Objective: To investigate the action of tetramethylpyrazines on cardiac myocyte hypertrophy induced by endothelin-1(ET-1). Methods: The blockers of L-type calcium channel(Larcidipine),TMPP and TMPH were used to influence protein synthesis stimulated by chronic ET-1. Results:(ET-1) group's intracellular protein content in cardiac myocyte was(279?39)?g/ml(17% higher than the control group,P

Objective To explore the use of mosapride citrate in treating reflux esophagitis and nursing intervention spitley effect. Methods In 40 cases of reflux esophagitis in patients with routine therapy, and classified as the control group, the other 40 patients in the control group based on the use of mosapride citrate. And classified as the observation group, two groups of patients were in our hospital from June 2015 to January 2017. Results The study in the treatment of the two groups of patients with effective rate of 95.0%, the observation group was significantly higher than the control group 80.0%, between the two groups showed significant differences (P<0.05). The adverse effects of more than two groups found, two groups of patients with adverse reaction incidence was no significant difference, that shows no significant difference. Conclusion Mosapride citrate in reflux esophagitis treatment and intervention effect analysis found that the compared with conventional therapy, can be in At the same time, it can improve the curative effect of the patients, and will not increase the possibility of adverse reactions, so it is worthy of clinical reference.

Objective To explore the influence on the quality of marital and life of the congenital absence of vagina patients with operation by psychological intervention.Methods The congenital absente of vagina patients were divided into psycholOgical intervened group(n=18,all cases were treated with the cognitive and behaviour therapy)and without intervened group(n=11).Before and after treatment the scores of the nottingham health poofity(NHP)were used in the two groups.After treatment the Olson's enrich questionary were tested also.Results The scores of NHP and Olson of psychological intervened group were significantly superior to those without intervened group(P＜0.05 or P＜0 01).Conclusion The cogntive and behaviour theraopy before operation is good for the quality of marital and life in congenital absence of vagina patients.

Objective To investigate the influences of 4 kinds of collagenase with different degrees of activity on the survival rate,contraction and relaxation functions of isolated rats' myocardiocytes.Methods Rats' myocardiocytes were isolated by enzymolysis with 4 kinds of collagenase with different degrees of activity.The survival rate of myocardiocytes was observed immediately after isolation(D),one hour after loading with calcium(E time point) and 10 minutes after electric stimulation(F time point).The contraction and relaxation functions of myocardiocytes,including contraction amplitude(ph),the proportionality of ph/single cardiocyte length(bl),maximal velocity of contraction(+dL/dt) and maximal velocity of relaxation(-dL/dt),were measured with IonOptix video edge tracker.Results From 203U/mg to 299U/mg,with lowering of the activity of collagenase,the isolation time became longer,the survival rate of myocardiocytes declined 1 hour after loading calcium and 10 minutes after electric stimulation(P

Objective:By means of balanced board,we can comprehensively assess the hospital and promote the hospital science progress from four aspects such as patient,finance,interior procedure,and the study and development.Method: Introducing the source content and character of the balanced board;Introducing the reason,qualification,instruction and effect for ChongQing NO 9 Hospital to apply the balance board.Result: We assessed the balance board from finance,interior procedure,patient,study and development: The hospital's operation income structure was more reasonable;the hospital's income is equal to the economic development;the medical staff's quality had been improved;the civilization and brand of hospital were generally promoted.Conclusion:Balance board can be used in the medical treatment and health care reformation.As a management method of hospital it can be combined with the hospital assessment items.When the hospital employs the balanced board,we can combine the management object and management cost,the development of hospital and patients' satisfaction,hospital income and local economical level as well as fee for medical treatment,performance and quality in order to promote the development of hospital.

Objective To assess the relationship between human papillomavirus (HPV) and extrageni-tal Bowen's disease. Methods Regular PCR with consensus primers for LI region as well as mix primers and nested PCR were performed to detect the DNA of a broad range of cutaneous and mucosal HPV types in tissue samples from lesions of 41 patients with extragenital Bowen's disease and from normal skin of 48 human controls. Semiquantitative PCR and tyramide-based in situ hybridization (ISH) were also conducted to determine the load and localization of HPV DNA in HPV-positive samples. Results HPV DNA was detected in lesions from 5 (12%) of the 41 patients with extragenital Bowen's disease. Of the 5 HPV-positive patients, 3 carried mucosal HPV types (HPV16 in 2 cases, HPV 33 in 1 case) with a viral load of 101 to 103 copies, 2 cutaneous HPV types (HPV27 in 1 case and HPV76 in 1 case). As ISH showed, there was a generalized expression of mucosal HPV DNA in most tumor cell nuclei but not in peritumoral normal tissue, and no expression of cutaneous HPV DNA was observed in lesions. HPV DNA was detected in 1 (2.1%) control tissue sample, which proved to be epidermodysplasia verruciformis-associated HPV23. There was no significant difference in the detection rate of HPV DNA between the patients and controls. The viral load of cutaneous HPV types amounted to 10-2 to 10-3 copies in the 2 patients, which was similar to that of HPV 23 in the normal control. Conclusion Mucosal HPV types may be closely associated with the development of extragenital Bowen's disease.

Objective To explore the influence of psychological intervention on quality of life(QOL) in the hysteromyoma patients underwent surgery. Methods Three hundred and twenty patients with hysteromyoma were divided into the psychological intervened group ( all cases were treated with the comprehensive psychological intervention) and the control group (without intervention). Before and after treatment, the scores of the Olson's Enrich Questionary and the Nottingham Health Poofity (NHP) were assessed in the two groups. Results Before surgery, the scores of N HP and Olson were not significantly different between the psychological intervened group and the control group ( P > 0. 05 ). The scores of NHP of total hysterectomy in the control group after surgery were higher than that before surgery,and the scores of Olson were lower significantly ( P <0. 05 or P <0. 01). The scores of NHP of subtotal hysterectomy, myomectomy and blood vessel involvement treatment in the control group after surgery were significantly lower than that before surgery, and the scores of Olson were higher ( P < 0. 05 or P < 0. 01 ). After surgery,the scores of NHP of all patients in the psychological intervened group were significantly lower than that in the control group,and the scores of Olson were higher significantly (P <0. 05 or P <0. 01). There was no significant difference in ovarian hormones level between pre- and post- surgery (P > 0. 05). Conclusions The comprehensive psychological intervention pre- and post- surgery is good for the quality of life in hysteromyoma patients.

Objective To study the relationship between ammonia concentration levels,Child class in cirrhotic patients with helicobacter pylori(Hp) infection and to observe the Hp eradication effect on ammonia.Methods Group A was consisted of 49 cases of cirrhotic patients with Hp infection.Group B included 46 cirrhotic patients without Hp infection.Group C was non-cirrhotic patients with helicobacter pylori infection.Each patient in all three groups was compared with change in serum ammonia concentration before and 4 weeks after Hp eradication therapy.Results The serum ammonia concentration in group A was significantly higher than group B and group C(P0.05).In group A,the serum ammonia concentration was increased as Child classification from A to C(P

Objective To approach the effect of psychological recuperation on the mental health of the medical personnel working in SARS ward. Methods A total of 131 medical workers working in SARS ward were randomly selected as the objects to be psychologically recuperated. Results Both groups' score on somatization, obsessive, interpersonal sensitivity, depression, anxiety and phobia anxiety is higher than that of the local norm before the training, there is no significant difference between the training group and the control group. After the training, there is no significant difference between the training group and the local norm except somatization; the control group's score in somatization, obsessive, interpersonal sensitivity, depression, hostility is higher than that of the local norm, while the training group′s scores in the five dimensions is obvious lower than that of control group. Conclusion Psychological behavior training played a positive role on the' mental health of the medical personnel working in SARS ward.

BACKGROUND:Under certain conditions, stem cel s can be induced to differentiate into vascular endothelial cel s, which can promote the angiogenesis of ischemic lower limbs and the establishment of effective circulation and improve distal blood supply of the ischemic limbs. OBJECTIVE:To review the biological characteristics and pro-angiogenesis mechanism of umbilical cord mesenchymal stem cel s and to investigate the current status of umbilical cord mesenchymal stem cel s in the repair of neuropathy and chronic wounds. METHODS:PubMed, VIP and Wanfang databases were searched for relevant articles published from 2000 to 2015 using the keywords of“stem cel s transplantation, umbilical cord mesenchymal stem cel , diabetic angiopathies”in English and Chinese, respectively. RESULTS AND CONCLUSION:Compared with peripheral blood stem cel s and bone marrow mesenchymal stem cel s, umbilical cord mesenchymal stem cel s are characterized as more widespread sources, easy col ection, stronger amplification ability, no immunogenicity, and no ethical controversy, which have become ideal target and seed cel s for pro-angiogenesis and gene therapy in ischemic diseases. Umbilical cord mesenchymal stem cel s can differentiate into vascular endothelial cel s and fibroblasts involved in wound healing. In addition, these cel s can promote the production and expression of neurotrophic factors, promote nerve regeneration in ischemic tissues, and participate in tissue repair and accelerate healing of ulcers by paracrine and autocrine cytokines, anti-inflammation and immunomodulation. Therefore, umbilical cord mesenchymal stem cel s have a broad prospect in the improvement of diabetic lower limb ischemia, repair of diabetic peripheral neuropathy and promotion of chronic ulcer healing. Compared with stem cel transplantation alone, umbilical cord mesenchymal stem cel s transplantation combined with gene therapy can further enhance cel survival and pro-angiogenesis.