The paper focuses on the status of hospital libraries and the factors that effect the development, proposes to establish subject librarian mechanism, uses concept and mechanism of modern scientific management, describes the employment, positioning, respon-sibility, examination, social status and payment, in order to push forward the development of hospital libraries.

Objective To investigate the relationship between coronary myocardial bridge and the symptoms of the patients and its related factors, and to provide evidence for rational treatment. Methods Sixty-one patients di-agnosed as myocardial bridge by coronary angiography and the examination of nuclide myocardial perfusion imaging were recruited and their clinical data was analyzed retrospectively. The patients were divided into ischemic group and non-ischemic group according to the nuclide imaging, and were followed up on the compliance of β-blocker taking and the symptoms. Results Among the 61 patients ,7 patients were ischemic,and the others were non-ischemic. There was no significant difference on symptoms between the two groups (P>0.05). The prevalence of Grade Ⅲ narrowing was higher in ischemic group (6/7) than that in the non-ischemic group (18/54) (χ~2=5. 009, P = 0.024) ,and the ischemic patients with Grade Ⅲ narrowing were older than those in the non-ischemic patients. Two patients in the ischemic group did not insist on taking β-blocker and their symptoms did not change,40 patients in the non-ischemia group did not insist on taking β-blocker,of which 33 patient's symptoms were improved. The symp-toms of all patients with good compliance of β-blocker taking were improved. Conclusions The symptoms of some patients are unrelated with myocardial bridge and treatment is not recommended.

Objective To detect the hypermethylation of HOXB6 gene in gastric cancer tissue and investigate the relationship between the gene expression and the clinics.Methods seven types of gastric cancer cell line and 73 cases of gastric cancer biopsy tissues were measured by combined bisulfite restriction analysis method.The gene expression and the re-expression after demethylation were detected by RT-PCR.Results Of 7 cell lines examined, 4 showed hypermethylation.The methylating status of HOXB6 was found to be correlated with loss of expression,which was restored by 5-aza-2＇-deoxycytidine treatment.We also find hypermethylation in 20/73(27.40%)case of cancer tissues but no one in non cancer tissue.Conclusion There are close relationship between hypermethylation and gene silencing in the HOXB6 gene of gastric cancer,There are also a gene re-expression after its demethylation.Hypermethylation of HOXB6 gene can be a tumor marker for gastric cancer diagnosis,and the agent of demethylation maybe a new antineoplastic drug.

0.05),while there was significant difference among other groups(P

Objective To evaluate multi- slice spiral CT angiography (MSCTA) in diagnosing bronchial-pulmonary shunts (BPS). Methods The materials of MSCTA images in 31 patients with DSA-confirmed BPS were retrospectively analyzed. Taking DSA imaging as the reference standard, the consistency in diagnosing BPS at different levels between MSCTA and DSA was compared. Results On MSCTA using aorta-density trigger scanning technique BPS was manifested as wrong-time enhancement of pulmonary artery branches. Wilcoxon test indicated that MSCTA had a high concordance with DSA in detecting BPS occurring at sub-segmental (or above) pulmonary artery branches (Z = -1.854, P > 0.05), but MSCTA detection of BPS occurring at peripheral pulmonary arteries was obviously poorer than DSA (Z = -3.923, P < 0.05). MSCTA confirmed the diagnosis of BPS in 11 cases with a sensitive rate of 35.5% (11/31), and no differences in revealing the number and the shunt levels of BPS existed between DSA and MSCTA. The number of abnormal systemic arteries demonstrated by MSCTA was quite the same as the number detected by DSA. No false positive case was seen. Conclusion MSCTA is very effective in diagnosing high-flow BPS locating at sub-segmental (or above) pulmonary artery branches as well as in revealing abnormal bronchus-related systemic arteries, which is very helpful in preoperatively evaluating the severity of the BP shunting and the supplying arteries to be obstructed.

Objective To study on gastric varices bleeding treated by tissue adhesive, D-TH glue injection. Methods Fifty-nine consecutive cirrhotic patients with gastric varices were undergone endoscopic D-TH glue injection with sandwich approach. Among these 59 patients, bleeding was present in 46 patients (acute bleeding in 37, recent bleeding in 9) , no previous of gastric variceal bleeding in 13. Follow-up study on therapeutic results and complications were conducted. Results Ninety-six injections were performed in all patients with a mean of 1. 63 injections (1-2ml) per patient. Recurrent bleeding never occurred in 37 patients with acute gastric varices bleeding 72 hours after injection, and the rale of emergency endoscopic he mostasis was 100 per cent. Thirty (65. 2% ) of 46 patients with bleeding gastric varices were followed for 12 36 months, 24. 9 months on average. Recurrence of bleeding occurred in 2(6. 7% ) , 5 ( 16. 7% ) and 8 (26. 7% ) cases at 3 , 12, and 36 months respectively. The rate of survival was 86. 5 per cent. Besides fever occurred postoperatively in 2 cases, other severe complications never happened. Conclusion Endoscopic D-TH glue injection is a safe and effective method for treating gastric varices bleeding.

Objective To evaluate the application of CT perfusion in the assessment of muscle perfusion in ischemic rabbit hindlimb treated by implantation of bone marrow mesenchymal stem cells.Methods Twenty rabbits were divided into two groups randomly (each n =10).Bone marrow mesenchymal stem cells were injected into the left ischemic hind limbs of animal models of intravenous injection group,with same volume of normal saline injected into the control group.Twenty-eight days later,the collateral circulation and blood vessel neogenesis were examined by Toshiba Aquilion One 320-MSCT and pathological approaches.t test and Pearson test were used to compare the parameters.Results The results show that rAF,rBV,rClearance,rMVD were 1.15 ±0.67,1.19 ±0.32,0.62 ±0.20,and 1.34 ±0.28 in intravenous injection group and 0.57 ±0.17,0.74 ±0.19,2.06 ±0.15,0.62 ±0.19 in the control group respectively.There was significant difference of rAF,rBV,rClearance and rMVD between the intravenous injection group and the control group(t =5.75,5.01,-2.81,6.43 respectively,P ＜ 0.01).There was significant correlation between rBV,rAF,rC and rMVD (r =0.857,0.811,0.615,P ＜ 0.01).Conclusion CT perfusion imaging is a relatively accurate technique to assess changes of muscle perfusion in ischemic rabbit hindlimb treated by implantation of bone marrow mesenchymal stem cells.

Objective To evaluate the feasibility of real time elastosonography in estimating the characteristic of nodules in resected hepatocirrhosis specimens.Methods Thirty-eight reseeted hepatocirrhosis specimens underwent elastosonography.The nodules that have drawn attention were performed elastosonography through rhythmic pressing and releasing the probe by manual form on the liver.The hepatic strain on the region of interest was shown by chromatic scale.To compare nodules rigidity with surrounding hepatic tissues, hepatocirrhosis nodules were classified into hard nodules, medium rigidity nodules, mixture of hard and soft nodules, and soft nodules.All nodules were confirmed by pathology.Results Forty-four nodules of 38 hepatocirrhosis exemplar received real time elastosonography.Of 44 nodules, hard nodules were 18,of which 12 (66.7%) were hepatoeellular carcinomas, 2 (11.1%) were dysplasia nodules, 4 (22.2%) were regenerative nodules.Medium rigidity nodules were 7,all were regenerative nodules.Mixture of hard and soft nodules were 11, of which 8 (72.7%) were hepatocellular carcinomas, including 4 accompanied necrotic tissue, 1 (9.1%) was dysplasia nodules accompanied necrotic tissue, the other 2 (18.2%) were regenerative nodules accompanied necrotic tissue.And soft nodules were 8, of which 4(50.0%) were necrotic nodules, 1 (12.5%) was dysplasia nodules accompanied canceration, 1 (12.5%) was hepatocellular carcinoma,2(25.0 %) were regenerative nodules.Conclusions Real time elastosonography can effectively evaluate the comparative rigidity on hepatoeirrhosis nodules,and thus may have potential usefulness on estimating the characteristic of hepatocirrhosis nodules.

Objective To investigate the significance of non-bronchial systemic collaterals (NBSCs) in supplying bronchial hemoptysic lesions,and to study the morphological features of bronchial artery (BA) when NBSCs become the predominant supplying vessels. Methods Multi-slice helical CT angiographic findings in 124 patients with bronchial hemoptysis were retrospectively analyzed. 3D reconstruction of thoracic systemic arteries,including BAs and NBSCs,was performed at the console work station with the help of real-time thin-slice enhanced helical CT scanning. The number of NBSCs and BAs was calculated,and the internal diameter of the arteries and the thickness of pleura in the vicinity of the pulmonary lesion were measured. According to the presence or absence of NBSCs,the patients were divided into NBSCs group and non-NBSCs group. The relevant data was statistically analyzed. Results NBSCs group included 36 cases,the mean internal diameter of BA was (1.850 ? 0.631)mm and the pleura adjacent to the pulmonary lesion was obviously thickened in 22 cases (61%) with a thickness of 2.7-16.0 mm [mean(7.71 ? 4.12) mm]. In the non-NBSCs group (n = 88),the mean internal diameter of BA was (2.200 ? 0.528) mm and the pleura adjacent to the pulmonary lesion was obviously thickened in 7 cases (8%) with a thickness of 1.1-2.4 mm [mean(1.7 ?0.53) mm]. The differences in both internal diameter of BA and the thickness of lesion's adjacent pleura between two groups were statistically significant (P

Objective To compare the variations of serum cytokines before and after ozone injection with different dosages and between intra-articular injection of knee joint and autohemotransfusion injection in experimental rabbits,to make a further understanding of the biologic mechanism of ozone therapy on osteoarthritis(OA).Methods Thirty-six New Zealand white rabbits were equally and randomly divided into six groups:(1) normal group,(2) OA model group,receiving no treatment,(3) group L10,receiving 10 ?g/ml ozone per intra-articular injection,(4) group L30,receiving 30 ?g/ml ozone per intra-articular injection,(5) group S10,receiving autohemotransfusion with 10 ?g / ml of ozonized blood per injection and(6) group S30,receiving autohemotransfusion with 30 ?g/ml of ozonized blood per injection.OA models were prepared by injecting 0.5 ml of collagenase II solution into the knee joint space two times with an interval of three days.Blood samples of all groups were collected 4 weeks after the last ozone treatment for further analysis.Serum levels of NO,T-SOD,TNF-? and IL-1? were estimated.Results The serum levels of NO,T-SOD,TNF-? and IL-1? in the model group and all treated groups were significantly higher than that in normal group(P0.05).Conclusion(1) An increase in the serum levels of NO,T-SOD,TNF-? and IL-1? exists in rabbit OA models.(2) Ozone has no inhibitary effect on the serum levels of NO,T-SOD,TNF-? and IL-1?,regardless of what route of administration of ozone is adopted.(3) The effect of "oxidative preconditioning" is not local,but rather systemic.