Objective Primary myocardial cell culture , as an important technique for in vitro experiment , provides an essen-tial basis for the establishment of models of cardiovascular diseases .This study aimed to investigate the influence of digestion time on primary myocardial cell culture and to find some simple primary culture methods for obtaining myocardial cells with high survival rate , purity, and activity. Methods We used only one collagenase for repeated digestion of myocardial tissue for 8 and 10 minutes, re-spectively , and obtained high-purity cardiomyocytes by differential adhesion and Brdu inhibition .We observed the morphological chan-ges of the cardiomyocytes under the optical microscope , measured their vitality according to the beating frequency in different regions , and counted the living cells after typan blue staining. Finally, we established a model of myocyte hypertrophy for verification of cell activity and compared the cell surface area between the experimental ( angiotensinⅡ) and control ( 10%FBS DMEM [H +] medium ) groups. Results After cultured for 48 hours, the primary cardimyocytes in the 10 min group mostly extended pseudopodia , in-terwoven into a network and gradually forming cell clusters , with obviously powerful beating and contraction .After isolation and purifi-cation, the average survival rate of the cells was >90%, significantly higher in the 10 min group than in the 8 min ([95.4 ±0.8]%vs [93.0 ±0.8]%, P<0.05).The positive expression rate of cardiac α-actin was >95%in both of the groups.After cultured with angiotensinⅡfor 48 hours, the 10 min group showed a significantly increased level of atrial natriuretic peptide (ANP) (20.8 ±0.67 vs 15.8 ±0.48, P<0.05) and cell surface area ([34.77 ±8.43] μm2 vs [14.11 ±4.29] μm2, P<0.05) as compared with the 8 min group. Conclusion Primary culture of cardimyocytes with 8 min digestion is simple and time-saving, and what is most important , can obtain high-quality cardiomyocytes .

Objective:To analysis the relationship of cephalometric measurements from intercuspal position(before and after treat-ment)and retruded contact position(before treatment).Methods:18 cases with Class III malocclusion were treated using MBT straight wire appliance technique,all the incisal relationships could be edge-to-edge when the mandible was retruded,X-ray films of intercus-pal position and retruded contact position before treatment and intercuspal position after treatment were taken.The cephalometric meas-urements were statistically analysed by ANOVA.Results:After 25 months treatment the anterior crossbite were corrected in all cases. The molar relationship were class Ⅰ.The measurements of SNB,ANB,MP-FH,MP-SN,U1-SN,L1-MP,Y-axis angle and ANS-Me were significantly changed.Conclusion:After the treatment of Class III malocclusion by MBT straight wire appliance,the sagittal and vertical mandibular location can be between intercuspal position and retruded contact position of pretreatment,and it is more close to the retruded contact position.

Objective:To investigate the iodine nutrition level of pregnant women in Shanxi Province, and to provide basis for guiding pregnant women to supplement iodine scientifically.Methods:From 2016 to 2018, 40, 80 and 118 counties were selected in Shanxi Province. In each county, 5 townships were selected according to the east, west, south, north and middle orientation, 20 pregnant women were selected from each township. Urine and salt samples of pregnant women were collected, and urinary iodine and salt iodine contents were determined, respectively.Results:From 2016 to 2018, 3 590, 7 907, and 11 750 salt samples were collected from pregnant women's homes. The medians salt iodine were 23.80, 23.70, 23.25 mg/kg, and the qualified iodized salt consumption rates were 91.06% (3 269/3 590), 90.06% (7 121/7 907), 92.21% (10 835/11 750), and the coverage rate of iodized salts were 97.72% (3 508/3 590), 97.00% (7 670/7 907), 98.53% (11 577/11 750), and the qualified rate of iodized salts was 93.19% (3 269/3 508), 92.84% (7 121/7 670), 93.59% (10 835/11 577), respectively. There were statistically significant differences in the median salt iodine and qualified iodized salt consumption rate among pregnant women in different years ( H = 99.915, χ 2 = 27.988, P < 0.05). Totally 3 902, 7 892 and 11 745 urine samples of pregnant women were tested, and the medians (quartiles) urinary iodine were 174.20 (114.00, 251.08), 180.70 (117.13, 258.58) and 179.40 (115.90, 249.00) μg/L, respectively. There was statistically significant difference in the median urinary iodine among pregnant women in different years ( H = 12.368, P < 0.05). From 2016 to 2018, counties with appropriate levels of iodine accounted for 70.00% (28/40), 73.75% (59/80) and 70.34% (83/118), respectively. The medians (quartiles) urinary iodine levels of pregnant women in the early, middle and late stages of pregnancy ( n = 2 225, 5 727, 3 793) in 2018 were 177.62 (117.28, 257.23), 178.21 (117.40, 248.40) and 172.70 (112.98, 245.70) μg/L, respectively, and there was statistical by significant difference in urinary iodine levels among pregnant women in different pregnancy periods ( H = 11.077, P < 0.05). Conclusions:From 2016 to 2018, the iodine nutrition level of pregnant women in Shanxi Province is generally in an appropriate state, iodine deficiency or more than appropriate still exists in some areas. We should continue to carry out monitoring work, and focus on strengthening health education for pregnant women, and guide them to supplement iodine scientifically and accurately.

Objective To evaluate the effect of air pollution on carbonylated protein level in the stratum corneum,and to assess the protective effect of pink pepper tree extracts and lipid mixtures on skin damage.Methods After the investigation of influencing factors in the preliminary experiment,fluorescence labelling assay was performed to detect the carbonylated protein level in the skin stratum corneum at different body sites of 34 healthy testees.Cigarette smoke was used to simulate pollutants,and the forearms of 15 healthy testees were exposed in the customized pollution simulation chamber with the flexor aspects facing upwards.After 0,1,2,4,5 hours of exposure,stratum corneum samples were collected by using D-squame tapes.In each of 14 selected healthy testees,3 adjacent areas on the flexor aspect of unilateral forearm were divided into 3 groups:pink pepper tree group treated with 1％ aqueous solution of pink pepper tree extracts,control group treated with deionized water,and blank group receiving no treatment.Then,the forearms of the 14 testees were exposed in the pollution simulation chamber for 5 hours,and stratum corneum samples were collected from the 3 areas before and after the exposure.Another 16 healthy testees were included,and 3 adjacent areas on the flexor aspect of their unilateral forearms were divided into 3 groups:lipid mixture group treated with 5％ lipid mixture emulsion,control group treated with lipid mixture-free placebo emulsion,and blank group receiving no treatment.Then,the forearms of the 16 testees were exposed in the pollution simulation chamber for 5 hours,and stratum corneum samples were collected from the 3 areas before and after the exposure.Moreover,twenty healthy testees were enrolled into the double-blind split-face clinical trial.That is,one half of the face was randomly chosen to be treated with 1％ emulsion of pink pepper tree extracts,and the other facial side was treated with placebo emulsion.Before and after 56-day treatment,stratum corneum samples were collected from the cheeks of testees by using D-squame tapes.Fluorescence labelling assay was conducted to detect the carbonylated protein level in the above stratum corneum samples.Results The analysis of 34 testees showed that carbonylated protein levels (average fluorescence intensity) significantly differed among different body sites (P ＜0.001),and the carbonylated protein levels were significantly higher in the cheeks (26.3 ± 7.1) and forehead (22.9 ± 7.9) than in the forearm (14.7 ± 4.9) and waist and back (12.6 ± 4.2) (P ＜ 0.001),and higher in the forearm than in the waist and back (P =0.046).In the short-term simulated accelerated exposure experiment,the carbonylated protein level increased along with the increase of the duration of exposure to pollution (R2 =0.995 9).After 5-hour exposure,the pink pepper tree group and lipid mixture group both showed significantly lower elevated levels of carbonylated protein in the stratum corneum (9.7 ± 5.2,5.8 ± 4.9) compared with the corresponding blank groups (19.0 ± 10.0,17.4 ± 8.8;P ＜ 0.005) and control groups (18.5 ± 7.3,15.9 ± 6.4;P ＜ 0.005).In the long-term human trial,the carbonylated protein levels significantly decreased in the facial side treated with 1％ emulsion of pink pepper tree extracts for 8 weeks compared with the placebo-treated facial side.Conclusion Air pollution aggravates skin damage induced by protein carbonylation in the stratum corneum,and pink pepper tree extracts and lipid mixtures can effectively reduce the carbonylated protein level.

Objective To calculate the electric field intensity and transmembrane voltage of spherical cells and ellipsoi -dal red blood cells ( RBC) in an extremely low frequency electromagnetic field .Through this calculation , we can provide reference to the search for interaction targets and mechanics between the extremely low frequency electromagnetic field and organisms.Methods The Finite Element Method was used in the numerical computation for the spherical cell model and the ellipsoidal RBC model .Results The electric field intensity of the two types of cells on the cellular membrane was both significantly higher than the applied electric field strength , and the values of the induced field strength and transmembrane voltage varied with the direction of the electric field periodically .Conclusion The cell shape and direction of the applied electric field are not the main determinants of the cellular membrane electric field intensity and the transmembrane voltage compared with electromagnetic parameters .The distribution of the electric field intensity and transmembrane voltage are re-lated to the direction of the applied electric field.

Objective To observe the effect of repetitive focal muscle stimulation on recovery of upper limb function after stroke.Methods From October, 2016 to February, 2018, 46 stroke patients at early stage were recruited and divided into control group (n=23) and observation group (n=23). Both groups received basic rehabilitation training. The observation group accepted additional repetitive focal muscle stimulation on palms, forearm extensor carpi radialis, biceps brachii and muscles around scapula. The control group received sound stimulation as placebo treatment. The treatment was implemented once a day, five days a week for four weeks totally. The scores of Fugl-Meyer Assessment -Upper Extremities (FMA-UE), the Motricity Index of Upper Extremities (MI-UE) and the Hong Kong version of Functional Test for the Hemiplegic Upper Extremity (FTHUE-HK) were compared before and four weeks after treatment. Results After treatment, the scores of FMA-UE, MI-UE and FTHUE-HK improved in both groups (F>2.577, P<0.05), and were higher in the observation group than in the control group (t>2.153, P<0.05).Conclusion Repetitive focal muscle stimulation could facilitate to recover the function of upper limb in early-stage stroke patients.

Objective To explore the research hotspots and development trends of Ziziphi Spinosae Semen in the past 20 years,and to provide reference for related research.Methods Literatures on Ziziphi Spinosae Semen were searched from January 1,2000 to December 31,2022 in CNKI,Web of Science Core Collection Database.VOS viewer software was used to visually analyze the citation frequency,research institutions and keyword hotspots of English literatures.CiteSpace software was used to visually analyze research institutions,authors,emergence keywords and keyword overlap time of Chinese literatures,and Microsoft Excel 2021 software was used to analyze of annual publication trends and publication volume of Chinese and English literatures,and download frequency of Chinese literatures.Results A total of 4 872 Chinese and 128 English literatures were included,with an overall upward trend in the number of annual publications.The research institutions with the highest number of publications in Chinese and English were Shandong University of Traditional Chinese Medicine and Tianjin University of Commerce,and the authors with the highest number of publications were DU Chenhui and XIE Junbo,respectively.The most frequent keywords in Chinese literatures were"Ziziphi Spinosae Semen","composed"and"application of compound therapy",and in English literatures were"performance","oxidative stress".Conclusion From 2000 to 2022,the research hotspots of Ziziphi Spinosae Semen mainly focused on the chemical composition,pharmacological effects and clinical application analysis,compatibility research,formulation and preparation.Quality control and evaluation of Ziziphi Spinosae Semen,and the research on the mechanism of preventing and treating insomnia with Ziziphi Spinosae Semen may become future research directions.

Objective To explore the feasibility and safety of carotid artery stenting (CAS) via right transradial approach (TRA).Methods A retrospective analysis was made on 46 cases undergoing the CAS via right TRA.Patients were divided into the right carotid artery group (RCA),bovine left carotid artery group (B-LCA),nonbovine carotid artery group (NB-LCA) according to the lesion location.Low TRA or high TRA were selected to overcome the difficulties of insufficient supporting power,using techniques such as catheter looping and retrograde engagement technique(CLRET).Results CAS were successful in all cases (100％),the differences of the operation time and fluoroscopy time among RCA group,B-LCA group and NB-LCA group were not significant.In NB-LCA group,CLRET were applied in 10 cases (55.56％,10/18),includeing 8 cases with type Ⅲ aortic arch(100％,8/8)and 2 cases with type Ⅱ aortic arch (33.33％,2/6).The CLRET prolonged the operation time and fluoroscopy time [(39.45 ±7.27) min vs.(30.80±4.66) min;(11.84 ± 2.05) min vs.(9.91 ± 1.45) min,P ＜0.05).There was no cerebrovascular events and puncture point complications in perioperative period.Conclusion Right transradial approach for carotid artery stenting is safe and technically feasible,especially in RCA stenosis and LCA stenosis with type Ⅰ or Ⅱ aortic arch.

Objective:To explore the psychological resilience of adolescent leukemia survivors and analyze its influencing factors.Methods:From October 2018 to August 2020, convenience sampling was used to select adolescent leukemia survivors in the routine follow-up group and follow-up group for children with leukemia who stopped taking medication in Beijing Children's Hospital affiliated to Capital Medical University as the research object. The General Information Questionnaire, Resilience Scale for Chinese Adolescents, and the General Self-Efficacy Scale were used to investigate adolescent leukemia survivors. A total of 110 questionnaires were issued, and 106 valid questionnaires were returned, with the effective response rate of 96.36%.Results:Among 106 adolescent leukemia survivors, the total score of psychological resilience was (97.83±14.93) . The results of multiple linear regression analysis showed that disease risk, duration of drug withdrawal, and general self-efficacy were the influencing factors of psychological resilience in adolescent leukemia survivors, and the difference was statistically significant ( P<0.05) . Conclusions:The psychological resilience level of adolescent leukemia survivors is at an upper-middle level. Medical and nursing staff, the family and friends of adolescent leukemia survivors should pay active attention to survivors whose disease risk is medium or high risk or whose duration of drug withdrawal is less than 5 years, and provide them with a lot of support and help. At the same time, pay attention to the general self-efficacy of survivors, provide targeted self-care guidance or substantive help for the survivors to return to school and society so as to improve their psychological resilience.

Ziziphi spinosae semen mainly contains contents of saponins,flavonoids,alkaloids and aliphatic acids.Meanwhile,it has a variety of activities such as sedative-hypnotic,anti-anxiety,anti-depression,nerve protection,cardiovascular and cerebrovascular protection,liver protection,and antioxidant,which is widely used in medicine,food,health food and other fields.The chemical constituents,pharmacological action and clinical application of Ziziphi spinosae semen were systematically summarized in this paper by reviewing the literature,in order to provide theoretical guidance for the sustainable development of the resources and the rational use of Ziziphi spinosae semen.