Taking Children's Hospital of Fudan University as an example, the paper establishes a patient identity matching model based on the historical treatment data according to features of pediatric patients.It also sets up a uniform indexing mechanism of the his-torical treatment and hospitalization records of the patients by improving the existing business system, providing support patient identity recognition and laying a foundation for establishment of the clinical data center in future.

Objective: To investigate the clinical efficacy of treating insomnia with mind-tranquilizing acupuncture. Method: Sixty-three patients were randomly divided into a treatment group of 32 cases and a control group of 31 cases. The treatment group received mind-tranquilizing acupuncture and the control group, oral depression-relieving and mind-calming herbal granules, for 30 days. Results: The response rate was 87.5% in the treatment group and 77.4% in the control group. There were significant differences in falling-sleep time, lasting period and times of nocturnal waking (P ＜ 0.05) and a very significant difference in dream improvement (P＜ 0.01) between the two groups. There were no significant differences in afterwaking conditions and sleep evaluation (P＞ 0.05). Conclusion: Clinically mind-tranquilizing acupuncture has a better effect on insomnia than depression-relieving and mind-calming herbal granules.

After the connotation of collaborative innovation service was described, the difficulties for libraries to carry out collaborative innovation service were analyzed, including the unbalanced distribution of benefits, difference in its understanding , difficulties for theleading libraryto play its role, and the approaches were put forward for libraries to implement their collaborative innovation service in libraries.

Objective:To observe the therapeutic effect of narrow-band UVB combined with glycyrrhizin compound on chronic eczema. Methods:81 cases of patients with chronic eczema were randomly divided into treatment group 42 cases and the control group 39 cases . The treatment group was treated with narrow-band UVB therapy once every two days combined with compound glycyrrhizin capsules 150 mg,3 times a day;the control group was treated only with compound glycyrrhizin capsules 150 mg,3 times a day. Two groups were treated for 4 weeks altogether. Results:The efficacy rate of therapy group (95%)was obviously better than the control (64%). Conclusion:The narrow-band UVB combined with compound glycyrrhizin treatment is a safe and effective treatment for chronic eczema.

Objective: To compare the unobstructed rate and the changes of the diameter of the transplanted vein after the surgery of autogeneic graft of parallel bi-vein and uni-vein with valves.Methods: 24 New Zealand rabbits were randomly divided into experimental group and control group(each group had 12 rabbits).The inferior vena cava(IVC) was replaced by autogeneic graft of parallel bi-vein(in experimental group) or uni-vein(in control group) with valves.The unobstructed rate and the changes of the diameter of the transplanted vein were observed by color Doppler ultrasound 1 d,1 w,2 w and 4 w after surgery.Results: 1d after surgery the diameter of the vessels and the spectrum of the flow returned to normal except one rabbit.At the time of 1 d,1 w and 2 w after surgery,the unobstructed rate of the transplanted vessels in both groups was 100%.There were 1 rabbit in the experimental group and one in the control group,whose transplanted vein were obstructed 4 w after surgery because of the compression of the adhering zone of peritoneum,which was confirmed by anatomy.No thrombus was observed in the transplanted vein in all animals during the 4 weeks after surgery.Conclusion: The transplanted veins are unobstructed during the 4 weeks after autogeneic graft of parallel bi-vein with valves.The unobstructed rate of the transplanted vein in the experimental group and the control group has no significant difference.The diameter of the transplanted vein has no changes in all animals.

Objective To obtain stable arsenic-resistance cells,the fetal bone marrow mesenchymal stem cells(BMSCs)were exposed to low-level arsenite for 18 weeks. Methods Cells from 4 months fetal bone marrow were cultured in ?-MEM medium to obtain BMSCs.After 24h cytotoxicity test of the fetal BMSCs,we chosed 1?mol/L NaAsO_2 to be the dose under which the cell death-rate was 5%-10%.The fetal BMSCs were exposed to low-level arsenite.MTT was used to detect the survival rate and IC_(50) of arsenic-exposed cells and the control cells,which can reflect the change of arsenic tolerance.A hydride generationatomic fluorescence spectrometry method was used to detect arsenic in the arsenic-resistance cells and the control cells.In order to study the mechanism of arsenic-resistance,we also examined the intracellular GSH and GST content in the arsenic-resistance cells and the control cells. Results The fetal BMSCs were continuously exposed to 1?mol/L NaAsO_2.Parallel cells were cultured in medium without arsenic provided passage-matched control.After the fetal BMSCs were continuously exposed to low level NaAsO_2 for 18 weeks,cells exhibited dramatic resistance to acute arsenite toxicity.Compared to control cells,arsenic-resistance cells showed reduction in arsenic accumulation in cells.The GSH levels and GST activity of arsenic-resistance cells were higher than that of control cells.Conclusion Acquisition of stable arsenic-resistance in BMSCs was available by using cell culture and adding low-level arsenic.Our research established a solid basis for further study about the mechanisms of arsenic-resistance in human being.

Objective:To establish an improved transonic extraction-high performance liquid chromatography(HPLC)method for separating and detecting toosendanin(TSN)in Melia toosendanin extractive.Methods:Transonic alcohol-chloroform extraction method was used to extract TSN from the bark of Melia toosendanin sieb.et zuccc.The content determination of TSN was performed on an Agilent XDB C18 Column(4.6?250 mm,5?m).Elution was performed using 45%acetonitrile-water solution as the mobile phase at a1flow rate of 1ml/min.Detection was performed at 215 nm wavelength.Results:The linear regression equation for peak area-TSN concentration(4.64～88.2?g/ml)was:A=7.616 2C-2.567 7,r=0.999 6;mean recovery of standard substance was 100.83%;RSD of precision test was 1.42%;RSD of stability test was 2.26%.Conclusion:The method is simple,rapid,stable and roducible,which can be used as a method for preparation and identification TSN of Melia toosendanin extractive in laboratory.

Objective To explore the efficacy of large dose of cyclophosphamide and etoposide regimen combined with T cells in vivo in treatment of systemic lupus erythematosus by removing pretreatment autologous transplantation. Methods 21 cases with Systemic lupus erythematosus collected in the First Afifliated Hospital of Henan University of Science and Technology were used as subject, bone marrow of them were obtained and saved in 4℃for 72 h, then pretreatment with high-dose cyclophosphamide and etoposide, the efifcacy of these drugs combined with T cell in vivo in systemic lupus erythematosus were observed and analyzed. Results The time of neutrophil number up to 0.5×109/L was (1.27±0.72) days, platelet up to 20×109/L was (4.50±0.67)days, all patients were successfully transplanted hematopoietic reconstruction. During transplanting, there were 19 cases in fever (90%), 3 cases in positive blood culture, 6 cases in liver function damage (30%), 12 cases of electrolyte disturbance (57%), 21 cases of oral ulcer (100%). 21 patients were enrolled, 18 patients with clinical symptoms were signiifcantly improved, the efifciency rate was 85%. Conclusion Large dose of cyclophosphamide and etoposide regimen combined with in vivo T cell depletion pretreated autologous transplantation for the treatment of systemic lupus erythematosus has good effect.

Objective:To compare the value of color Doppler ultrasound(CDU),DSA,CTA and MRA in the detection of the tramatic femoral pseudoaneurysm(PA) of rabbit.Methods:Femoral pseudoaneurysms were tried established in 20 New Zealand rabbits by microsurgical technique.CDU,DSA,CTA and MRA were performed in all rabbits on 12 days after surgery and the findings of them were compared with the results of autopsy.Results:Models were made successfully in 16 rabbits.Eleven out of the 16 rabbits had developed thrombus in PA.The shown rates of the PA-body in CDU,DSA,CTA and MRA were all 100%.The shown rate of the PA-supply artery was 81.25%(13/16) in CDU and was 100% in all other three methods.The shown rates of the PA-neck were 81.25%(13/16)、87.5%(14/16)、37.50%(6/16)and 31.25%(5/16)while the shown rates of the thrombus in PA were 81.82%(9/11),18.18%(2/11),18.18%(2/11) and 36.36%(4/11) in CDU,DSA,CTA and MRA,respectively. Conclusions:Thereare no significant difference in showing the PA-body and PA-supply artery in CDU,DSA,CTA and MRA. In showing the PA-neck,CDU and DSA are superior to CTA and MRA,while CDU is superior to DSA,CTA and MRA in showing the thrombus in PA.In addition,the blood dynamic changes of PA-body,PA-neck and PA-supply artery can be studied timely by CDU,so CDU has high value in the follow up of the patient with PA.

Practical education is one of the most important ways to cultivate the medical personnel who has the ability and moral integrity at higher medical schools. To set up a long-term mechanism of practice of educating people, steps should be taken to fully understand the important sense of the times of practical education to medical students, research and construct the scientific system of practical education and protection mechanism, and to carry out diversity of evaluation and assessment.