Objective To investigate the correlated issues in the stenting treatment of major airway constriction. Methods Nineteen cases of major airway stenting procedure were studied retrospectively. The clinical choice of stents of different advantages or deficiencies were discussed. The importances of intravenous anesthesia supporting, life parameters monitoring during the procedures and the prevention of complications were analysed. Results Under intravenous and local anesthesia, 19 Wallstents had been successively placed and relieved 19 cases of major airway constrictions due to malignant or benign diseases (15 of tumors, 3 of tuberculosis, 1 of tracheomalacia). Intravenous anesthesia and life parameters monitoring had made the procedures more safe and precise. Conclusions Major airway stenting is an reliable method for relieving trachiobronchial stenosis; and intravenous anesthesia supporting and life parameters monitoring guarantee the satisfactions of procedures.

OBJECTIVE:To investigate the relationship between the perioperative application of antibiotics in caesarean opera-tion and neonatal hyperbilirubinemia,and to provide reference for reducing the incidence of neonatal hyperbilirubinemia. METH-ODS:264 cases of cesarean section women were randomly divided into control group and observation group,with 132 cases in each group. Control group was given antibiotics after operation,and observation group was given prophylactic application of antibi-otics before operation,and equivalent dose of intravenous injection after operation. Postoperative maximum and average tempera-ture,white blood cell(WBC),neutrophils(NEUT),complications and economic index were compared between 2 groups after op-eration. RESULTS:There was no statistically significant difference in postoperative maximum and average temperature,WBC count,NEUT proportion,wound infection and fever rate between 2 groups (P>0.05). The costs of medication,drug dosage per capita,hospital stay and the incidence of neonatal hyperbilirubinemia in observation group were significantly lower than in control group,with statistical significance (P<0.05). CONCLUSIONS:The perioperative standard use of antibiotics in caesarean opera-tion could effectively reduce the incidence of postoperative neonatal hyperbilirubinemia and hospitalization cost,and cut down treat-ment time. It also has a high economic value and worthy of promotion.

Objective To investigate the effects and mechanism of Zhixie recipe on small intestinal motility of rats and absorption ability of rabbits.MethodsThree groups of rats,one was fed with distilled water,the another two were fed with Zhixie recipe liquid of 1 g/ml and 2 g/ml.Then all three groups were fed with ink,and the moving distance of the ink in small intestines was measured to check the small intestinal motility; Three mixtures of Zhixie recipe liquid and desktop solution with 0％,5％ and 10％ Zhixie recipe liquid were injected into rabbits,the Chloride ion concentration in small intestines was measured to check the absorption ability.ResultsThe average moving distance percentage of ink in the small intestines of the three groups of rats was 52.55％ ( SD =8.24),54.35％ (SD =12.53 ) and 59.45％ ( SD=14.17) of the total intestine length respectively.It showed that Zhixie recipe had no influence on small intestinal motility (P＞0.05) ; Small intestines absorption experiments revealed that the small intestinal absorption ability was quite different between the rabbits with desktop solution and the rabbits with mixtures of Zhixie recipe liquid and Desktop solution(5％ and 10％),with x2=6.92 and 46.18,P＜0.01.The mixtures with 5％ and 10％ Zhixie recipe liquid improved the small intestinal absorption ability,and the effect of the mixture with 10％ was even stronger.Conclusion Zhixie recipe showed no influence on small intestinal motility,but it improved the small intestinal absorption ability.

Objective To explore the effect of Wuji Solution (He Ji Ju Fang) on excreting IL-8 of SGC-7901 cells infected with Helicobacter pylori (H?pylori) in vitro. Methods H?pylori was inoculated to SGC-7901 cells. ELISA was applied to examine the production of interleukin-8 (IL-8) of cells infected with H?pylori. Results The production of IL-8 of infected cells was lower than that of control group (P

Objective:To investigate the efficacy of montelukast in the treatment of children with asthma and its influence on the serum levels of metalloprotease-9 (MMP-9) and metal protease inhibitor-1 (TIMP-1).Methods:Totally 90 cases of asthma children were selected and divided into the control group and the observation group with 45 ones in each .The control group was treated with the conventional symptomatic treatment , and the observation group was treated with montelukast chewable tablets additionally , and the treatment course was 8w.The effectiveness adverse reactions were compared between the groups , and the lung function indices and the changes of MMP-9 and TIMP-1 levels of the two groups were compared as well .Results: The total effective rate of the observation group (93.33%) was significantly better than that of the control group (P<0.05).After the treatment, the levels of FEV1 and PEF were significantly higher than those before the treatment in the two groups , the levels of MMP-9 and TIMP-1 were notably lower than those before the treatment in both groups , and the changes in the observation group were more significant than those in the control group (P<0.05).There were no adverse drug reactions in both groups .Conclusion:Montelukast combined with the conventional therapy can down-regulate the serum levels of MMP-9 and TIMP-1 and inhibit airway remodeling resulting in improved curative effect with high safety.

Objective To assess the association between the depth of trophoblastic penetration into the tubal wall with serum concentrations of vascular endothelial growth factor (VEGF) and β-human chorionic gonadotropin (β-HCG). Meth-ods Eighty patients with a diagnosis of tubal pregnancy in the ampullary region underwent radical surgical treatment (sal-pingectomy), were included in this study. The serum levels of VEGF andβ-HCG were detected on the day of surgery. The se-rum level of VEGF was measured by ELISA. The serum level ofβ-HCG was quantified with a two-site immunofluorimetric assay based on the direct sandwichtechnique. Histological material was stained with Masson's trichrome to identify muscular fibers. Immunohistochemical staining was used for human placental lactogen (hPL) to identify intermediate trophoblast and determine the depth of trophoblastic invasion into the tubal wall. The ampullary pregnancies were classified histologically ac-cording to the depth of trophoblastic infiltration into the tubal wall. Results The mean serum values of VEGF andβ-HCG were significantly lower in patients with stage I tubal infiltration than those of stageⅡ, and which were significantly lower in patients with stageⅡthan those in stageⅢ(P<0.05). The threshold serum value of VEGF was 308.6 ng/L, the sensitivity was 100.0%and the specificity was 92.6%for stageⅠand stageⅡ. The threshold serum value of VEGF was 431.9 ng/L, the sensitivity was 79.3%and specificity was 79.2%for stageⅡandⅢ. The threshold serum value ofβ-hCG was 2 509.6 IU/L, the sensitivity was 91.7%and specificity was 81.5%for stageⅠand stageⅡ, and levels of 13 142.6 IU/L, 72.4%and 95.8%for stageⅡand stageⅢ. Conclusion The depth of trophoblastic penetration into the tubal wall is associated with maternal serum concentrations of VEGF andβ-HCG, which can be used as the evaluation index for histological staging of trophoblas-tic tissue infiltration.

Objective To investigate the protective effect and possible mechanism of Decorin on pulmonary fibrosis.Methods A total of 90 healthy Wistar rats were randomly divided into three groups:group A served as control group,group B was injected BLMA5 through trachea,group C was injected BLMA5 and received treatment of Decorin.Ten rats in each group were killed 7、14、28 days after intratrachael instillation.TGF-?_1 expression was measured by immunohistochemical technique.The degree of alveolitis and fibrosis were measured by HE staining.The contents of hydroxideproline were measured in the homodenate of the rat lung.Results The expressions of TGF-?_1 were increased gradually in group B;in group C the expressions were decreased significantly than group B(P

Objective To investigate the oxidative mechanism of homocysteine ( Hey) -induced apoptosis in endothelial progenitor cells( EPCs). Methods Total mononuclear cells were isolated from mouse bone marrow by Ficoll density gradient centrifugation and were cultured in vitro for 7 d. Adherent cells were harvested and identified by fluorescence microscopy. EPCs were cultured with Hey (0, 50, 100 and 500 μmol/L) for 12, 24 and 48 h, or pretreated with NAC (1 mmol/L), DPI( 10 μmol/L) or SB203580 (10 μmol/L) for 30 min, then cultured with 500 μmol/L Hey for 24 h. Apoptosis was detected by Annexin-V/PI flow cytometry, levels of reactive oxygen species (ROS) in cells were measured using H2DCF-DA as a fluorescence probe, NADPH oxidases were evaluated with lucigenin-enhanced chemilumine9cence, and NO in the supernatant was determined by nitrate reductase assay. Results Hey induced EPCs apoptosis, ROS accumulation, NADPH oxidase activation and decrease of NO in a time-dose dependent manner( P <0.05 or P < 0.01). Pretreatment with NAC, DPI and SB203580 could inhibit these effects (P < 0.05 or P < 0.01). Conclusion Hey could activate NADPH oxidase, induce ROS increase and NO decrease, and activate p38MAPK to enhance EPCs apoptosis.

OBJECTIVE:To observe therapeutic efficacy and safety of low-molecular-weight-heparin-sodium combined with dy-drogesterone in the treatment of threatened abortion. METHODS:Medical information of 72 patients with threatened abortion were analyzed retrospectively and divided into control group(36 cases)and observation group(36 cases). Control group was given Dy-drogesterone tablet with initial dose of 40 mg,one day later every 12 h 10 mg/time. Observation group was additionally given ab-dominal subcutaneous injection of Low-molecular-weight-heparin-sodium injection 5000 U,once a day,on the basis of control group. Both groups were treated for a week. Clinical efficacies of 2 groups were observed,and lumbar acid,vaginal bleeding,ab-dominal pain time,total treatment time,neonatal birth weight were also observed. The levels of hs-CRP,fibrinogen,D-dimer and platelet,the occurrence of ADR were observed before and after treatment. RESULTS:The total response rate of observation group was significantly higher than that of control group(91.7%vs. 80.6%),the backache,vaginal bleeding,abdominal pain time and to-tal treatment time of observation group were significantly shorter than those of control group,the neonatal birth weight and gesta-tional age of observation group were significantly more than those of control group,with statistical significance(P<0.05). Before treatment,there was no statistical significance in the levels of hs-CRP,fibrinogen,D-dimer and platelet between 2 group(P>0.05);after treatment,the levels of hs-CRP,fibrinogen,D-dimer and platelet in 2 groups were significantly lower than before,and the observation group was significantly lower than the control group,with statistical significance (P<0.05). There was no statistical significance in the incidence of ADR between 2 groups (P>0.05). CONCLUSIONS:Based on routine treatment,low-molecu-lar-weight-heparin-sodium combined with dydrogesterone shows good therapeutic efficacy and safety for threatened abortion.

Objective To observe the clinical curative effect of zhidanhuayu decoction on patients with carotid atherosclerotic plaque.Methods 80 patients with carotid atherosclerosis were randomly divided into two groups, each group of 40 cases, the control group was treated with rosuvastatin calcium 20 mg, once every night.The treatment group in the control group on the basis of a scheme based on the use of night zhidanhuayu decoction water 500 mL temperature and heat to 200 mL, then add water and cook until 200 mL to 100 mL, on the afternoon of two phase decoction against half amount of clothes, a daily agent;the two groups were 3 months for 1 courses.Before and after treatment, the mean medial thickness(IMT), plaque score, blood lipid and adverse reactions were observed.Results There was significant difference between the two groups before and after treatment, the difference was significant between the medial and middle thickness(IMT), plaque score and blood lipid(P< 0.05).After treatment, compared with the control group, the treatment group and the control group, IMT, plaque score, blood lipids were statistically significant(P< 0.05).Conclusion Zhidanhuayu decoction can adjust the blood lipids, but also has the effect of anti atherosclerosis.