OBJECTIVE:To discuss the prospect of hospital preparation and to find out effective measures for its devel-opment.METHODS:The history of hospital preparation was reviewed,and the way for its survival was discussed on the basis of current situation and newly formed laws and regulations on preparations.RESULTS&CONCLUSION:Hospital preparation can survive and develop only by“controlling scale,guarantee quality,creating specialty and exploiting new drugs”.

The polymorphisms of VNTR loci D1S80 (pMCT 118), D17S30 (pYNZ-22 ) and ApoB3' in blood and tissues were detected by amplified fragment length polymorphism (Amp-FLP) technique,and was applied in the paternity testing cases. The discriminating power (DP) of D1S80,D17S30 and ApoB3'were 0. 962, 0. 956 and 0. 960 respectively. The cumulative probability of paternity exclusion (EPP) of the three VNTR loci was 94. 51 %,higher than that of conventional blood typings.

Hypoxia could not only induce tumor chemoradiotherapeutic resistance, but also cause strong invasiveness which make tumor inclined to recurrence and metastasis. With regard to the detection of oxygenation condition in tumor tissue, there are many methods, but each has its advantages and disadvantages. Accurate monitoring of tumor oxygenation state plays an important role in therapeutic schedule formulation and implementation which would improve curative effect of tumor.
Animals ; Cell Hypoxia ; Humans ; Hypoxia ; Neoplasms ; blood ; Oximetry ; methods

Dihydroartemisinin (DHA), a semi-synthetic derivative of artemisinin, has recently shown antitumor activity in various cancer cells including cancers of cervix, pancreas, prostate, liver and neuroblastoma. Numerous studies in vivo and in vitro indicate that DHA possesses unique antitumor features and appears to be a promising chemotherapeutic agents. Here we systematically review the advances in research of anticancer of dihydroartemisinin, as well as summarize the mechanisms of its inducing apoptosis,delay cell-cycle, inhibitory cell proliferation and downregulate angiogenesis.
Animals ; Antineoplastic Agents ; pharmacology ; Artemisinins ; pharmacology ; Humans

Overexpression of the epidermal growth factor receptor (EGFR) is a common characteristic of head and neck squamous cell carcinomas (HNSCC) , and initiates important signal transduction pathways in carcinogenesis. Now the EGFR is a validated target for cancer therapies in HNSCC. However, the effect of EGFR-targeted therapies is only modest because of primary and/or acquired resistance. Therefore, an improved understanding of the molecular mechanisms of resistance to EGFR inhibitors may establish new treatment options to overcome resistance. In this review, the molecular mechanisms of resistance and the strategies to overcome it were summarized.
Antineoplastic Agents ; Carcinoma, Squamous Cell ; drug therapy ; Drug Resistance, Neoplasm ; ErbB Receptors ; Head and Neck Neoplasms ; drug therapy ; Humans ; Signal Transduction ; Squamous Cell Carcinoma of Head and Neck

OBJECTIVE To evaluate the effect of AG490 on the invasion of Hep2 laryngeal carcinoma cells, and to investigate the role of STAT3 signaling pathway in human laryngeal carcinoma invasion.METHODS Janus kinase inhibitor AG490 was used to inhibit the activation of STAT3 signaling pathway of Hep2 cells.Hep2 cell invasion was detected by quantitative Matrigel invasion assay.The expression of p-STAT3 was measured by flow cytometry. RESULTS The activation of STAT3 signaling pathway and the invasion of laryngeal carcinomas Hep2 cell line were suppressed by AG490.The expression of p-STAT3 and the invasion cell number of Hep2 cell line in the group treated by AG490 for 24h decreased compared with that in control group(P

Nonalcoholic fatty liver disease (NAFLD) is associated with obesity, type 2 diabetes,dyslipidemia, hypertension, and metabolic syndrome.It likely represents hepatic manifestation of metabolic syndrome. The prevalence of NAFLD has been estimated to be 20％-30％ in the general population. However, it is much higher in type 2 diabetic patients. Several epidemiological studies indicate that NAFLD is linked to an increased risk of cardiovascular disease( CVD), which is independent of underlying cardiometabolic risk factors. This suggests that NAFLD may also be actively involved in the pathogenesis. The possible molecular mediators linking NAFLD and atherosclerosis(AS) include the release of pro-atherogenic factors by oxidative stress and inflammation as well as atherogenic dyslipidemia, abnormal adipose factors, and insulin resistance, finally aggravating progression of CVD. In this article, the relationship and possible mechanisms between NAFLD and AS are reviewed.

Objective To assess the clinical diagnosis value of CT guided transthoracic core needle biopsy in patients with pathological changes.Methods The pathological diagnosis was performed after getting the tissue by the groovy needle punctured the lesion.Results The 165 patients included 153 focal pulmonary diseases and 12 diffuse pulmonary diseases,the defined pathological diagnostic rate was 92.1%,false negative rate was 3.9%,18.9%in malignant and benign pathological changes;and complication presented in 33.3%,pneumothorax,bleeding,hemoptysis and hypodermic haematoma were 17.0%,7.9%,7.3%and 1.2%,respectively.Conclusion The technique of pulmonary percutaneous biopsy under CT guided is a perfect method for qualitative diagnosis of difficult pulmonary pathological changes:it is of great clinical application.

The membrane fluidity of mitochondria isolated from langendorff perfused rat hearts was studied with ESR spin labeling technique and TBA method was used to detect the content of lipid peroxide. After total ischemia for 40min followed by a 20min reperfusion the 10-DSA region of the mitochondrial membrane showed significantly increased S (0.1(?)2?0.006) and ?_c(7.13?0.09?10~(10)sec) values (i. e. a decrease of membrane fluidity)in comparison with aerobic control (S=0.103?0.007); ?_c=6.86?0.20?10~(-10) sec) (P0.05). In addition, we found that DS182 could remove O_2~? produced by O_2~? generating system. Our results have showed that DS-182 is a potent scavenger of superoxide. The protective effect of DS-182 on mitochondria during ischemia/reperfusion might be due to its antioxidative activity。