Folk therapeutics is characterized by using manipulation techniques or tools to allieviate pain. Because of its convenience for operation and rapid effectiveness, the folk therapeutics is handed down from mouth to mouth and lasting for long time. This article, from a medical history view, briefly introduced the developing process of massage, cupping, thermalism, chiropractics, guiding and so on, to make certain a positive position of this therapeutics in the history if TCM external treatments .

Objective Using hypothyroid,hyperthyroid and normol control rats model,we investigated mRNA expression of the nerve-growth-factor and its receptor TrkA in hippocampus and parietal cortex at 14,21,28days of ages,to offer a possible neurobiological explanation about mechanism of thyroid hormone modulating NGF and its receptor TrkA.Methods For hypothyroid group,rats were reared with 0.02% methimazol water from 5~6 day of gestation to the day when they were sacrificed;for hyperthyroid group,rats were daily intraperitoneal injected of 0.4?g/g body weight tiiodothyronin from beginning of delivery;for normal control rats were reared a normol diet and water.NGF mRNA and TrkAmRNA were measured by in site hybridization.Results The peak of mRNA expression of NGF and TrkA is at P21and P28 respectively,.The mRNA expression of NGF and TrkA in hypothyroid rats was significantly lower than that in age-matched euthyroid at P14,P21,P28(P

Urotensin Ⅱ,a cycle peptide originally found from the fish,is the most potent vasoconstrictor.Urotensin Ⅱand its receptor have been found in central nerve system,cardiovascular system and other organs and tissues.It has been suggested that urotensin Ⅱplays important roles in the physiological and pathological procedures.Based on the evidences obtained from experiments,urotensin Ⅱmay become a new target for treating many diseases,particularly for cardiovascular diseases.

Objective To compare the comprehension monitoring in reading between students with and without hearing impairment. Methods An error detection paradigm and an eye-movement recordings method were used in two samples of hearing impairment students and no-hearing impairment students with passages that contained inconsistencies with interval 2 sentences, 4 sentences, 6 sentences and 8 sentences. Results The scores of reading comprehension, the index of tradition monitoring and the index of eye movement were lower in the hearing impairment students than in the no-hearing impairment students (P<0.05). Conclusion The comprehension monitoring ability in reading is poor in the hearing impairment students.

Objective Changes of thyroid stimulating antibody(TSAb) and thyroid stimulating blocking antibody(TSBAb) in the treatment of anti-thyroid drugs(ATDs), and the effect of ATDs combining with levothyrocine(LT4) on TSAb and TSBAb were analyzed. Methods Using recombinant Trxfus. TSHRn protein and Trxfus. TSHRc protein as antigens, and TSH receptor antibody(TRAb)-N(TSAb binding hot spots), TRAb-C(TSBAb binding hot spots)in the serum of thyroid disease patients were measured with ELISA. The changes of TRAb-N, TRAb-C over 36 months in 117 TRAb-N positive Graves′ patients with hyperthyroidism were analyzed retrospectively. In the course of treatment, 41 cases as A group with ATDs and LT4 treatment, 76 cases as B group with only ATDs, The changes of TRAb-N and TRAb-C were observed in the two groups. Results (1)According to the change of TRAb-N, 117 TRAb-N positive Graves′ patients with hyperthyroidism were different. In group Ⅰ, 10 patients continued to have persistently positive TSAb and continued to have hyperthyroidism, remission rate 0%. In group Ⅱ, 17 patients showed complicated TRAb-N changes, 12 of 17 patients got relapse, 5 of 17 patients got remission, remission rate 29.4%. And in group Ⅲ, with TRAb-N dropping gradually, 15 of 89 patients got relapse, 74 of the 89 patients got remission, remission rate 83.1%. Three groups were significantly different with x2 test(P<0.01). One of the 117 TRAb-N positive Graves′ patients with hyperthyroidism developed TRAb-C positive hypothyroidism. (2)According to combining with and without LT4 during the treatment of ATDs,the patients were divided into 2 groups(Group A: ATDs combined with LT4; Group B: only ATDs). These 2 groups were significantly different in TRAb-N at baseline and 3 months(P<0.01), TRAb-C between two groups were not significantly different in all times(P>0.05). Conclusion TSAb and TSBAb can be used to document TRAb-function, which is significant for us to predict the changes of thyroid function. During ATDs treatment, the temporary early low-dose application of LT4 did not significantly affect TSAb and TSBAb.

Objective To select and express a human thyrotrophin receptor antibody (TRAb) Fab fragment from phage antibody library constructed with phage display technology. Methods With immobilized antigen, the reconstructed humanized TRAb Fab library was enriched by five rounds panning (adsorption-elution-amplification). The TSAb Fab and TBAb Fab fragment were selected by coated fusion proteins of hTSHRn and hTSHRc. The positive clones were identified and selected by Phage-ELISA. TRAb positive clones were identified by PCR and double restriction enzyme digestion. The soluble TRAb (TSAb, TBAb) Fab fragments were expressed. TRAb (TSAb, TBAb) Fab fragments were identified by Western blotting assay. The DNA fragment was sequenced from the positive clones. Results Following five rounds of biopanning, TRAb (TSAb,TBAb) Fab phage antibody was screened. The enrichment effect reached to 77 times and 94 times. The soluble TRAb (TSAb,TBAb) Fab antibodies were expressed from positive clones and identified by phage ELISA. Western blotting analysis showed that the phage displaying Fab had significant binding activity with antigens. These sequence analysis showed that all of the heavy chain Fd gene and light chain gene were derived from human immunoglobulin variable region. The light chain variable region of the monoclonal 48 was homologous to the immunoglobulin light chain Vλ homology of 94.4%, and the heavy chain variable region of the monoclonal 48 was homologous to the immunoglobulin heavy Fd chain VH4 homology of 88.9%. The light chain variable region of the monoclonal 56 was homologous to the immunoglobulin light chain Vλ homology of 95.6%, and the heavy chain variable region of the monoclonal 56 was homologous to the immunoglobulin heavy Fd chain VH3 homology of 84.6%. Conclusion We have successfully selected TRAb (TSAb, TBAb) Fab fragment from a human phage display immune antibody library.

Objective To inverstigate the efficiency and safety of transcatheter uterine artery embolization (TUAE) as well as the pathological changes and it′s significance after embolization. Methods 44 cases of uterine fibroids were treated by TUAE using lipiodol pingyangmycin emulsion (LPE) and Gelfoam sponge paeticles. Seven of 44 cases underwent hysterectomy or myomectomy from 1 to 6 weeks after TUAE.The specimens were studied to observe pathological changes of fibroids and myometrium. 37 of 44 patients were followed up for 1 to 6 months to observe the changes of fibroid size and uterine volume. The changes of ovarian function and myometrium after TUAE were found out by observation on mensis, test of ovarian hormone, premenstrual diagnostic curettage and pathology of the resected specimens. Results Total improvement of symptams occurred in 91.9%(34/37), including control of menorrhagia in 91.4%(32/35), disappearance of lumbago and lower abdominal pain in 85 3%(29/34),relief of freguency and urgency of micturition in 50.0%(7/14). Average decrease of fibroid and uterine volume at 6 months after TUAE was 61.3% and 50.6% respectively. No amenorrhea happened. Premenstrual diagnostic curettage showed secretory endomtrium. There was no significant difference in ovarian hormon level befor and after embolization. Pathological study showed that lipiodol was found to deposit in fibroids and was not seen in myometrium. Spotty necrosis 2 weeks occurred in fibroid and extensive patchy necrosis 3 weeks after embolization. The necrotic area became larger with elapse of time postoperative. Necrosis was not seen in myometrium. Conclusion TUAE is a new ,and effective procedure for uterine fibroid and LPE as an embolizing agent is safe without damage on myometrium.

Objective To compare the effect of electrode positions o n multifocal visual evoked potential(mf-VEP). Methods Ten healt hy individuals were tested with RETIscan multifocal VEP system 3.20, each indiv idual was tested with four electrode positions: the active and reference electro de were placed 2.0 cm above and below the inion in Ch1;3.0 cm above and 4.5 cm below the inion in Ch2;2.0 cm on both right and left side of the inion in C h3 and 4.0 cm on both right and left side of the inion in Ch4. The summed ampli tudes of hemifield mf-VEP were analyzed and compared according to different ele ctrode positions. Results The difference among summed amplitud es of the upper or lower hemifield mf-VEP recorded with Ch1、Ch2、Ch3 and Ch4 w as statistically significant;and the difference between Ch2 and the other chann els was statistically significant too. The difference among summed amplitudes of the horizontal sites whose amplitudes were small when recorded with Ch2 and sum med amplitudes of the corresponding sites recorded with Ch3 and Ch4 were statist ically significant;and the difference between Ch4 and the other channels was st atistically significant too. Conclusions compared to Ch1、Ch3 and Ch4, Ch2 can make a better recording of mf-VEP. As for some sites, especi ally those along horizontal line, horizontal electrodes could improve the amplit udes of mf-VEP recorded with vertical electrodes,and Ch4 could do better to im prove the mf-VEP recorded with Ch2 than Ch3.

To screen potential hamster chymase 2 inhibitors, a high-throughput screening (HTS) model was established. Recombinant hamster chymase 2 with active form was cloned and expressed in E. coli. The HTS model with total volume of 50 microL in 384-well microplate was based on fluorescence analysis and was proved sensitive as well as specific (Z' = 0.84). A total of 40 080 samples (including 28 060 compounds and 12 020 natural products) were screened, and 613 samples with inhibition greater than 90% were selected for further rescreening. Finally, compounds J16647 and J16648 were identified with high inhibitory activity on chymase 2, and whose IC50 values were 0.823 and 0.690 micromol x L(-1), respectively.

ObjectiveTo observe the changes in TSH receptor antibody (TRAb) and soluble intercellular adhesion molecule-1 ( sICAM-1 ) levels after treatment of propylthiouracil ( PTU ) and methimazole( MMI ) in patients with Graves' disease (GD). MethodsOne hundred and six cases of clinically diagnosed patients with GD were divided into PTU and MMI groups( each group, n =53 ). The patients in two groups were regularly followed for 36 months. TRAb and sICAM-1 were measured with ELISA method. Results( 1 ) The general data of two groups were not significantly different before treatment( all P＞0. 05 ). ( 2 ) There was no difference in TRAb positive rates between two groups before treatment. The clinical remission rates between PTU and MMI groups showed no difference (83.02％ vs88.68％ ). The cure rate was higher in MMI group than in PTU group( 58.49％ vs 37.74％, P＜ 0. 05 ). (3) There existed significant differences in TRAb levels determined before and 6, 12, 24,30, and 36 months after treatment( all P＜0. 01 ), being gradually decreased with time( F=275.48 ,P＜0.01 ). TRAb levels between two groups were significantly different( F=5.86, P＜0. 05 ). (4) sICAM-1 levels at 36 months after treatment compared with the baseline in both groups were statistically different (P＜0. 01 ), but no difference was found between two groups.ConclusionsBoth PTU and MMI improve the immune status of patients with GD,and the immunosuppressive effect of methimazole is more evident.