20 days,and a non-survival group,less than 20 days after CVVH,and observed for such clinical indexes as of renal function,K+and blood gas analysis,APACHEⅡ scores and complications.Results: After CVVH,eleven of the patients survived for over 20 days,with 1 case up to 3 years.CVVH effected a significant improvement in BUN,Scr,K+and blood gas as well as a marked reduction in complications.APACHE Ⅱ scores decreased significantly after CVVH in the survival group though not in the non-survival group,as compared with those before CVVH,which were significantly lower in the former than in the latter.Conclusion: CVVH is a safe,effective and well-tolerated method for the treatment of MOF in senile patients.Patients with higher APACHEⅡscores have a poor prognosis.

Objective To investigate the effect of flurbiprofen administration time on inflammatory factors in patients with acute suppurative appendicitis after laparoscopic resection. Methods 60 patients with acute suppurative appendicitis resection treated in our hospital from January 2015 to March 2017 were divided into two groups according to the different administration time. The control group received flurbiprofen at the time of operation, and the observation group received flurbiprofen before operation induction; The changes of the indexes of operation and inflammatory factors before and after the operation were compared between the two groups. Results There were no significant differences between the two groups in each operation index; The level of inflammatory factors in the observation group was better than that in the control group 24 hours after the operation, the difference was statistically significant (P<0.05). Conclusion The effect of flurbiprofen is significant before the induction of anesthesia, and the control effect of inflammatory factors is good after the operation, and the time will not affect the postoperative analgesia.