One of the challenge of pediatricians at follow -up for high risk babies is how to identify early in-fants who is at risk of neurodevelopment disability and timely intervention,another is to find infants who will not have a neurodevelopment sequel in order to avoid unnecessary excessive intervention as soon as possible.Accurate diagnosis can be achieved through comprehensive analysis by combined with the basic knowledge,neuroimaging and follow -up of neonatal brain injury.

The morbidity of transient hypothyroxinemia of prematurity (THOP) was high. There is controversial about thyroid hormone supplementation in THOP. Clinical studies suggest that thyroid hormone supplementation can reduce the incidence of patent ductus arteriosus,but no effect on the mortality and the incidence of respiratory disease.Thyroid hormone supplementation can improve the neurodevelopmental outcomes in infants with gestational age less than 28 weeks with THOP, but no effect on infants with gestational age more than 28 weeks. Future research should focus on the normal range of thyroid hormone according to gestational age in preterm infants,and randomized clinical trial welldesigned stratified according to gestational age to study the long-term neurodevelopmental outcome of thyroid hormone replacement therapy in infants with THOP

Objective To study the safety and efficacy of selective head cooling (SHC) with mild systemic hypotherrnia in neonates with HIE. Methods Fifty-four term infants with severe neonatal HIE were randomly assigned to the head cooling group (n=27) and control group (n=27). Forty-one infants in 96 h after admission were eligible for the study(SHC group n=21, control group n=20). In SHC group, the naso-pharyngeal temperature was maintained at (34.0±0.2) ℃ and rectal temperature maintained at 34～35 ℃ for 72 h, then rewarmed spontaneously. In control group, normal rectal temperature was maintained. During the period of the study, the infants of two groups were monitored on nasopharyngeal temperature, heart rate, respiratory rate,transcutaneous arterial oxygen saturation and blood pressure. Primary adverse effects inclu-ding severe arrhythmia, venous thrombosis or hemorrhage and severe hypotension were observed. The efficacy indicators including rate of death and severe disability, exercise and cognition development index were as-sessed. Results Severe arrhythmia, hypotension and renal failure were not found in both groups. Follow-up was conducted until postnatal 18 months and was not available in 6 babies (3 in SHC group and 3 in control group respectively). Death and severe disability occurred in 4 of 18 infants (22.2%)in SHC group and in 9 of 17 infants(52.9% ) in the control group respectively (P<0.05). Conclusion SHC for 72 h with mild systemic hypothermia in neonates with HIE is safe and effective. The therapy could reduce the risk of disabili-ty and handicap significantly.

OBJECTIVEThis study aims to evaluate the shaping capability of Reciproc, WaveOne, Mtwo, and ProTaper instruments in simulated root canals.

METHODSA total of 40 simulated resin blocks were divided randomly into four groups. Each group was prepared with Reciproc (Group 1), WaveOne (Group 2), Mtwo (Group 3), and ProTaper (Group 4). The preparation time and reduction in working length after preparation were measured. Pre- and post-operative images were obtained with a scanner and superimposed through Photoshop. The changes in canal curvature and material removal from the inner and outer canal walls at 10 points beginning at 1 mm from the end point of the canal were measured with Image J. Centering capability was determined accordingly. Data were analyzed through one-way ANOVA, SNK, and Kruskal-Wallis at a significance level of P < 0.05.

RESULTSThe preparation time of Group 2 was (53.7 ± 6.7) s, whereas those of Groups 1, 3, and 4 were (86.9 ± 8.1) s, (112.2 ± 8.2) s, and (177.9 ± 11.2) s, respectively; the difference was found to be significant (P < 0.05). The reductions in working length among the four groups after preparation were not significantly different (P > 0.05). The canal curvature for Groups 1 to 4 were 2.671° ± 0.637°, 2.667° ± 0.450°, 3.664° ± 0.870°, and 3.797° ± 0.601°, respectively. The changes for Groups 1 and 2 were significantly smaller than those for Groups 3 and 4. At the 3 mm point, the transportation of Group 1 was (-0.016 ± 0.094) mm, which was significantly less than that of the other instruments (P<0.05). At the 4 mm and 5 mm points, the trans- portation values of Group 2 were (-0.080 ± 0.104) mm and (-0.312 ± 0.088) mm, which were significantly less than that of Group 1 [(-0.243 ± 0.099) mm, (-0.404 ± 0.064) mm, P < 0.05].

CONCLUSIONReciproc and WaveOne can complete preparation faster and can maintain the original canal curvature better than Mtwo and ProTaper. Reciproc exhibits superior centering capability in the apical part of the canal, whereas WaveOne exhibits superior centering capability in the middle part of the canal.

Objective To study the safety of hypothermia in neonates with hypoxic-ischemic brain damage after asphyxia. Methods Twenty four full term newborns with Apgar score 0.05). Conclusions The results suggest that the selective head cooling in full term newborn has no significant adverse effect on cardiac function, kidney function and coagulation function.

[Objective]To observe the intervention of Electroacupuncture at Point Jianyu(LI15) on Rabbit Model of Scapulohumeral Periarthritis for receiving possible mechanism.[Methods ]Thirty healthy rabbits(male or female) were randomly divided into three groups:scapulohumeral periarthritis model group,EA Jianyu group and Ibuprofen control group.Each group respectively were detected the concentrations of serum PGE2 before model and treatment and after treatment,and then killed them to achieve tendons of shoulder which were detected the concentrations of Hydroxyproline.And achieve tissues around shoulder which were studied by HE to investigate the changes of the histology.[Results]The concentrations of serum PGE2 had no notable difference between EA Jianyu and model group,Ibuprofen control group and model group after treatment.But it was lowered after treatment than before treatment by EA Jianyu.There were no significantly difference among the three groups about concentrations of Hydroxyproline in tendons,but Ibuprofen control group was higher than EA Jianyu group.Tissues of the shoulder were observed that EA Jianyu restrained reaction of inflammation and degenerescence of tissues,and promoted it better.[Conclusions] EA Jianyu has anti-inflammatory effect and cuts back on the degree of degenerescence of tissues on scapulohumeral periarthritis model in rabbit.

Objective To investigate the effect of exogenous GAs on learning and memory capability of hypoxic-ischemic(HI) brain damage rats. Methods After ligation of the right carotid artery of 7-day old male SD rats and waiting for 4 hours of recovery, the rats were exposed to 8% oxygen-92% nitrogen gas mixture for 2 hours. The HI animals were randomly divided into 3 groups. GAs-treated group 1: animals were injected i.p. GAs immediately after HI every 12 hours for 4 doses with 50 mg/kg, followed by a 2 weeks consecutive daily injections at dose of 30 mg/kg/day. GAs treated group 2: rats received GAs 50 mg/kg immediately after HI every 12 hours for 4 doses, followed by injection of saline in the same volums for 2 weeks. Control group: rats received saline in the equivalent volums and duration. The behavioral test starded when the animals were 24 days old. Discriminative learning ability and memory retention were tested in the tri-equal-arms maze . The locomotor activity and alternative behavior was observed. The degree of neuronal damage were assessed after behavioral test. Results (1)The number of trails to reach the learning criteria were (39?17), (32?14)and (20?13) for control group, GAs-treated group 2 and GAs-treated group 1, respectively. The percentages of memory retention for control group, GAs-treated group 2 and GAs-treated group 1 were (62?10)%, (64?11)% and (70?9)%, respectively. Two weeks consecutive daily injection of GAs had facilitatory effects on learning ability and memory retention of HI rats(P0.05). Conclusion Gangliosides can enhance the learning ability and memory retention in neonate rats with HI.

In the present paper,the authors review progresses of researches on the protective effect of acupuncture in resisting acute myocardial ischemia-reperfusion injury in patients and animals from 1) resisting injury of oxygen-derived free radicals,2) reducing intracellular Ca2+ overload,3) relieving dysfunction of vascular endothelial cells of the myocardium,4) regulating immune functional activity and reducing inflammatory injury,5) reducing cardiocyte apoptosis,6) upregulating the expression of heat shock protein(HSP 70) in the myocardium,and 7) regulating activities of multiple cardiovascular protection factors and intracellular signaling pathways.The abovementioned results are mainly obtained from experimental animal studies.For this reason,the authors hold that the coming researches on acupuncture therapy for cardiovascular disorders should pay more attention to its clinical application,for example,controlled blood-pressure decline induced lower perfusion of the coronary artery,cardiac surgery(percutaneous tranluminal coronary angioplasty,intracardiac operation underdirect vision) etc.

[Objective]The intervention of electroacupuncture (EA) on chronic inflammation and cyclooxygenase-1/-2 mRNA expressions in rats with II collagen-induced arthritis was observed in vivo, so as to discuss the effect of EA on chronic inflammation and its pivotal mechanism. [Method]Wistar rats immunized intradermally with bovine type II collagen, were served as the animal models of chronic inflammation. EA stimulation, begun on day 11 after the injection, was applied at the acupoint equivalent to ST36 for continuous 10 times. Indomethacin and refecoxib were used respectively as the control treatments of NSAID inhibitor and COX-2 selective inhibitor. The arthritic index, swelling volume of paw and concentrations of prostaglandin E_2 (PGE_2) of local tissue were investigated. Furthermore, we investigated the impact of EA on the levels of COX-1 mRNA and COX-2 mRNA in synovial tissues and gastric mucosa of inflammatory rats by using reverse transcriptase-polymerase chain reaction (RT-PCR). [Results]All collagen-injected rats developed chronic inflammation of paws. EA remarkably reduced the paw swellings of rats on day 16 and day 21 and attenuated the severity of arthritis from the day 14 to day 21. The levels of PGE_2 in the inflammatory tissues of swollen paws were also lowered by EA. EA stimulation obviously down-regulated the expression of COX-2 mRNA in synovial tissue, but did not influence the level of COX-1 mRNA expressed in gastric mucosa. Indomethacin and refecoxib also strongly down-regulated COX-2 expression in synovial tissue. However, either indomethacin or refecoxib strongly affected the COX-1 mRNA expressions in gastric mucosa. [Conclusion] EA has a pronounced anti-inflammatory effect on collagen-induced arthritis, and the pivotal mechanism and also the advantage of its therapeutic result may be due to selective suppression of COX-2 gene activation, without destroying COX-1 activity in stomach.

Objective To study the changes of cerebral blood flow and cerebral oxygenation during selective head cooling (SHC) in neonates with hypoxic-ischemic brain damage (HIBD). Methods Thirty-eight neonates with moderate and severe HIBD were enrolled in two groups: SHC group (n=18) and normal temperature group (n=20). The gestational age of all subjects were more than 38 weeks and Apgar score in 5 minutes after birth was less than 5 scores. Also, nerve system symptoms and electroencephalogram abnormal was found in 6 hours after birth. Neonates in SHC group had nasopharyngeal temperature maintained 34℃ and in normal trmerature guoup had rectum temperature maintained about 36℃. Seven healthy neonates were as control group. Three groups were observed 84 h. Cerebral blood flow velocity (CBFV) of middle cerebral artery (MCA) was measured with transcranial Doppler (TCD) at 6, 12, 24, 48, 72 and 84 hours after birth. Cerebral cytochrome aa3 (Cytaa3 ) was monitored continuously throughout the study using near infrared spectroscopy (NIRS). Results At 6th hours after birth, Vs [(25. 05?7. 69)cm/s]、Vd [(7. 22?3. 01) cm/s] and Vm [(15. 61?5. 97) cm/s] of MCA and △Cytaa3 in SHC group were lower, and RI (0. 81?0. 15) was higher than control group (P