OBJECTIVE To reduce the career′s risk factors against the physical damage in supply room.METHODS The risk factors in the supply room were analyzed,and the corresponding preventive measures were develop.RESULTS Strengthening education on occupational safety protection and strictly implementing measures could reduce occupational hazards.CONCLUSIONS For different risk factors in the supply room job,appropriate occupational protection measures can effectively reduce occupational hazards.

Objective To explore the interactions mechanism of job embeddedness of nurses with psychological capital,organizational commitment,intention to stay and their influencing factors.Methods By convenient sampling method,722 nurses were investigated with Job Embeddedness Questionnaire, Psychological Capital Questionnaire,Organizational Commitment Questionnaire and Nurses′Intention to Stay Questionnaire.Path analysis was made on relations between the variants.Results Night shift intervals pose positive influence on nurses′psychological capital,organizational commitment and intention to stay.Psychological capital can influence job embeddedness by both directly and indirectly,and can influence their intention to stay indirectly.Organizational commitment can influence job embeddedness strongly and directly (r = 0.494 ),and their intention to stay both directly and indirectly.Job embeddedness can influence their intention to stay directly (r=0.453 ).The model fitting is found desirable.Conclusions Nursing managers can enhance nurses′organizational commitment by developing the psychological capital to enhance nurses′organizational commitment effectively.Nurses with higher level of psychological capital and organization commitment will embed into their workplace more deeply, then increasing their intention to stay ultimately.

Objective To observe the distribution features of phosphorylated p 44/42 extracellular signal-regulated kinase(p-ERK) induced by affective pain in the anterior cingulate cortex (ACC) of the rat.Methods Twelve male SD rats were randomly divided into the control group (n=6) and spinal nerve ligation (SNL) group (n=6).The rats of the SNL group were exposed to unilateral L5 spinal nerve ligation (SNL) surgery.The behavior was examined by mechanical pain thresholds (MPTs) and the pain affect was measured using open-field test, elevated zero maze.The expressions of p-ERK in ACC (AP 3.2,2.7,2.2mm from Bregma) were detected with immunofluorescence test .Results In comparison with the control group , the unilateral mechanical pain threshold in the SNL group decreased significantly after operation . The operation also resulted in pronounced anxiety-like behavior .The p-ERK labelled cells in different levels of ACC ( AP 3.2,2.7,2.2mm from Bregma) of SNL rats were (11.89 ±2.57),(32 ±4.67 ) and (17.56 ±2.04 ), respectively, while the control group rats were (12.44 ±2.16),(10 ±0.87 ) and (10.11 ±1.36).These data indicated that compared with the control group, the expressions of p-ERK in ACC(AP 2.7,2.2mm from Bregma) increased significantly in the SNL group after operation(P<0.01), besides for the level of ACC(AP 3.2mm from Bregma) (P>0.05).Conclusion The results suggest that the neuropathic pain can result in anxiety-like behavior and increased expressions of p-ERK in ACC of the rat.This change may be closely related to the expressions of p-ERK in ACC(AP 2.7,2.2mm from Bregma), but not to the level of ACC(AP 3.2mm from Bregma).

Objective To explore the relationship between mechanical withdrawal threshold ( MWTs) and local cutaneous blood perfusion ( BP ) in rats with acute inflammation induced by carrageenan.Methods Twenty male Sprague-Dawley ( SD) rats were randomly divided into control and model groups.The acute inflammatory rat model was established by subcutaneously injecting with carrageenan into the left hindpaw.MWTs were measured by Dynamic Plantar Aesthesiometer 37450 before injection ( as base) of carrageenan and at 4 h, 24 h, 48 h, 72 h after carrageenan injection. The local cutaneous BP was detected by Pericam Perfusion Speckle Imager ( Pericam PSI) at the time after measuring of MWTs.The above two behaviors were compared and the relationship between them was analyzed.Results MWTs of the model group rats were decreased while BP significantly increased than that in the control group ( P<0.01 ) .The MWTs and BP in the model rats showed a negative correlation, especially the correlation index showed significant differences at 4 h and 72 h after carrageenan injection ( P<0.01) .Conclusions Carrageenan-induced acute inflammation in rats causes significant changes in mechanical pain threshold, which has a negative correlation with local cutaneous blood perfusion.

[Objective]To observe the intervention of Electroacupuncture at Point Jianyu(LI15) on Rabbit Model of Scapulohumeral Periarthritis for receiving possible mechanism.[Methods ]Thirty healthy rabbits(male or female) were randomly divided into three groups:scapulohumeral periarthritis model group,EA Jianyu group and Ibuprofen control group.Each group respectively were detected the concentrations of serum PGE2 before model and treatment and after treatment,and then killed them to achieve tendons of shoulder which were detected the concentrations of Hydroxyproline.And achieve tissues around shoulder which were studied by HE to investigate the changes of the histology.[Results]The concentrations of serum PGE2 had no notable difference between EA Jianyu and model group,Ibuprofen control group and model group after treatment.But it was lowered after treatment than before treatment by EA Jianyu.There were no significantly difference among the three groups about concentrations of Hydroxyproline in tendons,but Ibuprofen control group was higher than EA Jianyu group.Tissues of the shoulder were observed that EA Jianyu restrained reaction of inflammation and degenerescence of tissues,and promoted it better.[Conclusions] EA Jianyu has anti-inflammatory effect and cuts back on the degree of degenerescence of tissues on scapulohumeral periarthritis model in rabbit.

[Objective]The intervention of electroacupuncture (EA) on chronic inflammation and cyclooxygenase-1/-2 mRNA expressions in rats with II collagen-induced arthritis was observed in vivo, so as to discuss the effect of EA on chronic inflammation and its pivotal mechanism. [Method]Wistar rats immunized intradermally with bovine type II collagen, were served as the animal models of chronic inflammation. EA stimulation, begun on day 11 after the injection, was applied at the acupoint equivalent to ST36 for continuous 10 times. Indomethacin and refecoxib were used respectively as the control treatments of NSAID inhibitor and COX-2 selective inhibitor. The arthritic index, swelling volume of paw and concentrations of prostaglandin E_2 (PGE_2) of local tissue were investigated. Furthermore, we investigated the impact of EA on the levels of COX-1 mRNA and COX-2 mRNA in synovial tissues and gastric mucosa of inflammatory rats by using reverse transcriptase-polymerase chain reaction (RT-PCR). [Results]All collagen-injected rats developed chronic inflammation of paws. EA remarkably reduced the paw swellings of rats on day 16 and day 21 and attenuated the severity of arthritis from the day 14 to day 21. The levels of PGE_2 in the inflammatory tissues of swollen paws were also lowered by EA. EA stimulation obviously down-regulated the expression of COX-2 mRNA in synovial tissue, but did not influence the level of COX-1 mRNA expressed in gastric mucosa. Indomethacin and refecoxib also strongly down-regulated COX-2 expression in synovial tissue. However, either indomethacin or refecoxib strongly affected the COX-1 mRNA expressions in gastric mucosa. [Conclusion] EA has a pronounced anti-inflammatory effect on collagen-induced arthritis, and the pivotal mechanism and also the advantage of its therapeutic result may be due to selective suppression of COX-2 gene activation, without destroying COX-1 activity in stomach.

Objective To find a method for real-time measurement of epicardial electrical activity suitable for clinical application.Method Combined unipolar and bipolar mapping technique for recording epicardial electrical activity was applied to detect the propagation of depolarization wave over the epicardium.Epicardial signals from 4 pigs were sampled simultaneously during sinus rhythm or atrial fibrillation(AF).Result Active isopotential map showing the spread of excitation on epicardial surface was demonstrated and correlation analysis data were obtained.The difference in correlation coefficients between adjacent sites was evident during sinus rhythm(above 0.8) and AF(about(0.5).) Conclusion The result shows that combined unipolar and bipolar technique improves the electrode efficiency and the precision in epicardial mapping.Distribution of depolarization wave or the reentry pathway during complex arrhythmia may be revealed by correlation analysis.

In the present paper,the authors review progresses of researches on the protective effect of acupuncture in resisting acute myocardial ischemia-reperfusion injury in patients and animals from 1) resisting injury of oxygen-derived free radicals,2) reducing intracellular Ca2+ overload,3) relieving dysfunction of vascular endothelial cells of the myocardium,4) regulating immune functional activity and reducing inflammatory injury,5) reducing cardiocyte apoptosis,6) upregulating the expression of heat shock protein(HSP 70) in the myocardium,and 7) regulating activities of multiple cardiovascular protection factors and intracellular signaling pathways.The abovementioned results are mainly obtained from experimental animal studies.For this reason,the authors hold that the coming researches on acupuncture therapy for cardiovascular disorders should pay more attention to its clinical application,for example,controlled blood-pressure decline induced lower perfusion of the coronary artery,cardiac surgery(percutaneous tranluminal coronary angioplasty,intracardiac operation underdirect vision) etc.

Objective:To evaluate the value of adjuvant radiotherapy for stage Ⅱ endometrial cancer after surgery.Methods:40 patients with stage Ⅱ endometrial cancer with over five years follow up were analyzed vetrosectively.27 cases received subradical hysterectomy,and additional pelvic lymph node dissection in 13 cases.6 cases were treated with surgery alone.34 cases were treated with postoperative pelvic irradiation and additional vaginal brachytherapy in 12 cases.Results:The 5 year survival rate,pelvic failure rate and extrapelvic recurrence rate were 65%(26/40),17.5%(7/40),25%(10/40) repectively.The 5 year disease free survival rate,pelvic failure rate and extrapelvic recurrence rate were 70.5%(24/34),5.9%(2/34),29%(10/34) respectively in 34 patients with postoperative radiotherapy.5 cases with surgery alone recurrenced at the vaginal residual within two years.The late complications were 44%(15/34),including proctitis,cystitis and intestinal obstruction.Conclusion:Postoperative radiotherapy for stage Ⅱ endometrial cancer can improve the tumor local control and increase the late complications.

OBJECTIVE To understand the variance of antimicrobial resistance and the mutations of resistant genes of Mycobacterium tuberculosis isolates in Haiyan area and to be helpful for infection therapy. METHODS For the patients with the positive smears of sputum, the M. tuberculosis was isolated from by sputum. The antimicrobial resistance of the isolates was detected by high and low concentration of antimicrobial agents method. The resistant-genes mutations of rpoB ane katG genes were detected by real-time PCR. RESULTS Among the 131 strains of clinical M. tuberculosis isolates, the total antimicrobial-resistant rate was 14.5%. In the patients after 2 month therapy, the total antimicrobial-resistant rate was 37.8%. The mutations of rpoB ane katG genes were 14.5% and 8.4% in the initially isolated strains, but 39.2% and 25.7% in the strains after 2 month therapy. CONCLUSIONS The drug-resistance of M. tuberculosis isolates in this area existsd before therapy of anti-infection, and,it can increase therapy time. It is important to detect the resistance and the resistant-genes mutations of the isolates.