1.Technology Verification for Irbesartan and Hydrochlorothiazide Tablets
China Pharmacist 2015;(3):490-492,493
Objective: To verify the feasibility and reliability of the pilot process of irbesartan and hydrochlorothiazide tablets. Methods:Three batches of the tablets were produced according to the determined formula and process, and the key parameters in the pilot production were verified. Results:All the indices for each batch of irbesartan and hydrochlorothiazide tablets conformed to the re-quirements of the quality standard. Conclusion: The pilot process of irbesartan and hydrochlorothiazide tablets is feasible, and the technological process and parameters can ensure the product quality with promising reliability and stability.
2.Characteristic of hyperuricemia in patients with type 2 diabetes and association between serum uric acid level and components of the metabolic syndrome
Journal of Chinese Physician 2008;10(11):1447-1449
Objective To evaluate the clinical and biochemical characteristics of type 2 diabetics with hyperuricemia and to investi-gate the association between serum uric acid and the number of metabolic syndrome components. Method In the cross-sectional study of 547 type 2 diabetics, we recorded body mass index (BMI), systolic blood pressure(SBP), diastolic blood pressure (DBP), and collected blood samples to measure serum uric acid (UA), fasting plasma glucose (FPG), glycosylated hemoglobin (HbAlc), creatinine (Cr) and blood lipid profile, including tfiglyceride (TG), total cholesterol (TC), low-density cholesterol LDL-C) and high-density cholesterol (HDL-C). Results Compared with the normal UA group, the diabetic patients in the high UA group had significantly increased levels of BMI, SBP, DBP, FPG, Cr, TG, TC and HbA1c (P <0.05, P <0.01, P <0.05, P <0.01, P <0.01), and decreased level of HDL-C(P < 0.05). There was no significant difference in the level of LDL-C between the two groups (P > 0.05). According to their possession of 1, 2, 3 or 4 components of metabolic syndrome, 547 cases were divided into four groups (MSI, MS2,MS3 and MS4). There were significant difference in the levels of UA, BMI, SBP, DBP, Cr, TG, TC, LDL-C and HDL-C among MSI, MS2, MS3 and MS4 (All P < 0.05). Multiple regression analysis showed that UA concentration was positively correlated with sex, TG and Cr (Beta:0. 088,0. 350, 0. 124; P < 0.05, P <0.01, P <0.01) and negatively correlated with HDL-C and HbA1 c(Beta: -0.107, -0.124 ; P <0.01, P <0.01). The con-centration of UA was strongly correlated with serum TG. Conclusions Serum UA level in type 2 diabetics was significantly elevated as the number of metabolic components increased. Abnormal TG had great influence on serum UA. Hyperuricemia was considered to be a dangerous factor of the metabolic syndrome.
3.Clinical Significance of blood lactic acid dynamic monitoring in the prognosis evaluation of critically ill pediatric
Jianjiang CHEN ; Xiaomei HUANG
Chinese Journal of Primary Medicine and Pharmacy 2011;18(1):41-43
Objective To study the correlation between blood lactic acid level and PCIS scroing system.To study the relationship between dynamic lactic acid monitoring indexes and prognosis of critical patients.Methods Differences of mortality,incidence of MODS and shock,PCIS score between two groups were compared.The patients were divided into 3 groups by PCIS score to compare pathogenetic conditions prognosis, then to study the were divided into death group and survival group by the hospital prognosis. Differences of their lactic indexes and other clinical indicators which reflected organ/system status were compared, prognostic-significant lactic indexes were selected by mu fti-variable logistic analysis. Results Patients with elevated lactic acid level had more incidence of shock and MODS, higher PCIS scores and higher mortality, the differences had statistical significance compared with the normal lactic acid group. There were significant linear correlation between ICU lactic acid level, peak lactic acid level and PCIS scores respectively. For the patients with elevated lactic acid level, peak lactic acid level, 12h lactic clearance had significant correlation with prognosis. Conclusion PCIS scoring system had a significant linear correlation with lactic acid level. For the patients with elevated lactic acid level,peak lactic acid level,12h lactic clearance were good indicators to evaluate patients' prognosis.
4.Factors Related with Understanding of Stroke in Patients
Chinese Journal of Rehabilitation Theory and Practice 2007;13(3):288-289
Objective To investigate the factors that influence the patients to understand the stroke they suffering from.Methods 61 stroke patients were investigated with a questionnaire designed by ourselves.ResultsThe age,sex and educated level affected the stroke patients' understanding,but the time after onset,the degree of locomotory injured and hemiplegic side did not.ConclusionDistinguishing direction of rehabilitation should be established according to the age,sex and educated level of patients.
5.Analysis of clinical application of laparoscopic-assisted vaginal radical hysterectomy and pelvic lymphadenectomy in 63 patients with early-stage cervical cancer
Jirong WU ; Xia CHEN ; Xiaomei PENG
Chinese Journal of Primary Medicine and Pharmacy 2013;20(16):2427-2429
Objective To investigate the clinical effect and safety of laparoscopic-assisted vaginal radical hysterectomy (LAVRH) and pelvic lymphadenectomy in early-stage cervical cancer.Methods The clinical data of 126 patients with cervical cancer were retrospectively analyzed.63 cases received LAVRH surgery were considered as study group,and 63 cases received laparotomy were selected as control group.The operation time,intraoperative blood loss,intestinal function recovery time,the average hospitalization days,surgical complications,recurrence incidence,etc were compared between the two groups.Results The operation time,intraoperative blood loss,intestinal function recovery time,the average hospitalization days had significantly statistical differences between two groups(P <0.05).The surgical complications and recurrence rate had no significantly statistical differences between two groups (P >0.05).Conclusion LAVRH and pelvic lymphadenectomy has the advantages of minimally invasive,less blood loss,faster recovery,etc.It is an effective method for the treatment of early-stage cervical cancer.
6.Regulation of proliferation and apoptosis of K562 cells by changes of Ras-ERK pathway induced by simvastatin
Qinfang TAN ; Xiaomei LIAO ; Bi CHEN
Journal of International Oncology 2009;36(12):949-953
Objective To investigate the changes in molecule levels of Ras-ERK signal pathway of K562 cells treated with simvastatin in vitro,and to illustrate that simvastatin inducing the changes in molecule levels of Ras- ERK signal pathway is involved in regulation of proliferation and apoptosis of K562 cells. Methods K562 cells,the chronic myelocytic leukemia(CML) cell lines,were cultured and treated with simvastatin in vitro and proliferation activity of K562 cells was detected by MTT. The changes of apoptosis rate and cell cycle of K562 cells were measured by flow cytometry(FCM). The molecular changes of Ras-ERK signal pathway were analyzed by RT- PCR in transcriptional level. Results The proliferation of K562 cells was inhibited by simvastatin,and G_0-G_1 arrested in K562 cells and significant apoptosis rate was observed with FCM. Most molecules of Ras- ERK signal pathway expressed differentially at transctiptional level. Conclusion Simvastatin probablely inhibit proliferation and induces apoptosis of K562 cells,depending on Ras-ERK signal pathway which is involved in cell proliferation and apoptosis.
7.Effects of fungal elicitors on growth of Dendrobium candidum protocorms
Xiaomei CHEN ; Shunxing GUO ; Zhixia MENG
Chinese Traditional and Herbal Drugs 1994;0(03):-
Objective To study the effects of two fungal elicitors on the growth of Dendrobium candidum protocorms cultured on the solid medium.Methods The medium for propagation of protocorms was selected and the growth curve on that medium was obtained.According to the curve,the two fungal elicitors were added at the different growth stages of protocorms.The fresh weight(FW) and dry weight(DW) of protocorms were measured.Results The medium for propagation of protocorms was 1/2MS+20% potato extract+3% sucrose+0.8% agar.Compared with the control MF23 elicitor added at weeks 11 and 13 could significantly increase the FW by 16.4%(P
8.HISTOCHEMICAL OBSERVATIONS ON THE RENAL TUBULO-TOXICITY OF GOSSYPOL
Kai CHENG ; Xiaomei CHEN ; Shepu XUE
Acta Anatomica Sinica 1989;0(S1):-
Gossypol has been shown to cause a side effect hypokalemia. This study was designed to evalulated the possible role of gossypol in relation to renal potassium loss by using histochemical method for comparative analysis of gossypol and gentamicin, a well known tubulo-toxic agent, as positive control with respect to nephrotoxic demage in rats and guinea pigs. The results indicated that gossypol had obvious effect on reabsorption function by inducing transitional decrease of membrane enzymes activities of renal tubular cells but had no effect as comparable with gentamicin to cause structureal damage of tubular cells.
9.PROTECTIVE EFFECT OF CHITIN ON HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS (HUVECs) INJURED BY OXYGEN FREE RADICALS IN VITRO
Tongpo CHEN ; Xiaomei SONG ; Zhiping LI
Chinese Journal of Marine Drugs 1994;0(01):-
The effect of chitin on human umbilical vein endothelial cells (HUVECs) injured by oxygen free radicals was investigated in vitro. The degree of injury was determined by the amount of malondialdehyde (MDA) and lactic dehydrogenase (LDH) in the culture medium. The results indicated that chitin could reduce the amount of MDA and LDH significantly, showing that chitin could protect HUVECs from oxygen free radicals injury.
10.Inhibitory effect of IL-10 on induced expression of ICAM-l in renal tubular cells is mediated by down regulated activity of nuclear factor kB
Jianliang CHEN ; Xiaomei LI ; Haiyan WANG
Chinese Journal of Nephrology 1997;0(01):-
Objecive To explore the effect of interleukin-10(IL10) on tumor necrosis factor-?(TNF?)- induced expression of intercellular adhesion molecule 1 (ICAM-1 )and related transcription pathway in human renal tubular cells. Methods Human renal proximal tubular cell line HK-2 cell was used as target cell. ICAM-1 was measured by cell ELISA and Northern blot in protein and mBNA levels. Activities of transcriptional factors nuclear factor GB(NFkB) and activator protein 1 (AP-1) were determined by electrophoresis mobility shift assay. Results TNF?-induced ICAM-l expression in HK-2 cells was increased in both protein and mNRA levels(P