Objective:The separation effect for the enantiomers of ketoprofen on four diferent chiral columns was investigated and established a sensitive and stereospecific HPLC separation and determination method for ketoprofen enantiomers in animal plasma.Methods:The enantiomers of ketoprofen was resolved in the normal phase mode by using n-hexane∶isopropanol∶TFA(80∶20∶0.02)as mobile phase with UV detector at a wavelength of 254 nm.The flow rate was 1.0 ml/min.Results:Linear calibration curves were obtained in the range 0.1～10?g/ml for R-(+)-ketoprofen(r=0.999 8)and S-(+)-ketoprofen(r=0.999 6)in plasma.The detection limit(S/N=3) was 2 ng for each enantiomer.Conclusion:This method is simple,fast and can be used to detect optical purity of S-(+)-ketoprofen and monitor pharmacokinetic studies of ketoprofen enantiomers.

Congenital heart disease is a type of common birth defect and most can be cured by surgery with prominent prolongation of life expectancy.But,different kinds of arrhythmias accompanied by congenital heart disease,especially late onset postoperative arrhythmias have become one of the major causes of mortality in these patients.Management and effective treatment for fast and slow arrhythmias associated with congenital heart disease are quite critical.

OBJECTIVE:To investigate the relationship between the perioperative application of antibiotics in caesarean opera-tion and neonatal hyperbilirubinemia,and to provide reference for reducing the incidence of neonatal hyperbilirubinemia. METH-ODS:264 cases of cesarean section women were randomly divided into control group and observation group,with 132 cases in each group. Control group was given antibiotics after operation,and observation group was given prophylactic application of antibi-otics before operation,and equivalent dose of intravenous injection after operation. Postoperative maximum and average tempera-ture,white blood cell(WBC),neutrophils(NEUT),complications and economic index were compared between 2 groups after op-eration. RESULTS:There was no statistically significant difference in postoperative maximum and average temperature,WBC count,NEUT proportion,wound infection and fever rate between 2 groups (P>0.05). The costs of medication,drug dosage per capita,hospital stay and the incidence of neonatal hyperbilirubinemia in observation group were significantly lower than in control group,with statistical significance (P<0.05). CONCLUSIONS:The perioperative standard use of antibiotics in caesarean opera-tion could effectively reduce the incidence of postoperative neonatal hyperbilirubinemia and hospitalization cost,and cut down treat-ment time. It also has a high economic value and worthy of promotion.

Objective:To explore the relationship among the cancer-related fatigue,coping styles and quality of life in breast cancer patients with chemotherapy.Methods:A total 120 female breast cancer patients were selected as the subjects.The instrument used for data collection was a questionnaire that was composed of four parts:the demographic data form,the Revised Piper Fatigue Scale (PFS),Jalowiec Coping Scale (JCS),Quality of Life Questionnaire-Core 30 (QLQ-C30),and Quality of Life Questionnaire-Breast 23 (QLQ-BR23).The statistical software SPSS was used for data analysis.Cancer-related fatigue,coping styles,quality of life and their related factors were determined by nonparametric tests.Spearman correlation coefficient was used to investigate the relationship among cancer-related fatigue,coping styles and quality of life in breast cancer patients with chemotherapy.Results:There was a significant negative moderate correlation between cancer-related fatigue and the quality of life (r＝-0.24,P<0.01),and negative correlation between cancer-related fatigue and the total health status (r＝-0.39,P<0.01).The positive coping styles including optimistic and confronting were negatively correlated with cancer-related fatigue (r＝-0.25,-0.18，Ps<0.05),and were positively related to the functional domain of quality of life(r＝0.35，0.21， Ps<0.05).The positive coping styles including emotive,fatalistic,and palliative were positively correlated with cancer-related fatigue (r＝0.25,0.18,and 0.28， Ps<0.05),and were negatively correlated with the functional domain of quality of life (r＝-0.40,-0.32，Ps<0.05),and emotive and fatalistic were positively related to the symptomatic domain of quality of life(r＝0.40,0.28,Ps<0.05).Conclusion:There exists a certain degree of correlation among cancer-related fatigue,coping styles and quality of life in breast cancer patients with chemotherapy,which may guide the clinical care.

With a high success and low recurrence,catheter ablation has become the standard of cure in children with tachycardia.With the innovation of mapping ablation techniques,catheter ablation for children with tachycardia has been developed.In this review, green cardiac electrophysiology,complex arrhythmias ablation with electroanatomic mapping systems and cryoablation in children will be discussed.

Objective To investigate the relationship between plasma lysophosphatidic acid(LPA) concentration and progression of aortic atherosclerotic lesions for hyperlipidemic diet fed infantile rabbits.Methods Two-month-old infantile rabbits(0.9-1.1 kg) were randomly assigned to normal diet group(group A,n=6) and hyperlipidemic diet group(group B,n=11).Blood lipid levels,LPA,nitric oxide(NO),malondialdehyde(MDA) and superoxide dismutase(SOD) concentrations were taken regularly.The thoracic aorta was taken from a hypertipidemic diet rabbit for pathological study at certion interval of time.Results Electronic microscope analysis revealed subtle changes of aortic wall during 1-2 weeks of the experiment.Fatty streaks(FS) appeared after 4 weeks of hyperlipidemic diet.The degree of FS increased gradually thereafter.Blood lipid levels increased significantly after 1 week of hyperlipidemic diet.Plasma LPA concentration reached its peak level before the appearance of FS.The NO,MDA and SOD concentrations of group B changed significantly proportional to the development of FS.Conclusion Measuring plasma LPA concentration,combined with serum NO,MDA and SOD concentrations can accurately reflect the formation and progression of FS.

Objective To investigate the association between -45C→G mutation at promoter of human urate transporter 1 gene and primary hyperuricemia. Methods The allele frequency and genotypo distribution of -45 C→G mutation at promoter of human urate trans-porter 1 gene were determined by PCR-RFLP in 217 patients with primary hyperuricemia and 419 normal controls. Results The frequencies of the G allele and CG genotype at promoter of human urate transporter 1 gene in patients were significantly higher than that in normal controls (P = 0. 031, P = 0.031). The levels of serum uric acid (UA) and triglyceride (TG) in subjects of CG genotype were significantly higher than those in the objects of CC genotype(t=3.058, t=3.699, P=0.002, P<0.001). There were no significant difference in the levels of total cholesterol (TC), fasting plasma glucose (FPG), urea nitrogen (BUN), creatinine (Cr) between the two groups (P>0.05). Conclusion The -45 C→G mutation at promoter of human urate transporter 1 gene may be related to primary hyperuricemia.

Objective To study the effects of industrial wastewater containing chromium from Anyang Bicycle Works (ABW) on the mutagenicity of ground water and drinking water. Methods The ground water samples were collected from wells located on the southwest of the pollution source of ABW, near the gate of ABW(A) and at a distance of 60 m from the east of ABW(B) respectively, and also from the following sampling points closely located along the lower reach of sewage ditch: cigarette work(C), iron alloy works(D), match works(E), chemical and chemical fiber works(F). The raw water and finished water samples were collected from water plant. The contents of chromium(Cr6+) in water samples were measured and the mutagenicity of the water samples were tested by Vicia faba root tips cell micronucleus test. Results The contents of Cr6+ in ground water samples showed a decreaseing trend (0.358 5-0.010 0 mg/L) with the increasing distances from the sampling points to the pollution source of ABW, the order of which was the following: A, B, C, D, E, F. The contents of Cr6+ in ground water samples collected from the gate of ABW and the location at a distance of 60 m from the east of ABW exceeded the related standard 6.17 and 2.08 times respectively.The contents of Cr6+ in raw water and finished water samples were 0.0123 and 0.0095mg/L respectively.All of the water samples except that from the chemical and chemical fiber works showed higher micronucleus rates in different degree compared with that of negative control. Statistically significant higher micronucleus rate was only observed in water sample from match works compared with that of negative control (P

Objective: To recognize the characteristics of anemia in patients with drug-associated renal parenchymal ARF and to investigate the possible relations among Hb, serum level of EPO and the renal tubulointerstitial injury. Methods: Sixteen in-patients with drug-associated renal parenchymal ARF (including ATN and ATIN groups)for the last five years and 8 healthy volunteers (control group) were assessed in this study. The general information of these patients was analyzed retrospectively. Anemia related laboratory parameters, serum EPO, serum creatinine (Scr) and the renal tubular function from the samples on the time of renal biopsy in each group were compared. For pathological comparison of renal tubulointerstitial pathological changes, renal biopsy specimens from 5 patients with mild non-IgA mesangial proliferative glomerulonephritis were used as negative controls. The pathological changes in renal tubules and interstitial area were semi-quantitive scored by a computer imaging system. The correlations between Hb and EPO levels, as well as EPO level and renal function or pathological injury index were analyzed, respectively. Results: There was no significant difference among the ages, genders and the time intervals both from the administration to the onset of the disease and from the onset to the kidney biopsy among ATN and ATIN groups. Scr level was all higher than that in the control group (P

Objective To make sure the proper time when using self-designed hemostatic belt to press vein after mainline. Methods Divide the subjects into 8 groups, using different pressing time to different group, and then observe the condition of petechia. Results There are significant differences in the different groups about the condition of petechia, P