The expansion of high-containment bio-defense laboratory in USA is introduced in such aspects as the increase of laboratory magnitude,amplification of laboratory area and the funding growth of the United States National Institutes of Health(NIH).In addition,a series of problems caused by the expansion are analyzed in the perspective of the biosafety training,information exchange of laboratory and public participation.

Objective PRDX-2 of the basic level in patients with PCOS,and its correlation with cardiovascular risk factors.Methods Collected 78 cases of PCOS patients for POCS group,at the same time,choose healthy check-up 40 cases for control group.Determination of plasma Peroxiredoxin-2,blood sugar,glycosylated hemoglobin,cholesterol,triglycerides,and other biochemical indicators.Results Peroxiredoxin-2 level in PCOS group are higher than the control group(F=26.03;P<0.05).Peroxiredoxin-2 and cardiovascular risk factors exist correlation,including systolic blood pressure (r=0.213),diastolic blood pressure (r=0.380),fasting blood glucose (r=0.308),glycosylated hemoglobin (r=0.303),cholesterol (r=0.190),triglyceride (r=0.276),LDL-C (r=0.484) were positively correlated,and CRP (r=0.227),HDL-C negative correlation (r=0.355).Conclusion Determination of Peroxiredoxin-2 level can be used as evaluation of patients with PCOS risk factors for cardiovascular disease.

Objective To observe the clinical efficacy of acupoint application and moving cupping plus photo ionization treatment apparatus in treating hyperplasia of mammary glands. Method Sixty patients with hyperplasia of mammary glands were randomized into a treatment group and a control group, 30 in each group. The treatment group was intervened by acupoint application and moving cupping plus photo ionization treatment apparatus, while the control group was by oral administration of Tamoxifen and Ru Pi San Jie tablets. The breast pain and lump score was observed before and after 2-month treatment, and the clinical efficacy was compared. Result The breast pain and lump scores were significantly changed in both groups after intervention (P<0.05). After intervention, there was no significant difference in comparing the breast pain and lump score between the two groups (P>0.05). The total effective rate was 96.7% in the treatment group versus 86.7% in the control group, and the difference was statistically significant (P<0.05). Conclusion Acupoint application plus moving cupping plus photo ionization treatment is an effective method in treating hyperplasia of mammary glands, and it can ease or reduce breast pain, and improve or reduce the lump.

Objective To explore the influence of aerobic exercise on plasma lipoprotein in overweight and obese PCOS women.Methods 12 obese and overweight patients with PCOS were selected.Before and after 12 weeks of aerobic exercise,the height,weight,BMI,WHR,body fat percentage and fasting blood samples of high-density lipoprotein(HDL),low density lipoprotein(VLDL),triglyceride and cholesterol levels were measured and detected.Results After 12 weeks of aerobic exercise,the BMI[(25.43±2.17)kg/cm2 vs.(22.27±1.57)kg/cm2,t=8.52],WHR[(0.864±0.021) vs.(0.831±0.019),t=2.99],fat rate[(33.76±5.36)% vs.(30.53±5.66)%,t=5.580],and triglyceride[(1.90±0.58)mmol/L vs.(0.95±0.51)mmol/L,t=7.400]were obviously decreased,the high-density lipoprotein cholesterol[(2.07±0.39)mmol/L vs.(3.31±0.48)mmol/L,t=3.352]was obviously increased,the differences were statistically significant (all P<0.05).Conclusion Obese and overweight PCOS women can lose weight through aerobic exercise,so as to adjust lipoprotein spectrum and improve health.

0.05). Conclusion The extract technology is basically reasonable,and need further improvement.

Objective To study the role of plasma levels of gastrointestinal hormones and gallbladder kinetics in the pathogenesis of functional dyspepsia. Methods Thirty two patients with functional dyspepsia (FD) and 20 normal subjects as controls were studied. Gallbladder ejection fractions (GBEF) were determined with 99m Tc radionucleography, and plasma levels of motilin (MTL)、cholecystokinin (CCK)、 vasoactive intestinal peptide (VIP) and somatostatin (SS) were measured with radioimmunoassay. Results Both preprandial and postprandial GBEF and MTL levels were markedly lower in FD group than those in control group ( P 0.05), while the levels of postprandial CCK were significantly decreased in FD group ( P 0.05). Conclusion (1) Patients with FD might have a decreased level of preprandial and postprandial gallbladder emptying. The decrease of plasma levels of MTL、CCK and the elevation of VIP might be the cause of gallbladder slow emptying in FD. (2) Radionucleography is non invasive and safe. It can be used in the quantitative analysis and dynamic study of gallbladder and Oddi sphincter functions, and provide accurate, physiological results.

Objective: To evaluate the preemptive analgesia of intravenous tramadol preoperatively in neck-surgery. Methods: Eighty patients, ASAⅠ-Ⅱ,were scheduled for neck-surgery under local anesthesia. Patients were randomly divided into 2 groups (40 cases/group).In group P intravenous tramadol was administered as a bolus dose of 3mg/kg 15min prior to skin incision. Group C received saline as a placebo. The patients-controlled analgesia(PCA) devices were used in all patients after operation. We observed BP, HR,VAS score, the time of first PCA activation, the valid number of press PCA and the total consumption of analgesic after operation. Results:The postoperative VAS was markedly lower in group P compared with the group C in 0-24h( P

Objective To explore the effects of air pollution on daily outpatient vivits of hospital. Methods Correlation analysis was carried out based on data of daily outpatient visits of Taiyuan Central Hospital(TCH) and monitoring data on the concentration of TSP and SO2 in air and temeprature and humidity area of Xinghualing district where TCH was located by the time series analysis. Results The concentiations of TSP and SO2 in air exceeded the corresponding standards recommended by WHO. SO2 was the statistically significant forecasting factor for the daily outpatient visits of the department of respiratory disease, emergency and piediatrics. TSP was the statistically singnificant forecasting factor for the daily outpatient visits of department of cardiovascular disease and other departments. Conclusion Severe air pollution by TSP and SO2 effected the dialy outpatient visits in a certain degree.

Objective To study on gastric varices bleeding treated by tissue adhesive, D-TH glue injection. Methods Fifty-nine consecutive cirrhotic patients with gastric varices were undergone endoscopic D-TH glue injection with sandwich approach. Among these 59 patients, bleeding was present in 46 patients (acute bleeding in 37, recent bleeding in 9) , no previous of gastric variceal bleeding in 13. Follow-up study on therapeutic results and complications were conducted. Results Ninety-six injections were performed in all patients with a mean of 1. 63 injections (1-2ml) per patient. Recurrent bleeding never occurred in 37 patients with acute gastric varices bleeding 72 hours after injection, and the rale of emergency endoscopic he mostasis was 100 per cent. Thirty (65. 2% ) of 46 patients with bleeding gastric varices were followed for 12 36 months, 24. 9 months on average. Recurrence of bleeding occurred in 2(6. 7% ) , 5 ( 16. 7% ) and 8 (26. 7% ) cases at 3 , 12, and 36 months respectively. The rate of survival was 86. 5 per cent. Besides fever occurred postoperatively in 2 cases, other severe complications never happened. Conclusion Endoscopic D-TH glue injection is a safe and effective method for treating gastric varices bleeding.

Objective To detect the hypermethylation of HOXB6 gene in gastric cancer tissue and investigate the relationship between the gene expression and the clinics.Methods seven types of gastric cancer cell line and 73 cases of gastric cancer biopsy tissues were measured by combined bisulfite restriction analysis method.The gene expression and the re-expression after demethylation were detected by RT-PCR.Results Of 7 cell lines examined, 4 showed hypermethylation.The methylating status of HOXB6 was found to be correlated with loss of expression,which was restored by 5-aza-2＇-deoxycytidine treatment.We also find hypermethylation in 20/73(27.40%)case of cancer tissues but no one in non cancer tissue.Conclusion There are close relationship between hypermethylation and gene silencing in the HOXB6 gene of gastric cancer,There are also a gene re-expression after its demethylation.Hypermethylation of HOXB6 gene can be a tumor marker for gastric cancer diagnosis,and the agent of demethylation maybe a new antineoplastic drug.