In order to accelerate the pace of teaching reform,our school explores the tutorial system of undergraduate course this year,this article emphasize the necessity and beneficial effects of this system in clinical practice.

Diagnostics is the bridge from basic medicine to clinical medicine.For the students contacting the clinic for the first time,we take measures to cultivate the consciousness of showing love to patients and dedicating yourself to work.It is beneficial to cultivate the students'ability in clinical practice.

This paper introduces interns shoule know the importance of interpersonal relationship in clinic practice thoroughly and good interpersonal relationship requires the teacher and medical students to make efforts together.

Doctor-patient communication skills are one of the seven lowest requirements of global medical education.From the characteristic of doctor-patient communication in general medical practice,the paper explores the importance of strengthening the training of doctor-patient communication capability and analyzes the training methods.

OBJECTIVE To study the proteome of inferior colliculus and determinate the region-typical proteins which may be the candidate cause of the Central Auditory Processing Disorders. METHODS The telencephalon was taken as reference, and then identified and quantified the proteome of IC of adult rats with iTRAQ. Those with higher abundance in inferior colliculus than the other three regions were considered as IC-Region typical proteins,which may lead to functional specializations. RESULTS We identified 1937 cytomembrane proteins in total, among which there are 53 IC-Region typical proteins, which may lead to functional specializations of inferior colliculus.We used GO and KEGG pathway to analyze these proteins and then found that these proteins mainly take part in the regulation of neurons development and information integrations. CONCLUSION Our quantitative comparison of inferior colliculus has revealed two candidate proteins, including CaMKII and SV2A, which may play important roles in maintaining the balance of excitatory and inhibitory transmitters release. These proteins may be the candidate proteins for Central Auditory Processing Disorders.

This article reported a case of suppurative meningitis caused by Streptococcus suis infection. The patient is an old man who has been engaged in pig breeding. The common symptoms include fever, headache and disturbance of consciousness. According to the results of magnetic resonance imagine and cerebrospinal fluid examination, the patient was considered purulent meningitis caused by Streptococcus suis type 2. After treatment with penicillin anti-infection and dexamethasone inhibition of immune response, the symptoms improved significantly. Three weeks after the onset of the disease, hearing loss occurred, after six months of follow-up, serious hearing impairment was still left.

This paper explored factors influencing initiatives of clinical teachers in millitary medical university for training clinical undergraduates including work pressure after being transferring to civilian,work pressure,policy orientation,economic pressure,student factor and psychological factor,etc. Meanwhile,this paper explored the incentive mechanism of clinical teachers in millitary medical university for training clinical undergraduates including training and evaluating clinical teach-ing teachers, innovating evaluation criterion and creating sustainable teaching atmosphere (building examples and establishing various incentive systems). All measures taken above was mean to inspire the initiatives of clinical teaching teachers and to ensure the quality of clinical teaching.

Background Most anti-inflammation eyedrops are limited in clinical application owing to multiple adverse effects.A novel peptide GC31 derived from human thrombomodulin has a natural anti-inflammatory activity.Compared with conventional anti-inflammatory eyedrops,GC31 possesses more advantages and potential clinical transforming value.However,relevant study is still lack.Objective The purpose of this study was to evaluate the anti-inflammatory effect of GC31 and the possible mechanisms.Methods Sixty SPF male Wistar rats aged 8-10 weeks were randomized into 6 groups using randomized number table.Non-specific keratitis models were established in 40 rats by intrastromal injection of 10 μl of lipopolysaccharide (LPS) dissolved in PBS.Different doses of GC31 (125 μg or 250 μg) or dexamethason soluble in PBS were sunconjunctically injected in the experimental eyes respectively in the low dose GC31 group,high dose of GC31 group and the dexamethason group,and 10 μl of PBS was used in the same way in the PBS control group.No drug was injected in the model group,and the normal rats were employed as the blank control group.The corneas were examined by slit lamp microscope and were scored based on the criteria of Anand 24 hours after injection.Then the corneas were collected for histopathological examination.Expression of nuclear factor-κB (NF-κB) p65 in the corneas was detected using immunochemistry.Expressions of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) proteins were assayed using ELISA.Real-time PCR was used to detect the expressions of IL-6 mRNA and TNF-α mRNA.The use and care of the experimental animals followed Regulation for the Administration of Affair Concerning Experiment animals by State Science and Techonology Commission.Results A significant difference was seen in the ocular inflammatory scores among the six groups (F =301.238,P =0.000).The inflammatory scores were significantly lower in the high dose of GC31 group than those in the model group (1.85 ± 0.36 versus 2.90± 0.43) (t' =-5.144,P =0.000) ; and the scores in the dexamethason group was lower than those in the high dose of GC31 group(t' =-3.931,P=0.000).Infiltration of inflammatory cells in corneal tissue was milder in the high dose of GC31 and the dexamethason group compared with the model group.The positive response for NF-κB p65 was obviously weaker in the rat corneas in the low and high dose of GC31 groups and the dexamethason group in comparison with the model group.The contents of IL-6 and TNF-α proteins in the corneas were significantly reduced in the low and high dose of GC31 group and the dexamethason group compared with the model group (low dose group:t=-2.626,P=0.009;t'=-2.310,P=0.017.high dose group:t =-3.361,P=0.001 ;t'=-3.151,P=0.002),and the contents of IL-6 and TNF-α proteins in the dexamethason group were lower than those in the high dose of GC31 group (t=-3.361,P=0.001;t'=-3.360,P=0.000).In addition,the expression trend and compared results of IL-6 mRNA and TNF-α mRNA among the groups were similar to those of the IL-6 and TNF-α proteins (all at P＜0.01).Conclusions GC31 suppresses LPS-induced corneal inflammation response by downregulating the expression of inflammatory eytokines.The effect is more dominant in the doses of 250 μg than that in the doses of 125 μg.

Objective To study cytotoxic and antineoplastic effect in vitro of tephroseris kirilowii turez, houlub extract on U266 multiple myeloma cell line. Methods U266 cells were cocultured with the tephroseris kirilowii turez, houlub extract. Cytotoxicity assay was used by CCK-8 detection kit. Cell cycle and apoptosis were determined using flow cytometry(FCM) analysis. Results Extract of tephroseris kirilowii turez, houlub showed strong cytotoxicity against U266 cells.The IC50 was about 3.2 mg/L. After exposure of U266 cells to the drug, the distribution of cell cycle was changed compared with that of the controls. When the durg concentration was 1.25, 2.5, 5 and 10 mg/L. respectively, the pencentages of cells in the G0/G1 phase were decreased with (34.12±0.49) %, (38.06 ± 0.63) % , (27.46±0.61) %, (15.91±0.32) %, respectively, while those in the S phase were increased with (4.98± 0.50) %, (4.01±0.22) %, (4.16±0.15) % and (5.04±0.12) % in G2/ M phase were increased with (50.05 ±1.12) %, (51.27±0.71) %, (51.84±0.73) % and (55.11±0.25) %, respectively, and apoptosis cells were increased. Apoptosis of U266 cells inadose-depadeut manner could be deteted with staining of Annexix V FITC/PI testing through FCM. Conclusion Tephroseris kirilowii turez extract showed strong cytotoxic effect on U266 cells. The antineoplsastic mechanism of the drug can be partly due to its induced apoptotic effect.