Objective To investigate the relationship between histopathological changes of donor liver and those after liver transplantation and explore its effects on prognosis.Methods The histopathological changes of donor liver and post-transplantation live biopsy were observed in 87 cases.Results The percentage of hepatic degeneration at immediate biopsy of donor liver was 100%,the injury of sinusoid endothelial cell accounted for 41.3%,the hepatic steatosis for 18.3%and the necrosis of liver cells for 10.3%.Conclusion Most of the pathological changes of the donor liver during and after liver transplantation are reversible.The damage of the hepatic sinusoidal cells is the important criterion for the observation of ischemia reperfusion injury.Moderate and heavy hepatic steatosis can be used in the liver transplantation. After liver transplantation,if the necrosis of liver cells gradually gets serious,the risk of transplanted liver losing its function gets bigger,so the comparative observation has very important value in diagnosis.

Objective To study the change of neurohormonal factors during CABG and find the effective perioperative treatments for coronary artery disease patients with high-risks. Methods Forty-eight patients were divided into on-pump CABG group(group A, n=23) and off-pump CABG group(group B, n=25). The clinical results were observed and blood samples were collected at six time-points. Angiotensin II(AG II), aldosterone(ALD), atrial natriuretic peptide (ANP), insulin, glucose and cTnI levels in plasma were measured respectively. Results The AG II levels in plasma increased in both groups and reached the peak at 6 h after operation in group A and 3 h after operation in group B, respectively. The peak level of ALD in plasma were at 6 h in group A, and 0 h in group B. The level of ANP in plasma was highest at 0 h in group A, and at the midoperation in group B. The insulin level in plasma was highest at 24 h in group A and at 6 h in group B. The insulin level at 24 h in group A was significantly higher than that in group B. The glucose level reached the peak at 3 h after operation in group A and at 0 h after operation in group B. The levels of AG II, ALD, ANP and glucose in plasma in group A were significantly higher than those in group B at any time-point. The cTnI levels were highest at 24 h in both groups. The cTnI levels in group A were significantly higher than group B at time-points. Conclusion CABG can produce hyperosmotic urinative effects and hyperaldosteronism, which in CABG group is strong and persistent effects due to the hyperosmotic urinative effects and hyperaldosteronism. In OPCAB group is light and short urinative effects mainly due to the hyperosmotic. ACEI, ARB and aldosterone antagonists should be used during the perioperation of CABG. During the early postoperative period, the plasma glucose should be well controlled and GIK solution with magnesium is needed.

objective: To investigate the expression of Schwann cell marker S100 protein in salivary adenoid cystic carcinoma and try to find out the correlation of S100 expression and neural invasion of the tumor. Methods: Samples from 20 cases of adenoid cystic carcinoma(ACC) and 18 cases of mucoepidermoid carcinoma(MEC) were immunohistochemical stained with S100 antibody. Results: S100 was positive in all of the samples of the 20 cases of ACC and negative in all of MEC. Neural invasion was observed in 11 out of the 20 cases of ACC and in 2 of MEC. Conclusion: There may be Schwann cell differentiation in salivary adenoid cystic carcinoma, and it may be the histologic base of neural invasion of the tumor.

Objective To investigate the clinical features of invasive pneumococcal disease (IPD) and the antimicrobial susceptibility of invasive Streptococcus pneumoniae (Sp). Methods The clinical features of 54 children with IPD and the antimi-crobial susceptibility of 54 invasive Sp isolates were retrospectively analyzed from 2009 to 2012. Results The ratio of boys to girls in IPD was 1.35:1. Most of the children with IPD were within 2 years old. Few of the children had the onset in summer. Most of the children were diagnosed with septicemia, followed by meningitis. All of the children had middle or high fever. Forty-four children had an increased white blood cell count and 42 children had an increased C-reactive protein.β-lactam antibiotics was most frequently used in clinics, followed by vancomycin. Most of invasive Sp were resistant to penicillin. Multiple drug resis-tance was common in invasive Sp. Conclusions The incidence of IPD has seasonal difference. The clinical manifestations of IPD are diverse, with septicemia being the most common. The resistant rate to penicillin is high in invasive Sp.

Objective To evaluate the value of serum procalcitonin(PCT ) and C-reactive protein(CRP) analysis in the differenti-ation of the cause of fever in cancer patients .Methods 218 cases with fever enrolled were divided into three groups ,including bacte-rial infection group ,viral infection group and tumor related fever group .The positive rates of white blood cell(WBC) count ,percent-age of neutrophil ,PCT and CRP were determined and there correlations were analyzed .Results The positive rates of WBC count , percentage of neutrophil ,PCT and CRP in bacterial infection group were significantly increased compared to viral infection group and tumor related fever group(P<0 .05) .The positive rate of PCT in tumor related fever group was also statistically significant difference compared to viral and bacterial infection group(P<0 .05) .The sensitivity of PCT was 97 .83% and the specificity of PCT was 83 .33% .Conclusion PCT and CRP can help identify causes of fever in cancer patients .PCT has better sensitivity and specific-ity ,it can help anti-infective and provide experimental evidence for tumor treatment ,and also help determine the disease outcome and clinical deterioration .

Forkhead box A1 (FOXA1) is expressed in various organs,including breast,liver,pancreas,bladder,prostate,colon,ovary,lung and esophagus.The FOXA1 expression is associated with the growth and carcinogenesis of these organs.It is recently demonstrated FOXA1 plays a significant role in different biological processes including cell proliferation,differentiation,devolopment,carcinogenesis and the process of epithelial-mesenchymal transition (EMT) etc.Its expression is positively correlated with ER and PR expression,and it is demonstrate to be one of the most important favorate prognostic factors of breast cancer.

Based on thorough analysis of the present healthcare big data,and present problems found in precision medicine,the paper proposed the implementation approach of precision medicine driven by healthcare big data.Citing the practice of the hospital as an example,the authors centered on clinical data to build a precision medicine knowledge system,and leveraged in-depth data mining to develop integrated analysis technology for precision medicine.These efforts aim at application development of precision medicine series upon a panoramic medicine knowledge display,so as to enhance medical quality and diagnosis efficiency and translated use of healthcare big data in precision medicine.

Objective To investigate the effects of icariin (ICA) on partial vasoactive substances in monocrotaline (MCT)-induced pulmonary arterial hypertension (PAH) rat model.Methods Sixty male SD rats were randomly divided into five groups:normal control group,model control group,ICA low-,middle-and high-dose (20,40,80 mg · kg-1 · d-1) group,12 rats in each group.Except for normal control group,the rats were injected with MCT (50 mg · kg-1 · d-1) to establish PAH model.After 1 week MCT-injection,ICA was given by intragastric administration for 3 weeks according to different groups.Mean pulmonary artery pressure (mPAP) was recorded through catheter connected with Power Lab system.Except for normal control group,the right ventricular hypertrophy index (RVHI) was calculated using formula:right ventricle weight/the weight of left ventricle with septum× 100％.The morphology of lung artery was assessed by HE staining.Concentration of angiotensin Ⅱ (Ang Ⅱ),endothelin (ET),prostaglandine F2α(PGF2α),thromboxane A2(TXA2) and prostacyclin (PGI2) in serum was measured by ELISA kit assay.The protein levels of angiotensin converting enzyme (ACE),cyclooxygenase-2 (COX-2) and thromboxane A2 synthetase (TXAS) were analyzed by Western blotting,expression of ACE,COX-2 and TXAS mRNA was measured by real time RT-PCR.Results Compared with the normal control group,mPAP [(48.5±5.2) mmHg] and RVHI (33.3±3.8)％in model control group were significantly increased (P ＜ 0.05),the morphology revealed there was obvious artery remodeling at distal artery,the contents of Ang Ⅱ,PGFA2,TXA2 in serum were elevated,and ACE,COX-2 and TXAS gene expression was up-regulated in rats treated with MCT.ICA (40,80 mg · kg-1 · d-1) treatment significantly attenuated mPAP,RVHI and pulmonary artery remodeling (P ＜ 0.05),and decreased the contents of serum Ang Ⅱ,ET,PGF2β,TXA2,and PGI2,and inhibited the gene expression of ACE,COX-2 and TXAS.Conclusion ICA decreases the contents of AngⅡ,ET,PGI2,PGF2α and TXA2 in the serum of MCT-induced PAH rats,which may be one of the mechanisms underlying ICA inhibiting PAH.

Objective To understand the effectiveness of medical cooperation in the Affiliated Hospital of Southwest Medical University with a county hospital for providing a reference basis for medical cooperation.

This study was aimed to evaluate the feasibility of wind-warm lung-heat disease (non-severe community-acquired pneumonia, CAP) from the clinical curative effect, hospitalization days, medical expenses and other aspects. This study selected 198 wind-warm lung-heat disease (non-severe CAP) inpatients from January, 2012 to December, 2012 with the conventional therapy in the Respiratory Department, Wuhan Integrated Traditional Chinese Medicine (TCM) & Western Medicine Hospital as the control group. And another 179 wind-warm lung-heat disease (non-severe CAP) inpatients were selected from January, 2013 to December, 2013 with the TCM clinical pathway treatment in the Respiratory Department of the same hospital as the treatment group. Retrospective analysis was used to compare the clinical curative effects, hospitalization days, and medical cost differences. The results showed that TCM clinical pathway for wind-warm lung-heat disease (non-severe CAP) can shorten the hospitalization days and reduce patients’ medical costs, and ensure the clinical curative effects. It was concluded that the standardized TCM clinical pathway had positive effect on wind-warm lung-heat disease (non-severe CAP). Therefore, the formulation and implementation of TCM clinical pathway was of great significance in the treatment of wind-warm lung-heat disease (non-severe CAP).