Objective To investigate the expressions of ?-catenin(?-cat)and ?-catenin(?-cat)and their relation with development and prognosis of non-small cell lung cancer(NSCLC).Methods Expressions of ?-cat and ?-cat were immunohistochemically studied in 43 specimens of NSCLC and 7 cases of normal lung tissues.Results Of the 43 NSCLC cases,the abnormal rate of ?-cat and ?-cat expression was 72.1% and 65.1%,respectively.Differentiation and lymph node involvement had a significantly positive correlation with abnormal expression of ?-cat or ?-cat(P

Objective To determine an optimal method of in vivo endothelialization when homograft valve (HV) leaflets are treated with different methods. Methods Twenty five fresh HV leaflets were divided into five groups randomly. The leaflets of GroupⅠ were fresh HV ones without any treatment; GroupⅡwere cryopreserved in liquid nitrogen for 1 month; Group Ⅲ was treated by DOA; GroupⅣ was treated by 25 g?L -1 glutaraldehyde; Group Ⅴ was first treated by hypotonic/hypertonic, then with an enzyme-based solution. Each HV leaflet was trimmed to 2 mm one which was cultured with lymphocyte from the recipient. In order to compare the antigenicity of HV leaflets, 3H-TdR incorporation was observed after tissue-cell coculture and stimulous index (SI) was calculated. The HV leaflets of different groups were implanted into the sheep's femorofemoropopliteal for three months; then the following procedures were done. Immunohistochemistry of Ⅷ factor correlative antigen was used to identify the cells property; observation of growth condition of EC and superficial morphosis of HV leaflets was made with scanning election microscope (SEM). Results Multiple comparison showed that SI of Groups Ⅱ, Ⅲ, Ⅳ, and Ⅴ differed significantly from Group Ⅰ(P0.05), but Group Ⅲ, Ⅳ, and Ⅴ all had significant differences from GroupⅡ (P

Objective To make a comparative analysis of conventional smear and liquid-based cytology method to diagnose lung cancer in sensitivity and false-negative,and evaluate the application value of liquid-based cytology in sputum samples.Methods We collected 287 sputum samples through clinical contrast conventional smear with liquid-based cytology method.The diagnosis was made by the same pathology panel in double blind way.We compared the two methods to diagnose lung cancer in sensitivity and false-negative.Results Liquid-based cytology method detected 62 cases of lung cancer,and conventional smear detected 49 cases of lung cancer,with the sensitivity rate being 21.6% and 17.1%,respectively.Combination of the two methods made the sensitivity rate 24.0%.The false-negative rate was 38.6% for liquid-based cytology method and 48.5% for conventional smear which did not differ significantly.By combining the two methods,the false-negative rate was 24.0%.There was no significant difference between liquid-based cytology method and conventional smear,and between liquid-based cytology method and the two-method combination in sensitivity and false-negative.But there was a significant difference between the two-method combination and conventional smear.Conclusion Through sputum sample detection of lung cancer cell,liquid-based cytology method is better than conventional smear,the former having obvious superiority;combining the two methods can detect more lung cancers.Therefore,it is a superior screening test in detecting lung cancer and worthy of clinical popularization.

Objective To investigate the inhibitory effect of carbon-coated iron nanoparticles carrying cisplatin on the growth of NCI-H446 lung cancer cells and expressions of Caspase 3 and Survivin mRNA.Methods NCI-H446 lung cancer cells were treated with iron-carbon nanoparticles and/or cisplatin.The cell viability was detected by MTT method,and the mRNA expressions of Caspase 3 and Survivin were measured with RT-PCR.Results Cisplatin could inhibit the growth of NCI-H446 lung cancer cells,and the inhibitory effect was stronger when it was combined with the iron-carbon nanoparticles.The cells had apoptosis.The mRNA expression of Caspase 3 of NCI-H446 lung cancer cells was remarkably enhanced after treatment with iron-carbon nanoparticles combined with cisplatin,while the mRNA expression of Survivin was notably weakened (P＜0.05).Conclusion Carbon-coated iron nanoparticles carrying cisplatin could significantly increase the chemotherapy sensitivity of cisplatin on NCI-H446 lung cancer cells and enhance the therapeutic efficacy of chemotherapy drugs.

Objective To investigate the expression and clinical significance of long non-coding RNA SBF2-AS1 (SBF2 antisense RNA 1) in non-small cell lung cancer (NSCLC).Methods Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect SBF2-AS1 expressions in 114 cases of NSCLC tissues and the adjacent normal tissues to analyze its relationship with clinic-pathological characteristics,diagnostic value and prognosis in NSCLC.Results SBF2-AS1 expression was significantly higher in the NSCLC (4.336 ±0.032) compared with the adjacent normal tissues (1.256 ± 0.021),with a significant difference (t =3.594,P =0.005).The expression of SBF2-AS1 was related with tumor size (x2 =13.072,P =0.001),lymphatic metastasis (x2 =6.896,P =0.009),TNM stage (x2 =8.566,P =0.003),smoking history (x2 =8.769,P =0.003) and infiltration degree (x2 =17.852,P =0.001),but was not related with age (x2 =0.141,P =0.707),sex (x2 =0.036,P =0.850) and pathological type (x2 =1.267,P =0.260).The area under the receiver operating characteristic (ROC) curve was 0.853 (95 ％ CI:0.755-0.879,P =0.004).The sensitivity and specificity was 52.4％ and 87.8％,respectively.The difference betwen low SBF2-AS1 expression and high SBF2-AS1 expression groups was statistically significant in overall survival time (42.3 months vs.25.2 months,x2 =4.753,P =0.013).Conclusion The expression of SBF2-AS1 is upregulated in NSCLC and may be proved useful as a biomarker and diagnostic target for the treatment of patients with NSCLC.

Objective To explore the effect of hospital-family continuous nursing on the symptoms and life quality of COPD patients.Methods A total of 80 stable COPD patients in our hospital from July to November 2016 were selected as research objects and were divided into two groups according to the time of admission,patients in odd-numbered admission day was as observation group (hospital-family continuous nursing),and patients in even-numbered day was as control group (routine nursing),self-management,symptom control and life quality after 3 months of discharge in the two groups were compared.Results The total score of self-management after 3 months of discharge in the observation group was higher,but the CAT score was lower,and times of disease onset was less than that in the control group,the differences were statistically significant (P ＜ 0.05).The life quality scores in influence,symptoms,and activities after 3 months of discharge in the observation group was lower than that in the control group,and the difference was statistically significant (P ＜0.05).Conclusion The hospital-family continuous nursing for COPD patients can improve the self-management ability,relieve the symptoms and improve the life quality.

Objective To explore the effect of hospital-family continuous nursing on the symptoms and life quality of COPD patients.Methods A total of 80 stable COPD patients in our hospital from July to November 2016 were selected as research objects and were divided into two groups according to the time of admission,patients in odd-numbered admission day was as observation group (hospital-family continuous nursing),and patients in even-numbered day was as control group (routine nursing),self-management,symptom control and life quality after 3 months of discharge in the two groups were compared.Results The total score of self-management after 3 months of discharge in the observation group was higher,but the CAT score was lower,and times of disease onset was less than that in the control group,the differences were statistically significant (P ＜ 0.05).The life quality scores in influence,symptoms,and activities after 3 months of discharge in the observation group was lower than that in the control group,and the difference was statistically significant (P ＜0.05).Conclusion The hospital-family continuous nursing for COPD patients can improve the self-management ability,relieve the symptoms and improve the life quality.

Objective:To investigate the clinical effect on ultrasound-guided vascular access-interventional therapy of hemodialysis in day surgery mode.Methods:Hemodialysis patients with vascular access dysfunction who underwent ultrasound-guided interventional therapy in the First Affiliated Hospital of Xi'an Jiaotong University from September 1, 2018 to October 31, 2020 were retrospectively analyzed. Demographic and clinical data were collected by electronic medical record system and telephone follow-up. Kaplan-Meier method was used to analyze the patency rate of vascular access.Results:A total of 421 cases of ultrasound-guided vascular access intervention were performed in 269 patients. The technical success rates of stenosis, chronic occlusion and acute occlusion lesion were 98.8%, 90.6% and 86.4%, respectively, and 406 cases (96.4%) of 246 patients were clinically successful. The postoperative brachial artery blood flow was 821(627, 1 029) ml/min, which was significantly higher than 309(202, 453) ml/min before the operation ( Z=-13.547, P<0.001). No serious complications occurred during and after the operation. At 6, 12, 18 and 24 months after operation, the primary patency rate was 74%, 59%, 48% and 45%, respectively, the assisted primary patency rate was 94%, 91%, 88% and 82%, and the secondary patency rate was 96%, 93%, 91% and 86%. Compared with the conventional inpatient surgery mode, the total cost of the day surgery mode was significantly reduced [12 067(10 051, 13 198) yuan vs 14 986(12 411, 20 643) yuan, Z=-13.185, P<0.001], and the hospital stay was significantly shortened [5.1(3.5, 6.9) h vs 73.4(31.6, 146.6) h, Z=-13.348, P<0.001]. Conclusion:It is safe and effective to perform interventional therapy for vascular access malfunction under ultrasound in day surgery mode, which can save cost and time of hospitalization, and can be carried out in hospitals with relevant conditions.

Objective:To investigate respiratory virus infection in children with septic shock in pediatric care units (PICU) in China and its influence on clinical outcomes.Methods:The clinical data of children with septic shock in children′s PICU from January 2018 to December 2019 in 10 Chinese hospitals were retrospectively collected. They were divided into the pre-COVID-19 and post-COVID-19 groups according to the onset of disease, and the characteristics and composition of respiratory virus in the 2 groups were compared. Matching age, malignant underlying diseases, bacteria, fungi and other viruses, a new database was generated using 1∶1 propensity score matching method. The children were divided into the respiratory virus group and non-respiratory virus group according to the presence or absence of respiratory virus infection; their clinical characteristics, diagnosis, and treatment were compared by t-test, rank sum test and Chi-square test. The correlation between respiratory virus infection and the clinical outcomes was analyzed by logistic regression. Results:A total of 1 247 children with septic shock were included in the study, of them 748 were male; the age was 37 (11, 105) months. In the pre-and post-COVID-19 groups, there were 530 and 717 cases of septic shock, respectively; the positive rate of respiratory virus was 14.9% (79 cases) and 9.8% (70 cases); the seasonal distribution of septic shock was 28.9% (153/530) and 25.9% (185/717) in autumn, and 30.3% (161/530) and 28.3% (203/717) in winter, respectively, and the corresponding positive rates of respiratory viruses were 19.6% (30/153) and 15.7% (29/185) in autumn, and 21.1% (34/161) and 15.3% (31/203) in winter, respectively. The positive rates of influenza virus and adenovirus in the post-COVID-19 group were lower than those in the pre-COVID-19 group (2.1% (15/717) vs. 7.5% (40/530), and 0.7% (5/717) vs. 3.2% (17/530), χ2=21.51 and 11.08, respectively; all P<0.05). Rhinovirus virus were higher than those in the pre-Covid-19 group (1.7% (12/717) vs. 0.2% (1/530), χ2=6.51, P=0.011). After propensity score matching, there were 147 cases in both the respiratory virus group and the non-respiratory virus group. Rate of respiratory failure, acute respiratory distress, rate of disseminated coagulation dysfunction, and immunoglobulin usage of the respiratory virus group were higher than those of non-respiratory virus group (77.6% (114/147) vs. 59.2% (87/147), 17.7% (26/147) vs. 4.1% (6/147), 15.6% (25/147) vs. 4.1% (7/147), and 35.4% (52/147) vs. 21.4% (32/147); χ2=11.07, 14.02, 11.06 and 6.67, all P<0.05); and PICU hospitalization of the former was longer than that of the later (7 (3, 16) vs. 3 (1, 7)d, Z=5.01, P<0.001). Univariate logistic regression analysis showed that the presence of respiratory viral infection was associated with respiratory failure, disseminated coagulation dysfunction, the use of mechanical ventilation, and the use of immunoglobulin and anti-respiratory viral drugs ( OR=2.42, 0.22, 0.25, 0.56 and 1.12, all P<0.05). Conclusions:The composition of respiratory virus infection in children with septic shock is different between pre and post-COVID-19. Respiratory viral infection is associated with organ dysfunction in children with septic shock. Decreasing respiratory viral infection through respiratory protection may improve the clinical outcome of these children.

Objective:To investigate the clinical features, pathogen composition, and prognosis of septic shock in pediatric intensive care units (PICU) in China.Methods:A multicenter retrospective cohort study. A retrospective analysis was conducted on the clinical data of children with septic shock from 10 hospitals in China between January 2018 and December 2021. The clinical features, pathogen composition, and outcomes were collected. Patients were categorized into malignant tumor and non-malignant tumor groups, as well as survival and mortality groups. T test, Mann Whitney U test or Chi square test were used respectively for comparing clinical characteristics and prognosis between 2 groups. Multiple Logistic regression was used to identify risk factors for mortality. Results:A total of 1 247 children with septic shock were included, with 748 males (59.9%) and the age of 3.1 (0.9, 8.8) years. The in-patient mortality rate was 23.2% (289 cases). The overall pathogen positive rate was 68.2% (851 cases), with 1 229 pathogens identified. Bacterial accounted for 61.4% (754 strains) and virus for 24.8% (305 strains). Among all bacterium, Gram negative bacteria constituted 64.2% (484 strains), with Pseudomonas aeruginosa and Enterobacter being the most common; Gram positive bacteria comprised 35.8% (270 strains), primarily Streptococcus and Staphylococcus species. Influenza virus (86 strains (28.2%)), Epstein-Barr virus (53 strains (17.4%)), and respiratory syncytial virus (46 strains (17.1%)) were the top three viruses. Children with malignant tumors were older and had higher pediatric risk of mortality (PRISM) Ⅲ score, paediatric sequential organ failure assessment (pSOFA) score (7.9 (4.3, 11.8) vs. 2.3 (0.8, 7.5) years old, 22 (16, 26) vs. 16 (10, 24) points, 10 (5, 14) vs. 8 (4, 12) points, Z=11.32, 0.87, 4.00, all P<0.05), and higher pathogen positive rate, and in-hospital mortality (77.7% (240/309) vs. 65.1% (611/938), 29.7% (92/309) vs. 21.0% (197/938), χ2=16.84, 10.04, both P<0.05) compared to the non-tumor group. In the death group, the score of PRISM Ⅲ, pSOFA (16 (22, 29) vs. 14 (10, 20) points, 8 (12, 15) vs. 6 (3, 9) points, Z=4.92, 11.88, both P<0.05) were all higher, and presence of neoplastic disease, positive rate of pathogen and proportion of invasive mechanical ventilation in death group were also all higher than those in survival group (29.7% (87/289) vs. 23.2% (222/958), 77.8% (225/289) vs. 65.4% (626/958), 73.7% (213/289) vs. 50.6% (485/958), χ2=5.72, 16.03, 49.98, all P<0.05). Multiple Logistic regression showed that PRISM Ⅲ, pSOFA, and malignant tumor were the independent risk factors for mortality ( OR=1.04, 1.09, 0.67, 95% CI 1.01-1.05, 1.04-1.12, 0.47-0.94, all P<0.05). Conclusions:Bacterial infection are predominant in pediatric septic shock, but viral infection are also significant. Children with malignancies are more severe and resource consumptive. The overall mortality rate for pediatric septic shock remains high, and mortality are associated with malignant tumor, PRISM Ⅲ and pSOFA scores.