OBJECTIVE:To study the scavenging action of Longyanshen on O2 ?- and ? OH.METHODS:Spectrophotometry (wavelength 322nm and 536nm)was used to detect the effects of Longyanshen upon O2 ?- and?OHRESULTS:Longyanshen in concentration of 30g/ml could reduce the level of?OH by 284%,and Longyanshen in concentrations of 15g/ml,30g/ml and 60g/ml could decrease the levels of O2 ?- by 441%,630% and 479% and inhibit the generation of O2 ?- by 489%,589%and 675% in vitro,respectivelyCONCLUSION:The results show that Longyanshen could scavenge and inhibit O2 ?- markedly(P

OBJECTIVE:To study the protective effects and mechanisms of Longyanshen on chemical liver injury in mice.METHODS:The models of CCl 4 ,TAA and acetaminophen induced acute liver injury in mice were used to study the pro?tective effects of Longyanshen,and relevant parameters were detected and compared.RESULTS:Compared with model group,in Longyanshen group,activity of ALT markedly decreased,the contents of hepatic cyt P 450 ,cyt b 5 increased,the content of MDA in liver tissue significantly decreased,the activities of aniline hydroxylase and aminopurine-N-demethylase increased,the activity of Ca 2+ -ATPase in hepatic cell microsome markedly decreased and the activities of GSH-PX and the content of GSH increased.CONCLUSION:Longyanshen has protective effect on chemical liver injury in mice and the mechanisms may be related to the antioxidative action,inhibiting effect on lipid peroxidation,inducing the activities of hepatic microsomal enzymes and blocking the calcium channel.

AIM:To evaluate the ability of dendritic cells (DCs) loaded with tumor lysate to initiate cell mediated immune responses by stimulating naive T cells, and the efficiency of activated T cells to kill autologous tumor cells in vitro. METHODS: The peripheral blood lymphocytes and monocytes were obtained from the advanced renal cell carcinoma patient by eonglutination method. The immature dendritic cells were generated in the presence of interleukin -4(IL-4) and granulocyte/macrophage colony-stimulating factor (GM-CSF) from monocytes of healthy individuals.These cells were pulsed with tumor lysate or not. Induction of tumor-specific cytotoxic T lymphocytes(CTLs) response by mature dendritic cells (mDCs) was evaluated by the CD95(Fas) expression assay through FCM and the cytotoxic assay a gninst autolognns human tumor cells. RESULTS: Human immature dendritic cells and T cells obtained from healthy donors were stimulated with tumor- pulsed dendritic cells. The immature dendritic cells were applied to the cytotoxicity assay a gainst target autologons tumor cells. The CD95 (Fas) expression, IFN-γ, and TNF -α secreted by the CTLs in tumor lysate-plused DC group were higher than those of other groups. The capacity of the CTLs to kill autolognns tumor cells was significantly different(P<0. 05). Antigen-specific DCs vaccine can induce T cells activation and proliferation, thus we can obtain higher proportion of tumor specific cytotoxic T cells(CTLs), and enhance the CTLs to secret IFN-γ and TNF-α. CONCLUSION: Our results indicate that monocyte-derived human dendritic cells pulsed with tumor lysate could in duce the specific antitumor effect against autologons tumors. This in vitro model offers a new and simple approach to the development of DC + CTL - based immunotherapy.

AIM:To evaluate the ability of dendritic cells (DCs) loaded with tumor lysate to initiate cell-mediated immune responses by stimulating naive T cells, and the efficiency of activated T cells to kill autologous tumor cells in vitro. METHODS: The peripheral blood lymphocytes and monocytes were obtained from the advanced renal cell carcinoma patient by conglutination method. The immature dendritic cells were generated in the presence of interleukin-4(IL-4) and granulocyte/macrophage colony-stimulating factor(GM-CSF) from monocytes of healthy individuals. These cells were pulsed with tumor lysate or not. Induction of tumor-specific cytotoxic T lymphocytes (CTLs) response by mature dendritic cells (mDCs) was evaluated by the CD95(Fas) expression assay through FCM and the cytotoxic assay against autologous human tumor cells. RESULTS: Human immature dendritic cells and T cells obtained from healthy donors were stimulated with tumor-pulsed dendritic cells. The immature dendritic cells were applied to the cytotoxicity assay against target autologous tumor cells. The CD95(Fas) expression, IFN-? and TNF-? secreted by the CTLs in tumor lysate-plused DC group were higher than those of other groups. The capacity of the CTLs to kill autologous tumor cells was significantly different(P

OBJECTIVE:To observe the effect of extract of longyanshen(EL)on immunodepression induced by cyclophosphamide in mice.METHODS:The immunodepression was induced by cyclophosphamide,the the different oral doses of EL were given to mice for 30 days,and control group took NS.RESULTS:The different doses of EL could significantly increase the killing function of neutrophil(P

Long non-coding RNA (LncRNA) is a kind of length greater than 200 nucleotides not encode any proteins RNA molecules.Although not encode any proteins,LncRNA through chromatin modification,transcription activation and interference et al.involved in a variety of control process in the cell.In recent years,the study found that the expression of a variety of LncRNA abnormalities in gastric cancer.These changes are closely associated with the development and prognosis of gastric cancer,regulate the expression of related LncRNA can significantly improve the prognosis of patients.Targeted to regulate the expression of LncRNA can provide new strategies for treatment of gastric cancer.

Objective To study the changes of tissue plasminogen activator (t-PA), plasminogen activator inhibitor 1 (PAI-1), and soluble thrombomodulin (sTM) induced by dengue 2 virus (D 2V) infection of vascular endothelial cells. Methods The effects of D 2V infection on the production of t-PA, PAI-1, and sTM of human umbilical cord vein cells were studied. Results D 2V infection significantly induced the secretion of sTM and t-PA but showed no such effects on PAI-1 of human endothelial cells. Antibody against IL-6 inhibited D 2V-induced t-PA production of endothelial cells. A close correlation between serum levels of IL-6 and t-PA was found in dengue hemorrhagic fever (DHF) but not in dengue fever (DF) patients. Conclusion IL-6 can regulate D 2V-induced t-PA production of endothelial cells, suggesting that endothelial cells can be the target for D 2V infection and that D 2V-induced t-PA, TM, and IL-6 production of endothelial cells may contribute to the pathogenic development of dengue haemorrhagic fever/dengue shock syndrome (DHF/DSS).

Objective To investigate the diagnosis,preoperative examination and surgical treatment of carotid body tumor(CBT).Methods From December 2008 to December 2012 80 patients of carotid body tumor were treated in our center.The average age was(43:±:12)years,33 cases were male,47 cases were female.The lesions were unilateral in 68 cases and bilateral in 12.All tumors were surgically resected.In 26 of Shamblin Ⅰ,32 of Shamblin Ⅱ and 19 of Shamblin Ⅲ tumors were simply resected.One Shamblin Ⅱ and 2 Shamblin Ⅲ cases underwent tumor resection combined with external carotid embolization.3 Shamblin Ⅱ and 8 Shamblin Ⅲ cases were treated by tumor resection combined with internal carotid reconstruction,1 Shamblin Ⅲ was done with tumor resection combined internal carotid repair.Results All of 80 patients with 92 tumors were successfully resected.There was no death case during peroperative period,while one case developed hemiplegia and 14 cases suffered cranial nerve injuries.68 cases (85％) were followed-up from 1 year to 6 years.Thera were 14 patients with cranial nerve injuries,2 of them were lost to follow-up,11 with complete or partial recovery and 1 was still doing poorly.Conclusions Tumor size and its relation to the carotid artery form the basis upon which appropriate operation is adopted.

Objective To discuss the MRI features and its clinical significance of bone contusion in knee joint.Methods MR images of injuries of knee in 40 cases were retrospectively analyzed,the complications were reviewed simultaneously.Results 69 lesions of bone contusion in 40 cases were discovered,of them,Ⅰ type was in 38 and Ⅱ type was in 31, in company with fractures were 24 in 22 cases and ligament injury was in 33 cases.Conclusion The present of bone contusion is prompt to the bone injuries,MRI is of significant value in showing bone contusion and its complications in knee.

AIM: The aim of this study is to elucidate the effects of lipopolysaccharide (LPS) on tissue plasminogen activator(tPA) and plasminogen activator inhibitor type-1(PAI-1) expression and secretion in endothelial cells. METHODS: Cultured human umbilical vein endothelial cells (HUVECs) were induced by LPS for different times. Cell viability was then determined by cell counting kit-8. tPA and PAI-1 activities in the media were assayed by fibrin overlay and reverse fibrin autograph, respectively. Cytoplasmic RNA was prepared using the Trizol method and was assayed for PAI-I and tPA mRNA levels by reverse transcript-polymerase chain reaction(RT-PCR). RESULTS: LPS(10 mg/L) did not produce cell toxicity according to LDH determination in culture media. PAI-1 activity in LPS group was high (P0.05). CONCLUSION: LPS (10 mg/L) did not show signs of cell toxicity, but promoted the expression of PAI-1 mRNA and induced an increase activity of PAI-1. However, LPS (10 mg/L) did not change tPA mRNA expression. The time-dependent increase in PAI-1 mRNA expression and activity shifts the local balance toword increased anti-fibrinolytic capacity, which can amplify the extent of acute thrombosis after plaque rupture. This is one of the possible reasons that cause thrombus,blood coagulation and disseminated intravascular coagulation (DIC) during septicemia.