OBJECTIVE:To analyze problems occurred in the practical use of clinical medicine cabinet and raise countermeasures.METHODS:The application data of drugs in clinical medicine cabinet were analyzed with the help of Hospital Information System(HIS).RESULTS:Low application frequency and unclear medicine sign were the common problems occurred in the practice of clinical medicine cabinet management;furthermore,the storing condition remained to be improved.CONCLU-SION:Management of clinical medicine cabinet should be regulated and strengthened to promote safe and effective drug use in the clinic.

Objective To investigate the significance of I?B kinase-?(IKK-?) and the protective effect of Ligustrazini(Lig) in acute lung injury(ALI) induced by hemorrhagic shock(HS) in rabbits. Methods Thirty rabbits were randomly divided into three groups: HS plus endotoxin group(n=10), Lig treatment group(n=10) and the normal group(n=10). In situ hybridization and immunohitochemistry combined with in situ quantitative analysis were used to detect the distribution and relative contents of IKK-? and NF-?B in lung tissues. The plasma concentration of tumor necrosis factor-?(TNF-?) was measured by ELISA. Morphological change of the lung was observed by light microscopy. Results Compared with the normal group, the expression levels of IKK-?(0.223?0.080), NF-?B(0.162?0.021), and TNF-?(809.33?201.6) were obviously increased in HS plus endotoxin group(P

Objective To investigate the changes and significance of I?B kinase-?(I?K-?) in the lung tissues of rabbits with hemorrhagic shock.Methods The expressions of I?K-? and NF-?B in the lung tissues and the concentration of tumor necrosis factor-?(TNF-?) in the plasma were measured by in situ hybridization(ISH),immunohitochemistry and enzyme linked immune adsorbent analysis(ELISA), respectively. And the pathological changes were examined with light microscope in lung tissues.Results In hemorrhagic shock group,the expressions of I?K-?(0 1685?0 0164)and NF-?B( 0 1469?0 0083)in lung tissues , the level of TNF-?(636 72?100 23) in the plasma were obviously higher than those of normal group [I?K-?(0 0427?0 0241),NF-?B(0 0358?0 0048),TNF-?(199 51?35 69)ng/L](all P

Objective To investigate the preventive mechanism of Anisodamine (654-2) on multiple organ dysfunction syndrome (MODS) of rabbits. Methods Rabbit model of MODS induced by hemorrhagic shock and endotoxin was used in this study. Twenty-four rabbits were randomly divided into the control group (C group) , hemorrhagic shock plus endotoxin group (M group) and 654-2 treatment group (T group). The expression of IKK-? of pulmonary alveolar macrophage (PAM) and kuffer cell(KC), the NF-?B activity of nuclear protein extracted from PAM and KC and the concentration of tumor necrosis factor-? (TNF-?) in the culture supernatant were measured by in situ hybridization (ISH), electrophoretic mobility shift assay (EMSA) and enzyme linked immune absorbent analysis(ELISA), respectively. Then the blood air, biochemical and pathological changes in visceral organs were examined in each groups. Results In PAM and KC of M group, The expression of IKK-? mRNA [(0 15?0 03);(0 17?0 04)], the activity of NF-?B [(1 49?0 30);(1 72?0 36)] and the secretion level of TNF-? [( 279 74?25 91);(300 05?30 86)ng/L] were significantly higher than those of control group (P

[Objective] To study the best skill of HP-?-CD containing Ezhu oil.[Method] Take Ezhu oil containting rate as appraisal index,use orthogonal testing method for technical selection.[Result] Apply water solution mix for inclusion,the gotten best technical conditions:under 60 ℃,Ezhu oil:HP-?-CD=1:30,the regulating rotating speed 600 r/min/5 h;the total score of inclusion rate and recepting rate was 90.97%.[Conclusion] The preparation technical impoves stability of Ezhu oil,with better optimization,and offering realiable theoretic foundation for industrial production.

Objective To observe effect of Guben Xiaoliu capsule combined with FOLFOX4 regimen in treating advanced colorect cancer patients. Methods 78 advanced colorect cancer patients were randomly assigned to treatment group (38 patients) and control group (40 patients). Oxaliplatin 85 mg/m2 iv infusion for 2 hours,d1. CF 200 mg/m2 iv infusion for 2 hours followed by 5-FU 400 mg/m2 iv infusion for 22 hours,d1-2. Every two weeks was a cycle. The control group was treated by FOLFOX4 regimen,while Guben Xiaoliu capsule was added in the treatment group. Patients were evaluated after 4 cycles. Results Clinical beneficial rate (CR+PR+SD) of treatment and contral group were 76.3% and 57.5% respectively (P

A metabonomics method based on gas chromatography_mass spectrometry ( GC_MS) was developed for detecting the significant differences of root exudates of the Cd hyperaccumulator Sedum alfredii under different treatments and study the effect mechanism of S. alfredii tolerating or accumulating the heavy metal Cd. The root exudates were collected after treatment for 4 and 8 days with 0 and 40μmol/L Cd. The collected solution was lyophilized and dissolved with methanol, and after derivatization with methoxyamine hydrochloride and N_methyl_N_trifluoroacetamide, the samples were analyzed by GC_MS. Principal component analysis ( PCA) and orthogonal partial least_squares discrimination analysis ( OPLS_DA) were carried out for pattern recognition and a clear separation among the different treatments was achieved. Twelve compounds which caused the separation among the different treatments were found and identified. With the change of treatments, the relative amount of these 12 compounds revealed different trends, which indicated that the Cd hyperaccumulator S. alfredii could adjust the secretion of root exudates to tolerate or accumulate the heavy metal Cd.

OBJECTIVE:To study the distribution characteristics of germacrone from zedoary turmeric oil (ZTO) in each tis-sue of mice,and to provide reference for further application of zedoary turmeric oil. METHODS:30 KM mice were given zedoary turmeric oil 0.5 mL;6 mice were randomly selected 1,2,4,8,12 h after medication,respectively. The contents of germacrone in heart,liver,spleen,lung and kidney tissues were determined by HPLC. 15 KM mice were selected,medication and sampling method were same as above;3 mice were collected at each time point respectively. The fluorescence intensity of germacrone in above sections were observed by fluorescence. The same number of mice were selected as control in 2 trials. RESULTS:The con-centration of germacrone in each tissue 1-4 h increased gradually as time and reached the peak value at 4 h. The contents of ger-macrone in liver and spleen were significantly higher than in heart and lung. The concentrations of germacrone in each tissue were ranked as liver>spleen>kidney>heart>lung. The results of fluorescence intensity observation was same as above results. CON-CLUSIONS:Results of 2 methods show same distribution characteristics of germacrone in mice tissues,and indicate that ger-macrone is distributed more in liver,spleen and kidney tissues and less in heart and lung.

OBJECTIVE:To provide reference for the improvement of management level in hospital central dispensing work.METHODS:By means of "Army No.1" system,drug stocktaking was performed based on goods section number.RESULTS & CONCLUSIONS:Due to "Army No.1" system,the target of accounts-goods conformity rate ≥90% has come off.Besides,the monthly inventory profits and losses main at a balance basically.This experience deserves to be popularized.