AIM: To investigate the transfection efficiency of mouse liver with non-viral vector containing manganese superoxide dismutase (Mn-SOD) gene. METHODS: The eukaryotic expression vector, gWiz/Mn-SOD, encoding human manganese superoxide dismutase was constructed. The plasmids of gWiz/Mn-SOD were mixed with cationic lipids, followed by injection into mice via branch of superior mesenteric vein, to induce Mn-SOD over-expression in murine liver detected by RT-PCR, Western blotting, SOD activity and immunohistochemical staining. RESULTS: gWiz/Mn-SOD transfection resulted in the obvious expression of exogenous Mn-SOD mRNA and protein in hepatic tissues at 8 hours after injection, and elevated mitochondria SOD activity 8.4 times in transfected hepatocytes than that in non-transfected cells at 72 hours after injection. It was showed that nearly 70% of mouse hepatocytes was obviously Mn-SOD positive after transfection. CONCLUSION: High expression efficiency of Mn-SOD gene in mouse liver is achieved safely by injection of gWiz/Mn-SOD and cationic lipid mixture into branch of superior mesenteric vein. [

Objective To study dose-response relationship for chromosome translocation and its persistence measured in human lymphocytes exposed to 60Co γ-rays.Methods The chromosome translocations in human peripheral lymphocytes were detected by fluorescence in situ hybridization using 4# and 7# combination of composite whole chromosome-specific DNA probes and Giemsa stain.Results The dose-response relationship for chromosome translocation induced by 60Co γ-rays in vitro could be described by the function:Y=0.0030+0.0134D+0.0165D2.The frequencies of chromosome translocations induced by 0.5 and 2 Gy γ-irradiation did not diminish over time,so it exhibited excellent persistence.Conclusions The results indicate that retrospective dose-reconstruction can be accomplished using chromosome translocation frequency.

Objective To predict the prognosis after acute cerebral infarction using a combination of indicators. Methods Two hundred and seventeen patients with acute cerebral infarction admitted from October 2005 to December 2008 were studied. Logistic regression analysis of the data from 112 of the patients admitted from October 2005 to March 2007 was used to select 20 indicators for study. The indicators were combined into prognostic indexes using a multi-layer perception (MLP) neural network (NN) model. Data on the subsequent 105 patients were usedto appraise the model. Results The agreement of the prediction results of the NN model with the real recovery observations was rated as "excellent" in 39 cases, "OK" in 27 and "bad" in 32. The sensitivities were 95.1%,87.1% and 96.9% respectively. On average, the differences between the predicted results with the NN model andthe real recovery were not significant. Conclusion The NN model delivered good precision in predicting the outcome of acute cerebral infarction and it is worthy of further investigation.

ve To explore the hygienic safty of cosmetics for special use. Methods The detection and analysis on the hygienic safety of 432 domestic cosmetics for special use saled in Guangdong provincial markets were carried out in 2000. Results The orders of qualified rates of cosmetics for special use from high to low were as fol-lows: deodorant cosmetics (100%), hair-removing cosmetics(88.89% ), breast massage (80.00% ), antisunburn lotion (77.88%), fading cream(75.68% ). hair nourishment(64.29% ), beautifying cosmetics(50.00% ), hair dye (40.74%) and hair pem(28.57%). The highest unqualified rate of hygienic safety test on cosmetics was 17.28% for Ames test, next was 14.81% for chromosome aberration test on mammalian cells in vitro, 14.13% for eye irritation test and 10.88% for skin allergic reaction. Conclusion The management on the hygienic safety of cosmetics for special use should be strengthened further.

Objective To study the activity of N acetylglucosaminyltransferase Ⅲ, Ⅳ and V in extrahepatic cholangiocarcinoma and its significance.Methods The activities of N acetylglucosaminyltransferase(GnT) Ⅲ, Ⅳ and Ⅴ in extrahepatic bile duct carcinoma (EBDC) of 15 cases were determined by reverse phase HPLC (high performance liquid chromatography), and results were compared with benign biliary diseases in 15 cases. Results Compared with normal control, that the specific activities of GnT Ⅲ and GnT Ⅴ increased by 3 3 and 13 5 fold respectively in all of the EBDC samples(all P

Background Leber hereditary optic neuropathy (LHON) is a maternally inherited disorder characterized by a bilateral acute or subacute painless central visual loss in young adults,predominately in males.So far no one theory can completely explain all clinical manifestations of LHON.Objective This study was to investigate whether there is a linkage between X-chromosomal and mitochondrial mutation in the inheritance of a Chinese LHON pedigree with only male patients.Methods This study was approved by Ethic Committee of Affiliated First Hospital of Zhengzhou University and followed by Declaration of Helsinki.A Chinese LHON pedigree was included in Anyang city from January 2008 to August 2016.Periphery blood of 5-10 ml was collected from 4 sufferers,13 maternal members and 10 non-maternal members for DNA extraction and PCR sequencing.The gene scanning and genotyping analysis were performed by ABI-PRISM 3100 genetic analyzer and Genotyper 3.7 software,and linkage analysis was carried out with Linkage software for the calculation of logarithm of odds (LOD).Mitochondrial DNA (mtDNA) sequence,fluorescence-based Genescan for X-chromosomal sequence were analyzed in the propositus and haplotype was evaluated.Results A total of 5 generations and 71 families were included in the pedigree,with 6 male sufferers,30 maternal members and 41 non-maternal members.The visual acuity was ≤0.10,and the central visual field defection,the optic nerve flushing was found in the acute phase,different levels of the optic nerve fibers atrophy were found in the chronic phase;visual evoked potential (VEP) amplitude was low and peak latency were found in the male patients,and no any ocular abnormality was seen in the maternal members,meeting a maternally inherited characteristics,with the penetranee of 20％.The three primnary mutations were not been found in this family bv PCR sequencing,mtDNA sequencing appeared 31 variation of loci in the proband,including a known G3635A mutation,as well as an unknown ND5 A12340G missense mutation and ND4 T11809C synonymous mutation as well as 28 polymorphism of locus,and the proband was mitochondrial haplotype F1.The maternal families were mutation carriers of G3635A and AI2340G loci,while the same mutation was not found in the normal family members and 107 controls.The maximum two point parametric LOD score was 1.46(θ=0.0) for marker DXS1060,1oeated at Xp22.3,and the two-point and multipoint non-parametric linkage analysis were significant (all at P＞0.05).Conclusions The ND5 A12340G and ND1 G3635A mutations coexist in this LHON family,and the ND5 A12340G mutation is a newly reported mutation.There is no evidence for an X-linked modifiers loci in this Chinese LHON family.

Objective To study the relationship between ammonia concentration levels,Child class in cirrhotic patients with helicobacter pylori(Hp) infection and to observe the Hp eradication effect on ammonia.Methods Group A was consisted of 49 cases of cirrhotic patients with Hp infection.Group B included 46 cirrhotic patients without Hp infection.Group C was non-cirrhotic patients with helicobacter pylori infection.Each patient in all three groups was compared with change in serum ammonia concentration before and 4 weeks after Hp eradication therapy.Results The serum ammonia concentration in group A was significantly higher than group B and group C(P0.05).In group A,the serum ammonia concentration was increased as Child classification from A to C(P

Objective To explore clinical and pathological characteristics of neurological lesions in primary Sj(o)gren's syndrome (pSS).Methods Ten patients diagnosed as pSS with nervous system involvement were analyzed retrospectively with autoimmune antibody test,cerebrespinal fluid examination and Schivmer's test,six pSS patients with peripheral nervous system lesions (PNS-pSS) were examined with electromyography and nerve conduction velocity and four pSS patients with central nervous system lesions (CNS-pSS) were examined with cranial MRI to analyze their clinical and pathological characteristics.Salivary gland biopsy was performed for all the ten patients,sural nerve biopsy for those with PNS-pSS and brain biopsy or spinal autopsy for those with CNS-pSS.Results Clinically,six patients with PNS-pSS presented symmetrical sensory-motor peripheral neuropathy or pure sensory peripheral neuropathy and four patients with CNS-pSS presented multiple sclerosis,subacute transverse myelitis,encephalitis,pituitary stroke and acute meningitis with varied lesions in the myelin sheath and axon by electromyography and motor nerve conduction velocity.Cranial MRI showed lesions in the white matter of the brain,spinal cord and abnormal signals in the pituitary.Pathologically,sural nerve biopsy showed typical vasculitis and nonvasculitis characterized as degeneration of the axon and myelin sheath,salivary acinar gland biopsy showed its atrophy and infiltration with lymphocytes,and brain biopsy showed demyelination of the brain and spinal white matter,as well as infiltration of lymphocytes surrounding the veins,typical vasculitis and bleeding and necrosis of the pituitary.Conclusions Both central and peripheral nervous systems can be involved in pSS,with complicated clinical manifestations.Electrophysiology,cerebrospinal fluid tests and histopathological examinations by biopsy are essential in diagnosis for neurological lesions in patients with pSS.Inflammatory reaction of the blood vessels and tissues mediated by cell immunity may be involved in mechanism of its neurological lesions.

Objective To determine the impact of exogenous vascular endothelial growth factor (VEGF) on rat liver function after partial liver transplantation. Methods Male SD rats which had undergone 30% partial liver transplantation were divided into VEGF treatment group (n=60) and control group (n=60). Animals in each group were injected with solution of VEGF or saline, respectively. Serum ALT, AST, and hyaluronic acid (HA) levels were assessed at 0, 12, 24, 72, 168 hours after operation. Results Serum ALT, AST and HA levels were significantly decreased at 72 hours after transplantation in VEGF treatment group compared with controls (t =4.681, 4.252, 2.853, P<0.05). In accordance with liver function, treatment with VEGF minimized the damage to the liver sinusoidal endothelial cells and decreased the infiltration of inflamatory cells. Conclusion Administration of VEGF may improve the liver function of rats after partial liver transplantation by maintaining the integrality of the sinusoidal structure and reducing the inflamation reaction.

Objective To explore the correlation of helicobacter pylori(HP) infection in liver cirrhosis(LC) of different syndrome patterns with blood ammonia level.Methods Two hundred and seventeen LC patients were divided into Hp-positive group and Hp-negative group.The patients were classified into 6 syndrome patterns: liver-Qi stagnation,internal accumulation of water-dampness,stagnation of damp-heat,deficiency of liver and kidney yin,deficiency of spleen and kidney yang,and blood-stasis blocking collaterals.Parameters of blood ammonia level,hepatic function and blood coagulation function were observed in LC patients with different syndrome patterns.Results The Hp infection rate in 217 LC patients was 40.1%,the difference being insignificant in the patients with different syndrome patterns and hepatic function grading.The percentage was in a decreasing sequence in syndrome patterns of stagnation of damp-heat,internal accumulation of water-dampness,blood-stasis blocking collaterals,deficiency of liver and kidney yin,liver-Qi stagnation,deficiency of spleen and kidney yang.The difference of blood ammonia level was significant between Hp-positive group and Hp-negative group(P0.05).Conclusion Hp infection is an important factor of inducing the increase of blood ammonia level in LC.Blood ammonia level increases in LC patients after Hp infection,especially in patients with blood-stasis blocking collaterals.The hepatic function grading is corrected with blood ammonia level,the worse the hepatic function,the higher the blood ammonia level.