A simpler method for preparing hap- toglobin from the waste residue of Rivanol- ammonium sulphate fractionation of human serum during the production of human immunoglobulin is described. The agar do- uble diffusion test shows that the large amount of haptoglobin can be prepared by this procedure and the immunoelectropho- resis analysis demonstrated specific preci- pitated line with the reference anti-hapto- globin antibody, showing that the protein molecule of haptoglobin is homogeneous.

Objective To discuss progress in Qinghai Tibetan Thymidylate synthase enhancer in patients with gastric cancer (TSER),ERCC1 polymorphism of genes,gene expression and mRNA studies.Methods 210 cases of advanced gastric cancer were enrolled as required to complete the treatment and follow-up.TSER,ERCC 1 gene polymorphism were tested using PCR-RFLP method.Analysis of gastric cancer is based on the results of gene polymorphism and clinical pathological factors in relations.The expression of ERCC1 mRNA was detected by RTPCR,and tracking access after treatment.Analyzing the relationship among ERCC1 mRNA expression,clinical treatment of overall survival,total response rates and gene polymorphism.Results In patients with advanced gastric C/C in Qinghai ERCC1 gene frequency was 54.2％,C/T frequency was 42.7％,T/T ERCC1 gene frequency was 5.6％.ERCC1 mRNA expression and clinical effect in the treatment of pathological factors were not related,ERCC1 mRNA and gene ERCC1 polymorphism had no relevance.Conclusions Progress in Qinghai Tibetan TSER gene is highly expressed type 3R/3R in patients with gastric cancer.ERCC 1 gene polymorphisms with type C/ C is overexpressed.ERCC1,TSER gene polymorphism and clinicopathologic parameters in advanced gastric is not related.Extension of Tibetan ERCC1 mRNA in gastric cancer patients expression has no association with ERCC1 gene polymorphism.

Hepatocellular carcinoma (HCC) is one of the most common malignancies in China, which serious threat to life and health. Surgical treatment of HCC is one of the most important therapeutic options for long-term survival of HCC patients, including hepatectomy and liver transplantation. Hepatic arterial infusion chemotherapy (HAIC) infused the therapeutic agents directly into the hepatic artery, the main blood supply in HCC, providing higher concentration and reaction rate. HAIC is an effective treatment for hepatectomy of downstaging and adjuvant therapy, which can improve the feasibility of hepatectomy and decrease the recurrence rates after resection. In this paper, we will review the applications and advances of HAIC in surgical treatment of HCC.

Objective To evaluate the value of ultrasonography in the diagnosis of special ectopic pregnancy(SEP).Methods Ultrasonography was performed in 31 patients with SEP.The sonographic features of two-dimensional ultrasound and color Doppler of SEP were analyzed and the sonographic results were correlated with surgical and pathologic findings. Results Ultrasonography accurately diagnosed interstital tubal pregnancy in 21 cases,cervical pregnancy in 4,isthmus pregnancy in 2,rudimentary horn pregnancy in 1,and 3 cases were misdiagnosed,thus,the diagnostic accuracy of ultrasound for SPE was 90.3% .Conclusions Ultrasonography has an important role in diagnosing SEP.

Objective To observe leptin level before and after rosiglitazone administration and to explore the possible mechanism of rosiglitazone's improving insulin sensitivity.Methods A total of 30 patients newly diagnosed with type 2 diabetes were separated into three groups at random,and treated with rosiglitazone(4mg/d),metform(750mg/d) and gliclazide(160mg/d),respectively.Serum leptin,insulin and GHbA1c were measured after 12 weeks.Results After 12 weeks' treatment,the fasting glucose and GHbA1c in each group declined sharply,while free insulin did not change.The leptin level in rosiglitazone group decreased and insulin sensitivity was improved after administration,but there was no change in metformin and sulfonylureas groups.Conclusion Leptin may play a role in rosiglitazone's mediating the insulin-sensitizing effects.

Objective To investigate the effect of opiate receptor antagonist naloxone on the electroencephalographic (EEG) power spectrum in morphine-addicted rats.Methods 20 male adult Wistar rats were alternately injected with morphine (Mf, 10 mg/kg,sc.;Mf withdrawal (Mf-Wd) group, n=10) or saline (Sal, 1 ml/kg, sc.;Sal-Wd group, n=10) for 8 days to establish Mf physical dependent model.Withdrawal scores were evaluated by naloxone-precipitated withdrawl signs (5 mg/kg,ip.).Another 20 rats were divided into Sal-CPP group and Mf-CPP group with 10 rats in each group.Rats in the sal-CPP group were injected with saline once a day for 8 days while rats in the Mf-CPP group were injected with saline and morphine alternatively once a day for 8 days.The EEG signals were recorded from their dorsal hippocampi before and after naloxone injection under anesthesia;and the EEG power data (1 ～ 100 Hz) were analyzed.Results (1) Compared with Sal-Wd group (22.69± 3.59), the Mf-Wd group showed a higher withdrawal scores after naloxone injection(84.90± 13.40, P＜0.01).(2) The CPP scores of Mf-CPP rats (80.69± 14.96) were significantly higher than the baseline (45.29± 15.79) and the Sal-CPP rats (41.84± 12.47;all P＜0.01).(3)After naloxone injection,the EEG powers of δ band and γ band of Mf-CPP rats decreased temporarily c ompared with their baseline (δ band: (81.80±30.91) mV2 · Hz vs (112.49±39.43) mV2 · Hz;γ band:(25.35±11.20)mV2 · Hzvs (28.71±13.06)mV2 · Hz;allP＜0.05).Conclusion Opiate receptor antagonist naloxone can decrease the hippocampal EEG powers of δ band and γband in Mf-addicted rats.

BACKGROUND:To explore the methods for secondary limb revascularization after endovascular repair is one of the most urgent research topics.
OBJECTIVE:To investigate the biological mechanism about the transplantation of autologous peripheral blood stem cel s to lead and perfect the effect of ischemic tissue for secondary revascularization.
METHODS:Forty-two patients with critical low limb ischemia were selected and treated with endovascular repair as first revascularization and transplantation of autologous peripheral blood stem cel s as secondary revascularization. The secondary revascularization was carried out at 3 months after the first blood flow remodeling. The patients receiving secondary revascularization were fol owed for 4 years.
RESULTS AND CONCLUSION:After transplantation of autologous peripheral blood stem cel s, the patients complained that the rest pain of the lower leg was relieved obviously and intermittent claudication distance was significantly lengthened. Limb salvage rate was 100%. Skin temperature index was 1.6±0.3, transcutaneous oxygen pressure was (5.00±1.26) kPa, ankle-brachium index was 0.9±0.2, photoplethysmograpy index was 0.8±0.1, saturation of blood oxygen was (79.4±20.4)%, and digital subtraction angiography score was (1.3±0.2)
points. After transplantation, local blood flow indicators of the low limbs were positively correlated to local symptom indicators of the limbs (0.6

Objective To appraise facial skin's quality conveniently and correctly.Methods The quality appraisal model for facial skin image was established based on improved BP nerve network algorithm,and the model was trained with the intrinsic pattern of the training sample,then the appraisal result was output after inputting indexes on the skin.Results For 23 sets of samples,the appraisal accuracy reached 95%.Conclusion The network model,with a high accuracy and a low error rate,is applicable to facial skin image quality appraisal.

Objective To investigate the effect of methylprednisolone on the expression of glial fibrillary acidic protein (GFAP) and proliferating cell nuclear antigen (PCNA) in M?ller cells of rats' retinae injured by laser. Methods Forty SD rats were randomly divided into two groups and inflicted with laser photocoagulation.The rats in treatment group were given methylprednisolone by intraperitoneal injection with a dose of 30 mg/kg for 3 days.At the 3rd,7th,14th,and 28th day after photocoagulation respectively, the eyes were enucleated,fixed and cut into sections.Immunohistochemical examination was used to detect the expression of PCNA and GFAP. Results After photocoagulation the M?ller cells expressed PCNA both in the treatment and control group,and the expression of PCNA decreased sharply after 3 days. The expression of PCNA in treatment group was less than that in control group. After photocoagulation the M?ller cells also expressed GFAP and the expression of GFAP lasted for at least 28 days ,and the expression of GFAP expression in the treatment group was less than that in the control group. Conclusion Methylprednisolone can reduce the expression of GFAP and PCNA in M?ller cells of rats' retinae injured by laser.

The interventional treatment of lung cancer is tightly related with hemodynamic situation of artery.To study hemodynamics of the interventional treatment of lung cancer can significantly improve curative effect and decrease complications.Through simulation experiment,the ratio of drug injection around the entrance of bronchial artery is obtained and the definition of natural perfusion region is given.The evidence for interventional treatment of lung cancer and the principle avoiding high-level paraplegia in clinical interventional treatment of lung cancer are also presented.