1.Preparation of Hydroxycampothecinum Microspheres and Its Distribution in Mice
China Pharmacy 2005;0(22):-
OBJECTIVE:To prepare lung-targeting hydroxycampothecinum(HCPT) microspheres and evaluate its in vitro release characteristics and its lung-targeting property in mice.METHODS:The microspheres was prepared by solvent volatilization with polylactic acid as chief excipient.The particle size,entrapment efficiency(EE) and drug loading(DL) of the microspheres were studied and the in vitro release rate was compared with that of the crude drug.12 mice enrolled were injected via vena caudalis with HCPT micropheres or HCPT crude drug,at 30 min,the drug level in plasma and tissues were determined and the relative distribution rates(RDR) were computed.RESULTS:81.6% of the microspheres ranged from 7~ 30 ?m in particle size,the mean diameter was (14.2?3.1) ?m,the EE was 72.36%,the DL was (40.6?3.6)%,and the in vitro drug release parameter T50 was 85 min for microspheres vs.18 min for crude drug.The drug level of the microspheres in lung was the highest at (32.2?2.48) ?g?mL-1 with a RDR of 58.1%;The drug level of the crude drug group in plasma was the highest at (13.52?2.58) ?g?mL-1 with a RDR of 25.24%.CONCLUSION:The prepared HCPT microspheres had the distinctive characteristics of sustained-release and lung targeting.
2.Study of Scavenging Action of Longyanshen on Oxygen Free Radicals
China Pharmacy 2001;0(08):-
OBJECTIVE:To study the scavenging action of Longyanshen on O2 ?- and ? OH.METHODS:Spectrophotometry (wavelength 322nm and 536nm)was used to detect the effects of Longyanshen upon O2 ?- and?OHRESULTS:Longyanshen in concentration of 30g/ml could reduce the level of?OH by 284%,and Longyanshen in concentrations of 15g/ml,30g/ml and 60g/ml could decrease the levels of O2 ?- by 441%,630% and 479% and inhibit the generation of O2 ?- by 489%,589%and 675% in vitro,respectivelyCONCLUSION:The results show that Longyanshen could scavenge and inhibit O2 ?- markedly(P
3.Protective Effects and Mechanisms of Longyanshen on Acute Chemical Liver Injury in Mice
China Pharmacy 2001;0(07):-
OBJECTIVE:To study the protective effects and mechanisms of Longyanshen on chemical liver injury in mice.METHODS:The models of CCl 4 ,TAA and acetaminophen induced acute liver injury in mice were used to study the pro?tective effects of Longyanshen,and relevant parameters were detected and compared.RESULTS:Compared with model group,in Longyanshen group,activity of ALT markedly decreased,the contents of hepatic cyt P 450 ,cyt b 5 increased,the content of MDA in liver tissue significantly decreased,the activities of aniline hydroxylase and aminopurine-N-demethylase increased,the activity of Ca 2+ -ATPase in hepatic cell microsome markedly decreased and the activities of GSH-PX and the content of GSH increased.CONCLUSION:Longyanshen has protective effect on chemical liver injury in mice and the mechanisms may be related to the antioxidative action,inhibiting effect on lipid peroxidation,inducing the activities of hepatic microsomal enzymes and blocking the calcium channel.
4.Advances in supracondylar fractures of the humerus treated by closed reduction and percutaneous pinning fixation
Shuming HUANG ; Jianzhong KONG ; Xiaolong SHUI
Orthopedic Journal of China 2006;0(16):-
[Objective]Supracondylar fracture of the humerus is the most common fracture around elbow in children.In recent years,with the progress of minimally invasive technique,closed reduction and percutaneous Kirschner wire fixation has been widely applied in clinics to reduce the complications and surgical trauma.The relative literature concerning supracondylar fracture of the humerus in children was reviewed to find out the latest progress in classification criteria,the manipulation and reference standard of closed reduction,the configuration and effects of different percutaneous Kirschner wire fixation,and the complications and prevention measures for closed reduction and percutaneous pinning fixation.
5.Effect of Extract of Longyanshen(EL) on Immunodepression Induced by Cyclophosphamide in Mice
Xiaolong KONG ; Weizhe JIANG ; Zizhong LIN
China Pharmacy 1991;0(06):-
OBJECTIVE:To observe the effect of extract of longyanshen(EL)on immunodepression induced by cyclophosphamide in mice.METHODS:The immunodepression was induced by cyclophosphamide,the the different oral doses of EL were given to mice for 30 days,and control group took NS.RESULTS:The different doses of EL could significantly increase the killing function of neutrophil(P
6.Anatomical study and clinical application of minimally invasive internal fixation in the treatment of intra-articular fractures of calcaneum via sinus tarsi approach
Jianzhong KONG ; Licheng ZHENG ; Xiaolong SHUI ; Xiaoshan GUO ; Shi LI
Chinese Journal of Trauma 2009;25(9):822-825
Objective To investigate the anatomical features, operative method and efficacy of internal fixation in the treatment of iutra-articular fractures of caleaneum via the sinus tarsi approach. Methods The pathway, branches distribution and anastomosis of perforating descending branch of peroneal artery were observed on 18 adult cadaveric lower limbs. A sinus tarsi approach was designed. From July 2001 to January 2008, 71 intra-articular calcaneal fractures in 68 patients were treated with open reduction and internal fixation via sinus tarsi approach at lateral sides of calcaneus. According to the Sanders classification, there were 26 type Ⅱ fractures, 32 type Ⅲ fractures and 13 type Ⅳ fractures. Results All patients were followed up for a mean period of 39.3 months (13-85 months), and the fractures were completely healed. There was a significant difference in the length, width and height of the calcaneus, Bohler angle and Gissane angle before and after operation (P < 0.01). According to Maryland Foot Score, the operative effect was excellent in 33 feet, good in 29 feet, fair in 6 feet and poor in 3 feet. Conclusion Open reduction and internal fixation via sinus tarsi approach is an effective method for minimally invasive treatment of intraarticular fractures of the calcaneus, with the advantages of good clinical results and causing minimal damage to soft tissues.
8.Treatment of subtrochanteric femur fractures with minimal invasion dynamic hip plate through percutaneous limited open reduction
Yimin WENG ; Jianzhong KONG ; Jun PAN ; Xiaolong SHUI ; Yongzeng FENG ; Xiaoshan GUO
Chinese Journal of Trauma 2013;(4):325-329
Objective To investigate the possibility,surgical methods,outcome and surgical indications of minimal invasion dynamic hip plate (MIDHP) through percutaneous limited open reduction in treatment of subtrochanteric femur fractures.Methods All cases underwent percutaneous limited open reduction with MIDHP.Duration of operation,intraoperative bleeding volume,length of incision,incidence of intra-or post-operative complications of all case were recorded.X-ray films were reviewed periodically after operation to analyze aspects of fracture displacement,loosening of intemal fixation,screw cutting femoral neck,screw penetrating out of or withdrawing from femoral head,bending or breaking of internal fixation,and fracture healing.Hip joint function was evaluated according to Huang' s criteria.Results Operation lasted for 45-55 minutes (average 50 minutes),showing the incision length of 4.0-5.0 cm (average 4.5 cm) and blood loss of 50-200 ml (average 150 ml).A total of 21 cases were enrolled in the study and were followed up for 6-28 months (average 18 months).In the follow-up,loosening or bending of internal fixation,fracture displacement,screw cut-out,and screw penetrating or withdrawing from femoral head did not occur.In the meantime,incision,bone,and joint were not infected.All cases had bony fusion within 3 months with the fracture healing rate of 100%.According to Huang' s criteria,hip function was excellent in 19 cases and good in two.Conclusions MIDHP with percutaneous limited open reduction is characterized by less trauma,small incision,less blood loss,less postoperative complications,firm fixation,early functional exercise,free load,and good hip functional recovery and hence is suitable for cases of different subtrochanteric femur fractures,especially for cases combined with osteoporosis.
9.Percutaneous C2 pedicle lag screw for treatment of Hangman's fractures
Yimin WENG ; Huazi XU ; Xiaolong SHUI ; Yan LIN ; Xiangyang WANG ; Jianzhong KONG ; Yonglong CHI
Chinese Journal of Trauma 2008;24(8):612-614
Objective To study the feasibility and clinical effect of percutaneous C2 pedicle lag screw in treatment of Hangman's fracture and define the indications of the technique. Methods There were 9 patients including 5 males and 4 females at average age of 36 years (26-68 years). According to a Levine and Edwards System, there were 5 patients with type Ⅰ hangman' s fracture, 3 with type Ⅱ hangman' s fracture and 1 with type Ⅱ A hangman' s fracture. According to American spinal injury association (ASIA) system, the spinal cord function was ranked at Type D in 2 patients and Type E in 7. All patients achieved anatomic reduction by skull traction. Under general anesthesia, 9 patients were fixed with percutaneons C2 pedicle lag screw. The whole procedure was done under monitoring of "C"-arm fluoroscopy for safety and accuracy. Results All patients obtained bony fusion within 2-3 months, with no infection, neurological deficits, vertebra artery injury or other complications. CondusionsThe percutaneous C2 pedicle lag screw fixation is minimally invasive and effective for treatment of Hangman's fracture. During the course of treatment, the function of upper cervical spine remains unaffected.
10.Radiological study and treatment strategies for complex tibial plateau fracture involving posterior fragment
Xiaolong SHUI ; Yimin WENG ; Xiaozhou YING ; Hua CHEN ; Yongzeng FENG ; Xiaoshan GUO ; Jianzhong KONG
Chinese Journal of Trauma 2014;30(11):1137-1140
Objective To investigate radiological characteristics of the posterior tibial plateau fracture and operative treatments for the fracture.Methods Thirty-one cases of posterior tibial plateau fracture treated between February 2009 and February 2014 were enrolled.There were 19 men and 12 women aged 24-72 years (mean,42.5 years).Injury arose from traffic accidents (13 cases),falls from a height (9 cases),fall on the ground (5 cases),and crash (4 cases).Type Ⅴ in 15 cases and type Ⅵ in 15 cases were classified by the Schatzker classification.Meanwhile,all fractures were identified as three-column pattern.According to the X-ray and CT manifestations of fracture displacement,the cases with minor displacement were grouped as Group A (22 cases) and the cases with major displacement group as Group B (9 cases).Depending on the radiographic characteristics of fracture size and angle off fracture line,anterior approach for reduction and fixation was performed in Group A and combined anterolateral and posteromedial approaches in Group B.Radiographic and functional outcomes were evaluated using the Rasmussen score.Results All cases underwent one-stage surgery uneventfully.Operation time was 80-120 minutes (mean,98 minutes) in group A and was 110-165 minutes (mean,110 minutes) in Group B.Mean total operation time was 105 minutes.Period of follow-up was 6-48 months (mean,21.5 months).Rasmussen radiographic results showed total excellent to good rate of 84% with 86% in Group A and 78% in Group B respectively.Rasmussen functional results showed total excellent to good rate of 87% with 91% in Group A and 78% in Group B respectively.Conclusions Anterior approach or anterolateral approach combined with posteromedial approach for reduction and internal fixation is developed according the radiographic findings and degree of fracture displacement.Clinical outcome is good and associated intraarticular soft tissue injury can be managed concurrently.